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InBase, The Intein Database: Protein Splicing Bibliography

InBase Reference: Perler, F. B. (2002). InBase, the Intein Database. Nucleic Acids Res. 30, 383-384.

If you think a paper is missing from InBase, please send the reference and PDF (if possible) to the curator at

The following annotations have been used in the InBase Bibliography. Please inform the curator if you notice a missing or incorrect annotation.

Select: Annotation Key:
(APP) Application
(CPS) Conditional Protein Splicing (cis or trans)
(CYCL) Protein/peptide backbone cyclization
(EN) Homing endonuclease
(EPL) IPL, intein-mediated expressed protein ligation
(INT) References describing inteins or sequenced genomes containing inteins
(NEB) Papers from NEB
(NMR) Segmental labeling for NMR or other types of analysis
(PUR) Intein vector systems and examples of intein-mediated purifications
(REL) Related area of interest
(REV) Review, letter, note or commentary
(Structure) Structural papers of inteins or other proteins prepared using intein technology
(TAG) Addition of binding or detection tag
(TS) Trans-splicing
Recent publications are in Red

Sorting order:

1.  (APP) (PUR) Abbing, A.; Blaschke, U. K.; Grein, S.; Kretschmar, M.; Stark, C. M.; Thies, M. J.; Walter, J.; Weigand, M.; Woith, D. C.; Hess, J.; Reiser, C. O. (2004) Efficient intracellular delivery of a protein and a low molecular weight substance via recombinant polyomavirus-like particles. J Biol Chem 279(26): 27410-21. PubMed ID: 15102846
2.  (INT) (REL) (REV) Abdelhaleem, M. (2010) Helicases: an overview. Methods Mol Biol 587: 1-12. PubMed ID: 20225138
3.  (APP) (CYCL) (REV) Abel-Santos, E.; Scott, C. P.; Benkovic, S. J. (2003) Use of inteins for the in vivo production of stable cyclic peptide libraries in E. coli. Methods Mol Biol 205: 281-94. PubMed ID: 12491894
4.  (APP) (PUR) Abhyankar, M. M.; Zzaman, S.; Bastia, D. (2003) Reconstitution of R6K DNA replication in vitro using 22 purified proteins. J Biol Chem 278(46): 45476-84. PubMed ID: 12970346
5.  (APP) (CPS) (INT) (NEB) Adam, E.; Perler, F. B. (2002) Development of a positive genetic selection system for inhibition of protein splicing using mycobacterial inteins in Escherichia coli DNA gyrase subunit A. J Mol Microbiol Biotechnol 4(5): 479-87. PubMed ID: 12432958
6.  (INT) (NEB) (REV) Adams, M. W.; Perler, F. B.; Kelly, R. M. (1995) Extremozymes: expanding the limits of biocatalysis. Biotechnology (N Y) 13(7): 662-8. PubMed ID: 9634802
7.  (REL) (REV) Adamska, M.; Matus, D. Q.; Adamski, M.; Green, K.; Rokhsar, D. S.; Martindale, M. Q.; Degnan, B. M. (2007) The evolutionary origin of hedgehog proteins. Curr Biol 17(19): R836-7. PubMed ID: 17925209
8.  (REL) Agarwal, S. M.; Srivastava, P. K. (2009) Human intronless disease associated genes are slowly evolving. BMB Rep 42(6): 356-60. PubMed ID: 19558794
9.  (APP) (EPL) (TAG) Ajish Kumar, K. S.; Haj-Yahya, M.; Olschewski, D.; Lashuel, H. A.; Brik, A. (2009) Highly efficient and chemoselective peptide ubiquitylation. Angew Chem Int Ed Engl 48(43): 8090-4. PubMed ID: 19780082
10.  (APP) (PUR) (TS) Al-Ali, H.; Ragan, T. J.; Gao, X.; Harris, T. K. (2007) Reconstitution of modular PDK1 functions on trans-splicing of the regulatory PH and catalytic kinase domains. Bioconjug Chem 18(4): 1294-302. PubMed ID: 17500509
11.  (REL) (Structure) Albert, A.; Dhanaraj, V.; Genschel, U.; Khan, G.; Ramjee, M. K.; Pulido, R.; Sibanda, B. L.; von Delft, F.; Witty, M.; Blundell, T. L.; Smith, A. G.; Abell, C. (1998) Crystal structure of aspartate decarboxylase at 2.2 A resolution provides evidence for an ester in protein self-processing. Nat Struct Biol 5(4): 289-93. PubMed ID: 9546220
12.  (EN) (REV) Alexander, Rebecca W. (2002) Measure for measure: a zinc finger ruler. Trends in Biochemical Sciences 27(8): 390.
13.  (APP) (EPL) (TAG) Alexandrov, K.; Heinemann, I.; Durek, T.; Sidorovitch, V.; Goody, R. S.; Waldmann, H. (2002) Intein-mediated synthesis of geranylgeranylated Rab7 protein in vitro. J Am Chem Soc 124(20): 5648-9. PubMed ID: 12010032
14.  (APP) (EPL) (REL) Alves, D. A.; Esser, D.; Broadbridge, R. J.; Beevers, A. P. G.; Chapman, C. P.; Winsor, C. E.; Betley, J. R. (2003) Synthesis and use of a pseudo-cysteine for native chemical ligation. Journal of Peptide Science 9(4): 221-228.
15.  (APP) (REV) Amitai, G.; Pietrokovski, S. (1999) Fine-tuning an engineered intein. Nat Biotechnol 17(9): 854-5. PubMed ID: 10471922
16.  (INT) (REL) Amitai, G.; Belenkiy, O.; Dassa, B.; Shainskaya, A.; Pietrokovski, S. (2003) Distribution and function of new bacterial intein-like protein domains. Mol Microbiol 47(1): 61-73. PubMed ID: 12492854
17.  (INT) Amitai, G.; Dassa, B.; Pietrokovski, S. (2004) Protein splicing of inteins with atypical glutamine and aspartate C-terminal residues. J Biol Chem 279(5): 3121-31. PubMed ID: 14593103
18.  (APP) (INT) Amitai, G.; Callahan, B. P.; Stanger, M. J.; Belfort, G.; Belfort, M. (2009) Modulation of intein activity by its neighboring extein substrates. Proc Natl Acad Sci U S A 106(27): 11005-10. PubMed ID: 19541659
19.  (APP) (EPL) Anderson, L. L.; Marshall, G. R.; Baranski, T. J. (2005) Expressed protein ligation to study protein interactions: semi-synthesis of the G-protein alpha subunit. Protein Pept Lett 12(8): 783-7. PubMed ID: 16305549
20.  (APP) (EPL) Anderson, L. L.; Marshall, G. R.; Crocker, E.; Smith, S. O.; Baranski, T. J. (2005B) Motion of carboxyl terminus of Galpha is restricted upon G protein activation. A solution NMR study using semisynthetic Galpha subunits. J Biol Chem 280(35): 31019-26. PubMed ID: 15983037
21.  (APP) (CPS) (TS) Ando, T.; Tsukiji, S.; Tanaka, T.; Nagamune, T. (2007) Construction of a small-molecule-integrated semisynthetic split intein for in vivo protein ligation. Chem Commun (Camb) (47): 4995-7. PubMed ID: 18049731
22.  (INT) (REV) Anraku, Y.; Hirata, R. (1994) Protozyme: emerging evidence in nature. J Biochem (Tokyo) 115(2): 175-8. PubMed ID: 8206863
23.  (INT) (REV) Anraku, Y. (1997) [The discovery and molecular mechanism of protein splicing]. Tanpakushitsu Kakusan Koso 42(15): 2483-97. PubMed ID: 9391338
24.  (INT) (REV) Anraku, Y. (1997B) Protein splicing: its chemistry and biology. Genes Cells 2(6): 359-67. PubMed ID: 9286854
25.  (INT) (REV) Anraku, Y.; Mizutani, R.; Satow, Y. (2005) Protein splicing: its discovery and structural insight into novel chemical mechanisms. IUBMB Life 57(8): 563-74. PubMed ID: 16118114
26.  (INT) (REV) Anraku, Y.; Satow, Y. (2009) Reflections on protein splicing: structures, functions and mechanisms. Proc Jpn Acad Ser B Phys Biol Sci 85(9): 409-21. PubMed ID: 19907126
27.  (APP) (TAG) (TS) Appleby, J. H.; Zhou, K.; Volkmann, G.; Liu, X. Q. (2009) Novel split-intein for trans-splicing synthetic peptide onto C-terminus of protein. J Biol Chem 284(10): 6094-6199. PubMed ID: 19136555
28.  (APP) (CPS) (TS) Aranko, A. S.; Zuger, S.; Buchinger, E.; Iwai, H. (2009) In vivo and in vitro protein ligation by naturally occurring and engineered split DnaE inteins. PLoS ONE 4(4): e5185. PubMed ID: 19365564
29.  (INT) Argueso, J. L.; Carazzolle, M. F.; Mieczkowski, P. A.; Duarte, F. M.; Netto, O. V.; Missawa, S. K.; Galzerani, F.; Costa, G. G.; Vidal, R. O.; Noronha, M. F.; Dominska, M.; Andrietta, M. G.; Andrietta, S. R.; Cunha, A. F.; Gomes, L. H.; Tavares, F. C.; Alcarde, A. R.; Dietrich, F. S.; McCusker, J. H.; Petes, T. D.; Pereira, G. A. (2009) Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol production. Genome Res 19(12): 2258-70. PubMed ID: 19812109
30.  (APP) (EPL) Arnold, U.; Hinderaker, M. P.; Nilsson, B. L.; Huck, B. R.; Gellman, S. H.; Raines, R. T. (2002) Protein prosthesis: a semisynthetic enzyme with a beta-peptide reverse turn. J Am Chem Soc 124(29): 8522-3. PubMed ID: 12121081
31.  (APP) (EPL) Arnold, U.; Hinderaker, M. P.; Raines, R. T. (2002B) Semisynthesis of ribonuclease A using intein-mediated protein ligation. ScientificWorldJournal 2: 1823-7. PubMed ID: 12806173
32.  (APP) (EPL) (REV) Arnold, U. (2009) Incorporation of non-natural modules into proteins: structural features beyond the genetic code. Biotechnol Lett 31(8): 1129-39. PubMed ID: 19404746
33.  (INT) ASBMB (2010) Oh Little Intein of Bethlehem: Splicing of the Mycobacteriophage Bethlehem DnaB Intein. Identification of a New Mechanistic Class of Inteins That Contain an Obligate Block F Nucleophile. J Biol Chem 285(4): p. e99910.
34.  (REL) Aspock, G.; Kagoshima, H.; Niklaus, G.; Burglin, T. R. (1999) Caenorhabditis elegans has scores of hedgehog-related genes: sequence and expression analysis. Genome Res 9(10): 909-23. PubMed ID: 10523520
35.  (APP) (CYCL) (EPL) (REL) Austin, J.; Wang, W.; Puttamadappa, S.; Shekhtman, A.; Camarero, J. A. (2009) Biosynthesis and biological screening of a genetically encoded library based on the cyclotide MCoTI-I. Chembiochem 10(16): 2663-70. PubMed ID: 19780078
36.  (APP) (EPL) Ayers, B.; Blaschke, U. K.; Camarero, J. A.; Cotton, G. J.; Holford, M.; Muir, T. W. (1999) Introduction of unnatural amino acids into proteins using expressed protein ligation. Biopolymers 51(5): 343-54. PubMed ID: 10685045
37.  (INT) Bae, H.; Kim, K. P.; Lee, J. I.; Song, J. G.; Kil, E. J.; Kim, J. S.; Kwon, S. T. (2009) Characterization of DNA polymerase from the hyperthermophilic archaeon Thermococcus marinus and its application to PCR. Extremophiles 13(4): 657-67. PubMed ID: 19412677
38.  (EN) (INT) Bae, H.; Kim, K. P.; Song, J. M.; Kim, J. H.; Yang, J. S.; Kwon, S. T. (2009B) Characterization of intein homing endonuclease encoded in the DNA polymerase gene of Thermococcus marinus. FEMS Microbiol Lett 297(2): 180-8. PubMed ID: 19634205
39.  (REL) (REV) Bailey, E. C.; Scott, M. P.; Johnson, R. L. (2000) Hedgehog signaling in animal development and human disease. Ernst Schering Res Found Workshop. (29): 211-35.. PubMed ID: 10943312
40.  (EN) Bakhrat, A.; Jurica, M. S.; Stoddard, B. L.; Raveh, D. (2004) Homology modeling and mutational analysis of HO endonuclease of yeast. Genetics 166(2): 721-8. PubMed ID: 15020462
41.  (EN) (REL) Bakhrat, A.; Baranes, K.; Krichevsky, O.; Rom, I.; Schlenstedt, G.; Pietrokovski, S.; Raveh, D. (2006) Nuclear import of HO endonuclease utilizes two nuclear localization signals and four importins of the ribosomal import system. J Biol Chem 281(18): 12218-26. PubMed ID: 16507575
42.  (INT) Baliga, N. S.; Bonneau, R.; Facciotti, M. T.; Pan, M.; Glusman, G.; Deutsch, E. W.; Shannon, P.; Chiu, Y.; Weng, R. S.; Gan, R. R.; Hung, P.; Date, S. V.; Marcotte, E.; Hood, L.; Ng, W. V. (2004) Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea. Genome Res 14(11): 2221-34. PubMed ID: 15520287
43.  (APP) (PUR) Banki, M. R.; Gerngross, T. U.; Wood, D. W. (2005) Novel and economical purification of recombinant proteins: intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association. Protein Sci 14(6): 1387-95. PubMed ID: 15883185
44.  (APP) (PUR) (REL) Banki, M. R.; Feng, L.; Wood, D. W. (2005B) Simple bioseparations using self-cleaving elastin-like polypeptide tags. Nat Methods 2(9): 659-61. PubMed ID: 16074986
45.  (APP) (PUR) (REV) Banki, M. R.; Wood, D. W. (2005C) Inteins and affinity resin substitutes for protein purification and scale up. Microb Cell Fact 4: 32. PubMed ID: 16283936
46.  (INT) Bao, X.; Carris, L. M.; Huang, G.; Luo, J.; Liu, Y.; Castlebury, L. A. (2010) Tilletia puccinelliae, a new species of reticulate-spored bunt fungus infecting Puccinellia distans. Mycologia 102(3): 613-23. PubMed ID: 20524594
47.  (APP) (EPL) (TAG) Bark, S. J.; Schmid, S.; Hahn, K. M. (2000) A highly efficient method for site-specific modification of unprotected peptides after chemical synthesis. Journal of the American Chemical Society 122(15): 3567-3573.
48.  (APP) (PUR) Barnard, G. C.; McCool, J. D.; Wood, D. W.; Gerngross, T. U. (2005) Integrated recombinant protein expression and purification platform based on Ralstonia eutropha. Appl Environ Microbiol 71(10): 5735-42. PubMed ID: 16204482
49.  (APP) (EPL) Barry, A. N.; Blackburn, N. J. (2008) A selenocysteine variant of the human copper chaperone for superoxide dismutase. A Se-XAS probe of cluster composition at the domain 3-domain 3 dimer interface. Biochemistry 47(17): 4916-28. PubMed ID: 18393442
50.  (APP) (EPL) Barry, A. N.; Clark, K. M.; Otoikhian, A.; van der Donk, W. A.; Blackburn, N. J. (2008B) Selenocysteine Positional Variants Reveal Contributions to Copper Binding from Cysteine Residues in Domains 2 and 3 of Human Copper Chaperone for Superoxide Dismutase. Biochemistry 47(49): 13074-13083. PubMed ID: 19007184
51.  (APP) (EPL) (Structure) Baskaran, S.; Roach, P. J.; DePaoli-Roach, A. A.; Hurley, T. D. (2010) Structural basis for glucose-6-phosphate activation of glycogen synthase. Proc Natl Acad Sci U S A 107(41): 17563-8. PubMed ID: 20876143
52.  (APP) (EPL) (PUR) Bastings, M. M.; van Baal, I.; Meijer, E. W.; Merkx, M. (2008) One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester. BMC Biotechnol 8(1): 76. PubMed ID: 18828922
53.  (APP) (PUR) Baumann, R. G.; Black, L. W. (2003) Isolation and characterization of T4 bacteriophage gp17 terminase, a large subunit multimer with enhanced ATPase activity. J Biol Chem 278(7): 4618-27. PubMed ID: 12466275
54.  (APP) (PUR) Bayrhuber, M.; Graf, R.; Ferber, M.; Zweckstetter, M.; Imperial, J.; Garrett, J. E.; Olivera, B. M.; Terlau, H.; Becker, S. (2006) Production of recombinant Conkunitzin-S1 in Escherichia coli. Protein Expr Purif 47(2): 640-4. PubMed ID: 16542850
55.  (APP) (REL) Bazirgan, O. A.; Garza, R. M.; Hampton, R. Y. (2006) Determinants of RING-E2 fidelity for Hrd1p, a membrane-anchored ubiquitin ligase. J Biol Chem 281(51): 38989-9001. PubMed ID: 17035235
56.  (REL) (REV) Beachy, P. A.; Cooper, M. K.; Young, K. E.; von Kessler, D. P.; Park, W. J.; Hall, T. M.; Leahy, D. J.; Porter, J. A. (1997) Multiple roles of cholesterol in hedgehog protein biogenesis and signaling. Cold Spring Harb Symp Quant Biol 62: 191-204. PubMed ID: 9598352
57.  (INT) (REL) (REV) Becker, Y. (1998) Molecular evolution of viruses--Past and Present, Part 2--An introduction. Virus Genes 16(1): 7-11. PubMed ID: 9562887
58.  (APP) (EPL) Becker, C. F.; Lausecker, K.; Balog, M.; Kalai, T.; Hideg, K.; Steinhoff, H. J.; Engelhard, M. (2005) Incorporation of spin-labelled amino acids into proteins. Magn Reson Chem 43: S34-9. PubMed ID: 16235213
59.  (APP) (EPL) (TAG) Becker, C. F.; Seidel, R.; Jahnz, M.; Bacia, K.; Niederhausen, T.; Alexandrov, K.; Schwille, P.; Goody, R. S.; Engelhard, M. (2006) C-terminal fluorescence labeling of proteins for interaction studies on the single-molecule level. Chembiochem 7(6): 891-5. PubMed ID: 16607669
60.  (APP) (EPL) Becker, C. F. W.; Liu, X.; Olschewski, D.; Castelli, R.; Seidel, R.; Seeberger, P. H. (2008) Semisynthesis of a glycosylphosphatidylinositol-anchored prion protein. Angewandte Chemie - International Edition 47(43): 8215-8219.
61.  (REL) (REV) Begley, T. P.; Xi, J.; Kinsland, C.; Taylor, S.; McLafferty, F. (1999) The enzymology of sulfur activation during thiamin and biotin biosynthesis. Curr Opin Chem Biol 3(5): 623-9. PubMed ID: 10508664
62.  (APP) (REV) Beguin, P. (1999) Hybrid enzymes. Curr Opin Biotechnol 10(4): 336-40. PubMed ID: 10449312
63.  (INT) Belanger, A. S.; Brouard, J. S.; Charlebois, P.; Otis, C.; Lemieux, C.; Turmel, M. (2006) Distinctive architecture of the chloroplast genome in the chlorophycean green alga Stigeoclonium helveticum. Mol Genet Genomics 276(5): 464-77. PubMed ID: 16944205
64.  (REL) (REV) Belfort, M.; Perlman, P. S. (1995) Mechanisms of intron mobility. J Biol Chem 270(51): 30237-40. PubMed ID: 8530436
65.  (INT) (REV) Belfort, M.; Reaban, M. E.; Coetzee, T.; Dalgaard, J. Z. (1995B) Prokaryotic introns and inteins: a panoply of form and function. J Bacteriol 177(14): 3897-903. PubMed ID: 7608058
66.  (EN) (REV) Belfort, M.; Roberts, R. J. (1997) Homing endonucleases: keeping the house in order. Nucleic Acids Res 25(17): 3379-88. PubMed ID: 9254693
67.  (EN) (REL) (REV) Belfort, M. (2003) Two for the price of one: a bifunctional intron-encoded DNA endonuclease-RNA maturase. Genes Dev 17(23): 2860-3. PubMed ID: 14665667
68.  (APP) (EPL) (TAG) Bellmann-Sickert, K.; Baumann, L.; Beck-Sickinger, A. G. (2010) Selective labelling of stromal cell-derived factor 1alpha with carboxyfluorescein to study receptor internalisation. J Pept Sci 16(10): 568-74. PubMed ID: 20862724
69.  (APP) (REV) Benkovic, S. J.; Walsh, C. T. (1997) Research highlights at the chemistry/biology interface. Curr Opin Chem Biol 1(3): 289-91. PubMed ID: 9667881
70.  (APP) (PUR) Berndt, C.; Lillig, C. H.; Wollenberg, M.; Bill, E.; Mansilla, M. C.; de Mendoza, D.; Seidler, A.; Schwenn, J. D. (2004) Characterization and reconstitution of a 4Fe-4S adenylyl sulfate/phosphoadenylyl sulfate reductase from Bacillus subtilis. J Biol Chem 279(9): 7850-5. PubMed ID: 14627706
71.  (APP) (EPL) (REV) Berrade, L.; Camarero, J. A. (2009) Expressed protein ligation: a resourceful tool to study protein structure and function. Cell Mol Life Sci 66(24): 3909-22. PubMed ID: 19685006
72.  (APP) (CPS) (TS) Berrade, L.; Kwon, Y.; Camarero, J. A. (2010) Photomodulation of protein trans-splicing through backbone photocaging of the DnaE split intein. Chembiochem 11(10): 1368-72. PubMed ID: 20512791
73.  (APP) (EPL) Berry, S. M.; Gieselman, M. D.; Nilges, M. J.; van Der Donk, W. A.; Lu, Y. (2002) An engineered azurin variant containing a selenocysteine copper ligand. J Am Chem Soc 124(10): 2084-5. PubMed ID: 11878940
74.  (APP) (EPL) Berry, S. M.; Ralle, M.; Low, D. W.; Blackburn, N. J.; Lu, Y. (2003) Probing the role of axial methionine in the blue copper center of azurin with unnatural amino acids. J Am Chem Soc 125(29): 8760-8. PubMed ID: 12862470
75.  (REL) Bezerra, W. M.; Carvalho, C. P.; Moreira Rde, A.; Grangeiro, T. B. (2006) Establishment of a heterologous system for the expression of Canavalia brasiliensis lectin: a model for the study of protein splicing. Genet Mol Res. 5(1): 216-23. PubMed ID: 16755512
76.  (APP) (NEB) (PUR) (REL) Bitinaite, J.; Rubino, M.; Varma, K. H.; Schildkraut, I.; Vaisvila, R.; Vaiskunaite, R. (2007) USER friendly DNA engineering and cloning method by uracil excision. Nucleic Acids Res 35(6): 1992-2002. PubMed ID: 17341463
77.  (APP) (PUR) Bjorklund, M.; Valtanen, H.; Savilahti, H.; Koivunen, E. (2003) Use of intein-directed peptide biosynthesis to improve serum stability and bioactivity of a gelatinase inhibitory peptide. Comb Chem High Throughput Screen 6(1): 29-35. PubMed ID: 12570750
78.  (INT) Bjornsdottir, S. H.; Blondal, T.; Hreggvidsson, G. O.; Eggertsson, G.; Petursdottir, S.; Hjorleifsdottir, S.; Thorbjarnardottir, S. H.; Kristjansson, J. K. (2006) Rhodothermus marinus: physiology and molecular biology. Extremophiles 10(1): 1-16. PubMed ID: 16075163
79.  (INT) Blackwood, K. S.; He, C.; Gunton, J.; Turenne, C. Y.; Wolfe, J.; Kabani, A. M. (2000) Evaluation of recA sequences for identification of Mycobacterium species. J Clin Microbiol 38(8): 2846-52. PubMed ID: 10921937
80.  (APP) (REV) Blank, K.; Lindner, P.; Diefenbach, B.; Pluckthun, A. (2002) Self-immobilizing recombinant antibody fragments for immunoaffinity chromatography: generic, parallel, and scalable protein purification. Protein Expr Purif 24(2): 313-22. PubMed ID: 11858727
81.  (APP) (EPL) Blaschke, U. K.; Silberstein, J.; Muir, T. W. (2000) Protein engineering by expressed protein ligation. Methods Enzymol 328: 478-96. PubMed ID: 11075362
82.  (APP) (EPL) (NMR) (REV) (Structure) Blaschke, U. K.; Cotton, G.J.; Muir, T. W. (2000B) Synthesis of multi-domain proteins using expressed protein ligation: strategies for segmental labeling of internal regions.. Tetrahedron 56: 9461-9470.
