New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
shopping cart | log in
Contact NEB About Us Site Map Request a Catalog OEM at NEB ISO International Orders Freezer Program Quick Order
Home > Technical Reference > InBase > Protein Splicing Bibliography
InBase, The Intein Database: Protein Splicing Bibliography

InBase Reference: Perler, F. B. (2002). InBase, the Intein Database. Nucleic Acids Res. 30, 383-384.

If you think a paper is missing from InBase, please send the reference and PDF (if possible) to the curator at inbase@neb.com.

The following annotations have been used in the InBase Bibliography. Please inform the curator if you notice a missing or incorrect annotation.

Select: Annotation Key:
(APP) Application
(CPS) Conditional Protein Splicing (cis or trans)
(CYCL) Protein/peptide backbone cyclization
(EN) Homing endonuclease
(EPL) IPL, intein-mediated expressed protein ligation
(INT) References describing inteins or sequenced genomes containing inteins
(NEB) Papers from NEB
(NMR) Segmental labeling for NMR or other types of analysis
(PUR) Intein vector systems and examples of intein-mediated purifications
(REL) Related area of interest
(REV) Review, letter, note or commentary
(Structure) Structural papers of inteins or other proteins prepared using intein technology
(TAG) Addition of binding or detection tag
(TS) Trans-splicing
Recent publications are in Red

Sorting order:

1.  (APP) (PUR) Abbing, A.; Blaschke, U. K.; Grein, S.; Kretschmar, M.; Stark, C. M.; Thies, M. J.; Walter, J.; Weigand, M.; Woith, D. C.; Hess, J.; Reiser, C. O. (2004) Efficient intracellular delivery of a protein and a low molecular weight substance via recombinant polyomavirus-like particles. J Biol Chem 279(26): 27410-21. PubMed ID: 15102846
2.  (INT) (REL) (REV) Abdelhaleem, M. (2010) Helicases: an overview. Methods Mol Biol 587: 1-12. PubMed ID: 20225138
3.  (APP) (CYCL) (REV) Abel-Santos, E.; Scott, C. P.; Benkovic, S. J. (2003) Use of inteins for the in vivo production of stable cyclic peptide libraries in E. coli. Methods Mol Biol 205: 281-94. PubMed ID: 12491894
4.  (APP) (PUR) Abhyankar, M. M.; Zzaman, S.; Bastia, D. (2003) Reconstitution of R6K DNA replication in vitro using 22 purified proteins. J Biol Chem 278(46): 45476-84. PubMed ID: 12970346
5.  (APP) (CPS) (INT) (NEB) Adam, E.; Perler, F. B. (2002) Development of a positive genetic selection system for inhibition of protein splicing using mycobacterial inteins in Escherichia coli DNA gyrase subunit A. J Mol Microbiol Biotechnol 4(5): 479-87. PubMed ID: 12432958
6.  (INT) (NEB) (REV) Adams, M. W.; Perler, F. B.; Kelly, R. M. (1995) Extremozymes: expanding the limits of biocatalysis. Biotechnology (N Y) 13(7): 662-8. PubMed ID: 9634802
7.  (REL) (REV) Adamska, M.; Matus, D. Q.; Adamski, M.; Green, K.; Rokhsar, D. S.; Martindale, M. Q.; Degnan, B. M. (2007) The evolutionary origin of hedgehog proteins. Curr Biol 17(19): R836-7. PubMed ID: 17925209
8.  (REL) Agarwal, S. M.; Srivastava, P. K. (2009) Human intronless disease associated genes are slowly evolving. BMB Rep 42(6): 356-60. PubMed ID: 19558794
9.  (APP) (EPL) (TAG) Ajish Kumar, K. S.; Haj-Yahya, M.; Olschewski, D.; Lashuel, H. A.; Brik, A. (2009) Highly efficient and chemoselective peptide ubiquitylation. Angew Chem Int Ed Engl 48(43): 8090-4. PubMed ID: 19780082
10.  (APP) (PUR) (TS) Al-Ali, H.; Ragan, T. J.; Gao, X.; Harris, T. K. (2007) Reconstitution of modular PDK1 functions on trans-splicing of the regulatory PH and catalytic kinase domains. Bioconjug Chem 18(4): 1294-302. PubMed ID: 17500509
11.  (REL) (Structure) Albert, A.; Dhanaraj, V.; Genschel, U.; Khan, G.; Ramjee, M. K.; Pulido, R.; Sibanda, B. L.; von Delft, F.; Witty, M.; Blundell, T. L.; Smith, A. G.; Abell, C. (1998) Crystal structure of aspartate decarboxylase at 2.2 A resolution provides evidence for an ester in protein self-processing. Nat Struct Biol 5(4): 289-93. PubMed ID: 9546220
12.  (EN) (REV) Alexander, Rebecca W. (2002) Measure for measure: a zinc finger ruler. Trends in Biochemical Sciences 27(8): 390.
13.  (APP) (EPL) (TAG) Alexandrov, K.; Heinemann, I.; Durek, T.; Sidorovitch, V.; Goody, R. S.; Waldmann, H. (2002) Intein-mediated synthesis of geranylgeranylated Rab7 protein in vitro. J Am Chem Soc 124(20): 5648-9. PubMed ID: 12010032
14.  (APP) (EPL) (REL) Alves, D. A.; Esser, D.; Broadbridge, R. J.; Beevers, A. P. G.; Chapman, C. P.; Winsor, C. E.; Betley, J. R. (2003) Synthesis and use of a pseudo-cysteine for native chemical ligation. Journal of Peptide Science 9(4): 221-228.
15.  (APP) (REV) Amitai, G.; Pietrokovski, S. (1999) Fine-tuning an engineered intein. Nat Biotechnol 17(9): 854-5. PubMed ID: 10471922
16.  (INT) (REL) Amitai, G.; Belenkiy, O.; Dassa, B.; Shainskaya, A.; Pietrokovski, S. (2003) Distribution and function of new bacterial intein-like protein domains. Mol Microbiol 47(1): 61-73. PubMed ID: 12492854
17.  (INT) Amitai, G.; Dassa, B.; Pietrokovski, S. (2004) Protein splicing of inteins with atypical glutamine and aspartate C-terminal residues. J Biol Chem 279(5): 3121-31. PubMed ID: 14593103
18.  (APP) (INT) Amitai, G.; Callahan, B. P.; Stanger, M. J.; Belfort, G.; Belfort, M. (2009) Modulation of intein activity by its neighboring extein substrates. Proc Natl Acad Sci U S A 106(27): 11005-10. PubMed ID: 19541659
19.  (APP) (EPL) Anderson, L. L.; Marshall, G. R.; Baranski, T. J. (2005) Expressed protein ligation to study protein interactions: semi-synthesis of the G-protein alpha subunit. Protein Pept Lett 12(8): 783-7. PubMed ID: 16305549
20.  (APP) (EPL) Anderson, L. L.; Marshall, G. R.; Crocker, E.; Smith, S. O.; Baranski, T. J. (2005B) Motion of carboxyl terminus of Galpha is restricted upon G protein activation. A solution NMR study using semisynthetic Galpha subunits. J Biol Chem 280(35): 31019-26. PubMed ID: 15983037
21.  (APP) (CPS) (TS) Ando, T.; Tsukiji, S.; Tanaka, T.; Nagamune, T. (2007) Construction of a small-molecule-integrated semisynthetic split intein for in vivo protein ligation. Chem Commun (Camb) (47): 4995-7. PubMed ID: 18049731
22.  (INT) (REV) Anraku, Y.; Hirata, R. (1994) Protozyme: emerging evidence in nature. J Biochem (Tokyo) 115(2): 175-8. PubMed ID: 8206863
23.  (INT) (REV) Anraku, Y. (1997) [The discovery and molecular mechanism of protein splicing]. Tanpakushitsu Kakusan Koso 42(15): 2483-97. PubMed ID: 9391338
24.  (INT) (REV) Anraku, Y. (1997B) Protein splicing: its chemistry and biology. Genes Cells 2(6): 359-67. PubMed ID: 9286854
25.  (INT) (REV) Anraku, Y.; Mizutani, R.; Satow, Y. (2005) Protein splicing: its discovery and structural insight into novel chemical mechanisms. IUBMB Life 57(8): 563-74. PubMed ID: 16118114
26.  (INT) (REV) Anraku, Y.; Satow, Y. (2009) Reflections on protein splicing: structures, functions and mechanisms. Proc Jpn Acad Ser B Phys Biol Sci 85(9): 409-21. PubMed ID: 19907126
27.  (APP) (TAG) (TS) Appleby, J. H.; Zhou, K.; Volkmann, G.; Liu, X. Q. (2009) Novel split-intein for trans-splicing synthetic peptide onto C-terminus of protein. J Biol Chem 284(10): 6094-6199. PubMed ID: 19136555
28.  (APP) (CPS) (TS) Aranko, A. S.; Zuger, S.; Buchinger, E.; Iwai, H. (2009) In vivo and in vitro protein ligation by naturally occurring and engineered split DnaE inteins. PLoS ONE 4(4): e5185. PubMed ID: 19365564
29.  (INT) Argueso, J. L.; Carazzolle, M. F.; Mieczkowski, P. A.; Duarte, F. M.; Netto, O. V.; Missawa, S. K.; Galzerani, F.; Costa, G. G.; Vidal, R. O.; Noronha, M. F.; Dominska, M.; Andrietta, M. G.; Andrietta, S. R.; Cunha, A. F.; Gomes, L. H.; Tavares, F. C.; Alcarde, A. R.; Dietrich, F. S.; McCusker, J. H.; Petes, T. D.; Pereira, G. A. (2009) Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol production. Genome Res 19(12): 2258-70. PubMed ID: 19812109
30.  (APP) (EPL) Arnold, U.; Hinderaker, M. P.; Nilsson, B. L.; Huck, B. R.; Gellman, S. H.; Raines, R. T. (2002) Protein prosthesis: a semisynthetic enzyme with a beta-peptide reverse turn. J Am Chem Soc 124(29): 8522-3. PubMed ID: 12121081
31.  (APP) (EPL) Arnold, U.; Hinderaker, M. P.; Raines, R. T. (2002B) Semisynthesis of ribonuclease A using intein-mediated protein ligation. ScientificWorldJournal 2: 1823-7. PubMed ID: 12806173
32.  (APP) (EPL) (REV) Arnold, U. (2009) Incorporation of non-natural modules into proteins: structural features beyond the genetic code. Biotechnol Lett 31(8): 1129-39. PubMed ID: 19404746
33.  (INT) ASBMB (2010) Oh Little Intein of Bethlehem: Splicing of the Mycobacteriophage Bethlehem DnaB Intein. Identification of a New Mechanistic Class of Inteins That Contain an Obligate Block F Nucleophile. J Biol Chem 285(4): p. e99910.
