| Intein Name |
Tag Pol-1 (Tsp-TY Pol-1) |
| Prototype Allele |
Pko Pol-1 |
| Extein Name |
DNA polymerase (alpha or family B) |
| Intein Class |
Experimental |
| Organism Name |
Thermococcus aggregans |
| Organism Description |
Thermophile, taxon:110163 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
R409 |
| Insert Site Comments |
pol-a, Pol Motif A |
| Intein Size (aa) |
360 |
| Intein N-terminal |
WENIAYLDFR/C |
| Intein C-terminal |
HN/SLYPSIIVTH |
| Accession No. |
CAA73475.1 in NCBI/protein |
| Intein aa Sequence |
>gi|2293389|emb|CAA73475.1| Tag Pol-1 (Tsp-TY Pol-1) intein (including -1 and +1 extein residues)
RCHPADTKVIVKGKGIVNISDVKEGDYILGIDGWQRVKKVWKYHYEGKLININGLKCTPNHKVPVVTEND
RQTRIRDSLAKSFLSGKVKGKIITTKLFEKIAEFEKNKPSEEEILKGELSGIILAEGTLLRKDIEYFDSS
RGKKRISHQYRVEITIGENEKELLERILYIFDKLFGIRPSVKKKGDTNALKITTAKKAVYLQIEELLKNI
ESLYAPAVLRGFFERDATVNKIRSTIVVTQGTNNKWKIDIVAKLLDSLGIPYSRYEYKYIENGKELTKHI
LEITGRDGLILFQTLVGFISSEKNEALEKAIEVREMNRLKNNSFYNLSTFEVSSEYYKGEVYDLTLEGNP
YYFANGILTHNS |
| Block A |
CHPADTKVIVKGK 13 |
| Block B |
INGLKCTPNHKVPV 64 |
| Block C |
LSGIILAEG 126 |
| Block D |
LKNIESLY 213 |
| Block E |
VLRGFFERDA 226 |
| Block H |
TNNKWKIDIVAKLLDSLGI 259 |
| Block F |
EVYDLTL**EGNPYYF 352 |
| Block G |
NGILTHNS 361 |
| Initially Contributed by |
F. Perler for F. Niehaus |
| Contributor's Address |
Technical University Hamburg-Harburg
Biotechnology I, Technical Microbiology
Denickestr. 15, 21073 Hamburg, FRG |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
|
| Comments |
This DNA Pol contains inteins in the 3 most conserved alpha Pol Regions (Perler).
Formerly called Thermococcus spp. TY and Tsp-TY Pol-1. |
| Date Submitted |
05/06/1997 |
| References |
Niehaus 1997 |