83.  (APP) (EPL) (PUR) Bockhorn, J. J.; Lazar, K. L.; Gasser, A. J.; Luther, L. M.; Qahwash, I. M.; Chopra, N.; Meredith, S. C. (2010) Novel semisynthetic method for generating full length beta-amyloid peptides. Biopolymers 94(4): 511-20. PubMed ID: 20593467
84.  (EN) (INT) Bokor, A. A.; van Kan, J. A.; Poulter, R. T. (2010) Sexual mating of Botrytis cinerea illustrates PRP8 intein HEG activity. Fungal Genet Biol 47(4): 392-8. PubMed ID: 20093192
85.  (INT) Bolhuis, H.; Palm, P.; Wende, A.; Falb, M.; Rampp, M.; Rodriguez-Valera, F.; Pfeiffer, F.; Oesterhelt, D. (2006) The genome of the square archaeon Haloquadratum walsbyi : life at the limits of water activity. BMC Genomics 7: 169. PubMed ID: 16820047
86.  (REL) (Structure) Bompard-Gilles, C.; Villeret, V.; Fanuel, L.; Joris, B.; Frere, J. M.; Van Beeumen, J. (1999) Crystallization and preliminary X-ray analysis of a new L-aminopeptidase-D-amidase/D-esterase activated by a Gly-Ser peptide bond hydrolysis. Acta Crystallogr D Biol Crystallogr 55 ( Pt 3): 699-701. PubMed ID: 10089474
87.  (APP) (PUR) (Structure) Bompard-Gilles, C.; Villeret, V.; Davies, G. J.; Fanuel, L.; Joris, B.; Frere, J. M.; Van Beeumen, J. (2000) A new variant of the Ntn hydrolase fold revealed by the crystal structure of L-aminopeptidase D-ala-esterase/amidase from Ochrobactrum anthropi. Structure 8(2): 153-62. PubMed ID: 10673442
88.  (APP) (PUR) Bonsager, B. C.; Praetorius-Ibba, M.; Nielsen, P. K.; Svensson, B. (2003) Purification and characterization of the beta-trefoil fold protein barley alpha-amylase/subtilisin inhibitor overexpressed in Escherichia coli. Protein Expr Purif 30(2): 185-93. PubMed ID: 12880767
89.  (APP) (PUR) Bonsager, B. C.; Nielsen, P. K.; Abou Hachem, M.; Fukuda, K.; Praetorius-Ibba, M.; Svensson, B. (2005) Mutational analysis of target enzyme recognition of the beta-trefoil fold barley alpha-amylase/subtilisin inhibitor. J Biol Chem 280(15): 14855-64. PubMed ID: 15657043
90.  (APP) (EPL) (REV) Borgia, J. A.; Fields, G. B. (2000) Chemical synthesis of proteins. Trends Biotechnol 18(6): 243-51. PubMed ID: 10802559
91.  (REL) (REV) Borissenko, L.; Groll, M. (2007) Diversity of proteasomal missions: fine tuning of the immune response. Biol Chem 388(9): 947-55. PubMed ID: 17696779
92.  (APP) (NMR) (REV) (Structure) Borman, S. (1999) Scaling Up Protein NMR: Segmental labeling technique improves access to 3-D structures of large proteins. Chem & Eng News February 15: 65-67.
93.  (APP) (CPS) (REV) Borman, S. (2002) Controlled Protein Splicing. Chem & Eng News 13: 289.
94.  (APP) (EPL) Borodovsky, A.; Ovaa, H.; Kolli, N.; Gan-Erdene, T.; Wilkinson, K. D.; Ploegh, H. L.; Kessler, B. M. (2002) Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family. Chem Biol 9(10): 1149-59. PubMed ID: 12401499
95.  (APP) (NMR) (REV) (Structure) Bott, R.; Boelens, R. (1999) The role of high-resolution structural studies in the development of commercial enzymes. Curr Opin Biotechnol 10(4): 391-7. PubMed ID: 10449323
96.  (INT) (NEB) Brace, L. E.; Southworth, M. W.; Tori, K.; Cushing, M. L.; Perler, F. (2010) The Deinococcus radiodurans Snf2 intein caught in the act: detection of the Class 3 intein signature Block F branched intermediate. Protein Sci 19(8): 1525-33. PubMed ID: 20521254
97.  (APP) (PUR) Bradley, L. H.; Kleiner, R. E.; Wang, A. F.; Hecht, M. H.; Wood, D. W. (2005) An intein-based genetic selection allows the construction of a high-quality library of binary patterned de novo protein sequences. Protein Eng Des Sel 18(4): 201-7. PubMed ID: 15849217
98.  (APP) (PUR) Brandt, J. J.; Chatwood, L. L.; Crowder, M. W. (2000) Analysis of three overexpression systems for VanX, the Zinc(II) dipeptidase required for high-level vancomycin resistance in bacteria. Protein Expr Purif 20(2): 300-7. PubMed ID: 11049753
99.  (EN) (INT) Bremer, M. C.; Gimble, F. S.; Thorner, J.; Smith, C. L. (1992) VDE endonuclease cleaves Saccharomyces cerevisiae genomic DNA at a single site: physical mapping of the VMA1 gene. Nucleic Acids Res 20(20): 5484. PubMed ID: 1437572
100.  (REL) Brennan, T. V.; Clarke, S. (1993) Spontaneous degradation of polypeptides at aspartyl and asparaginyl residues: effects of the solvent dielectric. Protein Sci 2(3): 331-8. PubMed ID: 8453372
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103.  (APP) (EN) Brenneman, M.; Gimble, F. S.; Wilson, J. H. (1996) Stimulation of intrachromosomal homologous recombination in human cells by electroporation with site-specific endonucleases. Proc Natl Acad Sci U S A 93(8): 3608-12. PubMed ID: 8622983
104.  (APP) (CPS) (TS) Brenzel, S.; Mootz, H. D. (2005) Design of an intein that can be inhibited with a small molecule ligand. J Am Chem Soc 127(12): 4176-7. PubMed ID: 15783192
105.  (APP) (CPS) (INT) (TS) Brenzel, S.; Kurpiers, T.; Mootz, H. D. (2006) Engineering Artificially Split Inteins for Applications in Protein Chemistry: Biochemical Characterization of the Split Ssp DnaB Intein and Comparison to the Split Sce VMA Intein. Biochemistry 45(6): 1571-8. PubMed ID: 16460004
106.  (APP) (TS) Brenzel, S.; Cebi, M.; Reiss, P.; Koert, U.; Mootz, H. D. (2009) Expanding the scope of protein trans-splicing to fragment ligation of an integral membrane protein: towards modulation of porin-based ion channels by chemical modification. Chembiochem 10(6): 983-6. PubMed ID: 19322843
107.  (INT) Brosch, R.; Gordon, S. V.; Garnier, T.; Eiglmeier, K.; Frigui, W.; Valenti, P.; Dos Santos, S.; Duthoy, S.; Lacroix, C.; Garcia-Pelayo, C.; Inwald, J. K.; Golby, P.; Garcia, J. N.; Hewinson, R. G.; Behr, M. A.; Quail, M. A.; Churcher, C.; Barrell, B. G.; Parkhill, J.; Cole, S. T. (2007) Genome plasticity of BCG and impact on vaccine efficacy. Proc Natl Acad Sci U S A 104(13): 5596-601. PubMed ID: 17372194
108.  (REL) (REV) Brugger, K.; Redder, P.; She, Q.; Confalonieri, F.; Zivanovic, Y.; Garrett, R. A. (2002) Mobile elements in archaeal genomes. FEMS Microbiol Lett. 206(2): 131-41.. PubMed ID: 11814653
109.  (INT) Brugger, K.; Chen, L.; Stark, M.; Zibat, A.; Redder, P.; Ruepp, A.; Awayez, M.; She, Q.; Garrett, R. A.; Klenk, H. P. (2007) The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C. Archaea 2(2): 127-35. PubMed ID: 17350933
110.  (APP) (EPL) Brunsveld, L.; Watzke, A.; Durek, T.; Alexandrov, K.; Goody, R. S.; Waldmann, H. (2005) Synthesis of functionalized rab GTPases by a combination of solution- or solid-phase lipopeptide synthesis with expressed protein ligation. Chemistry 11(9): 2756-72. PubMed ID: 15729676
111.  (APP) (EPL) (REL) Brunsveld, L.; Kuhlmann, J.; Alexandrov, K.; Wittinghofer, A.; Goody, R. S.; Waldmann, H. (2006) Lipidated ras and rab peptides and proteins--synthesis, structure, and function. Angew Chem Int Ed Engl 45(40): 6622-46. PubMed ID: 17031879
112.  (APP) (NMR) (TS) Buchinger, E.; Aachmann, F. L.; Aranko, A. S.; Valla, S.; Skjak-Braek, G.; Iwai, H.; Wimmer, R. (2010) Use of protein trans-splicing to produce active and segmentally (2)H, (15)N labeled mannuronan C5-epimerase AlgE4. Protein Sci 19(8): 1534-43. PubMed ID: 20552686
113.  (APP) (EPL) (REV) Budisa, N. (2004) Adding new tools to the arsenal of expressed protein ligation. Chembiochem 5(9): 1176-9. PubMed ID: 15368567
114.  (INT) Buell, C. R.; Joardar, V.; Lindeberg, M.; Selengut, J.; Paulsen, I. T.; Gwinn, M. L.; Dodson, R. J.; Deboy, R. T.; Durkin, A. S.; Kolonay, J. F.; Madupu, R.; Daugherty, S.; Brinkac, L.; Beanan, M. J.; Haft, D. H.; Nelson, W. C.; Davidsen, T.; Zafar, N.; Zhou, L.; Liu, J.; Yuan, Q.; Khouri, H.; Fedorova, N.; Tran, B.; Russell, D.; Berry, K.; Utterback, T.; Van Aken, S. E.; Feldblyum, T. V.; D'Ascenzo, M.; Deng, W. L.; Ramos, A. R.; Alfano, J. R.; Cartinhour, S.; Chatterjee, A. K.; Delaney, T. P.; Lazarowitz, S. G.; Martin, G. B.; Schneider, D. J.; Tang, X.; Bender, C. L.; White, O.; Fraser, C. M.; Collmer, A. (2003) The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000. Proc Natl Acad Sci U S A 100(18): 10181-6. PubMed ID: 12928499
115.  (INT) Bult, C. J.; White, O.; Olsen, G. J.; Zhou, L.; Fleischmann, R. D.; Sutton, G. G.; Blake, J. A.; FitzGerald, L. M.; Clayton, R. A.; Gocayne, J. D.; Kerlavage, A. R.; Dougherty, B. A.; Tomb, J. F.; Adams, M. D.; Reich, C. I.; Overbeek, R.; Kirkness, E. F.; Weinstock, K. G.; Merrick, J. M.; Glodek, A.; Scott, J. L.; Geoghagen, N. S.; Venter, J. C. (1996) Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science 273(5278): 1058-73. PubMed ID: 8688087
116.  (APP) (EPL) (TAG) Burbulis, I.; Yamaguchi, K.; Gordon, A.; Carlson, R.; Brent, R. (2005) Using protein-DNA chimeras to detect and count small numbers of molecules. Nat Methods 2(1): 31-7. PubMed ID: 15782158
117.  (REL) (REV) Burglin, T. R. (1996) Warthog and groundhog, novel families related to hedgehog. Curr Biol 6(9): 1047-50. PubMed ID: 8805384
118.  (REL) (REV) Burglin, T. R. (2008) Evolution of hedgehog and hedgehog-related genes, their origin from Hog proteins in ancestral eukaryotes and discovery of a novel Hint motif. BMC Genomics 9: 127. PubMed ID: 18334026
119.  (REL) Burglin, T. R. (2008B) The Hedgehog protein family. Genome Biol 9(11): 241. PubMed ID: 19040769
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121.  (APP) (NMR) (TS) Busche, A. E.; Aranko, A. S.; Talebzadeh-Farooji, M.; Bernhard, F.; Dotsch, V.; Iwai, H. (2009) Segmental Isotopic Labeling of a Central Domain in a Multidomain Protein by Protein Trans-Splicing Using Only One Robust DnaE Intein. Angew Chem Int Ed Engl 48(33): 6128-6131. PubMed ID: 19591176
122.  (APP) (EPL) (TAG) Buskas, T.; Ingale, S.; Boons, G. J. (2006) Glycopeptides as versatile tools for glycobiology. Glycobiology 16(8): 113R-136R. PubMed ID: 16675547
123.  (APP) (CPS) Buskirk, A. R.; Ong, Y. C.; Gartner, Z. J.; Liu, D. R. (2004) Directed evolution of ligand dependence: small-molecule-activated protein splicing. Proc Natl Acad Sci U S A 101(29): 10505-10. PubMed ID: 15247421
124.  (INT) Butler, M. I.; Goodwin, T. J.; Poulter, R. T. (2001) A nuclear-encoded intein in the fungal pathogen Cryptococcus neoformans. Yeast 18(15): 1365-70. PubMed ID: 11746598
125.  (EN) (INT) Butler, M. I.; Poulter, R. T. (2005) The PRP8 inteins in Cryptococcus are a source of phylogenetic and epidemiological information. Fungal Genet Biol 42(5): 452-63. PubMed ID: 15809009
126.  (INT) Butler, M. I.; Goodwin, T. J.; Poulter, R. T. (2005B) Two new fungal inteins. Yeast 22(6): 493-501. PubMed ID: 15849795
127.  (EN) (INT) Butler, M. I.; Gray, J.; Goodwin, T. J.; Poulter, R. T. (2006) The distribution and evolutionary history of the PRP8 intein. BMC Evol Biol. 6(1): 42. PubMed ID: 16737526
128.  (APP) (REL) Camarero, J. A.; Muir, T. W. (1997) Chemoselective backbone cyclization of unprotected peptides. Chem Commun 1997: 1369-1370.
129.  (APP) (CYCL) Camarero, J. A.; Pavel, J.; Muir, T. W. (1998) Chemical Synthesis of a Circular Protein Domain: Evidence for Folding-Assisted Cyclization. Angew. Chem. Int. Ed. 37: 347-349.
130.  (APP) (REL) (REV) Camarero, J.A. ; Muir, T.W. (1999) Native Chemical Ligation of Polypeptides. Current Protocols in Protein Science 18.4.1-18.4.21.
131.  (APP) (CYCL) Camarero, J. A.; Muir, T. W. (1999B) Biosynthesis of a Head-to Tail Cyclized Protein with Improved Biological Activity. J. Amer. Chem. Soc. 121: 5597-5598.
132.  (APP) (REL) Camarero, J. A.; Fushman, D.; Sato, S.; Giriat, I.; Cowburn, D.; Raleigh, D. P.; Muir, T. W. (2001) Rescuing a destabilized protein fold through backbone cyclization. J Mol Biol 308(5): 1045-62. PubMed ID: 11352590
133.  (APP) (CYCL) Camarero, J. A.; Fushman, D.; Cowburn, D.; Muir, T. W. (2001B) Peptide chemical ligation inside living cells: in vivo generation of a circular protein domain. Bioorg Med Chem 9(9): 2479-84. PubMed ID: 11553489
134.  (APP) (EPL) (NMR) (PUR) (Structure) Camarero, J. A.; Shekhtman, A.; Campbell, E. A.; Chlenov, M.; Gruber, T. M.; Bryant, D. A.; Darst, S. A.; Cowburn, D.; Muir, T. W. (2002) Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR. Proc Natl Acad Sci U S A 99(13): 8536-41. PubMed ID: 12084914
135.  (EPL) (REL) Camarero, J. A.; Hackel, B. J.; de Yoreo, J. J.; Mitchell, A. R. (2004) Fmoc-based synthesis of peptide alpha-thioesters using an aryl hydrazine support. J Org Chem 69(12): 4145-51. PubMed ID: 15176841
136.  (APP) (EPL) (TAG) Camarero, J. A.; Kwon, Y.; Coleman, M. A. (2004B) Chemoselective attachment of biologically active proteins to surfaces by expressed protein ligation and its application for "protein chip" fabrication. J Am Chem Soc 126(45): 14730-1. PubMed ID: 15535692
137.  (APP) (EPL) (REL) (REV) Camarero, J. A.; Mitchell, A. R. (2005) Synthesis of proteins by native chemical ligation using Fmoc-based chemistry. Protein Pept Lett 12(8): 723-8. PubMed ID: 16305540
138.  (APP) (CYCL) (PUR) Camarero, J. A.; Kimura, R. H.; Woo, Y. H.; Shekhtman, A.; Cantor, J. (2007) Biosynthesis of a fully functional cyclotide inside living bacterial cells. Chembiochem 8(12): 1363-1366.
139.  (APP) (EPL) (REV) (TAG) Camarero, J. A. (2008) Recent developments in the site-specific immobilization of proteins onto solid supports. Biopolymers 90(3): 450-8. PubMed ID: 17618518
140.  (APP) (REV) (TAG) (TS) Camarero, J. A.; Kwon, Y. (2008B) Traceless and Site-specific Attachment of Proteins onto Solid Supports. International Journal of Peptide Research and Therapeutics 14: 351-357.
141.  (APP) (EPL) (REV) (TS) Camarero, Julio A. (2009) Optimizing the future for biotechnology therapies, the key role of protein engineering. Advanced Drug Delivery Reviews 61(11): 897-898.
142.  (INT) Cambon-Bonavita, M. A.; Schmitt, P.; Zieger, M.; Flaman, J. M.; Lesongeur, F.; Raguenes, G.; Bindel, D.; Frisch, N.; Lakkis, Z.; Dupret, D.; Barbier, G.; Querellou, J. (2000) Cloning, expression, and characterization of DNA polymerase I from the hyperthermophilic archaea Thermococcus fumicolans. Extremophiles 4(4): 215-25. PubMed ID: 10972190
143.  (APP) (CPS) (NEB) Cann, I. K.; Amaya, K. R.; Southworth, M. W.; Perler, F. B. (2004) Bacteriophage-based genetic system for selection of nonsplicing inteins. Appl Environ Microbiol 70(5): 3158-62. PubMed ID: 15128583
144.  (APP) (NEB) (PUR) Cantor, E. J.; Chong, S. (2001) Intein-mediated rapid purification of Cre recombinase. Protein Expr Purif 22(1): 135-40. PubMed ID: 11388811
145.  (REL) Capasso, S.; Mazzarella, L.; Sorrentino, G.; Balboni, G.; Kirby, A. J. (1996) Kinetics and mechanism of the cleavage of the peptide bond next to asparagine. Peptides 17(6): 1075-7. PubMed ID: 8899829
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148.  (APP) (EPL) (REL) (REV) Casi, Giulio; Hilvert, Donald (2003) Convergent protein synthesis. Current Opinion in Structural Biology 13(5): 589-594.
149.  (INT) Caspi, J.; Amitai, G.; Belenkiy, O.; Pietrokovski, S. (2003) Distribution of split DnaE inteins in cyanobacteria. Mol Microbiol 50(5): 1569-77. PubMed ID: 14651639
150.  (APP) (PUR) Cattoli, Francesca; Sarti, Giulio C. (2002) Characterization of amylose affinity membranes through adsorption of pure recombinant MBP-intein-CBD: equilibrium and kinetics data. Desalination 149(1-3): 465-470.
151.  (APP) (PUR) Cattoli, Francesca; Boi, Cristiana; Sorci, Mirco; Sarti, Giulio C. (2006) Adsorption of pure recombinant MBP-fusion proteins on amylose affinity membranes. Journal of Membrane Science 273(1-2): 2-11.
152.  (APP) (EPL) (TAG) Ceccarini, G.; Flavell, R. R.; Butelman, E. R.; Synan, M.; Willnow, T. E.; Bar-Dagan, M.; Goldsmith, S. J.; Kreek, M. J.; Kothari, P.; Vallabhajosula, S.; Muir, T. W.; Friedman, J. M. (2009) PET imaging of leptin biodistribution and metabolism in rodents and primates. Cell Metab 10(2): 148-59. PubMed ID: 19656493
153.  (APP) (PUR) Celis, R. T. (1999) Repression and activation of arginine transport genes in Escherichia coli K 12 by the ArgP protein. J Mol Biol 294(5): 1087-95. PubMed ID: 10600368
154.  (APP) (EPL) (Structure) Chacko, B. M.; Qin, B. Y.; Tiwari, A.; Shi, G.; Lam, S.; Hayward, L. J.; De Caestecker, M.; Lin, K. (2004) Structural basis of heteromeric smad protein assembly in TGF-beta signaling. Mol Cell 15(5): 813-23. PubMed ID: 15350224
155.  (APP) (EPL) (TAG) Chaisemartin, L.; Chinestra, P.; Favre, G.; Blonski, C.; Faye, J. C. (2010) Synthesis and Application of a N-1' Fluorescent Biotinyl Derivative Inducing the Specific Carboxy-Terminal Dual Labeling of a Novel RhoB-Selective scFv. Bioconjug Chem 20(5): 847-855. PubMed ID: 19348471
156.  (APP) (REV) (TAG) Chamberlain, C.; Hahn, K. M. (2000) Watching proteins in the wild: fluorescence methods to study protein dynamics in living cells. Traffic 1(10): 755-62. PubMed ID: 11208065
157.  (APP) (EPL) (NEB) Chan, S. H.; Zhu, Z.; Dunigan, D. D.; Van Etten, J. L.; Xu, S. Y. (2006) Cloning of Nt.CviQII nicking endonuclease and its cognate methyltransferase: M.CviQII methylates AG sequences. Protein Expr Purif 49(1): 138-50. PubMed ID: 16737828
158.  (APP) (EPL) Chang, M. C.; Yee, C. S.; Nocera, D. G.; Stubbe, J. (2004) Site-specific replacement of a conserved tyrosine in ribonucleotide reductase with an aniline amino acid: a mechanistic probe for a redox-active tyrosine. J Am Chem Soc 126(51): 16702-3. PubMed ID: 15612690
159.  (APP) (TAG) (TS) Charalambous, A.; Andreou, M.; Skourides, P. A. (2009) Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo. J Nanobiotechnology 7: 9. PubMed ID: 20003264
160.  (APP) (EPL) (TAG) Chatterjee, C.; McGinty, R. K.; Pellois, J. P.; Muir, T. W. (2007) Auxiliary-mediated site-specific peptide ubiquitylation. Angew Chem Int Ed Engl 46(16): 2814-8. PubMed ID: 17366504
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162.  (APP) (EPL) Chatterjee, C.; Muir, T. W. (2010B) Chemical approaches for studying histone modifications. J Biol Chem 285(15): 11045-50. PubMed ID: 20147749
163.  (APP) (EPL) (PUR) (REV) Chattopadhaya, S.; Tan, L. P.; Yao, S. Q. (2006) Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays. Nat Protoc 1(5): 2386-98. PubMed ID: 17406482
164.  (APP) (EPL) (PUR) (REV) Chattopadhaya, S.; Srinivasan, R.; Yeo, D. S.; Chen, G. Y.; Yao, S. Q. (2009) Site-specific covalent labeling of proteins inside live cells using small molecule probes. Bioorg Med Chem 17(3): 981-9. PubMed ID: 18261914
165.  (APP) (EPL) (REV) (TAG) Chattopadhaya, S.; Abu Bakar, F. B.; Yao, S. Q. (2009B) Use of intein-mediated protein ligation strategies for the fabrication of functional protein arrays. Methods Enzymol 462: 195-223. PubMed ID: 19632476
166.  (APP) (PUR) Che, N.; Wang, L.; Gao, Y.; An, C. (2009) Soluble expression and one-step purification of a neurotoxin Huwentoxin-I in Escherichia coli. Protein Expr Purif 65(2): 154-9. PubMed ID: 19217942
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168.  (APP) (NEB) (TS) Chen, L.; Pradhan, S.; Evans, T. C., Jr. (2001) Herbicide resistance from a divided EPSPS protein: the split Synechocystis DnaE intein as an in vivo affinity domain. Gene 263(1-2): 39-48. PubMed ID: 11223241
169.  (INT) (NEB) (Structure) Chen, X.; Xu, M. Q.; Ding, Y.; Ferrandon, S.; Rao, Z. (2002) Purification and initial crystallization studies of a DnaB intein from Synechocystis sp. PCC 6803. Acta Crystallogr D Biol Crystallogr 58(Pt 7): 1201-3. PubMed ID: 12077443
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171.  (APP) (NEB) (PUR) Chen, L. Y.; Yi, X. Y.; Zeng, X. F.; Zhang, S. K.; McReynolds, L. (2004) [Mucosal immunization of recombinant Schistosoma japonicum ferritin]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 22(3): 129-32. PubMed ID: 15597704
172.  (APP) (PUR) Chen, H. W.; North, S. H.; Nakai, H. (2004) Properties of the PriA helicase domain and its role in binding PriA to specific DNA structures. J Biol Chem 279(37): 38503-12. PubMed ID: 15252043
173.  (APP) (NEB) (PUR) Chen, L. Y.; Yi, X. Y.; Zeng, X. F.; Cai, C.; McReynolds, L. (2005) A new antigen gene of Schistosoma japonicum: Screening, cloning, expression and immunization in mice. Progress in Biochemistry and Biophysics 32(4): 331-337.
174.  (APP) (PUR) (TS) Chen, L.; Zhu, F.; Li, J.; Lu, H.; Jiang, H.; Sarkar, R.; Arruda, V. R.; Wang, J.; Zhao, J.; Pierce, G. F.; Ding, Q.; Wang, X.; Wang, H.; Pipe, S. W.; Liu, X. Q.; Xiao, X.; Camire, R. M.; Xiao, W. (2007) The enhancing effects of the light chain on heavy chain secretion in split delivery of factor VIII gene. Mol Ther 15(10): 1856-62. PubMed ID: 17653101
175.  (APP) (PUR) Chen, Y. Q.; Zhang, S. Q.; Li, B. C.; Qiu, W.; Jiao, B.; Zhang, J.; Diao, Z. Y. (2008) Expression of a cytotoxic cationic antibacterial peptide in Escherichia coli using two fusion partners. Protein Expr Purif 57(2): 303-11. PubMed ID: 17977015
176.  (APP) (PUR) Chen, Y. Q.; Qiu, W.; Jiao, B.; Zhang, J.; Zhang, S. Q. (2008) Fusion expression of antibacterial and antitumorial peptide CM4N in Escherichia coli and its activity identification. Pharmaceutical Biotechnology 15(3): 161-165.