34.  (REL) Aspock, G.; Kagoshima, H.; Niklaus, G.; Burglin, T. R. (1999) Caenorhabditis elegans has scores of hedgehog-related genes: sequence and expression analysis. Genome Res 9(10): 909-23. PubMed ID: 10523520
35.  (APP) (CYCL) (EPL) (REL) Austin, J.; Wang, W.; Puttamadappa, S.; Shekhtman, A.; Camarero, J. A. (2009) Biosynthesis and biological screening of a genetically encoded library based on the cyclotide MCoTI-I. Chembiochem 10(16): 2663-70. PubMed ID: 19780078
36.  (APP) (EPL) Ayers, B.; Blaschke, U. K.; Camarero, J. A.; Cotton, G. J.; Holford, M.; Muir, T. W. (1999) Introduction of unnatural amino acids into proteins using expressed protein ligation. Biopolymers 51(5): 343-54. PubMed ID: 10685045
37.  (INT) Bae, H.; Kim, K. P.; Lee, J. I.; Song, J. G.; Kil, E. J.; Kim, J. S.; Kwon, S. T. (2009) Characterization of DNA polymerase from the hyperthermophilic archaeon Thermococcus marinus and its application to PCR. Extremophiles 13(4): 657-67. PubMed ID: 19412677
38.  (EN) (INT) Bae, H.; Kim, K. P.; Song, J. M.; Kim, J. H.; Yang, J. S.; Kwon, S. T. (2009B) Characterization of intein homing endonuclease encoded in the DNA polymerase gene of Thermococcus marinus. FEMS Microbiol Lett 297(2): 180-8. PubMed ID: 19634205
39.  (REL) (REV) Bailey, E. C.; Scott, M. P.; Johnson, R. L. (2000) Hedgehog signaling in animal development and human disease. Ernst Schering Res Found Workshop. (29): 211-35.. PubMed ID: 10943312
40.  (EN) Bakhrat, A.; Jurica, M. S.; Stoddard, B. L.; Raveh, D. (2004) Homology modeling and mutational analysis of HO endonuclease of yeast. Genetics 166(2): 721-8. PubMed ID: 15020462
41.  (EN) (REL) Bakhrat, A.; Baranes, K.; Krichevsky, O.; Rom, I.; Schlenstedt, G.; Pietrokovski, S.; Raveh, D. (2006) Nuclear import of HO endonuclease utilizes two nuclear localization signals and four importins of the ribosomal import system. J Biol Chem 281(18): 12218-26. PubMed ID: 16507575
42.  (INT) Baliga, N. S.; Bonneau, R.; Facciotti, M. T.; Pan, M.; Glusman, G.; Deutsch, E. W.; Shannon, P.; Chiu, Y.; Weng, R. S.; Gan, R. R.; Hung, P.; Date, S. V.; Marcotte, E.; Hood, L.; Ng, W. V. (2004) Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea. Genome Res 14(11): 2221-34. PubMed ID: 15520287
43.  (APP) (PUR) Banki, M. R.; Gerngross, T. U.; Wood, D. W. (2005) Novel and economical purification of recombinant proteins: intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association. Protein Sci 14(6): 1387-95. PubMed ID: 15883185
44.  (APP) (PUR) (REL) Banki, M. R.; Feng, L.; Wood, D. W. (2005B) Simple bioseparations using self-cleaving elastin-like polypeptide tags. Nat Methods 2(9): 659-61. PubMed ID: 16074986
45.  (APP) (PUR) (REV) Banki, M. R.; Wood, D. W. (2005C) Inteins and affinity resin substitutes for protein purification and scale up. Microb Cell Fact 4: 32. PubMed ID: 16283936
46.  (INT) Bao, X.; Carris, L. M.; Huang, G.; Luo, J.; Liu, Y.; Castlebury, L. A. (2010) Tilletia puccinelliae, a new species of reticulate-spored bunt fungus infecting Puccinellia distans. Mycologia 102(3): 613-23. PubMed ID: 20524594
47.  (APP) (EPL) (TAG) Bark, S. J.; Schmid, S.; Hahn, K. M. (2000) A highly efficient method for site-specific modification of unprotected peptides after chemical synthesis. Journal of the American Chemical Society 122(15): 3567-3573.
48.  (APP) (PUR) Barnard, G. C.; McCool, J. D.; Wood, D. W.; Gerngross, T. U. (2005) Integrated recombinant protein expression and purification platform based on Ralstonia eutropha. Appl Environ Microbiol 71(10): 5735-42. PubMed ID: 16204482
49.  (APP) (EPL) Barry, A. N.; Blackburn, N. J. (2008) A selenocysteine variant of the human copper chaperone for superoxide dismutase. A Se-XAS probe of cluster composition at the domain 3-domain 3 dimer interface. Biochemistry 47(17): 4916-28. PubMed ID: 18393442
50.  (APP) (EPL) Barry, A. N.; Clark, K. M.; Otoikhian, A.; van der Donk, W. A.; Blackburn, N. J. (2008B) Selenocysteine Positional Variants Reveal Contributions to Copper Binding from Cysteine Residues in Domains 2 and 3 of Human Copper Chaperone for Superoxide Dismutase. Biochemistry 47(49): 13074-13083. PubMed ID: 19007184
51.  (APP) (EPL) (Structure) Baskaran, S.; Roach, P. J.; DePaoli-Roach, A. A.; Hurley, T. D. (2010) Structural basis for glucose-6-phosphate activation of glycogen synthase. Proc Natl Acad Sci U S A 107(41): 17563-8. PubMed ID: 20876143
52.  (APP) (EPL) (PUR) Bastings, M. M.; van Baal, I.; Meijer, E. W.; Merkx, M. (2008) One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester. BMC Biotechnol 8(1): 76. PubMed ID: 18828922
53.  (APP) (PUR) Baumann, R. G.; Black, L. W. (2003) Isolation and characterization of T4 bacteriophage gp17 terminase, a large subunit multimer with enhanced ATPase activity. J Biol Chem 278(7): 4618-27. PubMed ID: 12466275
54.  (APP) (PUR) Bayrhuber, M.; Graf, R.; Ferber, M.; Zweckstetter, M.; Imperial, J.; Garrett, J. E.; Olivera, B. M.; Terlau, H.; Becker, S. (2006) Production of recombinant Conkunitzin-S1 in Escherichia coli. Protein Expr Purif 47(2): 640-4. PubMed ID: 16542850
55.  (APP) (REL) Bazirgan, O. A.; Garza, R. M.; Hampton, R. Y. (2006) Determinants of RING-E2 fidelity for Hrd1p, a membrane-anchored ubiquitin ligase. J Biol Chem 281(51): 38989-9001. PubMed ID: 17035235
56.  (REL) (REV) Beachy, P. A.; Cooper, M. K.; Young, K. E.; von Kessler, D. P.; Park, W. J.; Hall, T. M.; Leahy, D. J.; Porter, J. A. (1997) Multiple roles of cholesterol in hedgehog protein biogenesis and signaling. Cold Spring Harb Symp Quant Biol 62: 191-204. PubMed ID: 9598352
57.  (INT) (REL) (REV) Becker, Y. (1998) Molecular evolution of viruses--Past and Present, Part 2--An introduction. Virus Genes 16(1): 7-11. PubMed ID: 9562887
58.  (APP) (EPL) Becker, C. F.; Lausecker, K.; Balog, M.; Kalai, T.; Hideg, K.; Steinhoff, H. J.; Engelhard, M. (2005) Incorporation of spin-labelled amino acids into proteins. Magn Reson Chem 43: S34-9. PubMed ID: 16235213
59.  (APP) (EPL) (TAG) Becker, C. F.; Seidel, R.; Jahnz, M.; Bacia, K.; Niederhausen, T.; Alexandrov, K.; Schwille, P.; Goody, R. S.; Engelhard, M. (2006) C-terminal fluorescence labeling of proteins for interaction studies on the single-molecule level. Chembiochem 7(6): 891-5. PubMed ID: 16607669
60.  (APP) (EPL) Becker, C. F. W.; Liu, X.; Olschewski, D.; Castelli, R.; Seidel, R.; Seeberger, P. H. (2008) Semisynthesis of a glycosylphosphatidylinositol-anchored prion protein. Angewandte Chemie - International Edition 47(43): 8215-8219.
61.  (REL) (REV) Begley, T. P.; Xi, J.; Kinsland, C.; Taylor, S.; McLafferty, F. (1999) The enzymology of sulfur activation during thiamin and biotin biosynthesis. Curr Opin Chem Biol 3(5): 623-9. PubMed ID: 10508664
62.  (APP) (REV) Beguin, P. (1999) Hybrid enzymes. Curr Opin Biotechnol 10(4): 336-40. PubMed ID: 10449312
63.  (INT) Belanger, A. S.; Brouard, J. S.; Charlebois, P.; Otis, C.; Lemieux, C.; Turmel, M. (2006) Distinctive architecture of the chloroplast genome in the chlorophycean green alga Stigeoclonium helveticum. Mol Genet Genomics 276(5): 464-77. PubMed ID: 16944205
64.  (REL) (REV) Belfort, M.; Perlman, P. S. (1995) Mechanisms of intron mobility. J Biol Chem 270(51): 30237-40. PubMed ID: 8530436
65.  (INT) (REV) Belfort, M.; Reaban, M. E.; Coetzee, T.; Dalgaard, J. Z. (1995B) Prokaryotic introns and inteins: a panoply of form and function. J Bacteriol 177(14): 3897-903. PubMed ID: 7608058
66.  (EN) (REV) Belfort, M.; Roberts, R. J. (1997) Homing endonucleases: keeping the house in order. Nucleic Acids Res 25(17): 3379-88. PubMed ID: 9254693
67.  (EN) (REL) (REV) Belfort, M. (2003) Two for the price of one: a bifunctional intron-encoded DNA endonuclease-RNA maturase. Genes Dev 17(23): 2860-3. PubMed ID: 14665667
68.  (APP) (EPL) (TAG) Bellmann-Sickert, K.; Baumann, L.; Beck-Sickinger, A. G. (2010) Selective labelling of stromal cell-derived factor 1alpha with carboxyfluorescein to study receptor internalisation. J Pept Sci 16(10): 568-74. PubMed ID: 20862724
69.  (APP) (REV) Benkovic, S. J.; Walsh, C. T. (1997) Research highlights at the chemistry/biology interface. Curr Opin Chem Biol 1(3): 289-91. PubMed ID: 9667881
70.  (APP) (PUR) Berndt, C.; Lillig, C. H.; Wollenberg, M.; Bill, E.; Mansilla, M. C.; de Mendoza, D.; Seidler, A.; Schwenn, J. D. (2004) Characterization and reconstitution of a 4Fe-4S adenylyl sulfate/phosphoadenylyl sulfate reductase from Bacillus subtilis. J Biol Chem 279(9): 7850-5. PubMed ID: 14627706
71.  (APP) (EPL) (REV) Berrade, L.; Camarero, J. A. (2009) Expressed protein ligation: a resourceful tool to study protein structure and function. Cell Mol Life Sci 66(24): 3909-22. PubMed ID: 19685006
72.  (APP) (CPS) (TS) Berrade, L.; Kwon, Y.; Camarero, J. A. (2010) Photomodulation of protein trans-splicing through backbone photocaging of the DnaE split intein. Chembiochem 11(10): 1368-72. PubMed ID: 20512791
73.  (APP) (EPL) Berry, S. M.; Gieselman, M. D.; Nilges, M. J.; van Der Donk, W. A.; Lu, Y. (2002) An engineered azurin variant containing a selenocysteine copper ligand. J Am Chem Soc 124(10): 2084-5. PubMed ID: 11878940
74.  (APP) (EPL) Berry, S. M.; Ralle, M.; Low, D. W.; Blackburn, N. J.; Lu, Y. (2003) Probing the role of axial methionine in the blue copper center of azurin with unnatural amino acids. J Am Chem Soc 125(29): 8760-8. PubMed ID: 12862470
75.  (REL) Bezerra, W. M.; Carvalho, C. P.; Moreira Rde, A.; Grangeiro, T. B. (2006) Establishment of a heterologous system for the expression of Canavalia brasiliensis lectin: a model for the study of protein splicing. Genet Mol Res. 5(1): 216-23. PubMed ID: 16755512
76.  (APP) (NEB) (PUR) (REL) Bitinaite, J.; Rubino, M.; Varma, K. H.; Schildkraut, I.; Vaisvila, R.; Vaiskunaite, R. (2007) USER friendly DNA engineering and cloning method by uracil excision. Nucleic Acids Res 35(6): 1992-2002. PubMed ID: 17341463
77.  (APP) (PUR) Bjorklund, M.; Valtanen, H.; Savilahti, H.; Koivunen, E. (2003) Use of intein-directed peptide biosynthesis to improve serum stability and bioactivity of a gelatinase inhibitory peptide. Comb Chem High Throughput Screen 6(1): 29-35. PubMed ID: 12570750
78.  (INT) Bjornsdottir, S. H.; Blondal, T.; Hreggvidsson, G. O.; Eggertsson, G.; Petursdottir, S.; Hjorleifsdottir, S.; Thorbjarnardottir, S. H.; Kristjansson, J. K. (2006) Rhodothermus marinus: physiology and molecular biology. Extremophiles 10(1): 1-16. PubMed ID: 16075163
79.  (INT) Blackwood, K. S.; He, C.; Gunton, J.; Turenne, C. Y.; Wolfe, J.; Kabani, A. M. (2000) Evaluation of recA sequences for identification of Mycobacterium species. J Clin Microbiol 38(8): 2846-52. PubMed ID: 10921937
80.  (APP) (REV) Blank, K.; Lindner, P.; Diefenbach, B.; Pluckthun, A. (2002) Self-immobilizing recombinant antibody fragments for immunoaffinity chromatography: generic, parallel, and scalable protein purification. Protein Expr Purif 24(2): 313-22. PubMed ID: 11858727
81.  (APP) (EPL) Blaschke, U. K.; Silberstein, J.; Muir, T. W. (2000) Protein engineering by expressed protein ligation. Methods Enzymol 328: 478-96. PubMed ID: 11075362
82.  (APP) (EPL) (NMR) (REV) (Structure) Blaschke, U. K.; Cotton, G.J.; Muir, T. W. (2000B) Synthesis of multi-domain proteins using expressed protein ligation: strategies for segmental labeling of internal regions.. Tetrahedron 56: 9461-9470.