177.  (INT) Chen, C. L.; Pan, T. Y.; Kan, S. C.; Kuan, Y. C.; Hong, L. Y.; Chiu, K. R.; Sheu, C. S.; Yang, J. S.; Hsu, W. H.; Hu, H. Y. (2008B) Genome sequence of the lytic bacteriophage P1201 from Corynebacterium glutamicum NCHU 87078: evolutionary relationships to phages from Corynebacterineae. Virology 378(2): 226-32. PubMed ID: 18599103
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180.  (APP) (CYCL) (TAG) Cheng, L.; Naumann, T. A.; Horswill, A. R.; Hong, S. J.; Venters, B. J.; Tomsho, J. W.; Benkovic, S. J.; Keiler, K. C. (2007) Discovery of antibacterial cyclic peptides that inhibit the ClpXP protease. Protein Sci 16(8): 1535-42. PubMed ID: 17600141
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190.  (APP) (NEB) (TS) Chin, H. G.; Kim, G. D.; Marin, I.; Mersha, F.; Evans, T. C., Jr.; Chen, L.; Xu, M. Q.; Pradhan, S. (2003) Protein trans-splicing in transgenic plant chloroplast: reconstruction of herbicide resistance from split genes. Proc Natl Acad Sci U S A 100(8): 4510-5. PubMed ID: 12671070
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205.  (APP) (PUR) Christensen, S. K.; Mikkelsen, M.; Pedersen, K.; Gerdes, K. (2001) RelE, a global inhibitor of translation, is activated during nutritional stress. Proc Natl Acad Sci U S A 98(25): 14328-33. PubMed ID: 11717402
206.  (APP) (PUR) (TAG) (TS) Chu, N. K.; Becker, C. F. (2009) Semisynthesis of membrane-attached prion proteins. Methods Enzymol 462: 177-93. PubMed ID: 19632475
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233.  (APP) (EPL) (NMR) (REV) (Structure) Cowburn, D.; Muir, T. W. (2001) Segmental isotopic labeling using expressed protein ligation. Methods Enzymol 339: 41-54. PubMed ID: 11462824
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373.  (APP) (PUR) Gaitatzis, N.; Kunze, B.; Muller, R. (2001) In vitro reconstitution of the myxochelin biosynthetic machinery of Stigmatella aurantiaca Sg a15: Biochemical characterization of a reductive release mechanism from nonribosomal peptide synthetases. Proc Natl Acad Sci U S A 98(20): 11136-41. PubMed ID: 11562468
374.  (APP) (PUR) Gan-Erdene, T.; Nagamalleswari, K.; Yin, L.; Wu, K.; Pan, Z. Q.; Wilkinson, K. D. (2003) Identification and characterization of DEN1, a deneddylase of the ULP family. J Biol Chem 278(31): 28892-900. PubMed ID: 12759362
375.  (APP) (PUR) Gan, X.; Ma, Z.; Deng, N.; Wang, J.; Ding, J.; Li, L. (2004) Involvement of the C-terminal proline-rich motif of G protein-coupled receptor kinases in recognition of activated rhodopsin. J Biol Chem 279(48): 49741-6. PubMed ID: 15375171
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377.  (APP) (PUR) Gangopadhyay, J. P.; Jiang, S. Q.; van Berkel, P.; Paulus, H. (2003) In vitro splicing of erythropoietin by the Mycobacterium tuberculosis RecA intein without substituting amino acids at the splice junctions. Biochim Biophys Acta 1619(2): 193-200. PubMed ID: 12527116
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467.  (APP) Hassiepen, U.; Eidhoff, U.; Meder, G.; Bulber, J. F.; Hein, A.; Bodendorf, U.; Lorthiois, E.; Martoglio, B. (2007) A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine110-glycine as substrate. Anal Biochem 371(2): 201-7. PubMed ID: 17869210
468.  (INT) Hatfull, G. F.; Pedulla, M. L.; Jacobs-Sera, D.; Cichon, P. M.; Foley, A.; Ford, M. E.; Gonda, R. M.; Houtz, J. M.; Hryckowian, A. J.; Kelchner, V. A.; Namburi, S.; Pajcini, K. V.; Popovich, M. G.; Schleicher, D. T.; Simanek, B. Z.; Smith, A. L.; Zdanowicz, G. M.; Kumar, V.; Peebles, C. L.; Jacobs, W. R., Jr.; Lawrence, J. G.; Hendrix, R. W. (2006) Exploring the mycobacteriophage metaproteome: phage genomics as an educational platform. PLoS Genet 2(6): e92. PubMed ID: 16789831
469.  (EN) Haugen, P.; Wikmark, O. G.; Vader, A.; Coucheron, D. H.; Sjottem, E.; Johansen, S. D. (2005) The recent transfer of a homing endonuclease gene. Nucleic Acids Res 33(8): 2734-41. PubMed ID: 15891115
470.  (APP) (EPL) (PUR) Hauser, P. S.; Ryan, R. O. (2007) Expressed protein ligation using an N-terminal cysteine containing fragment generated in vivo from a pelB fusion protein. Protein Expr Purif 54(2): 227-33. PubMed ID: 17493830
471.  (APP) (EPL) (NMR) (Structure) Hauser, P. S.; Raussens, V.; Yamamoto, T.; Abdullahi, G. E.; Weers, P. M.; Sykes, B. D.; Ryan, R. O. (2009) Semisynthesis and segmental isotope labeling of the apoE3 N-terminal domain using expressed protein ligation. J Lipid Res 50(8): 1548-55. PubMed ID: 19098282
472.  (EN) (INT) He, Z.; Crist, M.; Yen, H.; Duan, X.; Quiocho, F. A.; Gimble, F. S. (1998) Amino acid residues in both the protein splicing and endonuclease domains of the PI-SceI intein mediate DNA binding. J Biol Chem 273(8): 4607-15. PubMed ID: 9468518
473.  (EN) (Structure) Heath, P. J.; Stephens, K. M.; Monnat, R. J., Jr.; Stoddard, B. L. (1997) The structure of I-Crel, a group I intron-encoded homing endonuclease. Nat Struct Biol 4(6): 468-76. PubMed ID: 9187655
474.  (INT) (NMR) (Structure) Heinamaki, K.; Oeemig, J. S.; Djupsjobacka, J.; Iwai, H. (2009) NMR resonance assignment of DnaE intein from Nostoc punctiforme. Biomol NMR Assign 3(1): 41-3. PubMed ID: 19636943
475.  (APP) (PUR) Hemelaar, J.; Lelyveld, V. S.; Kessler, B. M.; Ploegh, H. L. (2003) A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L. J Biol Chem 278(51): 51841-50. PubMed ID: 14530254
476.  (APP) (EPL) (TAG) Hemelaar, J.; Borodovsky, A.; Kessler, B. M.; Reverter, D.; Cook, J.; Kolli, N.; Gan-Erdene, T.; Wilkinson, K. D.; Gill, G.; Lima, C. D.; Ploegh, H. L.; Ovaa, H. (2004) Specific and covalent targeting of conjugating and deconjugating enzymes of ubiquitin-like proteins. Mol Cell Biol 24(1): 84-95. PubMed ID: 14673145
477.  (APP) (EPL) (REL) (REV) Hemelaar, J.; Galardy, P. J.; Borodovsky, A.; Kessler, B. M.; Ploegh, H. L.; Ovaa, H. (2004) Chemistry-Based Functional Proteomics: Mechanism-Based Activity-Profiling Tools for Ubiquitin and Ubiquitin-like Specific Proteases. Journal of Proteome Research 3(2): 268-276.
478.  (INT) Hendrickson, E. L.; Kaul, R.; Zhou, Y.; Bovee, D.; Chapman, P.; Chung, J.; Conway de Macario, E.; Dodsworth, J. A.; Gillett, W.; Graham, D. E.; Hackett, M.; Haydock, A. K.; Kang, A.; Land, M. L.; Levy, R.; Lie, T. J.; Major, T. A.; Moore, B. C.; Porat, I.; Palmeiri, A.; Rouse, G.; Saenphimmachak, C.; Soll, D.; Van Dien, S.; Wang, T.; Whitman, W. B.; Xia, Q.; Zhang, Y.; Larimer, F. W.; Olson, M. V.; Leigh, J. A. (2004) Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis. J Bacteriol 186(20): 6956-69. PubMed ID: 15466049
479.  (REL) (REV) Hendrix, R. W. (1991) Protein carpentry. Curr Biol. 1(2): 71-3.. PubMed ID: 15336168
480.  (INT) Henne, A.; Bruggemann, H.; Raasch, C.; Wiezer, A.; Hartsch, T.; Liesegang, H.; Johann, A.; Lienard, T.; Gohl, O.; Martinez-Arias, R.; Jacobi, C.; Starkuviene, V.; Schlenczeck, S.; Dencker, S.; Huber, R.; Klenk, H. P.; Kramer, W.; Merkl, R.; Gottschalk, G.; Fritz, H. J. (2004) The genome sequence of the extreme thermophile Thermus thermophilus. Nat Biotechnol. 22(5): 547-53. Epub 2004 Apr 4.. PubMed ID: 15064768
481.  (INT) Henneke, G.; Gueguen, Y.; Flament, D.; Azam, P.; Querellou, J.; Dietrich, J.; Hubscher, U.; Raffin, J. P. (2002) Replication factor C from the hyperthermophilic archaeon Pyrococcus abyssi does not need ATP hydrolysis for clamp-loading and contains a functionally conserved RFC PCNA-binding domain. J Mol Biol 323(5): 795-810. PubMed ID: 12417194
482.  (APP) (PUR) Herrmann, R.; Heck, M.; Henklein, P.; Kleuss, C.; Hofmann, K. P.; Ernst, O. P. (2004) Sequence of interactions in receptor-G protein coupling. J Biol Chem 279(23): 24283-90. PubMed ID: 15007073
483.  (INT) Hertveldt, K.; Lavigne, R.; Pleteneva, E.; Sernova, N.; Kurochkina, L.; Korchevskii, R.; Robben, J.; Mesyanzhinov, V.; Krylov, V. N.; Volckaert, G. (2005) Genome comparison of Pseudomonas aeruginosa large phages. J Mol Biol 354(3): 536-45. PubMed ID: 16256135
484.  (INT) (REV) Hickey, D. A. (1994) Protein introns: optional or essential?. Trends Genet. 10(5): 147-9.. PubMed ID: 8036715
485.  (EN) (INT) Hiraga, K.; Derbyshire, V.; Dansereau, J. T.; Van Roey, P.; Belfort, M. (2005) Minimization and stabilization of the Mycobacterium tuberculosis recA intein. J Mol Biol 354(4): 916-26. PubMed ID: 16288917
486.  (INT) Hiraga, K.; Soga, I.; Dansereau, J. T.; Pereira, B.; Derbyshire, V.; Du, Z.; Wang, C.; Van Roey, P.; Belfort, G.; Belfort, M. (2009) Selection and structure of hyperactive inteins: peripheral changes relayed to the catalytic center. J Mol Biol 393(5): 1106-17. PubMed ID: 19744499
487.  (INT) Hirata, R.; Ohsumk, Y.; Nakano, A.; Kawasaki, H.; Suzuki, K.; Anraku, Y. (1990) Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. J Biol Chem. 265(12): 6726-33.. PubMed ID: 2139027
488.  (INT) Hirata, R.; Anraku, Y. (1992) Mutations at the putative junction sites of the yeast VMA1 protein, the catalytic subunit of the vacuolar membrane H(+)-ATPase, inhibit its processing by protein splicing. Biochem Biophys Res Commun 188(1): 40-7. PubMed ID: 1417861
489.  (APP) (PUR) Hoang, T. T.; Ma, Y.; Stern, R. J.; McNeil, M. R.; Schweizer, H. P. (1999) Construction and use of low-copy number T7 expression vectors for purification of problem proteins: purification of mycobacterium tuberculosis RmlD and pseudomonas aeruginosa LasI and RhlI proteins, and functional analysis of purified RhlI. Gene 237(2): 361-71. PubMed ID: 10521660
490.  (EN) (INT) (NEB) Hodges, R. A.; Perler, F. B.; Noren, C. J.; Jack, W. E. (1992) Protein splicing removes intervening sequences in an archaea DNA polymerase. Nucleic Acids Res 20(23): 6153-7. PubMed ID: 1475179
491.  (APP) (EPL) (REV) Hodgson, D. R. W.; Sanderson, J. M. (2004) The synthesis of peptides and proteins containing non-natural amino acids. Chemical Society Reviews 33(7): 422-430.
492.  (APP) (EPL) (TAG) Hofmann, R. M.; Cotton, G. J.; Chang, E. J.; Vidal, E.; Veach, D.; Bornmann, W.; Muir, T. W. (2001) Fluorescent monitoring of kinase activity in real time: development of a robust fluorescence-based assay for Abl tyrosine kinase activity. Bioorg Med Chem Lett. 11(24): 3091-4.. PubMed ID: 11720849
493.  (APP) (EPL) (REV) Hofmann, R. M.; Muir, T. W. (2002) Recent advances in the application of expressed protein ligation to protein engineering. Curr Opin Biotechnol 13(4): 297-303. PubMed ID: 12323349
494.  (APP) (PUR) (Structure) (TS) Hofmann, A.; Iwai, H.; Hess, S.; Pluckthun, A.; Wlodawer, A. (2002B) Structure of cyclized green fluorescent protein. Acta Crystallogr D Biol Crystallogr 58(Pt 9): 1400-6. PubMed ID: 12198295
495.  (REV) (TS) Holden, M.; Sebaihia, M.; Cerdeno-Tarraga, A.; Parkhill, J. (2002) Split personalities. Trends Microbiol. 10(3): 115.. PubMed ID: 11864813
496.  (APP) (EPL) (PUR) (TAG) Holder, J. W.; Craig, N. L. (2010) Architecture of the Tn7 posttransposition complex: an elaborate nucleoprotein structure. J Mol Biol 401(2): 167-81. PubMed ID: 20538004
497.  (APP) (REV) Holford, M.; Muir, T. W. (1998) Adding 'splice' to protein engineering. Structure 6(8): 951-6. PubMed ID: 9739093
498.  (APP) (EPL) (TAG) Holland-Nell, K.; Beck-Sickinger, A. G. (2007) Specifically Immobilised Aldo/Keto Reductase AKR1A1 Shows a Dramatic Increase in Activity Relative to the Randomly Immobilised Enzyme. Chembiochem 8(9): 1071-1076. PubMed ID: 17508367
499.  (APP) (EPL) (TAG) Hondal, R. J.; Nilsson, B. L.; Raines, R. T. (2001) Selenocysteine in native chemical ligation and expressed protein ligation. J Am Chem Soc 123(21): 5140-1. PubMed ID: 11457362
500.  (APP) (EPL) (TAG) Hondal, R. J. (2005) Incorporation of selenocysteine into proteins using peptide ligation. Protein Pept Lett 12(8): 757-64. PubMed ID: 16305545
501.  (APP) (EPL) (REV) Hondal, R. J. (2009) Using chemical approaches to study selenoproteins-Focus on thioredoxin reductases. Biochim Biophys Acta 1790(11): 1501-12. PubMed ID: 19406205
502.  (APP) (PUR) Hong, S.; Toyama, M.; Maret, W.; Murooka, Y. (2001) High yield expression and single step purification of human thionein/metallothionein. Protein Expr Purif 21(1): 243-50. PubMed ID: 11162412
503.  (APP) (EPL) (TAG) Hong, S. H.; Maret, W. (2003) A fluorescence resonance energy transfer sensor for the beta-domain of metallothionein. Proc Natl Acad Sci U S A. 100(5): 2255-60.. PubMed ID: 12618543
504.  (APP) (PUR) Hong, J.; Wang, Y.; Ye, X.; Zhang, Y. H. (2008) Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage. J Chromatogr A 1194(2): 150-154. PubMed ID: 18468611
505.  (APP) (PUR) Hong, I.; Kim, Y. S.; Choi, S. G. (2010) Simple purification of human antimicrobial peptide dermcidin (MDCD-1L) by intein-mediated expression in E.coli. J Microbiol Biotechnol 20(2): 350-5. PubMed ID: 20208440
506.  (APP) (EPL) (TAG) Hori, Y.; Egashira, Y.; Kamiura, R.; Kikuchi, K. (2010) Noncovalent-interaction-promoted ligation for protein labeling. Chembiochem 11(5): 646-648.
507.  (APP) (CYCL) (TS) Horswill, A. R.; Savinov, S. N.; Benkovic, S. J. (2004) A systematic method for identifying small-molecule modulators of protein-protein interactions. Proc Natl Acad Sci U S A 101(44): 15591-6. PubMed ID: 15498867
508.  (APP) (CYCL) (REV) Horswill, A. R.; Benkovic, S. J. (2005) Cyclic peptides, a chemical genetics tool for biologists. Cell Cycle 4(4): 552-5. PubMed ID: 15876867
509.  (APP) (CYCL) (REV) Horswill, A. R.; Benkovic, S. J. (2006) Identifying small-molecule modulators of protein-protein interactions. Curr Protoc Protein Sci Chapter 19: Unit 19 15. PubMed ID: 18429304
510.  (EN) (INT) Hu, D.; Crist, M.; Duan, X.; Gimble, F. S. (1999) Mapping of a DNA binding region of the PI-sceI homing endonuclease by affinity cleavage and alanine-scanning mutagenesis. Biochemistry 38(39): 12621-8. PubMed ID: 10504231
511.  (EN) (INT) Hu, D.; Crist, M.; Duan, X.; Quiocho, F. A.; Gimble, F. S. (2000) Probing the structure of the PI-SceI-DNA complex by affinity cleavage and affinity photocross-linking. J Biol Chem 275(4): 2705-12. PubMed ID: 10644733
512.  (INT) Huang, C.; Wang, S.; Chen, L.; Lemieux, C.; Otis, C.; Turmel, M.; Liu, X. Q. (1994) The Chlamydomonas chloroplast clpP gene contains translated large insertion sequences and is essential for cell growth. Mol Gen Genet. 244(2): 151-9.. PubMed ID: 8052234
513.  (INT) Huet, G.; Daffe, M.; Saves, I. (2005) Identification of the Mycobacterium tuberculosis SUF machinery as the exclusive mycobacterial system of [Fe-S] cluster assembly: evidence for its implication in the pathogen's survival. J Bacteriol 187(17): 6137-46. PubMed ID: 16109955
514.  (INT) Huet, G.; Castaing, J. P.; Fournier, D.; Daffe, M.; Saves, I. (2006) Protein Splicing of SufB Is Crucial for the Functionality of the Mycobacterium tuberculosis SUF Machinery. J Bacteriol. 188(9): 3412-4. PubMed ID: 16621837
515.  (APP) (PUR) Humphries, H. E.; Christodoulides, M.; Heckels, J. E. (2002) Expression of the class 1 outer-membrane protein of Neisseria meningitidis in Escherichia coli and purification using a self-cleavable affinity tag. Protein Expr Purif 26(2): 243-8. PubMed ID: 12406678
516.  (APP) (PUR) Humphries, H. E.; Williams, J. N.; Christodoulides, M.; Heckels, J. E. (2004) Recombinant meningococcal PorA protein, expressed using a vector system with potential for human vaccination, induces a bactericidal immune response. Vaccine 22(11-12): 1564-9. PubMed ID: 15063582
517.  (APP) (PUR) (REV) Hunt, I. (2005) From gene to protein: a review of new and enabling technologies for multi-parallel protein expression. Protein Expr Purif 40(1): 1-22. PubMed ID: 15721767
518.  (APP) (REL) Hunter, C. L.; Kochendoerfer, G. G. (2004) Native chemical ligation of hydrophobic [corrected] peptides in lipid bilayer systems. Bioconjug Chem. 15(3): 437-40.. PubMed ID: 15149169
519.  (APP) (EPL) Huse, M.; Holford, M.N.; Kuriyan, J.; Muir, T.W. (2000) Semisynthesis of Hyperphosphorylated Type I Tgf beta Receptor: Addressing the Mechanism of Kinase Activation. J Amer Chem Soc 122(34): 8337-8338.
520.  (APP) (EPL) Huse, M.; Muir, T. W.; Xu, L.; Chen, Y. G.; Kuriyan, J.; Massague, J. (2001) The TGF beta receptor activation process: an inhibitor- to substrate-binding switch. Mol Cell. 8(3): 671-82.. PubMed ID: 11583628
521.  (INT) Iacono, M.; Villa, L.; Fortini, D.; Bordoni, R.; Imperi, F.; Bonnal, R. J.; Sicheritz-Ponten, T.; De Bellis, G.; Visca, P.; Cassone, A.; Carattoli, A. (2008) Whole-genome pyrosequencing of an epidemic multidrug-resistant Acinetobacter baumannii strain belonging to the European clone II group. Antimicrob Agents Chemother 52(7): 2616-25. PubMed ID: 18411315
522.  (APP) (EPL) (PUR) (REL) Iakovenko, A.; Rostkova, E.; Merzlyak, E.; Hillebrand, A. M.; Thoma, N. H.; Goody, R. S.; Alexandrov, K. (2000) Semi-synthetic Rab proteins as tools for studying intermolecular interactions. FEBS Lett. 468(2-3): 155-8.. PubMed ID: 10692577
523.  (EN) (INT) (Structure) Ichiyanagi, K.; Ishino, Y.; Ariyoshi, M.; Komori, K.; Morikawa, K. (2000) Crystal structure of an archaeal intein-encoded homing endonuclease PI-PfuI. J Mol Biol 300(4): 889-901. PubMed ID: 10891276
524.  (INT) Ikeda, H.; Ishikawa, J.; Hanamoto, A.; Shinose, M.; Kikuchi, H.; Shiba, T.; Sakaki, Y.; Hattori, M.; Omura, S. (2003) Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis. Nat Biotechnol. 21(5): 526-31. Epub 2003 Apr 14.. PubMed ID: 12692562
525.  (APP) (PUR) (REL) (Structure) Ikegami, T.; Okada, T.; Hashimoto, M.; Seino, S.; Watanabe, T.; Shirakawa, M. (2000) Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1. J Biol Chem 275(18): 13654-61. PubMed ID: 10788483
526.  (APP) (REL) (TAG) Inard, C.; Fourcade, E.; Baron, R.; Tovar, D.; Chaisemartin, L.; Blonski, C.; Faye, J. C. (2006) Syntheses of functionalized biotin N-1' derivatives: new tools for the control of gene expression with small molecules. Bioconjug Chem 17(4): 1030-5. PubMed ID: 16848412
527.  (APP) (PUR) Ingham, A. B.; Sproat, K. W.; Tizard, M. L.; Moore, R. J. (2005) A versatile system for the expression of nonmodified bacteriocins in Escherichia coli. J Appl Microbiol 98(3): 676-83. PubMed ID: 15715871
528.  (APP) (PUR) Inoue, K.; Demel, R.; de Kruijff, B.; Keegstra, K. (2001) The N-terminal portion of the preToc75 transit peptide interacts with membrane lipids and inhibits binding and import of precursor proteins into isolated chloroplasts. Eur J Biochem 268(14): 4036-43. PubMed ID: 11453998
529.  (INT) Ishikawa, J.; Yamashita, A.; Mikami, Y.; Hoshino, Y.; Kurita, H.; Hotta, K.; Shiba, T.; Hattori, M. (2004) The complete genomic sequence of Nocardia farcinica IFM 10152. Proc Natl Acad Sci U S A. 101(41): 14925-30. Epub 2004 Oct 4.. PubMed ID: 15466710
530.  (INT) Ivars-Martinez, E.; Martin-Cuadrado, A. B.; D'Auria, G.; Mira, A.; Ferriera, S.; Johnson, J.; Friedman, R.; Rodriguez-Valera, F. (2008) Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter. ISME J 2(12): 1194-212. PubMed ID: 18670397
531.  (REL) (REV) Iwai, K.; Ando, T. (1967) N-O acyl shifts. Meth. Enzymol. 11: 262-282.
532.  (APP) (CYCL) Iwai, H.; Pluckthun, A. (1999) Circular beta-lactamase: stability enhancement by cyclizing the backbone. FEBS Lett 459(2): 166-72. PubMed ID: 10518012
533.  (APP) (CYCL) Iwai, H.; Lingel, A.; Pluckthun, A. (2001) Cyclic green fluorescent protein produced in vivo using an artificially split PI-PfuI intein from Pyrococcus furiosus. J Biol Chem 276(19): 16548-54. PubMed ID: 11278952
534.  (INT) (TS) Iwai, H.; Zuger, S.; Jin, J.; Tam, P. H. (2006) Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett 580(7): 1853-1858. PubMed ID: 16516207
535.  (APP) (NMR) (REV) (Structure) Iwai, H.; Zuger, S. (2007) Protein ligation: applications in NMR studies of proteins. Biotechnol Genet Eng Rev 24: 129-45. PubMed ID: 18059630
536.  (INT) Iwai, H. (2010) Hideo Iwai. Interview by Daniela Ruffell. FEBS Lett 584(12): 2494-5. PubMed ID: 20450913
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538.  (REL) (REV) Jaffe, S. (2004) Humanizing Protein Splicing. The Scientist 18(11): 30-31.
539.  (INT) Jakob, N. J.; Muller, K.; Bahr, U.; Darai, G. (2001) Analysis of the first complete DNA sequence of an invertebrate iridovirus: coding strategy of the genome of Chilo iridescent virus. Virology 286(1): 182-96. PubMed ID: 11448171
540.  (APP) Jantz, D.; Berg, J. M. (2003) Expanding the DNA-recognition repertoire for zinc finger proteins beyond 20 amino acids. J Am Chem Soc. 125(17): 4960-1.. PubMed ID: 12708829
541.  (APP) (CYCL) Jeffries, C. M.; Graham, S. C.; Stokes, P. H.; Collyer, C. A.; Guss, J. M.; Matthews, J. M. (2006) Stabilization of a binary protein complex by intein-mediated cyclization. Protein Sci 15(11): 2612-8. PubMed ID: 17001033
542.  (INT) Jeffries, T. W.; Grigoriev, I. V.; Grimwood, J.; Laplaza, J. M.; Aerts, A.; Salamov, A.; Schmutz, J.; Lindquist, E.; Dehal, P.; Shapiro, H.; Jin, Y. S.; Passoth, V.; Richardson, P. M. (2007) Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis. Nat Biotechnol 25(3): 319-326. PubMed ID: 17334359
543.  (APP) (PUR) Jester, B. C.; Levengood, J. D.; Roy, H.; Ibba, M.; Devine, K. M. (2003) Nonorthologous replacement of lysyl-tRNA synthetase prevents addition of lysine analogues to the genetic code. Proc Natl Acad Sci U S A 100(24): 14351-6. PubMed ID: 14623972
544.  (APP) (PUR) Jgoun, A. A.; Eldarov, M. A.; Solodar, L. I.; Sokolov, N. N.; Archakov, A. I.; Skryabin, K. G. (2001) Heterologous expression of eukaryotic cytochromes P450 1. Heterologous expression of cytochrome P450 2B4 in E.coli as fusion proteins with different affiniti purification tags. Voprosy Meditsinskoj Khimii 47(4): 391-392.
545.  (REL) Johansson, D. G.; Macao, B.; Sandberg, A.; Hard, T. (2008) SEA domain autoproteolysis accelerated by conformational strain: mechanistic aspects. J Mol Biol 377(4): 1130-43. PubMed ID: 18314133
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548.  (INT) (NEB) (NMR) (Structure) Johnson, M. A.; Southworth, M. W.; Herrmann, T.; Brace, L.; Perler, F. B.; Wuthrich, K. (2007) NMR structure of a KlbA intein precursor from Methanococcus jannaschii. Protein Sci 16(7): 1316-28. PubMed ID: 17586768
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550.  (APP) (REV) Johnsson, N.; Johnsson, K. (2003) A fusion of disciplines: Chemical approaches to exploit fusion proteins for functional genomics. Chembiochem 4(9): 803-810.
551.  (INT) (REL) Jordan, A.; Torrents, E.; Jeanthon, C.; Eliasson, R.; Hellman, U.; Wernstedt, C.; Barbe, J.; Gibert, I.; Reichard, P. (1997) B12-dependent ribonucleotide reductases from deeply rooted eubacteria are structurally related to the aerobic enzyme from Escherichia coli. Proc Natl Acad Sci U S A. 94(25): 13487-92.. PubMed ID: 9391052
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554.  (APP) (INT) (REL) Kalia, J.; Raines, R. T. (2006) Reactivity of intein thioesters: appending a functional group to a protein. Chembiochem 7(9): 1375-83. PubMed ID: 16897799
555.  (APP) (EPL) (TAG) Kalia, J.; Abbott, N. L.; Raines, R. T. (2007) General method for site-specific protein immobilization by Staudinger ligation. Bioconjug Chem 18(4): 1064-9. PubMed ID: 17488064
556.  (APP) (EPL) (TAG) Kallenberger, B. C.; Love, J. D.; Chatterjee, V. K.; Schwabe, J. W. (2003) A dynamic mechanism of nuclear receptor activation and its perturbation in a human disease. Nat Struct Biol. 10(2): 136-40.. PubMed ID: 12536206
557.  (INT) Kane, P. M.; Yamashiro, C. T.; Wolczyk, D. F.; Neff, N.; Goebl, M.; Stevens, T. H. (1990) Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase. Science 250(4981): 651-7. PubMed ID: 2146742
558.  (INT) Kaneko, T.; Tanaka, A.; Sato, S.; Kotani, H.; Sazuka, T.; Miyajima, N.; Sugiura, M.; Tabata, S. (1995) Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome. DNA Res. 2(4): 153-66, 191-98.. PubMed ID: 8590279
559.  (INT) Kaneko, T.; Sato, S.; Kotani, H.; Tanaka, A.; Asamizu, E.; Nakamura, Y.; Miyajima, N.; Hirosawa, M.; Sugiura, M.; Sasamoto, S.; Kimura, T.; Hosouchi, T.; Matsuno, A.; Muraki, A.; Nakazaki, N.; Naruo, K.; Okumura, S.; Shimpo, S.; Takeuchi, C.; Wada, T.; Watanabe, A.; Yamada, M.; Yasuda, M.; Tabata, S. (1996) Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions. DNA Res. 3(3): 109-36.. PubMed ID: 8905231
560.  (INT) Kaneko, T.; Sato, S.; Kotani, H.; Tanaka, A.; Asamizu, E.; Nakamura, Y.; Miyajima, N.; Hirosawa, M.; Sugiura, M.; Sasamoto, S.; Kimura, T.; Hosouchi, T.; Matsuno, A.; Muraki, A.; Nakazaki, N.; Naruo, K.; Okumura, S.; Shimpo, S.; Takeuchi, C.; Wada, T.; Watanabe, A.; Yamada, M.; Yasuda, M.; Tabata, S. (1996B) Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions (supplement). DNA Res. 3(3): 185-209.. PubMed ID: 8905238
561.  (INT) Kaneko, T.; Tabata, S. (1997) Complete genome structure of the unicellular cyanobacterium Synechocystis sp. PCC6803. Plant Cell Physiol. 38(11): 1171-6.. PubMed ID: 9435137
562.  (INT) Kaneko, T.; Nakamura, Y.; Wolk, C. P.; Kuritz, T.; Sasamoto, S.; Watanabe, A.; Iriguchi, M.; Ishikawa, A.; Kawashima, K.; Kimura, T.; Kishida, Y.; Kohara, M.; Matsumoto, M.; Matsuno, A.; Muraki, A.; Nakazaki, N.; Shimpo, S.; Sugimoto, M.; Takazawa, M.; Yamada, M.; Yasuda, M.; Tabata, S. (2001) Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. DNA Res. 8(5): 205-13; 227-53.. PubMed ID: 11759840
563.  (INT) Kaneko, T.; Nakajima, N.; Okamoto, S.; Suzuki, I.; Tanabe, Y.; Tamaoki, M.; Nakamura, Y.; Kasai, F.; Watanabe, A.; Kawashima, K.; Kishida, Y.; Ono, A.; Shimizu, Y.; Takahashi, C.; Minami, C.; Fujishiro, T.; Kohara, M.; Katoh, M.; Nakazaki, N.; Nakayama, S.; Yamada, M.; Tabata, S.; Watanabe, M. M. (2007) Complete genomic structure of the bloom-forming toxic cyanobacterium Microcystis aeruginosa NIES-843. DNA Res 14(6): 247-56. PubMed ID: 18192279
564.  (APP) (TS) Kanno, A.; Ozawa, T.; Umezawa, Y. (2006) Intein-mediated reporter gene assay for detecting protein-protein interactions in living mammalian cells. Anal Chem 78(2): 556-60. PubMed ID: 16408939
565.  (APP) (CPS) (TAG) (TS) Kanno, A.; Ozawa, T.; Umezawa, Y. (2006B) Genetically encoded optical probe for detecting release of proteins from mitochondria toward cytosol in living cells and mammals. Anal Chem 78(23): 8076-81. PubMed ID: 17134142
566.  (APP) (CYCL) (TAG) Kanno, A.; Yamanaka, Y.; Hirano, H.; Umezawa, Y.; Ozawa, T. (2007) Cyclic luciferase for real-time sensing of caspase-3 activities in living mammals. Angew Chem Int Ed Engl 46(40): 7595-9. PubMed ID: 17722214
567.  (APP) (CPS) (TAG) (TS) Kanno, A.; Ozawa, T.; Umezawa, Y. (2009) Bioluminescent imaging of MAPK function with intein-mediated reporter gene assay. Methods Mol Biol 574: 185-92. PubMed ID: 19685309
568.  (APP) (EPL) Karow, A. R.; Theissen, B.; Klostermeier, D. (2007) Authentic interdomain communication in an RNA helicase reconstituted by expressed protein ligation of two helicase domains. Febs J 274(2): 463-73. PubMed ID: 17229151
569.  (APP) (PUR) Karring, H.; Bjornsson, A.; Thirup, S.; Clark, B. F.; Knudsen, C. R. (2003) Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts. Eur J Biochem 270(21): 4294-305. PubMed ID: 14622294
570.  (APP) (EPL) (TAG) Karukurichi, K. R.; Wang, L.; Uzasci, L.; Manlandro, C. M.; Wang, Q.; Cole, P. A. (2010) Analysis of p300/CBP histone acetyltransferase regulation using circular permutation and semisynthesis. J Am Chem Soc 132(4): 1222-3. PubMed ID: 20063892
571.  (APP) (EPL) Katayama, H.; Hojo, H.; Ohira, T.; Ishii, A.; Nozaki, T.; Goto, K.; Nakahara, Y.; Takahashi, T.; Hasegawa, Y.; Nagasawa, H. (2010) Correct disulfide pairing is required for the biological activity of crustacean androgenic gland hormone (AGH): synthetic studies of AGH. Biochemistry 49(8): 1798-807. PubMed ID: 20092253
572.  (APP) (EPL) (PUR) Katragadda, M.; Lambris, J. D. (2006) Expression of compstatin in Escherichia coli: Incorporation of unnatural amino acids enhances its activity. Protein Expr Purif 47(1): 289-95. PubMed ID: 16406678
573.  (INT) Kawarabayasi, Y.; Sawada, M.; Horikawa, H.; Haikawa, Y.; Hino, Y.; Yamamoto, S.; Sekine, M.; Baba, S.; Kosugi, H.; Hosoyama, A.; Nagai, Y.; Sakai, M.; Ogura, K.; Otsuka, R.; Nakazawa, H.; Takamiya, M.; Ohfuku, Y.; Funahashi, T.; Tanaka, T.; Kudoh, Y.; Yamazaki, J.; Kushida, N.; Oguchi, A.; Aoki, K.; Kikuchi, H. (1998) Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3. DNA Res 5(2): 55-76. PubMed ID: 9679194
574.  (INT) Kawarabayasi, Y.; Sawada, M.; Horikawa, H.; Haikawa, Y.; Hino, Y.; Yamamoto, S.; Sekine, M.; Baba, S.; Kosugi, H.; Hosoyama, A.; Nagai, Y.; Sakai, M.; Ogura, K.; Otsuka, R.; Nakazawa, H.; Takamiya, M.; Ohfuku, Y.; Funahashi, T.; Tanaka, T.; Kudoh, Y.; Yamazaki, J.; Kushida, N.; Oguchi, A.; Aoki, K.; Kikuchi, H. (1998B) Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3 (supplement). DNA Res. 5(2): 147-55.. PubMed ID: 9679203
575.  (INT) Kawarabayasi, Y.; Hino, Y.; Horikawa, H.; Yamazaki, S.; Haikawa, Y.; Jin-no, K.; Takahashi, M.; Sekine, M.; Baba, S.; Ankai, A.; Kosugi, H.; Hosoyama, A.; Fukui, S.; Nagai, Y.; Nishijima, K.; Nakazawa, H.; Takamiya, M.; Masuda, S.; Funahashi, T.; Tanaka, T.; Kudoh, Y.; Yamazaki, J.; Kushida, N.; Oguchi, A.; Kikuchi, H.; et al., (1999) Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1. DNA Res. 6(2): 83-101, 145-52.. PubMed ID: 10382966
576.  (INT) Kawarabayasi, Y. (2001) Genome of Pyrococcus horikoshii OT3. Methods Enzymol. 330: 124-34.. PubMed ID: 11210494
577.  (INT) Kawasaki, M.; Makino, S.; Matsuzawa, H.; Satow, Y.; Ohya, Y.; Anraku, Y. (1996) Folding-dependent in vitro protein splicing of the Saccharomyces cerevisiae VMA1 protozyme. Biochem Biophys Res Commun 222(3): 827-32. PubMed ID: 8651930
578.  (EN) (INT) Kawasaki, M.; Nogami, S.; Satow, Y.; Ohya, Y.; Anraku, Y. (1997) Identification of three core regions essential for protein splicing of the yeast Vma1 protozyme. A random mutagenesis study of the entire Vma1-derived endonuclease sequence. J Biol Chem 272(25): 15668-74. PubMed ID: 9188457
579.  (INT) Kawasaki, M.; Satow, Y.; Ohya, Y.; Anraku, Y. (1997B) Protein splicing in the yeast Vma1 protozyme: evidence for an intramolecular reaction. FEBS Lett 412(3): 518-20. PubMed ID: 9276458
580.  (INT) Kawashima, T.; Yamamoto, Y.; Aramaki, H.; Nunoshiba, T.; Kawamoto, T.; Watanabe, K.,; Yamazaki, M.; Kanehori, K.; Amano, N.; Ohya, Y.; Makino, K.; Suzuki, M. (1999) Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1. Proc. Jpn. Acad 75: 213-218.
581.  (EN) (REV) Keeling, P. J.; Roger, A. J. (1995) The selfish pursuit of sex. Nature. 375(6529): 283.. PubMed ID: 7753189
582.  (APP) (PUR) (TS) Kempe, K.; Rubtsova, M.; Gils, M. (2009) Intein-mediated protein assembly in transgenic wheat: production of active barnase and acetolactate synthase from split genes. Plant Biotechnol J 7(3): 283-97. PubMed ID: 19222807
583.  (EPL) (REL) (REV) Kent, S. B. (2009) Total chemical synthesis of proteins. Chem Soc Rev 38(2): 338-51. PubMed ID: 19169452
584.  (INT) Kerrigan, A. M.; Powers, T. L.; Dorval, D. M.; Reitter, J. N.; Mills, K. V. (2009) Protein splicing of the three Pyrococcus abyssi ribonucleotide reductase inteins. Biochem Biophys Res Commun 387(1): 153-7. PubMed ID: 19577540
585.  (INT) Kerrigan, Adam M.; Powers, Taryn L.; Dorval, Deirdre M.; Reitter, Julie N.; Mills, Kenneth V. (2009B) The dependence of three P. abyssi inteins on extein sequence for efficient protein splicing. FASEB J._MeetingAbstracts 22(1_MeetingAbstracts): 611.3.
586.  (INT) Kerrigan, Adam M.; Powers, Taryn L.; Dorval, Deirdre M.; Reitter, Julie N.; Mills, Kenneth V. (2009C) The influence of extein residues on the protein splicing of three Pyrococcus abyssi inteins. FASEB J._MeetingAbstracts 23(1_MeetingAbstracts): 502.3.
587.  (APP) (REV) (TS) Khan, M. S.; Khalid, A. M.; Malik, K. A. (2005) Intein-mediated protein trans-splicing and transgene containment in plastids. Trends Biotechnol 23(5): 217-20. PubMed ID: 15865996
588.  (APP) (EPL) (REV) Khmelnitsky, Yuri L. (2004) Current strategies for in vitro protein glycosylation. Journal of Molecular Catalysis B: Enzymatic 31(4-6): 73-81.
589.  (APP) (PUR) Khodak, Y. A.; Koroleva, O. N.; Drutsa, V. L. (2007) A System for Heterologous Expression and Isolation of Escherichia coli RNA Polymerase and Its Components. Biochemistry (Mosc) 72(2): 178-87. PubMed ID: 17367295
590.  (APP) (PUR) (Structure) Kim, J. J.; Rho, S. H.; Im, Y. J.; Kim, E. J.; Eom, S. H. (2001) Crystallization and preliminary X-ray diffraction studies of the guanylate kinase-like domain of PSD-95 protein from rat. Acta Crystallogr D Biol Crystallogr 57(Pt 4): 616-7. PubMed ID: 11264600
591.  (APP) (TAG) (TS) Kim, S. B.; Ozawa, T.; Watanabe, S.; Umezawa, Y. (2004) High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase. Proc Natl Acad Sci U S A 101(32): 11542-7. PubMed ID: 15289615
592.  (APP) (TAG) (TS) Kim, S. B.; Ozawa, T.; Umezawa, Y. (2005) Genetically encoded stress indicator for noninvasively imaging endogenous corticosterone in living mice. Anal Chem 77(20): 6588-93. PubMed ID: 16223244
593.  (APP) (TAG) (TS) Kim, S. B.; Takao, R.; Ozawa, T.; Umezawa, Y. (2005B) Quantitative determination of protein nuclear transport induced by phosphorylation or by proteolysis. Anal Chem 77(21): 6928-34. PubMed ID: 16255591
594.  (APP) (TAG) (TS) Kim, S. B.; Ozawa, T.; Umezawa, Y. (2005C) A genetically encoded indicator for assaying bioactive chemicals that induce nuclear transport of glucocorticoid receptor. Anal Biochem 347(2): 213-20. PubMed ID: 16269125
595.  (INT) Kim, Y. J.; Lee, H. S.; Bae, S. S.; Jeon, J. H.; Lim, J. K.; Cho, Y.; Nam, K. H.; Kang, S. G.; Kim, S. J.; Kwon, S. T.; Lee, J. H. (2007) Cloning, purification, and characterization of a new DNA polymerase from a hyperthermophilic archaeon, Thermococcus sp. NA1. J Microbiol Biotechnol 17(7): 1090-7. PubMed ID: 18051318
596.  (APP) (PUR) Kim, G. A.; Lee, M. S.; Sun, Y.; Lee, B. D.; Lee, J. I.; Lee, J. H.; Kwon, S. T. (2008) Characterization of cold-active uracil-DNA glycosylase from Bacillus sp. HJ171 and its use for contamination control in PCR. Appl Microbiol Biotechnol 80(5): 785-94. PubMed ID: 18626641
597.  (APP) (EPL) (TAG) Kim, J.; Guermah, M.; McGinty, R. K.; Lee, J. S.; Tang, Z.; Milne, T. A.; Shilatifard, A.; Muir, T. W.; Roeder, R. G. (2009) RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells. Cell 137(3): 459-71. PubMed ID: 19410543
598.  (APP) (EPL) (TAG) Kim, S.; Sun, H.; Ball, H. L.; Wassmann, K.; Luo, X.; Yu, H. (2010) Phosphorylation of the spindle checkpoint protein Mad2 regulates its conformational transition. Proc Natl Acad Sci U S A (IN PRESS). PubMed ID: 21041666
599.  (APP) (EPL) (REV) Kimmerlin, T.; Seebach, D. (2005) '100 years of peptide synthesis': ligation methods for peptide and protein synthesis with applications to beta-peptide assemblies. J Pept Res 65(2): 229-60. PubMed ID: 15705167
600.  (APP) (CYCL) (EPL) (REV) Kimura, R.; Camarero, J. A. (2005) Expressed protein ligation: a new tool for the biosynthesis of cyclic polypeptides. Protein Pept Lett 12(8): 789-94. PubMed ID: 16305550
601.  (APP) (CYCL) Kimura, R. H.; Tran, A. T.; Camarero, J. A. (2006) Biosynthesis of the cyclotide kalata B1 by using protein splicing. Angew Chem Int Ed Engl 45(6): 973-6. PubMed ID: 16381044
602.  (APP) (CYCL) (PUR) Kinsella, T. M.; Ohashi, C. T.; Harder, A. G.; Yam, G. C.; Li, W.; Peelle, B.; Pali, E. S.; Bennett, M. K.; Molineaux, S. M.; Anderson, D. A.; Masuda, E. S.; Payan, D. G. (2002) Retrovirally delivered random cyclic Peptide libraries yield inhibitors of interleukin-4 signaling in human B cells. J Biol Chem 277(40): 37512-8. PubMed ID: 12167667
603.  (APP) Kinsland, C.; Taylor, S. V.; Kelleher, N. L.; McLafferty, F. W.; Begley, T. P. (1998) Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis. Protein Sci 7(8): 1839-42. PubMed ID: 10082383
604.  (APP) (PUR) Kiraly, O.; Guan, L.; Szepessy, E.; Toth, M.; Kukor, Z.; Sahin-Toth, M. (2006) Expression of human cationic trypsinogen with an authentic N terminus using intein-mediated splicing in aminopeptidase P deficient Escherichia coli. Protein Expr Purif 48(1): 104-11. PubMed ID: 16542853
605.  (APP) (PUR) Kirkland, B. H.; Keyhani, N. O. (2010) Expression and purification of a functionally active class I fungal hydrophobin from the entomopathogenic fungus Beauveria bassiana in E. coli. J Ind Microbiol Biotechnol (IN PRESS). PubMed ID: 20640587
606.  (APP) (PUR) Kiss, R. S.; Weers, P. M.; Narayanaswami, V.; Cohen, J.; Kay, C. M.; Ryan, R. O. (2003) Structure-guided protein engineering modulates helix bundle exchangeable apolipoprotein properties. J Biol Chem 278(24): 21952-9. PubMed ID: 12684504
607.  (INT) (Structure) Klabunde, T.; Sharma, S.; Telenti, A.; Jacobs, W. R., Jr.; Sacchettini, J. C. (1998) Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing. Nat Struct Biol 5(1): 31-6. PubMed ID: 9437427
608.  (INT) Klenk, H. P.; Clayton, R. A.; Tomb, J. F.; White, O.; Nelson, K. E.; Ketchum, K. A.; Dodson, R. J.; Gwinn, M.; Hickey, E. K.; Peterson, J. D.; Richardson, D. L.; Kerlavage, A. R.; Graham, D. E.; Kyrpides, N. C.; Fleischmann, R. D.; Quackenbush, J.; Lee, N. H.; Sutton, G. G.; Gill, S.; Kirkness, E. F.; Dougherty, B. A.; McKenney, K.; Adams, M. D.; Loftus, B.; Peterson, S.; Reich, C. I.; McNeil, L. K.; Badger, J. H.; Glodek, A.; Zhou, L.; Overbeek, R.; Gocayne, J. D.; Weidman, J. F.; McDonald, L.; Utterback, T.; Cotton, M. D.; Spriggs, T.; Artiach, P.; Kaine, B. P.; Sykes, S. M.; Sadow, P. W.; D'Andrea, K. P.; Bowman, C.; Fujii, C.; Garland, S. A.; Mason, T. M.; Olsen, G. J.; Fraser, C. M.; Smith, H. O.; Woese, C. R.; Venter, J. C. (1997) The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus. Nature 390(6658): 364-70. PubMed ID: 9389475
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610.  (PUR) Kochanowski, R.; Kotlowski, R.; Szweda, P. (2007) Expression and intein-mediated purification of novel staphylokinase SakSTAR with reduced immunogenicity and antiplatelet and antithrombin activation. Appl Biochem Biotechnol 141(2-3): 321-33. PubMed ID: 18025559
611.  (APP) (REV) Kochendoerfer, G. G.; Kent, S. B. (1999) Chemical protein synthesis. Curr Opin Chem Biol 3(6): 665-71. PubMed ID: 10600727
612.  (APP) (EPL) (REL) (REV) Kochendoerfer, G. G. (2001) Chemical protein synthesis methods in drug discovery. Curr Opin Drug Discov Devel. 4(2): 205-14.. PubMed ID: 11378960
613.  (APP) (EPL) (REL) (REV) Kochendoerfer, G. (2003) Chemical and biological properties of polymer-modified proteins. Expert Opin Biol Ther. 3(8): 1253-61.. PubMed ID: 14640951
614.  (APP) (NMR) (REL) (Structure) Kochendoerfer, G. G.; Jones, D. H.; Lee, S.; Oblatt-Montal, M.; Opella, S. J.; Montal, M. (2004) Functional characterization and NMR spectroscopy on full-length Vpu from HIV-1 prepared by total chemical synthesis. J Am Chem Soc. 126(8): 2439-46.. PubMed ID: 14982452
615.  (APP) (EPL) (REL) (REV) Kochendoerfer, G. G. (2005) Site-specific polymer modification of therapeutic proteins. Curr Opin Chem Biol. 9(6): 555-60. Epub 2005 Oct 17.. PubMed ID: 16233987
616.  (APP) (EPL) (NEB) Kochinyan, S.; Sun, L.; Ghosh, I.; Barshevsky, T.; Xu, J.; Xu, M. Q. (2007) Use of intein-mediated phosphoprotein arrays to study substrate specificity of protein phosphatases. Biotechniques 42(1): 63-9. PubMed ID: 17269486
617.  (APP) (EPL) (REV) Koeller, K. M.; Wong, C. H. (2000) Complex carbohydrate synthesis tools for glycobiologists: enzyme-based approach and programmable one-pot strategies. Glycobiology. 10(11): 1157-69.. PubMed ID: 11087708
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619.  (APP) (EPL) (REV) (TAG) Komarov, A. G.; Linn, K. M.; Devereaux, J. J.; Valiyaveetil, F. I. (2009) Semisynthesis of K+ channels. Methods Enzymol 462: 135-50. PubMed ID: 19632473
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624.  (APP) (EPL) (TAG) Korona, Dagmara; Korona, Boguslawa; Wojcik, Edward (2009) Intein-mediated protein splicing of human Eg5 kinesin. FASEB J._MeetingAbstracts 23(1_MeetingAbstracts): 502.19.
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630.  (APP) (EPL) (PUR) Kumar Bhat, R.; Berger, S. (2007) New and easy strategy for cloning, expression, purification, and characterization of the 5S subunit of transcarboxylase from Propionibacterium f. shermanii. Prep Biochem Biotechnol 37(1): 13-26. PubMed ID: 17134979
631.  (APP) (PUR) Kunanopparat, A.; Chaivisuthangkura, P.; Senapin, S.; Longyant, S.; Rukpratanporn, S.; Flegel, T. W.; Sithigorngul, P. (2010) Detection of infectious myonecrosis virus using monoclonal antibody specific to N and C fragments of the capsid protein expressed heterologously. J Virol Methods (IN PRESS). PubMed ID: 21029750
632.  (APP) (PUR) Kunes, Y. Z.; Gion, W. R.; Fung, E.; Salfeld, J. G.; Zhu, R. R.; Sakorafas, P.; Carson, G. R. (2009) Expression of antibodies using single-open reading frame vector design and polyprotein processing from mammalian cells. Biotechnol Prog 25(3): 735-44. PubMed ID: 19363814
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634.  (APP) (TAG) (TS) Kurpiers, T.; Mootz, H. D. (2007) Regioselective Cysteine Bioconjugation by Appending a Labeled Cystein Tag to a Protein by Using Protein Splicing in trans. Angew Chem Int Ed Engl 46(27): 5234-5237. PubMed ID: 17538921
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636.  (APP) (PUR) (REV) (TAG) Kusnezow, W.; Hoheisel, J. D. (2003) Solid supports for microarray immunoassays. J Mol Recognit 16(4): 165-76. PubMed ID: 12898667
637.  (APP) (PUR) Kutchma, A. J.; Hoang, T. T.; Schweizer, H. P. (1999) Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (FabD). J Bacteriol 181(17): 5498-504. PubMed ID: 10464226
638.  (INT) Kwan, T.; Liu, J.; DuBow, M.; Gros, P.; Pelletier, J. (2005) The complete genomes and proteomes of 27 Staphylococcus aureus bacteriophages. Proc Natl Acad Sci U S A. 102(14): 5174-9. Epub 2005 Mar 23.. PubMed ID: 15788529
639.  (APP) (TAG) (TS) Kwon, Y.; Coleman, M. A.; Camarero, J. A. (2006) Selective Immobilization of Proteins onto Solid Supports through Split-Intein-Mediated Protein Trans-Splicing. Angew Chem Int Ed Engl 45(11): 1726-1729. PubMed ID: 16470557
640.  (APP) (CYCL) (TS) Kwon, J. S.; Bal, J.; Hwang, H. M.; Kim, J. Y. (2008) A circularly permuted beta-lactamase as a novel reporter for evaluation of protein cyclization efficiency. J Microbiol 46(4): 456-61. PubMed ID: 18758738
641.  (APP) (PUR) Lacey, B. M.; Hondal, R. J. (2006) Characterization of mitochondrial thioredoxin reductase from C. elegans. Biochem Biophys Res Commun 346(3): 629-36. PubMed ID: 16780799
642.  (APP) (PUR) (TAG) Laitem, C.; Choul-Li, S.; Baillat, D.; Begue, A.; Aumercier, M. (2008) Efficient system for biotinylated recombinant Ets-1 production in Escherichiacoli: a useful tool for studying interactions between Ets-1 and its partners. Protein Expr Purif 62(1): 53-63. PubMed ID: 18639639
643.  (REL) (REV) Lambowitz, A. M.; Belfort, M. (1993) Introns as mobile genetic elements. Annu Rev Biochem 62: 587-622. PubMed ID: 8352597
644.  (APP) (REV) (TAG) Lay, P. T.; Yao, S. Q. (2005) Intein-mediated, in vitro and in vivo protein modifications with small molecules. Protein and Peptide Letters 12(8): 769-775.