83.  (APP) (EPL) (PUR) Bockhorn, J. J.; Lazar, K. L.; Gasser, A. J.; Luther, L. M.; Qahwash, I. M.; Chopra, N.; Meredith, S. C. (2010) Novel semisynthetic method for generating full length beta-amyloid peptides. Biopolymers 94(4): 511-20. PubMed ID: 20593467
84.  (EN) (INT) Bokor, A. A.; van Kan, J. A.; Poulter, R. T. (2010) Sexual mating of Botrytis cinerea illustrates PRP8 intein HEG activity. Fungal Genet Biol 47(4): 392-8. PubMed ID: 20093192
85.  (INT) Bolhuis, H.; Palm, P.; Wende, A.; Falb, M.; Rampp, M.; Rodriguez-Valera, F.; Pfeiffer, F.; Oesterhelt, D. (2006) The genome of the square archaeon Haloquadratum walsbyi : life at the limits of water activity. BMC Genomics 7: 169. PubMed ID: 16820047
86.  (REL) (Structure) Bompard-Gilles, C.; Villeret, V.; Fanuel, L.; Joris, B.; Frere, J. M.; Van Beeumen, J. (1999) Crystallization and preliminary X-ray analysis of a new L-aminopeptidase-D-amidase/D-esterase activated by a Gly-Ser peptide bond hydrolysis. Acta Crystallogr D Biol Crystallogr 55 ( Pt 3): 699-701. PubMed ID: 10089474
87.  (APP) (PUR) (Structure) Bompard-Gilles, C.; Villeret, V.; Davies, G. J.; Fanuel, L.; Joris, B.; Frere, J. M.; Van Beeumen, J. (2000) A new variant of the Ntn hydrolase fold revealed by the crystal structure of L-aminopeptidase D-ala-esterase/amidase from Ochrobactrum anthropi. Structure 8(2): 153-62. PubMed ID: 10673442
88.  (APP) (PUR) Bonsager, B. C.; Praetorius-Ibba, M.; Nielsen, P. K.; Svensson, B. (2003) Purification and characterization of the beta-trefoil fold protein barley alpha-amylase/subtilisin inhibitor overexpressed in Escherichia coli. Protein Expr Purif 30(2): 185-93. PubMed ID: 12880767
89.  (APP) (PUR) Bonsager, B. C.; Nielsen, P. K.; Abou Hachem, M.; Fukuda, K.; Praetorius-Ibba, M.; Svensson, B. (2005) Mutational analysis of target enzyme recognition of the beta-trefoil fold barley alpha-amylase/subtilisin inhibitor. J Biol Chem 280(15): 14855-64. PubMed ID: 15657043
90.  (APP) (EPL) (REV) Borgia, J. A.; Fields, G. B. (2000) Chemical synthesis of proteins. Trends Biotechnol 18(6): 243-51. PubMed ID: 10802559
91.  (REL) (REV) Borissenko, L.; Groll, M. (2007) Diversity of proteasomal missions: fine tuning of the immune response. Biol Chem 388(9): 947-55. PubMed ID: 17696779
92.  (APP) (NMR) (REV) (Structure) Borman, S. (1999) Scaling Up Protein NMR: Segmental labeling technique improves access to 3-D structures of large proteins. Chem & Eng News February 15: 65-67.
93.  (APP) (CPS) (REV) Borman, S. (2002) Controlled Protein Splicing. Chem & Eng News 13: 289.
94.  (APP) (EPL) Borodovsky, A.; Ovaa, H.; Kolli, N.; Gan-Erdene, T.; Wilkinson, K. D.; Ploegh, H. L.; Kessler, B. M. (2002) Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family. Chem Biol 9(10): 1149-59. PubMed ID: 12401499
95.  (APP) (NMR) (REV) (Structure) Bott, R.; Boelens, R. (1999) The role of high-resolution structural studies in the development of commercial enzymes. Curr Opin Biotechnol 10(4): 391-7. PubMed ID: 10449323
96.  (INT) (NEB) Brace, L. E.; Southworth, M. W.; Tori, K.; Cushing, M. L.; Perler, F. (2010) The Deinococcus radiodurans Snf2 intein caught in the act: detection of the Class 3 intein signature Block F branched intermediate. Protein Sci 19(8): 1525-33. PubMed ID: 20521254
97.  (APP) (PUR) Bradley, L. H.; Kleiner, R. E.; Wang, A. F.; Hecht, M. H.; Wood, D. W. (2005) An intein-based genetic selection allows the construction of a high-quality library of binary patterned de novo protein sequences. Protein Eng Des Sel 18(4): 201-7. PubMed ID: 15849217
98.  (APP) (PUR) Brandt, J. J.; Chatwood, L. L.; Crowder, M. W. (2000) Analysis of three overexpression systems for VanX, the Zinc(II) dipeptidase required for high-level vancomycin resistance in bacteria. Protein Expr Purif 20(2): 300-7. PubMed ID: 11049753
99.  (EN) (INT) Bremer, M. C.; Gimble, F. S.; Thorner, J.; Smith, C. L. (1992) VDE endonuclease cleaves Saccharomyces cerevisiae genomic DNA at a single site: physical mapping of the VMA1 gene. Nucleic Acids Res 20(20): 5484. PubMed ID: 1437572
100.  (REL) Brennan, T. V.; Clarke, S. (1993) Spontaneous degradation of polypeptides at aspartyl and asparaginyl residues: effects of the solvent dielectric. Protein Sci 2(3): 331-8. PubMed ID: 8453372
101.  (REL) Brennan, T. V.; Clarke, S. (1993B) Mechanism of cleavage at Asn 148 during the maturation of jack bean concanavalin A. Biochem Biophys Res Commun 193(3): 1031-7. PubMed ID: 8323528
102.  (REL) Brennan, T. V.; Clarke, S. (1995) Effect of adjacent histidine and cysteine residues on the spontaneous degradation of asparaginyl- and aspartyl-containing peptides. Int J Pept Protein Res 45(6): 547-53. PubMed ID: 7558585
103.  (APP) (EN) Brenneman, M.; Gimble, F. S.; Wilson, J. H. (1996) Stimulation of intrachromosomal homologous recombination in human cells by electroporation with site-specific endonucleases. Proc Natl Acad Sci U S A 93(8): 3608-12. PubMed ID: 8622983
104.  (APP) (CPS) (TS) Brenzel, S.; Mootz, H. D. (2005) Design of an intein that can be inhibited with a small molecule ligand. J Am Chem Soc 127(12): 4176-7. PubMed ID: 15783192
105.  (APP) (CPS) (INT) (TS) Brenzel, S.; Kurpiers, T.; Mootz, H. D. (2006) Engineering Artificially Split Inteins for Applications in Protein Chemistry: Biochemical Characterization of the Split Ssp DnaB Intein and Comparison to the Split Sce VMA Intein. Biochemistry 45(6): 1571-8. PubMed ID: 16460004
106.  (APP) (TS) Brenzel, S.; Cebi, M.; Reiss, P.; Koert, U.; Mootz, H. D. (2009) Expanding the scope of protein trans-splicing to fragment ligation of an integral membrane protein: towards modulation of porin-based ion channels by chemical modification. Chembiochem 10(6): 983-6. PubMed ID: 19322843
107.  (INT) Brosch, R.; Gordon, S. V.; Garnier, T.; Eiglmeier, K.; Frigui, W.; Valenti, P.; Dos Santos, S.; Duthoy, S.; Lacroix, C.; Garcia-Pelayo, C.; Inwald, J. K.; Golby, P.; Garcia, J. N.; Hewinson, R. G.; Behr, M. A.; Quail, M. A.; Churcher, C.; Barrell, B. G.; Parkhill, J.; Cole, S. T. (2007) Genome plasticity of BCG and impact on vaccine efficacy. Proc Natl Acad Sci U S A 104(13): 5596-601. PubMed ID: 17372194
108.  (REL) (REV) Brugger, K.; Redder, P.; She, Q.; Confalonieri, F.; Zivanovic, Y.; Garrett, R. A. (2002) Mobile elements in archaeal genomes. FEMS Microbiol Lett. 206(2): 131-41.. PubMed ID: 11814653
109.  (INT) Brugger, K.; Chen, L.; Stark, M.; Zibat, A.; Redder, P.; Ruepp, A.; Awayez, M.; She, Q.; Garrett, R. A.; Klenk, H. P. (2007) The genome of Hyperthermus butylicus: a sulfur-reducing, peptide fermenting, neutrophilic Crenarchaeote growing up to 108 degrees C. Archaea 2(2): 127-35. PubMed ID: 17350933
110.  (APP) (EPL) Brunsveld, L.; Watzke, A.; Durek, T.; Alexandrov, K.; Goody, R. S.; Waldmann, H. (2005) Synthesis of functionalized rab GTPases by a combination of solution- or solid-phase lipopeptide synthesis with expressed protein ligation. Chemistry 11(9): 2756-72. PubMed ID: 15729676
111.  (APP) (EPL) (REL) Brunsveld, L.; Kuhlmann, J.; Alexandrov, K.; Wittinghofer, A.; Goody, R. S.; Waldmann, H. (2006) Lipidated ras and rab peptides and proteins--synthesis, structure, and function. Angew Chem Int Ed Engl 45(40): 6622-46. PubMed ID: 17031879
112.  (APP) (NMR) (TS) Buchinger, E.; Aachmann, F. L.; Aranko, A. S.; Valla, S.; Skjak-Braek, G.; Iwai, H.; Wimmer, R. (2010) Use of protein trans-splicing to produce active and segmentally (2)H, (15)N labeled mannuronan C5-epimerase AlgE4. Protein Sci 19(8): 1534-43. PubMed ID: 20552686
113.  (APP) (EPL) (REV) Budisa, N. (2004) Adding new tools to the arsenal of expressed protein ligation. Chembiochem 5(9): 1176-9. PubMed ID: 15368567
114.  (INT) Buell, C. R.; Joardar, V.; Lindeberg, M.; Selengut, J.; Paulsen, I. T.; Gwinn, M. L.; Dodson, R. J.; Deboy, R. T.; Durkin, A. S.; Kolonay, J. F.; Madupu, R.; Daugherty, S.; Brinkac, L.; Beanan, M. J.; Haft, D. H.; Nelson, W. C.; Davidsen, T.; Zafar, N.; Zhou, L.; Liu, J.; Yuan, Q.; Khouri, H.; Fedorova, N.; Tran, B.; Russell, D.; Berry, K.; Utterback, T.; Van Aken, S. E.; Feldblyum, T. V.; D'Ascenzo, M.; Deng, W. L.; Ramos, A. R.; Alfano, J. R.; Cartinhour, S.; Chatterjee, A. K.; Delaney, T. P.; Lazarowitz, S. G.; Martin, G. B.; Schneider, D. J.; Tang, X.; Bender, C. L.; White, O.; Fraser, C. M.; Collmer, A. (2003) The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000. Proc Natl Acad Sci U S A 100(18): 10181-6. PubMed ID: 12928499
115.  (INT) Bult, C. J.; White, O.; Olsen, G. J.; Zhou, L.; Fleischmann, R. D.; Sutton, G. G.; Blake, J. A.; FitzGerald, L. M.; Clayton, R. A.; Gocayne, J. D.; Kerlavage, A. R.; Dougherty, B. A.; Tomb, J. F.; Adams, M. D.; Reich, C. I.; Overbeek, R.; Kirkness, E. F.; Weinstock, K. G.; Merrick, J. M.; Glodek, A.; Scott, J. L.; Geoghagen, N. S.; Venter, J. C. (1996) Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science 273(5278): 1058-73. PubMed ID: 8688087
116.  (APP) (EPL) (TAG) Burbulis, I.; Yamaguchi, K.; Gordon, A.; Carlson, R.; Brent, R. (2005) Using protein-DNA chimeras to detect and count small numbers of molecules. Nat Methods 2(1): 31-7. PubMed ID: 15782158
117.  (REL) (REV) Burglin, T. R. (1996) Warthog and groundhog, novel families related to hedgehog. Curr Biol 6(9): 1047-50. PubMed ID: 8805384
118.  (REL) (REV) Burglin, T. R. (2008) Evolution of hedgehog and hedgehog-related genes, their origin from Hog proteins in ancestral eukaryotes and discovery of a novel Hint motif. BMC Genomics 9: 127. PubMed ID: 18334026
119.  (REL) Burglin, T. R. (2008B) The Hedgehog protein family. Genome Biol 9(11): 241. PubMed ID: 19040769
120.  (EN) (REV) Burt, A.; Koufopanou, V. (2004) Homing endonuclease genes: the rise and fall and rise again of a selfish element. Curr Opin Genet Dev 14(6): 609-15. PubMed ID: 15531154
121.  (APP) (NMR) (TS) Busche, A. E.; Aranko, A. S.; Talebzadeh-Farooji, M.; Bernhard, F.; Dotsch, V.; Iwai, H. (2009) Segmental Isotopic Labeling of a Central Domain in a Multidomain Protein by Protein Trans-Splicing Using Only One Robust DnaE Intein. Angew Chem Int Ed Engl 48(33): 6128-6131. PubMed ID: 19591176
122.  (APP) (EPL) (TAG) Buskas, T.; Ingale, S.; Boons, G. J. (2006) Glycopeptides as versatile tools for glycobiology. Glycobiology 16(8): 113R-136R. PubMed ID: 16675547
123.  (APP) (CPS) Buskirk, A. R.; Ong, Y. C.; Gartner, Z. J.; Liu, D. R. (2004) Directed evolution of ligand dependence: small-molecule-activated protein splicing. Proc Natl Acad Sci U S A 101(29): 10505-10. PubMed ID: 15247421
124.  (INT) Butler, M. I.; Goodwin, T. J.; Poulter, R. T. (2001) A nuclear-encoded intein in the fungal pathogen Cryptococcus neoformans. Yeast 18(15): 1365-70. PubMed ID: 11746598
125.  (EN) (INT) Butler, M. I.; Poulter, R. T. (2005) The PRP8 inteins in Cryptococcus are a source of phylogenetic and epidemiological information. Fungal Genet Biol 42(5): 452-63. PubMed ID: 15809009
126.  (INT) Butler, M. I.; Goodwin, T. J.; Poulter, R. T. (2005B) Two new fungal inteins. Yeast 22(6): 493-501. PubMed ID: 15849795
127.  (EN) (INT) Butler, M. I.; Gray, J.; Goodwin, T. J.; Poulter, R. T. (2006) The distribution and evolutionary history of the PRP8 intein. BMC Evol Biol. 6(1): 42. PubMed ID: 16737526
128.  (APP) (REL) Camarero, J. A.; Muir, T. W. (1997) Chemoselective backbone cyclization of unprotected peptides. Chem Commun 1997: 1369-1370.
129.  (APP) (CYCL) Camarero, J. A.; Pavel, J.; Muir, T. W. (1998) Chemical Synthesis of a Circular Protein Domain: Evidence for Folding-Assisted Cyclization. Angew. Chem. Int. Ed. 37: 347-349.
130.  (APP) (REL) (REV) Camarero, J.A. ; Muir, T.W. (1999) Native Chemical Ligation of Polypeptides. Current Protocols in Protein Science 18.4.1-18.4.21.
131.  (APP) (CYCL) Camarero, J. A.; Muir, T. W. (1999B) Biosynthesis of a Head-to Tail Cyclized Protein with Improved Biological Activity. J. Amer. Chem. Soc. 121: 5597-5598.
132.  (APP) (REL) Camarero, J. A.; Fushman, D.; Sato, S.; Giriat, I.; Cowburn, D.; Raleigh, D. P.; Muir, T. W. (2001) Rescuing a destabilized protein fold through backbone cyclization. J Mol Biol 308(5): 1045-62. PubMed ID: 11352590
133.  (APP) (CYCL) Camarero, J. A.; Fushman, D.; Cowburn, D.; Muir, T. W. (2001B) Peptide chemical ligation inside living cells: in vivo generation of a circular protein domain. Bioorg Med Chem 9(9): 2479-84. PubMed ID: 11553489
134.  (APP) (EPL) (NMR) (PUR) (Structure) Camarero, J. A.; Shekhtman, A.; Campbell, E. A.; Chlenov, M.; Gruber, T. M.; Bryant, D. A.; Darst, S. A.; Cowburn, D.; Muir, T. W. (2002) Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR. Proc Natl Acad Sci U S A 99(13): 8536-41. PubMed ID: 12084914
135.  (EPL) (REL) Camarero, J. A.; Hackel, B. J.; de Yoreo, J. J.; Mitchell, A. R. (2004) Fmoc-based synthesis of peptide alpha-thioesters using an aryl hydrazine support. J Org Chem 69(12): 4145-51. PubMed ID: 15176841
136.  (APP) (EPL) (TAG) Camarero, J. A.; Kwon, Y.; Coleman, M. A. (2004B) Chemoselective attachment of biologically active proteins to surfaces by expressed protein ligation and its application for "protein chip" fabrication. J Am Chem Soc 126(45): 14730-1. PubMed ID: 15535692
137.  (APP) (EPL) (REL) (REV) Camarero, J. A.; Mitchell, A. R. (2005) Synthesis of proteins by native chemical ligation using Fmoc-based chemistry. Protein Pept Lett 12(8): 723-8. PubMed ID: 16305540
138.  (APP) (CYCL) (PUR) Camarero, J. A.; Kimura, R. H.; Woo, Y. H.; Shekhtman, A.; Cantor, J. (2007) Biosynthesis of a fully functional cyclotide inside living bacterial cells. Chembiochem 8(12): 1363-1366.