645.  (INT) Lazarevic, V.; Soldo, B.; Dusterhoft, A.; Hilbert, H.; Mauel, C.; Karamata, D. (1998) Introns and intein coding sequence in the ribonucleotide reductase genes of Bacillus subtilis temperate bacteriophage SPbeta. Proc Natl Acad Sci U S A 95(4): 1692-7. PubMed ID: 9465078
646.  (INT) Lazarevic, V. (2001) Ribonucleotide reductase genes of Bacillus prophages: a refuge to introns and intein coding sequences. Nucleic Acids Res 29(15): 3212-8. PubMed ID: 11470879
647.  (INT) Lecompte, O.; Ripp, R.; Puzos-Barbe, V.; Duprat, S.; Heilig, R.; Dietrich, J.; Thierry, J. C.; Poch, O. (2001) Genome evolution at the genus level: comparison of three complete genomes of hyperthermophilic archaea. Genome Res. 11(6): 981-93.. PubMed ID: 11381026
648.  (REL) Lee, J. J.; Ekker, S. C.; von Kessler, D. P.; Porter, J. A.; Sun, B. I.; Beachy, P. A. (1994) Autoproteolysis in hedgehog protein biogenesis. Science. 266(5190): 1528-37.. PubMed ID: 7985023
649.  (APP) (PUR) (Structure) (TAG) Lee, N. Y.; Hazlett, T. L.; Koland, J. G. (2006) Structure and dynamics of the epidermal growth factor receptor C-terminal phosphorylation domain. Protein Sci 15(5): 1142-52. PubMed ID: 16597832
650.  (APP) (PUR) Lee, M. S.; Kim, G. A.; Seo, M. S.; Lee, J. H.; Kwon, S. T. (2009) Characterization of heat-labile uracil-DNA glycosylase from Psychrobacter sp. HJ147 and its application to the polymerase chain reaction. Biotechnol Appl Biochem 52(Pt 2): 167-75. PubMed ID: 18412541
651.  (APP) (EPL) (TAG) Lesaicherre, M. L.; Lue, R. Y.; Chen, G. Y.; Zhu, Q.; Yao, S. Q. (2002) Intein-mediated biotinylation of proteins and its application in a protein microarray. J Am Chem Soc 124(30): 8768-9. PubMed ID: 12137509
652.  (APP) (REV) Lesley, S. A. (2001) High-throughput proteomics: protein expression and purification in the postgenomic world. Protein Expr Purif 22(2): 159-64. PubMed ID: 11437590
653.  (APP) (EPL) Levengood, M. R.; Kerwood, C. C.; Chatterjee, C.; van der Donk, W. A. (2009) Investigation of the substrate specificity of lacticin 481 synthetase by using nonproteinogenic amino acids. Chembiochem 10(5): 911-9. PubMed ID: 19222036
654.  (APP) (CPS) (TS) Lew, B. M.; Mills, K. V.; Paulus, H. (1998) Protein splicing in vitro with a semisynthetic two-component minimal intein. J Biol Chem 273(26): 15887-90. PubMed ID: 9632632
655.  (APP) (CPS) (INT) (TS) Lew, B. M.; Mills, K. V.; Paulus, H. (1999) Characteristics of protein splicing in trans mediated by a semisynthetic split intein. Biopolymers 51(5): 355-62. PubMed ID: 10685046
656.  (APP) (CPS) Lew, B. M.; Paulus, H. (2002) An in vivo screening system against protein splicing useful for the isolation of non-splicing mutants or inhibitors of the RecA intein of Mycobacterium tuberculosis. Gene 282(1-2): 169-77. PubMed ID: 11814689
657.  (REL) Li, Y.; Zhao, Y.; Hatfield, S.; Wan, R.; Zhu, Q.; Li, X.; McMills, M.; Ma, Y.; Li, J.; Brown, K. L.; He, C.; Liu, F.; Chen, X. (2000) Dipeptide seryl-histidine and related oligopeptides cleave DNA, protein, and a carboxyl ester. Bioorg Med Chem 8(12): 2675-80. PubMed ID: 11131157
658.  (APP) (PUR) Li, H. H.; Thomas, M. J.; Pan, W.; Alexander, E.; Samuel, M.; Sorci-Thomas, M. G. (2001) Preparation and incorporation of probe-labeled apoA-I for fluorescence resonance energy transfer studies of rHDL. J Lipid Res 42(12): 2084-91. PubMed ID: 11734582
659.  (APP) (PUR) Li, Z. Y.; Fan, J. H.; Yuan, J. M. (2002) Single-column purification of recombinant human neurotrophin-3 (hNT3) by using the intein mediated self-cleaving system. Biotechnology Letters 24(20): 1723-1727.
660.  (APP) (CPS) (TS) Li, J.; Sun, W.; Wang, B.; Xiao, X.; Liu, X. Q. (2008) Protein trans-splicing as a means for viral vector-mediated in vivo gene therapy. Hum Gene Ther 19(9): 958-64. PubMed ID: 18788906
661.  (APP) (EPL) (TAG) Li, J.; Taylor, I. A.; Lloyd, J.; Clapperton, J. A.; Howell, S.; MacMillan, D.; Smerdon, S. J. (2008B) Chk2 oligomerization studied by phosphopeptide ligation: implications for regulation and phosphodependent interactions. J Biol Chem 283(51): 36019-30. PubMed ID: 18948271
662.  (EN) Li, H.; Pellenz, S.; Ulge, U.; Stoddard, B. L.; Monnat, R. J., Jr. (2009) Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins. Nucleic Acids Res 37(5): 1650-62. PubMed ID: 19153140
663.  (APP) (EPL) (REL) (TAG) Li, X.; Fekner, T.; Ottesen, J. J.; Chan, M. K. (2009) A pyrrolysine analogue for site-specific protein ubiquitination. Angew Chem Int Ed Engl 48(48): 9184-7. PubMed ID: 19882608
664.  (APP) (CPS) (PUR) Liang, R.; Liu, X.; Liu, J.; Ren, Q.; Liang, P.; Lin, Z.; Xie, X. (2007) A T7-expression system under temperature control could create temperature-sensitive phenotype of target gene in Escherichia coli. J Microbiol Methods 68(3): 497-506. PubMed ID: 17169451
665.  (APP) (PUR) (REV) Lichty, J. J.; Malecki, J. L.; Agnew, H. D.; Michelson-Horowitz, D. J.; Tan, S. (2005) Comparison of affinity tags for protein purification. Protein Expr Purif. 41(1): 98-105.. PubMed ID: 15802226
666.  (APP) (EPL) (REV) Lin, Michael Z.; Wang, Lei (2008) Selective Labeling of Proteins with Chemical Probes in Living Cells. Physiology 23(3): 131-141.
667.  (APP) (CYCL) (PUR) (TS) Lin, Z.; Wang, S.; Yang, X.; Yang, K. (2008) [Construction and evaluation of DnaB split intein high expression vector and a six amino acids cyclic peptide library]. Sheng Wu Gong Cheng Xue Bao 24(11): 1924-30. PubMed ID: 19256340
668.  (REL) (REV) Little, J. W. (1993) LexA cleavage and other self-processing reactions. J Bacteriol. 175(16): 4943-50.. PubMed ID: 8349538
669.  (INT) Liu, X. Q.; Hu, Z. (1997) Identification and characterization of a cyanobacterial DnaX intein. FEBS Lett 408(3): 311-4. PubMed ID: 9188783
670.  (EN) (INT) Liu, X. Q.; Hu, Z. (1997B) A DnaB intein in Rhodothermus marinus: indication of recent intein homing across remotely related organisms. Proc Natl Acad Sci U S A 94(15): 7851-6. PubMed ID: 9223276
671.  (INT) (REV) Liu, X. Q. (2000) Protein-splicing intein: Genetic mobility, origin, and evolution. Annu Rev Genet 34: 61-76. PubMed ID: 11092822
672.  (APP) (PUR) Liu, C.; Shim, J. H.; Benkovic, S. J. (2000B) The Unexpected Catalytic Properties of a Heterodimer of GAR Transformylase. Bioorg Chem 28(6): 316-323.
673.  (INT) (TS) Liu, X. Q.; Yang, J. (2003) Split dnaE genes encoding multiple novel inteins in Trichodesmium erythraeum. J Biol Chem 278(29): 26315-8. PubMed ID: 12766150
674.  (APP) (CYCL) (PUR) Liu, C. W.; Corboy, M. J.; DeMartino, G. N.; Thomas, P. J. (2003) Endoproteolytic activity of the proteasome. Science 299(5605): 408-11. PubMed ID: 12481023
675.  (INT) Liu, X. Q.; Yang, J.; Meng, Q. (2003B) Four inteins and three group II introns encoded in a bacterial ribonucleotide reductase gene. J Biol Chem 278(47): 46826-31. PubMed ID: 12975359
676.  (EN) (INT) Liu, X. Q.; Yang, J. (2004) Prp8 intein in fungal pathogens: target for potential antifungal drugs. FEBS Lett 572(1-3): 46-50. PubMed ID: 15304322
677.  (INT) Liu, X. Q.; Yang, J. (2004B) Bacterial thymidylate synthase with intein, group II Intron, and distinctive ThyX motifs. J Bacteriol 186(18): 6316-9. PubMed ID: 15342603
678.  (APP) (EPL) Liu, D.; Xu, R.; Dutta, K.; Cowburn, D. (2008) N-terminal cysteinyl proteins can be prepared using thrombin cleavage. FEBS Lett 582(7): 1163-7. PubMed ID: 18331839
679.  (APP) (PUR) Liu, J. R.; Duan, C. H.; Zhao, X.; Tzen, J. T.; Cheng, K. J.; Pai, C. K. (2008B) Cloning of a rumen fungal xylanase gene and purification of the recombinant enzyme via artificial oil bodies. Appl Microbiol Biotechnol 79(2): 225-33. PubMed ID: 18415096
680.  (APP) (NMR) (REV) Liu, D.; Xu, R.; Cowburn, D. (2009) Segmental isotopic labeling of proteins for nuclear magnetic resonance. Methods Enzymol 462: 151-75. PubMed ID: 19632474
681.  (APP) (INT) (TS) Lockless, S. W.; Muir, T. W. (2009) Traceless protein splicing utilizing evolved split inteins. Proc Natl Acad Sci U S A 106(27): 10999-1004. PubMed ID: 19541616
682.  (INT) Loftus, B. J.; Fung, E.; Roncaglia, P.; Rowley, D.; Amedeo, P.; Bruno, D.; Vamathevan, J.; Miranda, M.; Anderson, I. J.; Fraser, J. A.; Allen, J. E.; Bosdet, I. E.; Brent, M. R.; Chiu, R.; Doering, T. L.; Donlin, M. J.; D'Souza, C. A.; Fox, D. S.; Grinberg, V.; Fu, J.; Fukushima, M.; Haas, B. J.; Huang, J. C.; Janbon, G.; Jones, S. J.; Koo, H. L.; Krzywinski, M. I.; Kwon-Chung, J. K.; Lengeler, K. B.; Maiti, R.; Marra, M. A.; Marra, R. E.; Mathewson, C. A.; Mitchell, T. G.; Pertea, M.; Riggs, F. R.; Salzberg, S. L.; Schein, J. E.; Shvartsbeyn, A.; Shin, H.; Shumway, M.; Specht, C. A.; Suh, B. B.; Tenney, A.; Utterback, T. R.; Wickes, B. L.; Wortman, J. R.; Wye, N. H.; Kronstad, J. W.; Lodge, J. K.; Heitman, J.; Davis, R. W.; Fraser, C. M.; Hyman, R. W. (2005) The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans. Science. 307(5713): 1321-4. Epub 2005 Jan 13.. PubMed ID: 15653466
683.  (APP) (EPL) (REV) (TAG) Love, K. R.; Catic, A.; Schlieker, C.; Ploegh, H. L. (2007) Mechanisms, biology and inhibitors of deubiquitinating enzymes. Nat Chem Biol 3(11): 697-705. PubMed ID: 17948018
684.  (APP) (EPL) (TAG) Lovrinovic, M.; Seidel, R.; Wacker, R.; Schroeder, H.; Seitz, O.; Engelhard, M.; Goody, R. S.; Niemeyer, C. M. (2003) Synthesis of protein-nucleic acid conjugates by expressed protein ligation. Chem Commun (Camb) (7): 822-3. PubMed ID: 12739629
685.  (APP) (EPL) (TAG) Lovrinovic, M.; Niemeyer, C. M. (2005) Rapid synthesis of DNA-cysteine conjugates for expressed protein ligation. Biochem Biophys Res Commun 335(3): 943-8. PubMed ID: 16102730
686.  (APP) (EPL) (TAG) Lovrinovic, M.; Spengler, M.; Deutsch, C.; Niemeyer, C. M. (2005B) Synthesis of covalent DNA-protein conjugates by expressed protein ligation. Mol Biosyst 1(1): 64-9. PubMed ID: 16880965
687.  (APP) (EPL) (PUR) (TAG) Lovrinovic, M.; Niemeyer, C. M. (2007) Microtiter Plate-Based Screening for the Optimization of DNA-Protein Conjugate Synthesis by Means of Expressed Protein Ligation. Chembiochem 8(1): 61-67. PubMed ID: 17121402
688.  (APP) (EPL) (TAG) Lovrinovic, M.; Fruk, L.; Schroder, H.; Niemeyer, C. M. (2007B) Site-specific labeling of DNA-protein conjugates by means of expressed protein ligation. Chem Commun (Camb) 4: 353-355. PubMed ID: 17220968
689.  (APP) (EPL) Lu, W.; Gong, D.; Bar-Sagi, D.; Cole, P. A. (2001) Site-specific incorporation of a phosphotyrosine mimetic reveals a role for tyrosine phosphorylation of SHP-2 in cell signaling. Mol Cell 8(4): 759-69. PubMed ID: 11684012
690.  (APP) (EPL) Lu, W.; Shen, K.; Cole, P. A. (2003) Chemical dissection of the effects of tyrosine phosphorylation of SHP-2. Biochemistry 42(18): 5461-8. PubMed ID: 12731888
691.  (APP) (REL) Lu, M.; Min, T.; Eliezer, D.; Wu, H. (2006) Native chemical ligation in covalent caspase inhibition by p35. Chem Biol 13(2): 117-22. PubMed ID: 16492559
692.  (APP) (EPL) Lu, X.; Olsen, S. K.; Capili, A. D.; Cisar, J. S.; Lima, C. D.; Tan, D. S. (2010) Designed semisynthetic protein inhibitors of ub/ubl e1 activating enzymes. J Am Chem Soc 132(6): 1748-9. PubMed ID: 20099854
693.  (APP) (PUR) Lu, Shuo; Zgurskaya, Helen I (2010) Reconstitution of macrolide pump MacAB-TolC into lipid nanodiscs. FASEB J. 24(1_MeetingAbstracts): 699.3-.
694.  (APP) (PUR) Lu, M.; Ngo, W.; Mei, Y.; Munshi, V.; Burlein, C.; Loughran, M. H.; Williams, P. D.; Hazuda, D. J.; Miller, M. D.; Grobler, J. A.; Diamond, T. L.; Lai, M. T. (2010B) Purification of untagged HIV-1 reverse transcriptase by affinity chromatography. Protein Expr Purif 71(2): 231-239. PubMed ID: 20060474
695.  (EN) Lucas, P.; Otis, C.; Mercier, J. P.; Turmel, M.; Lemieux, C. (2001) Rapid evolution of the DNA-binding site in LAGLIDADG homing endonucleases. Nucleic Acids Res. 29(4): 960-9.. PubMed ID: 11160929
696.  (APP) (PUR) (TS) Ludwig, C.; Pfeiff, M.; Linne, U.; Mootz, H. D. (2006) Ligation of a synthetic peptide to the N terminus of a recombinant protein using semisynthetic protein trans-splicing. Angew Chem Int Ed Engl 45(31): 5218-21. PubMed ID: 16823791
697.  (CPS) (INT) (TS) Ludwig, C.; Schwarzer, D.; Mootz, H. D. (2008) Interaction studies and alanine scanning analysis of a semi-synthetic split intein reveal thiazoline ring formation from an intermediate of the protein splicing reaction. J Biol Chem 283(37): 25264-72. PubMed ID: 18625708
698.  (APP) (TS) Ludwig, C.; Schwarzer, D.; Zettler, J.; Garbe, D.; Janning, P.; Czeslik, C.; Mootz, H. D. (2009) Semisynthesis of proteins using split inteins. Methods Enzymol 462: 77-96. PubMed ID: 19632470
699.  (APP) (EPL) (TAG) Lue, R. Y.; Chen, G. Y.; Hu, Y.; Zhu, Q.; Yao, S. Q. (2004) Versatile protein biotinylation strategies for potential high-throughput proteomics. J Am Chem Soc 126(4): 1055-62. PubMed ID: 14746473
700.  (APP) (EPL) (TAG) Lue, R. Y.; Chen, G. Y.; Zhu, Q.; Lesaicherre, M. L.; Yao, S. Q. (2004B) Site-specific immobilization of biotinylated proteins for protein microarray analysis. Methods Mol Biol 264: 85-100. PubMed ID: 15020782
701.  (APP) (PUR) Luka, Z.; Wagner, C. (2003) Expression and purification of glycine N-methyltransferases in Escherichia coli. Protein Expr Purif 28(2): 280-6. PubMed ID: 12699692
702.  (INT) Luo, J.; Hall, B. D. (2007) A multistep process gave rise to RNA polymerase IV of land plants. J Mol Evol 64(1): 101-12. PubMed ID: 17160640
703.  (APP) (PUR) Luttkopf, D.; Muller, U.; Skov, P. S.; Ballmer-Weber, B. K.; Wuthrich, B.; Skamstrup Hansen, K.; Poulsen, L. K.; Kastner, M.; Haustein, D.; Vieths, S. (2002) Comparison of four variants of a major allergen in hazelnut (Corylus avellana) Cor a 1.04 with the major hazel pollen allergen Cor a 1.01. Mol Immunol 38(7): 515-25. PubMed ID: 11750653
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707.  (APP) (PUR) Ma, J.; Cooney, C. L. (2004) Application of vortex flow adsorption technology to intein-mediated recovery of recombinant human alpha1-antitrypsin. Biotechnol Prog 20(1): 269-76. PubMed ID: 14763852
708.  (APP) (PUR) Ma, C.; Gao, M.; Liu, W.; Zhu, J.; Tian, H.; Gao, X.; Yao, W. (2010) Intein-mediated expression and purification of an analog of glucagon-like peptide-1 in Escherichia coli. Protein Pept Lett 17(10): 1245-50. PubMed ID: 20441562
709.  (APP) (EPL) (TAG) Maag, D.; Lorsch, J. R. (2003) Communication between eukaryotic translation initiation factors 1 and 1A on the yeast small ribosomal subunit. J Mol Biol 330(5): 917-24. PubMed ID: 12860115
710.  (APP) (EPL) (TAG) Maag, D.; Fekete, C. A.; Gryczynski, Z.; Lorsch, J. R. (2005) A conformational change in the eukaryotic translation preinitiation complex and release of eIF1 signal recognition of the start codon. Mol Cell 17(2): 265-75. PubMed ID: 15664195
711.  (APP) (PUR) Machova, Z.; Muhle, C.; Krauss, U.; Trehin, R.; Koch, A.; Merkle, H. P.; Beck-Sickinger, A. G. (2002) Cellular internalization of enhanced green fluorescent protein ligated to a human calcitonin-based carrier peptide. Chembiochem 3(7): 672-7. PubMed ID: 12325002
712.  (APP) (EPL) Machova, Z.; Von Eggelkraut-Gottanka, R.; Wehofsky, N.; Bordusa, F.; Beck-Sickinger, A. G. (2003) Expressed Enzymatic Ligation for the Semisynthesis of Chemically Modified Proteins. Angewandte Chemie - International Edition 42(40): 4916-4918.
713.  (APP) (EPL) (REV) Machova, Z.; Beck-Sickinger, A. G. (2005) Expressed protein ligation for protein semisynthesis and engineering. Methods Mol Biol 298: 105-30. PubMed ID: 16044543
714.  (APP) (EPL) (REV) Macmillan, Derek; Bertozzi, Carolyn R. (2000) New Directions in Glycoprotein Engineering. Tetrahedron 56(48): 9515-9525.
715.  (APP) (EPL) Macmillan, D.; Arham, L. (2004) Cyanogen bromide cleavage generates fragments suitable for expressed protein and glycoprotein ligation. J Am Chem Soc 126(31): 9530-1. PubMed ID: 15291543
716.  (APP) (EPL) Macmillan, D.; Bertozzi, C. R. (2004B) Modular assembly of glycoproteins: towards the synthesis of GlyCAM-1 by using expressed protein ligation. Angew Chem Int Ed Engl 43(11): 1355-9. PubMed ID: 15368405
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726.  (APP) (EPL) (TAG) Manohar, M.; Mooney, A. M.; North, J. A.; Nakkula, R. J.; Picking, J. W.; Edon, A.; Fishel, R.; Poirier, M. G.; Ottesen, J. J. (2009) Acetylation of histone H3 at the nucleosome dyad alters DNA-histone binding. J Biol Chem 284(35): 23312-21. PubMed ID: 19520870
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740.  (APP) (EPL) McGinty, R. K.; Kim, J.; Chatterjee, C.; Roeder, R. G.; Muir, T. W. (2008) Chemically ubiquitylated histone H2B stimulates hDot1L-mediated intranucleosomal methylation. Nature 453(7196): 812-6. PubMed ID: 18449190
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750.  (APP) (EPL) Miller, J. S.; Dudkin, V. Y.; Lyon, G. J.; Muir, T. W.; Danishefsky, S. J. (2003) Toward fully synthetic N-linked glycoproteins. Angew Chem Int Ed Engl. 42(4): 431-4.. PubMed ID: 12569509
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754.  (INT) Mills, K. V.; Manning, J. S.; Garcia, A. M.; Wuerdeman, L. A. (2004) Protein splicing of a Pyrococcus abyssi intein with a C-terminal glutamine. J Biol Chem 279(20): 20685-91. PubMed ID: 15024006
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823.  (APP) (PUR) Nentwich, S. S.; Brinkrolf, K.; Gaigalat, L.; Huser, A. T.; Rey, D. A.; Mohrbach, T.; Marin, K.; Puhler, A.; Tauch, A.; Kalinowski, J. (2009) Characterization of the LacI-type transcriptional repressor RbsR controlling ribose transport in Corynebacterium glutamicum ATCC 13032. Microbiology 155(Pt 1): 150-64. PubMed ID: 19118356
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825.  (APP) (REL) Nguyen, U. T.; Cramer, J.; Gomis, J.; Reents, R.; Gutierrez-Rodriguez, M.; Goody, R. S.; Alexandrov, K.; Waldmann, H. (2007) Exploiting the Substrate Tolerance of Farnesyltransferase for Site-Selective Protein Derivatization. Chembiochem 8(4): 408-423. PubMed ID: 17279592
826.  (CPS) (INT) (NEB) (TS) Nichols, N. M.; Benner, J. S.; Martin, D. D.; Evans, T. C., Jr. (2003) Zinc ion effects on individual Ssp DnaE intein splicing steps: regulating pathway progression. Biochemistry 42(18): 5301-11. PubMed ID: 12731871
827.  (INT) (NEB) (TS) Nichols, N. M.; Evans, T. C., Jr. (2004) Mutational analysis of protein splicing, cleavage, and self-association reactions mediated by the naturally split Ssp DnaE intein. Biochemistry 43(31): 10265-76. PubMed ID: 15287754
828.  (INT) Nie, H.; Yang, F.; Zhang, X.; Yang, J.; Chen, L.; Wang, J.; Xiong, Z.; Peng, J.; Sun, L.; Dong, J.; Xue, Y.; Xu, X.; Chen, S.; Yao, Z.; Shen, Y.; Jin, Q. (2006) Complete genome sequence of Shigella flexneri 5b and comparison with Shigella flexneri 2a. BMC Genomics 7: 173. PubMed ID: 16822325
829.  (INT) Niehaus, F.; Frey, B.; Antranikian, G. (1997) Cloning and characterisation of a thermostable alpha-DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. TY. Gene 204(1-2): 153-8. PubMed ID: 9434178
830.  (APP) (PUR) (REV) Nilsson, J.; Stahl, S.; Lundeberg, J.; Uhlen, M.; Nygren, P. A. (1997) Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expr Purif 11(1): 1-16. PubMed ID: 9325133
831.  (APP) (REL) Nilsson, B. L.; Kiessling, L. L.; Raines, R. T. (2000) Staudinger ligation: a peptide from a thioester and azide. Org Lett. 2(13): 1939-41.. PubMed ID: 10891196
832.  (APP) (REL) Nilsson, B. L.; Kiessling, L. L.; Raines, R. T. (2001) High-yielding Staudinger ligation of a phosphinothioester and azide to form a peptide. Org Lett. 3(1): 9-12.. PubMed ID: 11429880
833.  (APP) (EPL) (REL) Nilsson, B. L.; Hondal, R. J.; Soellner, M. B.; Raines, R. T. (2003) Protein assembly by orthogonal chemical ligation methods. J Am Chem Soc. 125(18): 5268-9.. PubMed ID: 12720426
834.  (APP) (EPL) (REL) (REV) Nilsson, B. L.; Soellner, M. B.; Raines, R. T. (2005) Chemical synthesis of proteins. Annu Rev Biophys Biomol Struct. 34: 91-118.. PubMed ID: 15869385
835.  (APP) (CYCL) Nilsson, L. O.; Louassini, M.; Abel-Santos, E. (2005B) Using siclopps for the discovery of novel antimicrobial peptides and their targets. Protein Pept Lett. 12(8): 795-9.. PubMed ID: 16305551
836.  (REL) (REV) Nishino, T.; Ishino, Y. (2001) [Structural motifs in DNA cleaving enzymes]. Tanpakushitsu Kakusan Koso 46(11 Suppl): 1717-25. PubMed ID: 11579571
837.  (EN) (INT) Nishioka, M.; Fujiwara, S.; Takagi, M.; Imanaka, T. (1998) Characterization of two intein homing endonucleases encoded in the DNA polymerase gene of Pyrococcus kodakaraensis strain KOD1. Nucleic Acids Res 26(19): 4409-12. PubMed ID: 9742242
838.  (EN) (Structure) Niu, Y.; Tenney, K.; Li, H.; Gimble, F. S. (2008) Engineering Variants of the I-SceI Homing Endonuclease with Strand-specific and Site-specific DNA-nicking Activity. Journal of Molecular Biology 382(1): 188-202.