139.  (APP) (EPL) (REV) (TAG) Camarero, J. A. (2008) Recent developments in the site-specific immobilization of proteins onto solid supports. Biopolymers 90(3): 450-8. PubMed ID: 17618518
140.  (APP) (REV) (TAG) (TS) Camarero, J. A.; Kwon, Y. (2008B) Traceless and Site-specific Attachment of Proteins onto Solid Supports. International Journal of Peptide Research and Therapeutics 14: 351-357.
141.  (APP) (EPL) (REV) (TS) Camarero, Julio A. (2009) Optimizing the future for biotechnology therapies, the key role of protein engineering. Advanced Drug Delivery Reviews 61(11): 897-898.
142.  (INT) Cambon-Bonavita, M. A.; Schmitt, P.; Zieger, M.; Flaman, J. M.; Lesongeur, F.; Raguenes, G.; Bindel, D.; Frisch, N.; Lakkis, Z.; Dupret, D.; Barbier, G.; Querellou, J. (2000) Cloning, expression, and characterization of DNA polymerase I from the hyperthermophilic archaea Thermococcus fumicolans. Extremophiles 4(4): 215-25. PubMed ID: 10972190
143.  (APP) (CPS) (NEB) Cann, I. K.; Amaya, K. R.; Southworth, M. W.; Perler, F. B. (2004) Bacteriophage-based genetic system for selection of nonsplicing inteins. Appl Environ Microbiol 70(5): 3158-62. PubMed ID: 15128583
144.  (APP) (NEB) (PUR) Cantor, E. J.; Chong, S. (2001) Intein-mediated rapid purification of Cre recombinase. Protein Expr Purif 22(1): 135-40. PubMed ID: 11388811
145.  (REL) Capasso, S.; Mazzarella, L.; Sorrentino, G.; Balboni, G.; Kirby, A. J. (1996) Kinetics and mechanism of the cleavage of the peptide bond next to asparagine. Peptides 17(6): 1075-7. PubMed ID: 8899829
146.  (INT) (REL) Carter, A. T.; Narbad, A.; Pearson, B. M.; Beck, K. F.; Logghe, M.; Contreras, R.; Schweizer, M. (1994) Phosphoribosylpyrophosphate synthetase (PRS): a new gene family in Saccharomyces cerevisiae. Yeast 10(8): 1031-44. PubMed ID: 7992503
147.  (INT) (REL) Carter, A. T.; Beiche, F.; Hove-Jensen, B.; Narbad, A.; Barker, P. J.; Schweizer, L. M.; Schweizer, M. (1997) PRS1 is a key member of the gene family encoding phosphoribosylpyrophosphate synthetase in Saccharomyces cerevisiae. Mol Gen Genet 254(2): 148-56. PubMed ID: 9108276
148.  (APP) (EPL) (REL) (REV) Casi, Giulio; Hilvert, Donald (2003) Convergent protein synthesis. Current Opinion in Structural Biology 13(5): 589-594.
149.  (INT) Caspi, J.; Amitai, G.; Belenkiy, O.; Pietrokovski, S. (2003) Distribution of split DnaE inteins in cyanobacteria. Mol Microbiol 50(5): 1569-77. PubMed ID: 14651639
150.  (APP) (PUR) Cattoli, Francesca; Sarti, Giulio C. (2002) Characterization of amylose affinity membranes through adsorption of pure recombinant MBP-intein-CBD: equilibrium and kinetics data. Desalination 149(1-3): 465-470.
151.  (APP) (PUR) Cattoli, Francesca; Boi, Cristiana; Sorci, Mirco; Sarti, Giulio C. (2006) Adsorption of pure recombinant MBP-fusion proteins on amylose affinity membranes. Journal of Membrane Science 273(1-2): 2-11.
152.  (APP) (EPL) (TAG) Ceccarini, G.; Flavell, R. R.; Butelman, E. R.; Synan, M.; Willnow, T. E.; Bar-Dagan, M.; Goldsmith, S. J.; Kreek, M. J.; Kothari, P.; Vallabhajosula, S.; Muir, T. W.; Friedman, J. M. (2009) PET imaging of leptin biodistribution and metabolism in rodents and primates. Cell Metab 10(2): 148-59. PubMed ID: 19656493
153.  (APP) (PUR) Celis, R. T. (1999) Repression and activation of arginine transport genes in Escherichia coli K 12 by the ArgP protein. J Mol Biol 294(5): 1087-95. PubMed ID: 10600368
154.  (APP) (EPL) (Structure) Chacko, B. M.; Qin, B. Y.; Tiwari, A.; Shi, G.; Lam, S.; Hayward, L. J.; De Caestecker, M.; Lin, K. (2004) Structural basis of heteromeric smad protein assembly in TGF-beta signaling. Mol Cell 15(5): 813-23. PubMed ID: 15350224
155.  (APP) (EPL) (TAG) Chaisemartin, L.; Chinestra, P.; Favre, G.; Blonski, C.; Faye, J. C. (2010) Synthesis and Application of a N-1' Fluorescent Biotinyl Derivative Inducing the Specific Carboxy-Terminal Dual Labeling of a Novel RhoB-Selective scFv. Bioconjug Chem 20(5): 847-855. PubMed ID: 19348471
156.  (APP) (REV) (TAG) Chamberlain, C.; Hahn, K. M. (2000) Watching proteins in the wild: fluorescence methods to study protein dynamics in living cells. Traffic 1(10): 755-62. PubMed ID: 11208065
157.  (APP) (EPL) (NEB) Chan, S. H.; Zhu, Z.; Dunigan, D. D.; Van Etten, J. L.; Xu, S. Y. (2006) Cloning of Nt.CviQII nicking endonuclease and its cognate methyltransferase: M.CviQII methylates AG sequences. Protein Expr Purif 49(1): 138-50. PubMed ID: 16737828
158.  (APP) (EPL) Chang, M. C.; Yee, C. S.; Nocera, D. G.; Stubbe, J. (2004) Site-specific replacement of a conserved tyrosine in ribonucleotide reductase with an aniline amino acid: a mechanistic probe for a redox-active tyrosine. J Am Chem Soc 126(51): 16702-3. PubMed ID: 15612690
159.  (APP) (TAG) (TS) Charalambous, A.; Andreou, M.; Skourides, P. A. (2009) Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo. J Nanobiotechnology 7: 9. PubMed ID: 20003264
160.  (APP) (EPL) (TAG) Chatterjee, C.; McGinty, R. K.; Pellois, J. P.; Muir, T. W. (2007) Auxiliary-mediated site-specific peptide ubiquitylation. Angew Chem Int Ed Engl 46(16): 2814-8. PubMed ID: 17366504
161.  (APP) (EPL) (TAG) Chatterjee, C.; McGinty, R. K.; Fierz, B.; Muir, T. W. (2010) Disulfide-directed histone ubiquitylation reveals plasticity in hDot1L activation. Nat Chem Biol 6(4): 267-9. PubMed ID: 20208522
162.  (APP) (EPL) Chatterjee, C.; Muir, T. W. (2010B) Chemical approaches for studying histone modifications. J Biol Chem 285(15): 11045-50. PubMed ID: 20147749
163.  (APP) (EPL) (PUR) (REV) Chattopadhaya, S.; Tan, L. P.; Yao, S. Q. (2006) Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays. Nat Protoc 1(5): 2386-98. PubMed ID: 17406482
164.  (APP) (EPL) (PUR) (REV) Chattopadhaya, S.; Srinivasan, R.; Yeo, D. S.; Chen, G. Y.; Yao, S. Q. (2009) Site-specific covalent labeling of proteins inside live cells using small molecule probes. Bioorg Med Chem 17(3): 981-9. PubMed ID: 18261914