839.  (EN) (INT) Noel, A. J.; Wende, W.; Pingoud, A. (2004) DNA recognition by the homing endonuclease PI-SceI involves a divalent metal ion cofactor-induced conformational change. J Biol Chem 279(8): 6794-804. PubMed ID: 14634013
840.  (INT) Nogami, S.; Satow, Y.; Ohya, Y.; Anraku, Y. (1997) Probing novel elements for protein splicing in the yeast Vma1 protozyme: a study of replacement mutagenesis and intragenic suppression. Genetics 147(1): 73-85. PubMed ID: 9286669
841.  (EN) (INT) Nogami, S.; Fukuda, T.; Nagai, Y.; Yabe, S.; Sugiura, M.; Mizutani, R.; Satow, Y.; Anraku, Y.; Ohya, Y. (2002) Homing at an extragenic locus mediated by VDE (PI-SceI) in Saccharomyces cerevisiae. Yeast 19(9): 773-82. PubMed ID: 12112231
842.  (APP) (REV) (TAG) Nolan, G. P. (2005) Tadpoles by the tail. Nat Methods 2(1): 11-2. PubMed ID: 15782163
843.  (EN) (REL) Nomura, N.; Nomura, Y.; Sussman, D.; Klein, D.; Stoddard, B. L. (2008) Recognition of a common rDNA target site in archaea and eukarya by analogous LAGLIDADG and His-Cys box homing endonucleases. Nucleic Acids Res 36(22): 6988-98. PubMed ID: 18984620
844.  (INT) Nonaka, H.; Keresztes, G.; Shinoda, Y.; Ikenaga, Y.; Abe, M.; Naito, K.; Inatomi, K.; Furukawa, K.; Inui, M.; Yukawa, H. (2006) Complete genome sequence of the dehalorespiring bacterium Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides ethenogenes 195. J Bacteriol 188(6): 2262-74. PubMed ID: 16513756
845.  (APP) (INT) (REV) Noren, C. J.; Wang, J.; Perler, F. B. (2000) Dissecting the Chemistry of Protein Splicing and Its Applications. Angew Chem Int Ed Engl 39(3): 450-466. PubMed ID: 10671234
846.  (APP) (EPL) Nyanguile, O.; Dancik, C.; Blakemore, J.; Mulgrew, K.; Kaleko, M.; Stevenson, S. C. (2003) Synthesis of adenoviral targeting molecules by intein-mediated protein ligation. Gene Ther 10(16): 1362-9. PubMed ID: 12883533
847.  (APP) (INT) (NMR) (Structure) (TS) Oeemig, J. S.; Aranko, A. S.; Djupsjobacka, J.; Heinamaki, K.; Iwai, H. (2009) Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification. FEBS Lett 583(9): 1451-6. PubMed ID: 19344715
848.  (APP) (REL) Offer, J.; Boddy, C. N.; Dawson, P. E. (2002) Extending synthetic access to proteins with a removable acyl transfer auxiliary. J Am Chem Soc. 124(17): 4642-6.. PubMed ID: 11971712
849.  (REL) Ogata, H.; Audic, S.; Barbe, V.; Artiguenave, F.; Fournier, P. E.; Raoult, D.; Claverie, J. M. (2000) Selfish DNA in protein-coding genes of Rickettsia. Science 290(5490): 347-50. PubMed ID: 11030655
850.  (INT) Ogata, H.; Raoult, D.; Claverie, J. M. (2005) A new example of viral intein in Mimivirus. Virol J 2: 8. PubMed ID: 15707490
851.  (INT) Okada, C.; Maegawa, Y.; Yao, M.; Tanaka, I. (2006) Crystal structure of an RtcB homolog protein (PH1602-extein protein) from Pyrococcus horikoshii reveals a novel fold. Proteins 63(4): 1119-22. PubMed ID: 16485279
852.  (EN) (INT) Okuda, Y.; Sasaki, D.; Nogami, S.; Kaneko, Y.; Ohya, Y.; Anraku, Y. (2003) Occurrence, horizontal transfer and degeneration of VDE intein family in Saccharomycete yeasts. Yeast 20(7): 563-73. PubMed ID: 12734795
853.  (APP) (EPL) (Structure) (TAG) Olsen, S. K.; Capili, A. D.; Lu, X.; Tan, D. S.; Lima, C. D. (2010) Active site remodelling accompanies thioester bond formation in the SUMO E1. Nature 463(7283): 906-12. PubMed ID: 20164921
854.  (INT) Omura, S.; Ikeda, H.; Ishikawa, J.; Hanamoto, A.; Takahashi, C.; Shinose, M.; Takahashi, Y.; Horikawa, H.; Nakazawa, H.; Osonoe, T.; Kikuchi, H.; Shiba, T.; Sakaki, Y.; Hattori, M. (2001) Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites. Proc Natl Acad Sci U S A. 98(21): 12215-20. Epub 2001 Sep 25.. PubMed ID: 11572948
855.  (APP) (PUR) Osicka, R.; Prochazkova, K.; Sulc, M.; Linhartova, I.; Havlicek, V.; Sebo, P. (2004) A novel "clip-and-link" activity of repeat in toxin (RTX) proteins from gram-negative pathogens. Covalent protein cross-linking by an Asp-Lys isopeptide bond upon calcium-dependent processing at an Asp-Pro bond. J Biol Chem 279(24): 24944-56. PubMed ID: 15044436
856.  (REL) Ostermeier, M. (2005) Engineering allosteric protein switches by domain insertion. Protein Eng Des Sel 18(8): 359-64. PubMed ID: 16043448
857.  (APP) (NMR) (Structure) (TS) Otomo, T.; Teruya, K.; Uegaki, K.; Yamazaki, T.; Kyogoku, Y. (1999) Improved segmental isotope labeling of proteins and application to a larger protein. J Biomol NMR 14(2): 105-14. PubMed ID: 10427740
858.  (APP) (NMR) (Structure) (TS) Otomo, T.; Ito, N.; Kyogoku, Y.; Yamazaki, T. (1999B) NMR observation of selected segments in a larger protein: central-segment isotope labeling through intein-mediated ligation. Biochemistry 38(49): 16040-4. PubMed ID: 10587426
859.  (APP) (EPL) Ottesen, J. J.; Huse, M.; Sekedat, M. D.; Muir, T. W. (2004) Semisynthesis of phosphovariants of Smad2 reveals a substrate preference of the activated T beta RI kinase. Biochemistry 43(19): 5698-706. PubMed ID: 15134444
860.  (APP) (EPL) (TAG) Ottesen, J. J.; Bar-Dagan, M.; Giovani, B.; Muir, T. W. (2008) An amalgamation of solid phase peptide synthesis and ribosomal peptide synthesis. Biopolymers 90(3): 406-14. PubMed ID: 17636509
861.  (APP) (EPL) Ovaa, H.; Galardy, P. J.; Ploegh, H. L. (2005) Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: synthesis of active site-directed probes. Methods Enzymol 399: 468-78. PubMed ID: 16338376
862.  (APP) (TAG) (TS) Ozawa, T.; Nogami, S.; Sato, M.; Ohya, Y.; Umezawa, Y. (2000) A fluorescent indicator for detecting protein-protein interactions in vivo based on protein splicing. Anal Chem 72(21): 5151-7. PubMed ID: 11080857
863.  (APP) (TAG) (TS) Ozawa, T.; Kaihara, A.; Sato, M.; Tachihara, K.; Umezawa, Y. (2001) Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing. Anal Chem 73(11): 2516-21. PubMed ID: 11403293
864.  (APP) (REV) (TS) Ozawa, T.; Umezawa, Y. (2001B) Detection of protein-protein interactions in vivo based on protein splicing. Curr Opin Chem Biol 5(5): 578-83. PubMed ID: 11578933
865.  (APP) (TAG) (TS) Ozawa, T.; Takeuchi, T. M.; Kaihara, A.; Sato, M.; Umezawa, Y. (2001C) Protein splicing-based reconstitution of split green fluorescent protein for monitoring protein-protein interactions in bacteria: improved sensitivity and reduced screening time. Anal Chem 73(24): 5866-74. PubMed ID: 11791555
866.  (APP) (PUR) (TAG) (TS) Ozawa, T.; Sako, Y.; Sato, M.; Kitamura, T.; Umezawa, Y. (2003) A genetic approach to identifying mitochondrial proteins. Nat Biotechnol 21(3): 287-93. PubMed ID: 12577068
867.  (APP) (TAG) (TS) Ozawa, T.; Nishitani, K.; Sako, Y.; Umezawa, Y. (2005) A high-throughput screening of genes that encode proteins transported into the endoplasmic reticulum in mammalian cells. Nucleic Acids Res 33(4): e34. PubMed ID: 15731327
868.  (APP) (REV) (TS) Ozawa, T.; Umezawa, Y. (2005B) Inteins for Split-Protein Reconstitutions and Their Applications. In: Homing Endonucleases and Inteins, ed. Belfort, M.; Derbyshire, V.; Stoddard, B.L.; Wood, D.W.. (Springer-Verlag, Heidelberg, Germany) Vol. 16 pages 307-324.
869.  (APP) (REV) (TAG) (TS) Ozawa, T. (2005D) Methods of analysis for protein dynamics in living cells based on protein splicing. Bulletin of the Chemical Society of Japan 78(5): 739-751.
870.  (APP) (REV) (TAG) (TS) Ozawa, T. (2006) Designing split reporter proteins for analytical tools. Anal Chim Acta 556(1): 58-68. PubMed ID: 17723331
871.  (APP) (PUR) (TS) Ozawa, T.; Umezawa, Y. (2007) Identification of proteins targeted into the endoplasmic reticulum by cDNA library screening. Methods Mol Biol 390: 269-80. PubMed ID: 17951694
872.  (APP) (TS) Ozawa, T.; Natori, Y.; Sako, Y.; Kuroiwa, H.; Kuroiwa, T.; Umezawa, Y. (2007) A minimal peptide sequence that targets fluorescent and functional proteins into the mitochondrial intermembrane space. ACS Chem Biol 2(3): 176-86. PubMed ID: 17348629
873.  (APP) (PUR) (TS) Ozawa, T.; Umezawa, Y. (2007B) A genetic method to identify mitochondrial proteins in living Mammalian cells. Methods Mol Biol 390: 119-30. PubMed ID: 17951684
874.  (APP) (TAG) (TS) Ozawa, T. (2009) Protein reconstitution methods for visualizing biomolecular function in living cells. Yakugaku Zasshi 129(3): 289-295.
875.  (APP) (EPL) (REL) Pal, G.; Santamaria, F.; Kossiakoff, A. A.; Lu, W. (2003) The first semi-synthetic serine protease made by native chemical ligation. Protein Expr Purif. 29(2): 185-92.. PubMed ID: 12767808
876.  (APP) (EPL) (REV) Panigrahi, K.; Eggen, M.; Maeng, J. H.; Shen, Q.; Berkowitz, D. B. (2009) The alpha,alpha-difluorinated phosphonate L-pSer-analogue: an accessible chemical tool for studying kinase-dependent signal transduction. Chem Biol 16(9): 928-36. PubMed ID: 19778720
877.  (INT) Papavinasasundaram, K. G.; Colston, M. J.; Davis, E. O. (1998) Construction and complementation of a recA deletion mutant of Mycobacterium smegmatis reveals that the intein in Mycobacterium tuberculosis recA does not affect RecA function. Mol Microbiol 30(3): 525-34. PubMed ID: 9822818
878.  (PUR) Park, C. M.; Kim, J. I.; Yang, S. S.; Kang, J. G.; Kang, J. H.; Shim, J. Y.; Chung, Y. H.; Park, Y. M.; Song, P. S. (2000) A second photochromic bacteriophytochrome from Synechocystis sp. PCC 6803: spectral analysis and down-regulation by light. Biochemistry 39(35): 10840-7. PubMed ID: 10978170
879.  (APP) (PUR) Park, S. J.; Seo, M. D.; Lee, S. K.; Ikeda, M.; Longnecker, R.; Lee, B. J. (2005) Expression and characterization of N-terminal domain of Epstein-Barr virus latent membrane protein 2A in Escherichia coli. Protein Expr Purif 41(1): 9-17. PubMed ID: 15802216
880.  (APP) (CPS) (PUR) (REV) Pastuszka, M. K.; Mackay, J. A. (2010) Biomolecular engineering of intracellular switches in eukaryotes. Journal of Drug Delivery Science and Technology 20(3): 163-169.
881.  (APP) (PUR) Paul, R.; Bosch, F. U.; Schafer, K. P. (2001) Overexpression and purification of Helicobacter pylori flavodoxin and induction of a specific antiserum in rabbits. Protein Expr Purif 22(3): 399-405. PubMed ID: 11483001
882.  (INT) Paul, J. H.; Williamson, S. J.; Long, A.; Authement, R. N.; John, D.; Segall, A. M.; Rohwer, F. L.; Androlewicz, M.; Patterson, S. (2005) Complete genome sequence of phiHSIC, a pseudotemperate marine phage of Listonella pelagia. Appl Environ Microbiol 71(6): 3311-20. PubMed ID: 15933034
883.  (APP) (EPL) (TAG) Paulick, M. G.; Wise, A. R.; Forstner, M. B.; Groves, J. T.; Bertozzi, C. R. (2007) Synthetic analogues of glycosylphosphatidylinositol-anchored proteins and their behavior in supported lipid bilayers. J Am Chem Soc 129(37): 11543-50. PubMed ID: 17715922
884.  (APP) (EPL) (TAG) Paulick, M. G.; Forstner, M. B.; Groves, J. T.; Bertozzi, C. R. (2007) A chemical approach to unraveling the biological function of the glycosylphosphatidylinositol anchor. Proc Natl Acad Sci U S A 104(51): 20332-7. PubMed ID: 18077333
885.  (APP) (CPS) (TAG) (TS) Paulmurugan, R.; Umezawa, Y.; Gambhir, S. S. (2002) Noninvasive imaging of protein-protein interactions in living subjects by using reporter protein complementation and reconstitution strategies. Proc Natl Acad Sci U S A 99(24): 15608-13. PubMed ID: 12438689
886.  (INT) Paulsen, I. T.; Press, C. M.; Ravel, J.; Kobayashi, D. Y.; Myers, G. S.; Mavrodi, D. V.; DeBoy, R. T.; Seshadri, R.; Ren, Q.; Madupu, R.; Dodson, R. J.; Durkin, A. S.; Brinkac, L. M.; Daugherty, S. C.; Sullivan, S. A.; Rosovitz, M. J.; Gwinn, M. L.; Zhou, L.; Schneider, D. J.; Cartinhour, S. W.; Nelson, W. C.; Weidman, J.; Watkins, K.; Tran, K.; Khouri, H.; Pierson, E. A.; Pierson, L. S., 3rd; Thomashow, L. S.; Loper, J. E. (2005) Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5. Nat Biotechnol. 23(7): 873-8. Epub 2005 Jun 26.. PubMed ID: 15980861
887.  (INT) (REV) Paulus, H. (1998) Protein splicing: A novel form of gene expression and paradigm for self-catalyzed protein rearrangements. Pure & Appl. Chem. 70: 1-8.
888.  (INT) (REV) Paulus, H. (1998B) The chemical basis of protein splicing. Chemical Society Reviews 27: 375-386.
889.  (INT) (REV) Paulus, H. (2000) Protein splicing and related forms of protein autoprocessing. Annu Rev Biochem 69: 447-96. PubMed ID: 10966466
890.  (INT) (REV) Paulus, H. (2001) Inteins as enzymes. Bioorg Chem 29(3): 119-29. PubMed ID: 11437387
891.  (APP) (REV) Paulus, H. (2003) Inteins as targets for potential antimycobacterial drugs. Front Biosci 8: s1157-65. PubMed ID: 12957838
892.  (APP) (CPS) Paulus, H. (2007) Protein splicing inhibitors as a new class of antimycobacterial agents. Drugs of the Future 32(11): 973-984.
893.  (INT) Pearl, E. J.; Tyndall, J. D.; Poulter, R. T.; Wilbanks, S. M. (2007) Sequence requirements for splicing by the Cne PRP8 intein. FEBS Lett 581(16): 3000-4. PubMed ID: 17544410
894.  (INT) Pearl, E. J.; Bokor, A. A.; Butler, M. I.; Poulter, R. T.; Wilbanks, S. M. (2007B) Preceding hydrophobic and beta-branched amino acids attenuate splicing by the CnePRP8 intein. Biochim Biophys Acta 1774(8): 995-1001. PubMed ID: 17604706
895.  (APP) (REL) (TAG) Pechlivanis, M.; Kuhlmann, J. (2006) Hydrophobic modifications of Ras proteins by isoprenoid groups and fatty acids--More than just membrane anchoring. Biochim Biophys Acta 1764(12): 1914-31. PubMed ID: 17110180
896.  (INT) Pedulla, M. L.; Ford, M. E.; Houtz, J. M.; Karthikeyan, T.; Wadsworth, C.; Lewis, J. A.; Jacobs-Sera, D.; Falbo, J.; Gross, J.; Pannunzio, N. R.; Brucker, W.; Kumar, V.; Kandasamy, J.; Keenan, L.; Bardarov, S.; Kriakov, J.; Lawrence, J. G.; Jacobs, W. R., Jr.; Hendrix, R. W.; Hatfull, G. F. (2003) Origins of highly mosaic mycobacteriophage genomes. Cell. 113(2): 171-82.. PubMed ID: 12705866
897.  (APP) (REL) (REV) Pelletier, J.; Sidhu, S. (2001) Mapping protein-protein interactions with combinatorial biology methods. Curr Opin Biotechnol. 12(4): 340-7.. PubMed ID: 11551461
898.  (APP) (EPL) (TAG) Pellois, J. P.; Hahn, M. E.; Muir, T. W. (2004) Simultaneous triggering of protein activity and fluorescence. J Am Chem Soc. 126(23): 7170-1.. PubMed ID: 15186142
899.  (APP) Pellois, J. P.; Muir, T. W. (2005) A ligation and photorelease strategy for the temporal and spatial control of protein function in living cells. Angew Chem Int Ed Engl 44(35): 5713-7. PubMed ID: 16059958
900.  (APP) (EPL) (REV) Pellois, J. P.; Muir, T. W. (2006) Semisynthetic proteins in mechanistic studies: using chemistry to go where nature can't. Curr Opin Chem Biol 10(5): 487-91. PubMed ID: 16935551
901.  (APP) (REL) Pentelute, B. L.; Kent, S. B. (2007) Selective desulfurization of cysteine in the presence of Cys(Acm) in polypeptides obtained by native chemical ligation. Org Lett 9(4): 687-90. PubMed ID: 17286375
902.  (EN) (INT) (NEB) Perler, F. B.; Comb, D. G.; Jack, W. E.; Moran, L. S.; Qiang, B.; Kucera, R. B.; Benner, J.; Slatko, B. E.; Nwankwo, D. O.; Hempstead, S. K.; et al., (1992) Intervening sequences in an Archaea DNA polymerase gene. Proc Natl Acad Sci U S A. 89(12): 5577-81.. PubMed ID: 1608969
903.  (INT) (NEB) Perler, F. B.; Davis, E. O.; Dean, G. E.; Gimble, F. S.; Jack, W. E.; Neff, N.; Noren, C. J.; Thorner, J.; Belfort, M. (1994) Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature. Nucleic Acids Res 22(7): 1125-7. PubMed ID: 8165123
904.  (INT) (NEB) (REV) Perler, F. B.; Kumar, S.; Kong, H. (1996) Thermostable DNA polymerases. Adv Protein Chem 48: 377-435. PubMed ID: 8791630
905.  (INT) (NEB) Perler, F. B.; Olsen, G. J.; Adam, E. (1997) Compilation and analysis of intein sequences. Nucleic Acids Res 25(6): 1087-93. PubMed ID: 9092614
906.  (APP) (NEB) (REV) Perler, F. B.; Xu, M. Q.; Paulus, H. (1997B) Protein splicing and autoproteolysis mechanisms. Curr Opin Chem Biol 1(3): 292-9. PubMed ID: 9667864
907.  (INT) (NEB) (REV) (Structure) Perler, F. B. (1998) Protein splicing of inteins and hedgehog autoproteolysis: structure, function, and evolution. Cell 92(1): 1-4. PubMed ID: 9489693
908.  (NEB) (REL) (REV) Perler, F. B. (1998B) Breaking up is easy with esters. Nat Struct Biol 5(4): 249-52. PubMed ID: 9546209
909.  (INT) (NEB) Perler, F. B. (1998C) InBase: The NEB Intein Database. The NEB Transcript 9(1): 12.
910.  (INT) (NEB) Perler, F. B. (1999) InBase, the New England Biolabs Intein Database. Nucleic Acids Res 27(1): 346-7. PubMed ID: 9847224
911.  (NEB) (REV) (TS) Perler, F. B. (1999B) A natural example of protein trans-splicing. Trends Biochem Sci 24(6): 209-11. PubMed ID: 10366843
912.  (INT) (NEB) (REV) Perler, F. B.; Xu, M.; Noren, C. J. (1999C) Protein Splicing. In: The Encyclopedia of Molecular Biology, ed. Creighton, T E. (John Wiley and Sons, New York) Vol. 3 pages 2011-2016.
913.  (INT) (NEB) Perler, F. B. (2000) InBase, the Intein Database. Nucleic Acids Res 28(1): 344-5. PubMed ID: 10592269
914.  (APP) (NEB) (REV) Perler, F. B.; Adam, E. (2000B) Protein splicing and its applications. Curr Opin Biotechnol 11(4): 377-83. PubMed ID: 10975457
915.  (INT) (NEB) (REV) Perler, F. B. (2001) Hyperthermophilic inteins. Methods Enzymol 334: 270-80. PubMed ID: 11398469
916.  (INT) (NEB) (REV) Perler, F. B. (2002) InBase: the Intein Database. Nucleic Acids Res 30(1): 383-4. PubMed ID: 11752343
917.  (INT) (NEB) (REV) Perler, Francine B. ; Chen, Lixin; Southworth, Maurice W. (2002B) The mechanism of protein splicing: variations on a theme. In: Peptides 2002: Proceedings of the Twenty-Seventh European Peptide Symposium, ed. Benedetti, E. ; Pedone, C.. (Edizioni Ziino, Napoli, Italy) pages 254-255.
918.  (CPS) (NEB) (REV) Perler, F. B. (2004) The ins and outs of gene expression control. Nat Biotechnol 22(7): 824-6. PubMed ID: 15229542
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940.  (APP) (EPL) (PUR) (REV) Platis, D.; Labrou, N. E. (2008) Chemical and genetic engineering strategies to improve the potency of pharmaceutical proteins and enzymes. Current Medicinal Chemistry 15(19): 1940-1955.
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952.  (APP) (PUR) Prochazkova, K.; Osicka, R.; Linhartova, I.; Halada, P.; Sulc, M.; Sebo, P. (2005) The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC. J Biol Chem 280(5): 3251-8. PubMed ID: 15525636
953.  (APP) (PUR) Qi, Z.; Hamza, I.; O'Brian, M. R. (1999) Heme is an effector molecule for iron-dependent degradation of the bacterial iron response regulator (Irr) protein. Proc Natl Acad Sci U S A 96(23): 13056-61. PubMed ID: 10557272
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960.  (APP) (PUR) (Structure) Rak, A.; Pylypenko, O.; Durek, T.; Watzke, A.; Kushnir, S.; Brunsveld, L.; Waldmann, H.; Goody, R. S.; Alexandrov, K. (2003) Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase. Science 302(5645): 646-50. PubMed ID: 14576435
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967.  (INT) Reith, M.E.; Munholland, J. (1995) Complete nucleotide seuence of the ; Porphyra purpurea; chloroplast genome.. Plant Mol. Biol. Rep. 13: 333-335.