165.  (APP) (EPL) (REV) (TAG) Chattopadhaya, S.; Abu Bakar, F. B.; Yao, S. Q. (2009B) Use of intein-mediated protein ligation strategies for the fabrication of functional protein arrays. Methods Enzymol 462: 195-223. PubMed ID: 19632476
166.  (APP) (PUR) Che, N.; Wang, L.; Gao, Y.; An, C. (2009) Soluble expression and one-step purification of a neurotoxin Huwentoxin-I in Escherichia coli. Protein Expr Purif 65(2): 154-9. PubMed ID: 19217942
167.  (INT) (NEB) Chen, L.; Benner, J.; Perler, F. B. (2000) Protein splicing in the absence of an intein penultimate histidine. J Biol Chem 275(27): 20431-5. PubMed ID: 10770923
168.  (APP) (NEB) (TS) Chen, L.; Pradhan, S.; Evans, T. C., Jr. (2001) Herbicide resistance from a divided EPSPS protein: the split Synechocystis DnaE intein as an in vivo affinity domain. Gene 263(1-2): 39-48. PubMed ID: 11223241
169.  (INT) (NEB) (Structure) Chen, X.; Xu, M. Q.; Ding, Y.; Ferrandon, S.; Rao, Z. (2002) Purification and initial crystallization studies of a DnaB intein from Synechocystis sp. PCC 6803. Acta Crystallogr D Biol Crystallogr 58(Pt 7): 1201-3. PubMed ID: 12077443
170.  (INT) (NEB) (Structure) (TS) Chen, X.; Xu, M. Q.; Ding, Y.; Ferrandon, S.; Rao, Z. (2002B) Crystallographic study of a naturally occurring trans-splicing intein from Synechocystis sp. PCC 6803. Acta Crystallogr D Biol Crystallogr 58(Pt 7): 1204-6. PubMed ID: 12077444
171.  (APP) (NEB) (PUR) Chen, L. Y.; Yi, X. Y.; Zeng, X. F.; Zhang, S. K.; McReynolds, L. (2004) [Mucosal immunization of recombinant Schistosoma japonicum ferritin]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 22(3): 129-32. PubMed ID: 15597704
172.  (APP) (PUR) Chen, H. W.; North, S. H.; Nakai, H. (2004) Properties of the PriA helicase domain and its role in binding PriA to specific DNA structures. J Biol Chem 279(37): 38503-12. PubMed ID: 15252043
173.  (APP) (NEB) (PUR) Chen, L. Y.; Yi, X. Y.; Zeng, X. F.; Cai, C.; McReynolds, L. (2005) A new antigen gene of Schistosoma japonicum: Screening, cloning, expression and immunization in mice. Progress in Biochemistry and Biophysics 32(4): 331-337.
174.  (APP) (PUR) (TS) Chen, L.; Zhu, F.; Li, J.; Lu, H.; Jiang, H.; Sarkar, R.; Arruda, V. R.; Wang, J.; Zhao, J.; Pierce, G. F.; Ding, Q.; Wang, X.; Wang, H.; Pipe, S. W.; Liu, X. Q.; Xiao, X.; Camire, R. M.; Xiao, W. (2007) The enhancing effects of the light chain on heavy chain secretion in split delivery of factor VIII gene. Mol Ther 15(10): 1856-62. PubMed ID: 17653101
175.  (APP) (PUR) Chen, Y. Q.; Zhang, S. Q.; Li, B. C.; Qiu, W.; Jiao, B.; Zhang, J.; Diao, Z. Y. (2008) Expression of a cytotoxic cationic antibacterial peptide in Escherichia coli using two fusion partners. Protein Expr Purif 57(2): 303-11. PubMed ID: 17977015
176.  (APP) (PUR) Chen, Y. Q.; Qiu, W.; Jiao, B.; Zhang, J.; Zhang, S. Q. (2008) Fusion expression of antibacterial and antitumorial peptide CM4N in Escherichia coli and its activity identification. Pharmaceutical Biotechnology 15(3): 161-165.
177.  (INT) Chen, C. L.; Pan, T. Y.; Kan, S. C.; Kuan, Y. C.; Hong, L. Y.; Chiu, K. R.; Sheu, C. S.; Yang, J. S.; Hsu, W. H.; Hu, H. Y. (2008B) Genome sequence of the lytic bacteriophage P1201 from Corynebacterium glutamicum NCHU 87078: evolutionary relationships to phages from Corynebacterineae. Virology 378(2): 226-32. PubMed ID: 18599103
178.  (APP) (EPL) (TAG) Chen, J.; Ai, Y.; Wang, J.; Haracska, L.; Zhuang, Z. (2010) Chemically ubiquitylated PCNA as a probe for eukaryotic translesion DNA synthesis. Nat Chem Biol 6(4): 270-2. PubMed ID: 20208521
179.  (APP) (TS) Chen, L.; Zhang, Y.; Li, G.; Huang, H.; Zhou, N. (2010B) Functional characterization of a naturally occurring trans-splicing intein from Synechococcus elongatus in a mammalian cell system. Anal Biochem 407(2): 180-7. PubMed ID: 20727340
180.  (APP) (CYCL) (TAG) Cheng, L.; Naumann, T. A.; Horswill, A. R.; Hong, S. J.; Venters, B. J.; Tomsho, J. W.; Benkovic, S. J.; Keiler, K. C. (2007) Discovery of antibacterial cyclic peptides that inhibit the ClpXP protease. Protein Sci 16(8): 1535-42. PubMed ID: 17600141
181.  (APP) (CPS) (EPL) (NEB) (REV) (TS) Cheriyan, M.; Perler, F. B. (2009) Protein splicing: A versatile tool for drug discovery. Adv Drug Deliv Rev 61(11): 899-907. PubMed ID: 19442693
182.  (INT) Chervitz, S. A.; Aravind, L.; Sherlock, G.; Ball, C. A.; Koonin, E. V.; Dwight, S. S.; Harris, M. A.; Dolinski, K.; Mohr, S.; Smith, T.; Weng, S.; Cherry, J. M.; Botstein, D. (1998) Comparison of the complete protein sets of worm and yeast: orthology and divergence. Science 282(5396): 2022-8. PubMed ID: 9851918
183.  (EN) Chevalier, B. S.; Stoddard, B. L. (2001) Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility. Nucleic Acids Res 29(18): 3757-74. PubMed ID: 11557808
184.  (EN) Chevalier, B.; Sussman, D.; Otis, C.; Noel, A. J.; Turmel, M.; Lemieux, C.; Stephens, K.; Monnat, R. J., Jr.; Stoddard, B. L. (2004) Metal-dependent DNA cleavage mechanism of the I-CreI LAGLIDADG homing endonuclease. Biochemistry 43(44): 14015-26. PubMed ID: 15518550
185.  (APP) (PUR) Chiang, C. J.; Chen, H. C.; Chao, Y. P.; Tzen, J. T. (2005) Efficient system of artificial oil bodies for functional expression and purification of recombinant nattokinase in Escherichia coli. J Agric Food Chem 53(12): 4799-804. PubMed ID: 15941319
186.  (APP) (PUR) (TAG) Chiang, C. J.; Chen, H. C.; Chao, Y. P.; Tzen, J. T. (2007) One-step purification of insoluble hydantoinase overproduced in Escherichia coli. Protein Expr Purif 52(1): 14-8. PubMed ID: 16920364
187.  (APP) (EPL) (REV) Chiang, K. P.; Muir, T. W. (2008) Systems- and molecular-level elucidation of signaling processes through chemistry. Sci Signal 1(42): pe45. PubMed ID: 18941136
188.  (APP) (EPL) Chiang, K. P.; Jensen, M. S.; McGinty, R. K.; Muir, T. W. (2009) A semisynthetic strategy to generate phosphorylated and acetylated histone H2B. Chembiochem 10(13): 2182-7. PubMed ID: 19623598
189.  (REV) Chin, G.J. (2000) Forming New Ties Directly. Science 289(5488): 2243.