968.  (APP) (CYCL) (NMR) Reitinger, S.; Yu, Y.; Wicki, J.; Ludwiczek, M.; D'Angelo, I.; Baturin, S.; Okon, M.; Strynadka, N. C.; Lutz, S.; Withers, S. G.; McIntosh, L. P. (2010) Circular permutation of Bacillus circulans xylanase: a kinetic and structural study. Biochemistry 49(11): 2464-74. PubMed ID: 20163191
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1016.  (APP) (PUR) Schrader, N.; Kim, E. Y.; Winking, J.; Paulukat, J.; Schindelin, H.; Schwarz, G. (2004) Biochemical characterization of the high affinity binding between the glycine receptor and gephyrin. J Biol Chem 279(18): 18733-41. PubMed ID: 14976213
1017.  (INT) Schufreider, Ann K.; O'Brien, Kathryn M.; McGill, Melissa A.; Reitter, Julie N.; Mills, Kenneth V. (2009) Protein splicing of the Pyrococcus abyssi lon protease intein. FASEB J._MeetingAbstracts 23(1_MeetingAbstracts): 502.2.
1018.  (REL) Schuler, B.; Pannell, L. K. (2002) Specific labeling of polypeptides at amino-terminal cysteine residues using Cy5-benzyl thioester. Bioconjug Chem. 13(5): 1039-43. PubMed ID: 12236786
1019.  (APP) (EPL) Schumacher, M. C.; Resenberger, U.; Seidel, R. P.; Becker, C. F.; Winklhofer, K. F.; Oesterhelt, D.; Tatzelt, J.; Engelhard, M. (2010) Synthesis of a GPI anchor module suitable for protein post-translational modification. Biopolymers 94(4): 457-64. PubMed ID: 20593476
1020.  (APP) (INT) (REV) Schwartz, E. C.; Muir, T. W.; Tyszkiewicz, A. B. (2003) "The splice is right": how protein splicing is opening new doors in protein science. Chem Commun (Camb) (17): 2087-90. PubMed ID: 13678154
1021.  (APP) (CPS) (TS) Schwartz, E. C.; Saez, L.; Young, M. W.; Muir, T. W. (2007) Post-translational enzyme activation in an animal via optimized conditional protein splicing. Nat Chem Biol 3(1): 50-54. PubMed ID: 17128262
1022.  (APP) (PUR) Schwartz, E. C.; Shekhtman, A.; Dutta, K.; Pratt, M. R.; Cowburn, D.; Darst, S.; Muir, T. W. (2008) A full-length group 1 bacterial sigma factor adopts a compact structure incompatible with DNA binding. Chem Biol 15(10): 1091-103. PubMed ID: 18940669
1023.  (APP) (EPL) (REV) Schwarzer, D.; Cole, P. A. (2005) Protein semisynthesis and expressed protein ligation: chasing a protein's tail. Curr Opin Chem Biol 9(6): 561-9. PubMed ID: 16226484
1024.  (APP) (EPL) Schwarzer, D.; Zhang, Z.; Zheng, W.; Cole, P. A. (2006) Negative regulation of a protein tyrosine phosphatase by tyrosine phosphorylation. J Am Chem Soc 128(13): 4192-3. PubMed ID: 16568970
1025.  (APP) (CYCL) Scott, C. P.; Abel-Santos, E.; Wall, M.; Wahnon, D. C.; Benkovic, S. J. (1999) Production of cyclic peptides and proteins in vivo. Proc Natl Acad Sci U S A 96(24): 13638-43. PubMed ID: 10570125
1026.  (APP) (CYCL) Scott, C. P.; Abel-Santos, E.; Jones, A. D.; Benkovic, S. J. (2001) Structural requirements for the biosynthesis of backbone cyclic peptide libraries. Chem Biol 8(8): 801-15. PubMed ID: 11514229
1027.  (APP) (REV) Sears, P.; Tolbert, T.; Wong, C. H. (2001) Enzymatic approaches to glycoprotein synthesis. Genet Eng (N Y) 23: 45-68. PubMed ID: 11570106
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1033.  (APP) (EPL) Seyedsayamdost, M. R.; Yee, C. S.; Reece, S. Y.; Nocera, D. G.; Stubbe, J. (2006) pH Rate profiles of FnY356-R2s (n = 2, 3, 4) in Escherichia coli ribonucleotide reductase: evidence that Y356 is a redox-active amino acid along the radical propagation pathway. J Am Chem Soc 128(5): 1562-8. PubMed ID: 16448127
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1043.  (APP) (INT) (NEB) (PUR) Sharma, S.; Zhang, A.; Wang, H.; Harcum, S. W.; Chong, S. (2003) Study of protein splicing and intein-mediated peptide bond cleavage under high-cell-density conditions. Biotechnol Prog 19(3): 1085-90. PubMed ID: 12790686
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1069.  (APP) (CYCL) (TS) Siebold, C.; Erni, B. (2002) Intein-mediated cyclization of a soluble and a membrane protein in vivo: function and stability. Biophys Chem 96(2-3): 163-71. PubMed ID: 12034438
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1074.  (INT) (REL) (REV) Singh, R.; Robida, M. D.; Karimpour, S. (2006) Building biological complexity with limited genes. Current Genomics 7(2): 97-114.
1075.  (APP) (PUR) Singh, P. K.; Gupta, M. N. (2008) Simultaneous refolding and purification of a recombinant lipase with an intein tag by affinity precipitation with chitosan. Biochim Biophys Acta 1784: 1825-1829. PubMed ID: 18725331
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1080.  (APP) (REL) Skretas, Georgios; Wood, David W. (2005) A Bacterial Biosensor of Endocrine Modulators. Journal of Molecular Biology 349(3): 464-474.
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1108.  (REV) Starokadomskyy, P.L. (2007) Protein Splicing. Molecular Biology 41(2): 278-293.
1109.  (REV) Starokadomskyy, P.L. (2007B) [Protein splicing]. Mol Biol (Mosk) 41(2): 314-30. PubMed ID: 17514899
1110.  (APP) (PUR) Starokadomskyy, P.L.; Dubey, I.Ya.; Okunev, O.V.; Irodov, D. (2007C) [Intein-containing chimeric protein construction and evaluation of its cleavage]. Cytology and Genetics 41(2): 3-11.
1111.  (APP) (PUR) Starokadomskyy, P.L.; Dubey, I.Ya.; Okunev, O.V.; Irodov, D. (2007D) [Intein-containing chimeric protein construction and evaluation of its cleavage conditions]. Tsitol Genet 41(2): 3-11. PubMed ID: 17494337
1112.  (APP) (PUR) Starokadomskyy, P. L.; Okunev, O. V.; Irodov, D. M.; Kordium, V. A. (2008) Utilization of protein splicing for purification of the human growth hormone. Molecular Biology 42(6): 966-972.
1113.  (APP) (PUR) Starokadomskyy, P. L.; Okunev, O. V.; Irodov, D. M.; Kordium, V. A. (2008B) Utilizing of protein splicing for human growth hormone purification. Molekuliarnaia biologiia 42(6): 1085-1092.
1114.  (INT) (REV) Starokadomskyy, P. L. (2010) Protein splicing and its evolution in eukaryotes. Biopolymers and Cell 26(1): 2-12.
1115.  (APP) (PUR) Stasser, J. P.; Siluvai, G. S.; Barry, A. N.; Blackburn, N. J. (2007) A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase. Biochemistry 46(42): 11845-56. PubMed ID: 17902702
1116.  (APP) (NMR) (REV) (Structure) Staunton, D.; Owen, J.; Campbell, I. D. (2003) NMR and structural genomics. Acc Chem Res. 36(3): 207-14. PubMed ID: 12641478
1117.  (APP) (PUR) Stefanidakis, M.; Bjorklund, M.; Ihanus, E.; Gahmberg, C. G.; Koivunen, E. (2003) Identification of a negatively charged peptide motif within the catalytic domain of progelatinases that mediates binding to leukocyte beta 2 integrins. J Biol Chem 278(36): 34674-84. PubMed ID: 12824186
1118.  (APP) (REL) (REV) Stennicke, H. R.; Salvesen, G. S. (2006) Chemical ligation--an unusual paradigm in protease inhibition. Mol Cell 21(6): 727-8. PubMed ID: 16543139
1119.  (APP) (PUR) Stepanenko, V. N.; Esipov, R. S.; Gurevich, A. I.; Chupova, L. A.; Miroshnikov, A. I. (2007B) Recombinant oxyntomodulin. Russian Journal of Bioorganic Chemistry 33(2): 227-232.
1120.  (APP) (PUR) Stepannenko, V. N.; Esipov, R. S.; Gurevich, A. I.; Chupova, L. A.; Miroshnikov, A. I. (2007) [Recombinant oxyntomodulin]. Bioorg Khim 33(2): 245-50. PubMed ID: 17476985
1121.  (REL) Stephenson, R. C.; Clarke, S. (1989) Succinimide formation from aspartyl and asparaginyl peptides as a model for the spontaneous degradation of proteins. J Biol Chem 264(11): 6164-70. PubMed ID: 2703484
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1124.  (EN) (REV) (Structure) Stoddard, B. L. (2006) Homing endonuclease structure and function. Q Rev Biophys 38(1): 49-95. PubMed ID: 16336743
1125.  (APP) (PUR) Stray, J. E.; Lindsley, J. E. (2003) Biochemical analysis of the yeast condensin Smc2/4 complex: an ATPase that promotes knotting of circular DNA. J Biol Chem 278(28): 26238-48. PubMed ID: 12719426
1126.  (APP) (PUR) (Structure) Stray, J. E.; Crisona, N. J.; Belotserkovskii, B. P.; Lindsley, J. E.; Cozzarelli, N. R. (2005) The Saccharomyces cerevisiae Smc2/4 condensin compacts DNA into (+) chiral structures without net supercoiling. J Biol Chem 280(41): 34723-34. PubMed ID: 16100111
1127.  (APP) (EPL) (REV) Strobel, S. A.; Muir, T. W. (2005) Biopolymers: Exploring biological function through chemical manipulation of biopolymers. Current Opinion in Chemical Biology 9(6): 545-547.
1128.  (APP) (PUR) Suarez, M.; Entenza, J. M.; Doerries, C.; Meyer, E.; Bourquin, L.; Sutherland, J.; Marison, I.; Moreillon, P.; Mermod, N. (2003) Expression of a plant-derived peptide harboring water-cleaning and antimicrobial activities. Biotechnol Bioeng 81(1): 13-20. PubMed ID: 12432576
1129.  (NEB) (TS) Sun, L.; Ghosh, I.; Paulus, H.; Xu, M. Q. (2001) Protein trans-splicing to produce herbicide-resistant acetolactate synthase. Appl Environ Microbiol 67(3): 1025-9. PubMed ID: 11229887
1130.  (APP) (EPL) (NEB) (TAG) Sun, L.; Ghosh, I.; Xu, M. Q. (2003) Generation of an affinity column for antibody purification by intein-mediated protein ligation. J Immunol Methods 282(1-2): 45-52. PubMed ID: 14604539
1131.  (APP) (TS) Sun, W.; Yang, J.; Liu, X. Q. (2004) Synthetic two-piece and three-piece split inteins for protein trans-splicing. J Biol Chem 279(34): 35281-6. PubMed ID: 15194682
1132.  (APP) (EPL) (NEB) (TAG) Sun, L.; Rush, J.; Ghosh, I.; Maunus, J. R.; Xu, M. Q. (2004B) Producing peptide arrays for epitope mapping by intein-mediated protein ligation. Biotechniques 37(3): 430-6, 438, 440 passim. PubMed ID: 15470898
1133.  (INT) (NEB) (Structure) Sun, P.; Ye, S.; Ferrandon, S.; Evans, T. C.; Xu, M. Q.; Rao, Z. (2005) Crystal structures of an intein from the split dnaE gene of Synechocystis sp. PCC6803 reveal the catalytic model without the penultimate histidine and the mechanism of zinc ion inhibition of protein splicing. J Mol Biol 353(5): 1093-105. PubMed ID: 16219320
1134.  (APP) (PUR) Sun, Z.; Chen, J.; Yao, H.; Liu, L.; Wang, J.; Zhang, J.; Liu, J. N. (2005) Use of Ssp dnaB derived mini-intein as a fusion partner for production of recombinant human brain natriuretic peptide in Escherichia coli. Protein Expr Purif 43(1): 26-32. PubMed ID: 15979896
1135.  (APP) (EPL) (NEB) (TAG) Sun, L.; Ghosh, I.; Barshevsky, T.; Kochinyan, S.; Xu, M. Q. (2007) Design, preparation and use of ligated phosphoproteins: A novel approach to study protein phosphatases by dot blot array, ELISA and Western blot assays. Methods 42(3): 220-6. PubMed ID: 17532508
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1138.  (APP) (EPL) Sydor, J. R.; Mariano, M.; Sideris, S.; Nock, S. (2002) Establishment of intein-mediated protein ligation under denaturing conditions: C-terminal labeling of a single-chain antibody for biochip screening. Bioconjug Chem 13(4): 707-12. PubMed ID: 12121124
1139.  (APP) (EPL) (TAG) Szewczuk, L. M.; Tarrant, M. K.; Sample, V.; Drury, W. J., 3rd; Zhang, J.; Cole, P. A. (2008) Analysis of serotonin N-acetyltransferase regulation in vitro and in live cells using protein semisynthesis. Biochemistry 47(39): 10407-19. PubMed ID: 18771288
1140.  (APP) (EPL) (REV) (TAG) Szewczuk, L. M.; Tarrant, M. K.; Cole, P. A. (2009) Protein phosphorylation by semisynthesis: from paper to practice. Methods Enzymol 462: 1-24. PubMed ID: 19632467
1141.  (APP) (PUR) Szweda, P.; Pladzyk, R.; Kotlowski, R.; Kur, J. (2001) Cloning, expression, and purification of the Staphylococcus simulans lysostaphin using the intein-chitin-binding domain (CBD) system. Protein Expr Purif 22(3): 467-71. PubMed ID: 11483010
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1144.  (APP) (CYCL) (REL) Takahashi, H.; Arai, M.; Takenawa, T.; Sota, H.; Xie, Q. H.; Iwakura, M. (2007) Stabilization of hyperactive dihydrofolate reductase by cyanocysteine-mediated backbone cyclization. J Biol Chem 282(13): 9420-9. PubMed ID: 17264073
1145.  (APP) (EPL) (TAG) Takeda, S.; Tsukiji, S.; Nagamune, T. (2004) Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation. Bioorg Med Chem Lett 14(10): 2407-10. PubMed ID: 15109622
1146.  (APP) (REL) Takeda, Shuji; Tsukiji, Shinya; Nagamune, Teruyuki (2005) A cysteine-appended deoxyuridine for the postsynthetic DNA modification using native chemical ligation. Tetrahedron Letters 46(13): 2235-2238.
1147.  (APP) (REV) Takeuchi, M.; Ozawa, T. (2006) Frontiers in bioimaging. ACS Chem Biol 1(6): 333-4. PubMed ID: 17163765
1148.  (APP) (PUR) (REV) (TAG) Takeuchi, M.; Ozawa, T. (2007) Methods for imaging and analyses of intracellular organelles using fluorescent and luminescent proteins. Anal Sci 23(1): 25-9. PubMed ID: 17213619
1149.  (REL) Tam, J. P.; Yu, Q. (1998) Methionine ligation strategy in the biomimetic synthesis of parathyroid hormones. Biopolymers 46(5): 319-27. PubMed ID: 9754028
1150.  (EPL) (REV) Tam, J. P. (1999) Ligation and splicing of peptides and proteins. Biopolymers 51(5): 309-10. PubMed ID: 10685042
1151.  (APP) (EPL) (REL) Tam, J. P.; Yu, Q.; Miao, Z. (1999B) Orthogonal ligation strategies for peptide and protein. Biopolymers. 51(5): 311-32. PubMed ID: 10685043
1152.  (APP) (REL) Tam, J. P.; Yu, Q.; Yang, J. L. (2001) Tandem ligation of unprotected peptides through thiaprolyl and cysteinyl bonds in water. J Am Chem Soc. 123(11): 2487-94. PubMed ID: 11456916
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1154.  (APP) (EPL) (REL) Tam, A.; Soellner, M. B.; Raines, R. T. (2007) Water-soluble phosphinothiols for traceless staudinger ligation and integration with expressed protein ligation. J Am Chem Soc 129(37): 11421-30. PubMed ID: 17713909
1155.  (APP) (EPL) Tan, Y.; Ruan, H.; Demeter, M. R.; Comb, M. J. (1999) p90(RSK) blocks bad-mediated cell death via a protein kinase C-dependent pathway. J Biol Chem 274(49): 34859-67. PubMed ID: 10574959
1156.  (APP) (EPL) (TAG) Tan, L. P.; Lue, R. Y.; Chen, G. Y.; Yao, S. Q. (2004) Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo. Bioorg Med Chem Lett 14(24): 6067-70. PubMed ID: 15546731
1157.  (APP) (PUR) (TAG) Tan, L. P.; Chen, G. Y.; Yao, S. Q. (2004B) Expanding the scope of site-specific protein biotinylation strategies using small molecules. Bioorg Med Chem Lett 14(23): 5735-8. PubMed ID: 15501031
1158.  (APP) (REV) (TAG) Tan, L. P.; Yao, S. Q. (2005) Intein-mediated, in vitro and in vivo protein modifications with small molecules. Protein Pept Lett 12(8): 769-75. PubMed ID: 16305547
1159.  (APP) (CPS) Tan, G.; Chen, M.; Foote, C.; Tan, C. (2009) Temperature-sensitive mutations made easy: generating conditional mutations by using temperature-sensitive inteins that function within different temperature ranges. Genetics 183(1): 13-22. PubMed ID: 19596904
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1162.  (APP) (CYCL) Tavassoli, A.; Benkovic, S. J. (2005) Genetically selected cyclic-peptide inhibitors of AICAR transformylase homodimerization. Angew Chem Int Ed Engl 44(18): 2760-3. PubMed ID: 15830403
1163.  (APP) (CYCL) (REV) Tavassoli, A.; Naumann, T.A.; Benkovic, S.J. (2005B) Production of Cyclic Proteins and Peptides. In: Homing Endonucleases and Inteins, ed. Belfort, M.; Derbyshire, V.; Stoddard, B.L.; Wood, D.W.. (Springer-Verlag, Heidelberg, Germany) Vol. 16 pages 293-306.
1164.  (APP) (CYCL) (REV) Tavassoli, A.; Benkovic, S. J. (2007) Split-intein mediated circular ligation used in the synthesis of cyclic peptide libraries in E. coli. Nat Protoc 2(5): 1126-33. PubMed ID: 17546003
1165.  (APP) (CYCL) Tavassoli, A.; Lu, Q.; Gam, J.; Pan, H.; Benkovic, S. J.; Cohen, S. N. (2008) Inhibition of HIV budding by a genetically selected cyclic peptide targeting the Gag-TSG101 interaction. ACS Chem Biol 3(12): 757-64. PubMed ID: 19053244
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1170.  (APP) (EPL) (TAG) Teruya, K.; Nishizawa, K.; Doh-ura, K. (2010) Semisynthesis of a protein with cholesterol at the C-terminal, targeted to the cell membrane of live cells. Protein J 29(7): 493-500. PubMed ID: 20814724
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1176.  (APP) (EPL) Thompson, P. R.; Wang, D.; Wang, L.; Fulco, M.; Pediconi, N.; Zhang, D.; An, W.; Ge, Q.; Roeder, R. G.; Wong, J.; Levrero, M.; Sartorelli, V.; Cotter, R. J.; Cole, P. A. (2004) Regulation of the p300 HAT domain via a novel activation loop. Nat Struct Mol Biol 11(4): 308-15. PubMed ID: 15004546
1177.  (APP) (PUR) Thomson, C. A.; Ananthanarayanan, V. S. (2001) A method for expression and purification of soluble, active Hsp47, a collagen-specific molecular chaperone. Protein Expr Purif 23(1): 8-13. PubMed ID: 11570840
1178.  (APP) (EN) Thomson, J. M.; Lafayette, P. R.; Schmidt, M. A.; Parrott, W. A. (2002) Artificial gene-clusters engineered into plants using a vector system based on intron- and intein-encoded endonucleases. In Vitro Cellular and Developmental Biology - Plant 38(6): 537-542.
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1182.  (APP) (EPL) (TAG) Tolbert, T.J.; Wong, C-H (2000) Intein-Mediated Synthesis of Proteins Containing Carbohydrates and Other Molecular Probes. J Am Chem Soc 122: 5421-5428.
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1190.  (INT) Tyson, G. W.; Chapman, J.; Hugenholtz, P.; Allen, E. E.; Ram, R. J.; Richardson, P. M.; Solovyev, V. V.; Rubin, E. M.; Rokhsar, D. S.; Banfield, J. F. (2004) Community structure and metabolism through reconstruction of microbial genomes from the environment. Nature. 428(6978): 37-43. Epub 2004 Feb 1. PubMed ID: 14961025
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1208.  (APP) (EPL) (TAG) Vila-Perello, M.; Pratt, M. R.; Tulin, F.; Muir, T. W. (2007) Covalent capture of phospho-dependent protein oligomerization by site-specific incorporation of a diazirine photo-cross-linker. J Am Chem Soc 129(26): 8068-9. PubMed ID: 17567014
1209.  (APP) (CPS) (INT) (TS) Vila-Perello, M.; Hori, Y.; Ribo, M.; Muir, T. W. (2008) Activation of protein splicing by protease- or light-triggered O to N acyl migration. Angew Chem Int Ed Engl 47(40): 7764-7. PubMed ID: 18767096
1210.  (APP) (CPS) (CYCL) (EPL) (REV) (TS) Vila-Perello, M.; Muir, T. W. (2010) Biological applications of protein splicing. Cell 143(2): 191-200. PubMed ID: 20946979
1211.  (APP) (EPL) (TAG) Virdee, S.; Ye, Y.; Nguyen, D. P.; Komander, D.; Chin, J. W. (2010) Engineered diubiquitin synthesis reveals Lys29-isopeptide specificity of an OTU deubiquitinase. Nat Chem Biol 6(10): 750-7. PubMed ID: 20802491
1212.  (APP) (EPL) (NMR) (PUR) (Structure) Vitali, F.; Henning, A.; Oberstrass, F. C.; Hargous, Y.; Auweter, S. D.; Erat, M.; Allain, F. H. (2006) Structure of the two most C-terminal RNA recognition motifs of PTB using segmental isotope labeling. EMBO J 25(1): 150-62. PubMed ID: 16362043
1213.  (REV) (TS) Vogel, G. (1998) A two-piece protein assembles itself. Science 281(5378): 763-4. PubMed ID: 9714677
1214.  (APP) (CYCL) (PUR) (TS) Volkmann, Gerrit; Murphy, Peter W.; Rowland, Elden E.; Liu, Xiang-Qin; Byers, David M. (2009) Intein-mediated creation of a cyclic acyl carrier protein. FASEB J._MeetingAbstracts 23(1_MeetingAbstracts): 850.8-.
1215.  (APP) (TAG) (TS) Volkmann, Gerrit; Liu, Xiang-Qin (2009B) C-terminal labelling of recombinant proteins using an engineered split-intein. FASEB J._MeetingAbstracts 23(1_MeetingAbstracts): 863.7.
1216.  (APP) (TS) Volkmann, G.; Sun, W.; Liu, X. Q. (2009C) Controllable protein cleavages through intein fragment complementation. Protein Sci 18(11): 2393-402. PubMed ID: 19768808
1217.  (APP) (TAG) (TS) Volkmann, G.; Liu, X. Q. (2009D) Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein. PLoS One 4(12): e8381. PubMed ID: 20027230
1218.  (APP) (CYCL) (PUR) Volkmann, G.; Murphy, P. W.; Rowland, E. E.; Cronan, J. E., Jr.; Liu, X. Q.; Blouin, C.; Byers, D. M. (2010) Intein-mediated cyclization of bacterial acyl carrier protein stabilizes its folded conformation but does not abolish function. J Biol Chem 285(12): 8605-14. PubMed ID: 20083605
1219.  (APP) (REV) (Structure) Volkmann, G.; Iwai, H. (2010B) Protein trans-splicing and its use in structural biology: opportunities and limitations. Mol Biosyst 6(11): 2110-21. PubMed ID: 20820635
1220.  (APP) (EPL) von Eggelkraut-Gottanka, R.; Machova, Z.; Grouzmann, E.; Beck-Sickinger, A. G. (2003) Semisynthesis and characterization of the first analogues of pro-neuropeptide Y. Chembiochem 4(5): 425-33. PubMed ID: 12740814
1221.  (APP) (EPL) von Eggelkraut-Gottanka, R.; Beck-Sickinger, A. G. (2004) Biosynthesis of peptide hormones derived from precursor sequences. Curr Med Chem 11(20): 2651-65. PubMed ID: 15544467
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1224.  (APP) (PUR) (Structure) Wahl, M. C.; Huber, R.; Prade, L.; Marinkovic, S.; Messerschmidt, A.; Clausen, T. (1997) Cloning, purification, crystallization, and preliminary X-ray diffraction analysis of cystathionine gamma-synthase from E. coli. FEBS Lett 414(3): 492-6. PubMed ID: 9323022
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1228.  (APP) (EPL) (NMR) (PUR) (Structure) Walters, K. J.; Lech, P. J.; Goh, A. M.; Wang, Q.; Howley, P. M. (2003) DNA-repair protein hHR23a alters its protein structure upon binding proteasomal subunit S5a. Proc Natl Acad Sci U S A 100(22): 12694-9. PubMed ID: 14557549
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1230.  (APP) (EPL) Wang, D.; Cole, P. A. (2001) Protein tyrosine kinase Csk-catalyzed phosphorylation of Src containing unnatural tyrosine analogues. J Am Chem Soc 123(37): 8883-6. PubMed ID: 11552794
1231.  (APP) (NEB) (PUR) (TAG) Wang, H.; Chong, S. (2003) Visualization of coupled protein folding and binding in bacteria and purification of the heterodimeric complex. Proc Natl Acad Sci U S A. 100(2): 478-83. Epub 2003 Jan 6. PubMed ID: 12515863
1232.  (APP) (PUR) Wang, Z. C.; Teng, Z. P.; Zhang, X. G.; Guo, X. C.; Yuan, J. M.; Zeng, Y. (2003) Obtaining purified p24 gag protein of HIV-1 by using a new prokaryotic expressive vector. Chinese Journal of Microbiology and Immunology 23(5): 375-379.
1233.  (APP) (REV) (TAG) Wang, H.; Chen, X. (2008) Site-specifically modified fusion proteins for molecular imaging. Front Biosci 13: 1716-32. PubMed ID: 17981663
1234.  (APP) (PUR) (TAG) Wang, Z.; Wu, H.; Chen, J.; Zhang, J.; Yao, Y.; Chen, G. Q. (2008B) A novel self-cleaving phasin tag for purification of recombinant proteins based on hydrophobic polyhydroxyalkanoate nanoparticles. Lab Chip 8(11): 1957-62. PubMed ID: 18941699
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1236.  (APP) (PUR) Wang, P. Z.; Gu, J. B.; Luo, J.; Peng, H. J. (2010B) [Antibacterial activity of recombinant thanatin expressed by E.coli ER2566.]. Nan Fang Yi Ke Da Xue Xue Bao 30(5): 1050-4. PubMed ID: 20501392
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1292.  (APP) (REL) Xie, L.; Miller, L. M.; Chatterjee, C.; Averin, O.; Kelleher, N. L.; van der Donk, W. A. (2004) Lacticin 481: in vitro reconstitution of lantibiotic synthetase activity. Science. 303(5658): 679-81. PubMed ID: 14752162
1293.  (APP) (EPL) (TAG) Xie, N.; Elangwe, E. N.; Asher, S.; Zheng, Y. G. (2009) A dual-mode fluorescence strategy for screening HAT modulators. Bioconjug Chem 20(2): 360-6. PubMed ID: 19146394
1294.  (INT) (NEB) Xu, M. Q.; Southworth, M. W.; Mersha, F. B.; Hornstra, L. J.; Perler, F. B. (1993) In vitro protein splicing of purified precursor and the identification of a branched intermediate. Cell 75(7): 1371-7. PubMed ID: 8269515
1295.  (INT) (NEB) Xu, M. Q.; Comb, D. G.; Paulus, H.; Noren, C. J.; Shao, Y.; Perler, F. B. (1994) Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation. Embo J 13(23): 5517-22. PubMed ID: 7988548
1296.  (INT) (NEB) Xu, M. Q.; Perler, F. B. (1996) The mechanism of protein splicing and its modulation by mutation. Embo J 15(19): 5146-53. PubMed ID: 8895558
1297.  (APP) (NEB) (PUR) (REV) Xu, M.-Q. (1997) The IMPACT of protein splicing research. The NEB Transcript 8(2): 1-5.
1298.  (APP) (EPL) (NMR) (PUR) (Structure) Xu, R.; Ayers, B.; Cowburn, D.; Muir, T. W. (1999) Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies. Proc Natl Acad Sci U S A. 96(2): 388-93. PubMed ID: 9892643
1299.  (NEB) (REL) (REV) Xu, Q.; Buckley, D.; Guan, C.; Guo, H. C. (1999B) Structural insights into the mechanism of intramolecular proteolysis. Cell. 98(5): 651-61. PubMed ID: 10490104
1300.  (APP) (NEB) (PUR) (REV) Xu, M. Q.; Paulus, H.; Chong, S. (2000) Fusions to self-splicing inteins for protein purification. Methods Enzymol 326: 376-418. PubMed ID: 11036654
1301.  (APP) (EPL) (NEB) (REV) Xu, M. Q.; Evans, T. C., Jr. (2001) Intein-mediated ligation and cyclization of expressed proteins. Methods 24(3): 257-77. PubMed ID: 11403575
1302.  (APP) (NEB) (PUR) (REV) Xu, M. Q.; Evans, T. C., Jr. (2003) Purification of recombinant proteins from E. coli by engineered inteins. Methods Mol Biol 205: 43-68. PubMed ID: 12491879
1303.  (APP) (EPL) (NEB) (TAG) Xu, J.; Sun, L.; Ghosh, I.; Xu, M. Q. (2004) Western blot analysis of Src kinase assays using peptide substrates ligated to a carrier protein. Biotechniques 36(6): 976-8, 980-1. PubMed ID: 15211748
1304.  (APP) (NEB) (REV) Xu, M. Q.; Evans, T. C., Jr. (2005) Recent advances in protein splicing: manipulating proteins in vitro and in vivo. Curr Opin Biotechnol 16(4): 440-6. PubMed ID: 16026977
1305.  (APP) (EPL) (NEB) (TAG) Xu, M. Q.; Ghosh, I.; Kochinyan, S.; Sun, L. (2007) Intein-mediated peptide arrays for epitope mapping and kinase/phosphatase assays. Methods Mol Biol 381: 313-38. PubMed ID: 17984527
1306.  (APP) (PUR) Xu, C. G.; Fan, X. J.; Zhang, Z. Y.; Fu, Y. J.; Liang, A. H. (2007) [Soluble expression, purification and characterization of Bm K IT in Escherichia coli by intein-mediated system]. Sheng Wu Gong Cheng Xue Bao 23(6): 989-94. PubMed ID: 18257224
1307.  (APP) (PUR) Xu, C. G.; Fan, X. J.; Fu, Y. J.; Liang, A. H. (2008) Effect of location of the His-tag on the production of soluble and functional Buthus martensii Karsch insect toxin. Protein Expr Purif 59(1): 103-9. PubMed ID: 18289876
1308.  (APP) (NMR) (PUR) (Structure) (TS) Yagi, H.; Tsujimoto, T.; Yamazaki, T.; Yoshida, M.; Akutsu, H. (2004) Conformational change of H+-ATPase beta monomer revealed on segmental isotope labeling NMR spectroscopy. J Am Chem Soc 126(50): 16632-8. PubMed ID: 15600369
1309.  (INT) Yamamoto, K.; Low, B.; Rutherford, S. A.; Rajagopalan, M.; Madiraju, M. V. (2001) The Mycobacterium avium-intracellulare complex dnaB locus and protein intein splicing. Biochem Biophys Res Commun 280(3): 898-903. PubMed ID: 11162608
1310.  (INT) Yamamoto, K.; Rutherford, S. A.; Rajagopalan, M.; Hirakawa, H.; Kuhara, S.; Banno, Y.; Fujii, H.; Madiraju, M. V. V. S. (2004) Heterogeneity of dnaB Locus of Mycobacterium avium-intracellulare Complex. Journal of the Faculty of Agriculture, Kyushu University 49(2): 375-381.
1311.  (APP) (REL) Yamamoto, N.; Takayanagi, A.; Yoshino, A.; Sakakibara, T.; Kajihara, Y. (2007) An approach for a synthesis of asparagine-linked sialylglycopeptides having intact and homogeneous complex-type undecadisialyloligosaccharides. Chemistry 13(2): 613-25. PubMed ID: 16977655
1312.  (APP) (NMR) (Structure) (TS) Yamazaki, T.; Otomo, T.; Oda, N.; Kyogoku, Y.; Uegaki, K.; Ito, N.; Ishino, Y.; Nakamura, H. (1998) Segmental isotope labeling for protein NMR using peptide splicing. J. Am. Chem. Soc. 120: 5591-5592.
1313.  (APP) (PUR) Yan, S. S.; Yan, J.; Shi, G.; Xu, Q.; Chen, S. C.; Tian, Y. W. (2005) Production of native protein by using Synechocystis sp. PCC6803 DnaB mini-intein in Escherichia coli. Protein Expr Purif 40(2): 340-5. PubMed ID: 15766876
1314.  (APP) (TS) Yang, J.; Fox, G. C., Jr.; Henry-Smith, T. V. (2003) Intein-mediated assembly of a functional beta-glucuronidase in transgenic plants. Proc Natl Acad Sci U S A 100(6): 3513-8. PubMed ID: 12629210
1315.  (INT) Yang, J.; Meng, Q.; Liu, X. Q. (2004) Intein harbouring large tandem repeats in replicative DNA helicase of Trichodesmium erythraeum. Mol Microbiol 51(4): 1185-92. PubMed ID: 14763989
1316.  (APP) (PUR) (TS) Yang, J.; Henry-Smith, T. V.; Qi, M. (2006) Functional analysis of the split Synechocystis DnaE intein in plant tissues by biolistic particle bombardment. Transgenic Res 15(5): 583-93. PubMed ID: 16830226
1317.  (APP) (PUR) Yang, B.; Chen, S.; Tong, Y.; Qin, Y. (2006B) [Construction and expression of protein self-splicing prokaryotic expression vector pTWIN1- AcAPc2]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 23(3): 630-4. PubMed ID: 16856404
1318.  (APP) (TAG) (TS) Yang, J. Y.; Yang, W. Y. (2009) Site-specific two-color protein labeling for FRET studies using split inteins. J Am Chem Soc 131(33): 11644-5. PubMed ID: 19645470
1319.  (APP) (PUR) Ye, Xinhao; Rollin, Joe; Zhang, Yi-Heng Percival (2010) Thermophilic [alpha]-glucan phosphorylase from Clostridium thermocellum: Cloning, characterization and enhanced thermostability. Journal of Molecular Catalysis B: Enzymatic 65(1-4): 110-116.
1320.  (APP) (EPL) (TAG) Yee, C. S.; Seyedsayamdost, M. R.; Chang, M. C.; Nocera, D. G.; Stubbe, J. (2003) Generation of the R2 subunit of ribonucleotide reductase by intein chemistry: insertion of 3-nitrotyrosine at residue 356 as a probe of the radical initiation process. Biochemistry 42(49): 14541-52. PubMed ID: 14661967
1321.  (APP) (EPL) Yee, C. S.; Chang, M. C.; Ge, J.; Nocera, D. G.; Stubbe, J. (2003) 2,3-difluorotyrosine at position 356 of ribonucleotide reductase R2: a probe of long-range proton-coupled electron transfer. J Am Chem Soc 125(35): 10506-7. PubMed ID: 12940718
1322.  (APP) (EPL) (REV) Yeo, D. S.; Srinivasan, R.; Chen, G. Y.; Yao, S. Q. (2004) Expanded utility of the native chemical ligation reaction. Chemistry 10(19): 4664-72. PubMed ID: 15372685
1323.  (APP) (EPL) (REV) (TAG) Yeo, D. S. Y.; Panicker, R. C.; Tan, L. P.; Yao, S. Q. (2004) Strategies for immobilization of biomolecules in a microarray. Combinatorial Chemistry and High Throughput Screening 7(3): 213-221.
1324.  (APP) (PUR) Yi, T.; Hong, A.; Xie, S.; Zhang, L.; Xie, Q.; Dai, Y.; Yu, R. (2008) [Preparation and identification of recombinant maxadilan]. Sheng Wu Gong Cheng Xue Bao 24(12): 2049-55.
1325.  (INT) York, Daniel J.; Nadelson, Adam C.; Reitter, Julie N.; Mills, Kenneth V. (2009) Alternate protein splicing mechanisms: A directed evolution approach. FASEB J._MeetingAbstracts 23(1_MeetingAbstracts): 502.4.
1326.  (INT) York, Daniel J.; Duffee, Lauren R.; Dorval, Deirdre M.; Reitter, Julie N.; Mills, Kenneth V. (2010) Estimating the activation barrier to each step of protein splicing for the non-canonical P. abyssi PolII intein. FASEB J. 24(1_MeetingAbstracts): 463.18-.
1327.  (APP) (NMR) (REV) (Structure) Yu, H. (1999) Extending the size limit of protein nuclear magnetic resonance. Proc Natl Acad Sci U S A 96(2): 332-4. PubMed ID: 9892632
1328.  (APP) (PUR) Yu, R. J.; Hong, A.; Dai, Y.; Gao, Y. (2004) Intein-mediated rapid purification of recombinant human pituitary adenylate cyclase activating polypeptide. Acta Biochim Biophys Sin (Shanghai) 36(11): 759-66. PubMed ID: 15514850
1329.  (APP) (PUR) Yu, R. J.; Xie, Q. L.; Dai, Y.; Gao, Y.; Zhou, T. H.; Hong, A. (2006) Intein-mediated rapid purification and characterization of a novel recombinant agonist for VPAC2. Peptides 27(6): 1359-66. PubMed ID: 16500728
1330.  (APP) (PUR) Yu, R. J.; Tam, N. L.; Gao, Y.; Zeng, Z. H.; Zhou, T. H.; Hong, A. (2007) A novel recombinant, VPAC2-selective agonist enhancing insulin release and glucose disposal. Acta Pharmacol Sin 28(4): 526-33. PubMed ID: 17376292
1331.  (APP) (PUR) Yu, R.; Yi, T.; Zhang, L.; Hong, A.; Dai, Y.; Zhou, T. (2008) Intein-mediated rapid purification of recombinant maxadilan and M65 and their acute effects on plasma glucose. Acta Biochim Biophys Sin (Shanghai) 40(12): 1015-22. PubMed ID: 19089299
1332.  (APP) (EPL) Yu, H. H.; Nakase, I.; Pujals, S.; Hirose, H.; Tanaka, G.; Katayama, S.; Imanishi, M.; Futaki, S. (2010) Expressed protein ligation for the preparation of fusion proteins with cell penetrating peptides for endotoxin removal and intracellular delivery. Biochim Biophys Acta (IN PRESS). PubMed ID: 20170629
1333.  (APP) (CPS) Yuen, C. M.; Rodda, S. J.; Vokes, S. A.; McMahon, A. P.; Liu, D. R. (2006) Control of transcription factor activity and osteoblast differentiation in Mammalian cells using an evolved small-molecule-dependent intein. J Am Chem Soc 128(27): 8939-46. PubMed ID: 16819890
1334.  (APP) (CPS) Zeidler, M. P.; Tan, C.; Bellaiche, Y.; Cherry, S.; Hader, S.; Gayko, U.; Perrimon, N. (2004) Temperature-sensitive control of protein activity by conditionally splicing inteins. Nat Biotechnol 22(7): 871-6. PubMed ID: 15184905
1335.  (APP) (PUR) Zeng, L.; Yu, R.; Xu, M.; Chen, J.; Wang, J.; Li, J. (2009) [Preparation and identification of recombinant PTD-maxadilan]. Sheng Wu Gong Cheng Xue Bao 25(11): 1739-45. PubMed ID: 20222476
1336.  (EN) (REL) Zeng, Q.; Bonocora, R. P.; Shub, D. A. (2009) A free-standing homing endonuclease targets an intron insertion site in the psbA gene of cyanophages. Curr Biol 19(3): 218-22. PubMed ID: 19200728
1337.  (TS) Zettler, J.; Schutz, V.; Mootz, H. D. (2009) The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction. FEBS Lett 583(5): 909-14. PubMed ID: 19302791
1338.  (APP) (NEB) (PUR) (TAG) Zhang, A.; Gonzalez, S. M.; Cantor, E. J.; Chong, S. (2001) Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins. Gene 275(2): 241-52. PubMed ID: 11587851
1339.  (APP) (EPL) Zhang, Z.; Shen, K.; Lu, W.; Cole, P. A. (2003) The role of C-terminal tyrosine phosphorylation in the regulation of SHP-1 explored via expressed protein ligation. J Biol Chem 278(7): 4668-74. PubMed ID: 12468540
1340.  (APP) (PUR) Zhang, X. L.; Wang, J. R.; Zhang, Y.; Chen, M. L.; Zhang, W.; Yang, S.; Jiang, W. H. (2003B) [Expression, purification and identification of recombinant SARS coronavirus membrane protein]. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35(12): 1140-4. PubMed ID: 14673508
1341.  (APP) (PUR) Zhang, Y.; Calin-Jageman, I.; Gurnon, J. R.; Choi, T. J.; Adams, B.; Nicholson, A. W.; Van Etten, J. L. (2003C) Characterization of a chlorella virus PBCV-1 encoded ribonuclease III. Virology 317(1): 73-83. PubMed ID: 14675626
1342.  (APP) (PUR) Zhang, X. L.; Wang, J. R.; Zhang, Y.; Chen, M. L.; Zhang, W.; Yang, S.; Jiang, W. H. (2003C) Expression, purification and identification of recombinant SARS coronavirus membrane protein. Acta Biochimica et Biophysica Sinica 35(12): 1140-1144.
1343.  (APP) (NEB) (PUR) Zhang, Aihua; Cantor, Eric J.; Barshevsky, Tanya; Chong, Shaorong (2005) Productive interaction of chaperones with substrate protein domains allows correct folding of the downstream GFP domain. Gene 350(1): 25-31.
1344.  (INT) Zhang, L.; Zheng, Y.; Xi, Z.; Luo, Z.; Xu, X.; Wang, C.; Liu, Y. (2009) Metal ions binding to recA inteins from Mycobacterium tuberculosis. Mol Biosyst 5(6): 644-50. PubMed ID: 19462022
1345.  (APP) (EPL) (REV) Zhang, X.; van der Donk, W. A. (2009B) Using expressed protein ligation to probe the substrate specificity of lantibiotic synthetases. Methods Enzymol 462: 117-34. PubMed ID: 19632472
1346.  (CPS) (INT) Zhang, L.; Xiao, N.; Pan, Y.; Zheng, Y.; Pan, Z.; Luo, Z.; Xu, X.; Liu, Y. (2010) Binding and inhibition of copper ions to RecA inteins from Mycobacterium tuberculosis. Chemistry 16(14): 4297-306. PubMed ID: 20209535
1347.  (APP) (PUR) Zhang, S.; Wang, Z. H.; Chen, G. Q. (2010B) Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification. Microb Cell Fact 9: 28. PubMed ID: 20459707
1348.  (APP) (PUR) (TS) Zhao, J.; Gao, S. Q.; Fei, Y. B.; Wei, L. B. (2004) [Construction and expression of vector with aroA-in gene and its transformation in tobacco]. Yi Chuan Xue Bao 31(11): 1294-301. PubMed ID: 15651683
1349.  (APP) (PUR) (TS) Zhao, J.; Gao, S. Q.; Fei, Y. B.; Wei, L. B. (2004) Construction and expression of vector with aroA-In gene and its transformation in tobacco. Acta Genetica Sinica 31(11): 1294-1301.
1350.  (APP) (NEB) (PUR) Zhao, Z.; Lu, W.; Dun, B.; Jin, D.; Ping, S.; Zhang, W.; Chen, M.; Xu, M. Q.; Lin, M. (2008) Purification of green fluorescent protein using a two-intein system. Appl Microbiol Biotechnol 77(5): 1175-80. PubMed ID: 17973109
1351.  (APP) (EPL) (NMR) (PUR) (Structure) Zhao, W.; Zhang, Y.; Cui, C.; Li, Q.; Wang, J. (2008B) An efficient on-column expressed protein ligation strategy: application to segmental triple labeling of human apolipoprotein E3. Protein Sci 17(4): 736-47. PubMed ID: 18305193
1352.  (APP) (CYCL) (TS) Zhao, Z.; Ma, X.; Li, L.; Zhang, W.; Ping, S.; Xu, M. Q.; Lin, M. (2010) Protein cyclization enhanced thermostability and exopeptidase-resistance of green fluorescent protein. J Microbiol Biotechnol 20(3): 460-6. PubMed ID: 20372012
1353.  (INT) Zheng, R.; Matsui, E.; Shen, Y.; Musti, K. V.; Feng, Y.; Darnis, S.; Kawarabayasi, Y.; Kikuchi, H.; Harata, K.; Matsui, I. (2001) The novel function of a short region K253xRxxxD259 conserved in the exonuclease domain of hyperthermostable DNA polymerase I from Pyrococcus horikoshii. Extremophiles 5(2): 111-7. PubMed ID: 11354454
1354.  (APP) (EPL) Zheng, W.; Schwarzer, D.; Lebeau, A.; Weller, J. L.; Klein, D. C.; Cole, P. A. (2005) Cellular stability of serotonin N-acetyltransferase conferred by phosphonodifluoromethylene alanine (Pfa) substitution for Ser-205. J Biol Chem 280(11): 10462-7. PubMed ID: 15632116
1355.  (APP) (PUR) Zhgun, A. A.; El'darov, M. A.; Solodar, L. I.; Sokolov, N. N.; Archakov, A. I.; Skriabin, K. G. (2001) [Heterologous expression of eukaryotic CYP450. 1. Heterologous expression of cytochrome P450 2B4 using groups with various affinity in E. coli]. Vopr Med Khim 47(4): 382-92. PubMed ID: 11693026
1356.  (APP) (PUR) Zhong, Q.; Lazar, C. S.; Tronchere, H.; Sato, T.; Meerloo, T.; Yeo, M.; Songyang, Z.; Emr, S. D.; Gill, G. N. (2002) Endosomal localization and function of sorting nexin 1. Proc Natl Acad Sci U S A 99(10): 6767-72. PubMed ID: 11997453
1357.  (APP) (CYCL) (TS) Zhonglin, Z.; Ma, X.; Li, L.; Zhang, W.; Ping, S.; Xu, M. Q.; Lin, M. (2010) Protein cyclization enhanced thermostability and exopeptidase resistance of green fluorescent protein. Journal of Microbiology and Biotechnology 20(3): 460-466.
1358.  (APP) (PUR) Zhou, H.; Yan, F.; Tai, H. H. (2001) C-Terminal region of human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase is involved in the interaction with prostaglandin substrates. Eur J Biochem 268(12): 3368-74. PubMed ID: 11422365
1359.  (APP) (PUR) Zhou, X.; Song, Z.; Liu, X.; Jia, F.; Wang, Y. (2010) Production of Recombinant Porcine Interferon alpha Using PHB-Intein-Mediated Protein Purification Strategy. Appl Biochem Biotechnol (IN PRESS). PubMed ID: 20957450
1360.  (INT) Zhu, W.; Reich, C. I.; Olsen, G. J.; Giometti, C. S.; Yates, J. R., 3rd (2004) Shotgun proteomics of Methanococcus jannaschii and insights into methanogenesis. J Proteome Res 3(3): 538-48. PubMed ID: 15253435
1361.  (APP) (PUR) (TS) Zhu, F.; Miao, J.; Qu, H.; Chi, X. (2009) [Separate expression of ABCA1 and ligation mediated by Ssp DnaE intein in Escherichia coli BL21 (DE3)]. Wei Sheng Wu Xue Bao 49(12): 1601-6. PubMed ID: 20222445
1362.  (APP) (PUR) (TS) Zhu, F.; Liu, Z.; Qu, H.; Xin, X.; Dong, H.; Liu, X. (2009B) [Ssp DnaB intein-mediated ligation of heavy and light chains of coagulation factor VIII in Escherichia coli]. Sheng Wu Gong Cheng Xue Bao 25(7): 1101-6. PubMed ID: 19835155
1363.  (APP) (PUR) (TS) Zhu, F. X.; Liu, Z. L.; Qu, H. G.; Chi, X. Y. (2009C) [Intein-mediated F309SfVIII ligation with enhanced secretion of its heavy chain.]. Sheng Li Xue Bao 61(6): 526-32. PubMed ID: 20029685
1364.  (APP) (PUR) (TS) Zhu, F.; Liu, Z.; Chi, X.; Qu, H. (2010) Protein trans-splicing based dual-vector delivery of the coagulation factor VIII gene. Sci China Life Sci 53(6): 683-9. PubMed ID: 20602271
1365.  (APP) (PUR) (TS) Zhu, F. X.; Yang, S. D.; Liu, Z. L.; Miao, J.; Qu, H. G.; Chi, X. Y. (2010B) vWF improves secretion and activity of intein spliced BDD-FVIII. Yaoxue Xuebao 45(5): 595-600.
1366.  (APP) (PUR) (TS) Zhu, F. X.; Liu, Z. L.; Qu, H. G.; Chi, X. Y. (2010C) Post-translational ligation and function of dual-vector transferred split CFTR gene. Yaoxue Xuebao 45(1): 60-65.
1367.  (APP) (TS) Zhu, F. X.; Liu, Z. L.; Qu, H. G.; Chi, X. Y. (2010D) [Enhancing effect of porcine coagulation factor VIII A1 and A3 domains on secretion of post-translationally spliced human/porcine hybrid coagulation factor VIII.]. Sheng Li Xue Bao 62(4): 373-81. PubMed ID: 20717639
1368.  (APP) (EPL) (REL) Ziaco, B.; Pensato, S.; D'Andrea, L. D.; Benedetti, E.; Romanelli, A. (2008) Semisynthesis of dimeric proteins by expressed protein ligation. Org Lett 10(10): 1955-8. PubMed ID: 18410123
1369.  (APP) (EPL) Zuberek, J.; Wyslouch-Cieszynska, A.; Niedzwiecka, A.; Dadlez, M.; Stepinski, J.; Augustyniak, W.; Gingras, A. C.; Zhang, Z.; Burley, S. K.; Sonenberg, N.; Stolarski, R.; Darzynkiewicz, E. (2003) Phosphorylation of eIF4E attenuates its interaction with mRNA 5' cap analogs by electrostatic repulsion: intein-mediated protein ligation strategy to obtain phosphorylated protein. RNA 9(1): 52-61. PubMed ID: 12554876
1370.  (APP) (NMR) (Structure) (TS) Zuger, S.; Iwai, H. (2005) Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies. Nat Biotechnol 23(6): 736-40. PubMed ID: 15908942
1371.  (APP) (PUR) Zzaman, S.; Abhyankar, M. M.; Bastia, D. (2004) Reconstitution of F factor DNA replication in vitro with purified proteins. J Biol Chem 279(17): 17404-10. PubMed ID: 14973139

Bibliography updated: 11/05/10

Last database update: 11/05/10

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