190.  (APP) (NEB) (TS) Chin, H. G.; Kim, G. D.; Marin, I.; Mersha, F.; Evans, T. C., Jr.; Chen, L.; Xu, M. Q.; Pradhan, S. (2003) Protein trans-splicing in transgenic plant chloroplast: reconstruction of herbicide resistance from split genes. Proc Natl Acad Sci U S A 100(8): 4510-5. PubMed ID: 12671070
191.  (APP) (NEB) (PUR) Chin, H. G.; Patnaik, D.; Esteve, P. O.; Jacobsen, S. E.; Pradhan, S. (2006) Catalytic properties and kinetic mechanism of human recombinant Lys-9 histone H3 methyltransferase SUV39H1: participation of the chromodomain in enzymatic catalysis. Biochemistry 45(10): 3272-84. PubMed ID: 16519522
192.  (INT) Chivian, D.; Brodie, E. L.; Alm, E. J.; Culley, D. E.; Dehal, P. S.; Desantis, T. Z.; Gihring, T. M.; Lapidus, A.; Lin, L. H.; Lowry, S. R.; Moser, D. P.; Richardson, P. M.; Southam, G.; Wanger, G.; Pratt, L. M.; Andersen, G. L.; Hazen, T. C.; Brockman, F. J.; Arkin, A. P.; Onstott, T. C. (2008) Environmental genomics reveals a single-species ecosystem deep within Earth. Science 322(5899): 275-8. PubMed ID: 18845759
193.  (INT) (TS) Choi, J. J.; Nam, K. H.; Min, B.; Kim, S. J.; Soll, D.; Kwon, S. T. (2006) Protein trans-splicing and characterization of a split family B-type DNA polymerase from the hyperthermophilic archaeal parasite Nanoarchaeum equitans. J Mol Biol 356(5): 1093-106. PubMed ID: 16412462
194.  (INT) (NEB) Chong, S.; Shao, Y.; Paulus, H.; Benner, J.; Perler, F. B.; Xu, M. Q. (1996) Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system. J Biol Chem 271(36): 22159-68. PubMed ID: 8703028
195.  (APP) (NEB) (PUR) Chong, S.; Mersha, F. B.; Comb, D. G.; Scott, M. E.; Landry, D.; Vence, L. M.; Perler, F. B.; Benner, J.; Kucera, R. B.; Hirvonen, C. A.; Pelletier, J. J.; Paulus, H.; Xu, M. Q. (1997) Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192(2): 271-81. PubMed ID: 9224900
196.  (EN) (INT) (NEB) Chong, S.; Xu, M. Q. (1997B) Protein splicing of the Saccharomyces cerevisiae VMA intein without the endonuclease motifs. J Biol Chem 272(25): 15587-90. PubMed ID: 9188443
197.  (INT) (NEB) Chong, S.; Williams, K. S.; Wotkowicz, C.; Xu, M. Q. (1998) Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein. J Biol Chem 273(17): 10567-77. PubMed ID: 9553117
198.  (NEB) (PUR) Chong, S.; Montello, G. E.; Zhang, A.; Cantor, E. J.; Liao, W.; Xu, M. Q.; Benner, J. (1998B) Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acids Res 26(22): 5109-15. PubMed ID: 9801307
199.  (APP) (NEB) (PUR) (REV) Chong, S.; Perler, F. (2003) Intein-mediated Protein Purifications. In: Prokaryotic Genomics. Methods and Tools in Biosciences and Medicine, ed. M. Blot. (Birkhauser Verlag) pages 173-193.
200.  (APP) (NEB) (PUR) (REV) Chong, S.; Xu, M-Q. (2005) Harnessing Inteins for Protein Purifications and Characterization. In: Homing Endonucleases and Inteins, ed. Belfort, M.; Derbyshire, V.; Stoddard, B.L.; Wood, D.W.. (Springer-Verlag, Heidelberg, Germany) Vol. 16 pages 273-292.
201.  (INT) Chopin, A.; Deveau, H.; Ehrlich, S. D.; Moineau, S.; Chopin, M. C. (2007) KSY1, a lactococcal phage with a T7-like transcription. Virology 365(1): 1-9. PubMed ID: 17467024
202.  (APP) (PUR) Chorostowska-Wynimko, J.; Swiercz, R.; Skrzypczak-Jankun, E.; Wojtowicz, A.; Selman, S. H.; Jankun, J. (2003) A novel form of the plasminogen activator inhibitor created by cysteine mutations extends its half-life: relevance to cancer and angiogenesis. Mol Cancer Ther 2(1): 19-28. PubMed ID: 12533669
203.  (EN) (INT) Christ, F.; Schoettler, S.; Wende, W.; Steuer, S.; Pingoud, A.; Pingoud, V. (1999) The monomeric homing endonuclease PI-SceI has two catalytic centres for cleavage of the two strands of its DNA substrate. Embo J 18(24): 6908-16. PubMed ID: 10601013
204.  (EN) (INT) Christ, F.; Steuer, S.; Thole, H.; Wende, W.; Pingoud, A.; Pingoud, V. (2000) A model for the PI-SceIxDNA complex based on multiple base and phosphate backbone-specific photocross-links. J Mol Biol 300(4): 867-75. PubMed ID: 10891273
205.  (APP) (PUR) Christensen, S. K.; Mikkelsen, M.; Pedersen, K.; Gerdes, K. (2001) RelE, a global inhibitor of translation, is activated during nutritional stress. Proc Natl Acad Sci U S A 98(25): 14328-33. PubMed ID: 11717402
206.  (APP) (PUR) (TAG) (TS) Chu, N. K.; Becker, C. F. (2009) Semisynthesis of membrane-attached prion proteins. Methods Enzymol 462: 177-93. PubMed ID: 19632475
207.  (APP) (TAG) (TS) Chu, N. K.; Olschewski, D.; Seidel, R.; Winklhofer, K. F.; Tatzelt, J.; Engelhard, M.; Becker, C. F. (2010) Protein immobilization on liposomes and lipid-coated nanoparticles by protein trans-splicing. J Pept Sci 16(10): 582-8. PubMed ID: 20862726
208.  (APP) (PUR) Churchill, R. L.; Lee, H.; Hall, J. C. (2005) Rapid purification of recombinant listeriolysin O (LLO) from Escherichia coli. J Ind Microbiol Biotechnol 32(8): 355-63. PubMed ID: 16052337
209.  (INT) Chute, I. C.; Hu, Z.; Liu, X. Q. (1998) A topA intein in Pyrococcus furiosus and its relatedness to the r-gyr intein of Methanococcus jannaschii. Gene 210(1): 85-92. PubMed ID: 9524230
210.  (APP) (EPL) (REV) Clark, K. M.; van der Donk, W. A.; Lu, Y. (2009) Expressed protein ligation for metalloprotein design and engineering. Methods Enzymol 462: 97-115. PubMed ID: 19632471
211.  (APP) (EPL) Clark, K. M.; Yu, Y.; Marshall, N. M.; Sieracki, N. A.; Nilges, M. J.; Blackburn, N. J.; van der Donk, W. A.; Lu, Y. (2010) Transforming a blue copper into a red copper protein: engineering cysteine and homocysteine into the axial position of azurin using site-directed mutagenesis and expressed protein ligation. J Am Chem Soc 132(29): 10093-101. PubMed ID: 20608676
212.  (REL) Clarke, S. (1987) Propensity for spontaneous succinimide formation from aspartyl and asparaginyl residues in cellular proteins. Int J Pept Protein Res 30(6): 808-21. PubMed ID: 3440704
213.  (INT) Clarke, N. D. (1994) A proposed mechanism for the self-splicing of proteins. Proc Natl Acad Sci U S A 91(23): 11084-8. PubMed ID: 7972014
214.  (APP) (REL) Clayton, D.; Shapovalov, G.; Maurer, J. A.; Dougherty, D. A.; Lester, H. A.; Kochendoerfer, G. G. (2004) Total chemical synthesis and electrophysiological characterization of mechanosensitive channels from Escherichia coli and Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 101(14): 4764-9. Epub 2004 Mar 23.. PubMed ID: 15041744
215.  (INT) (REV) Clyman, J. (1995) Some microbes have splicing proteins. ASM News 61: 344-347.
216.  (INT) Cohen, G. N.; Barbe, V.; Flament, D.; Galperin, M.; Heilig, R.; Lecompte, O.; Poch, O.; Prieur, D.; Querellou, J.; Ripp, R.; Thierry, J. C.; Van der Oost, J.; Weissenbach, J.; Zivanovic, Y.; Forterre, P. (2003) An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi. Mol Microbiol. 47(6): 1495-512.. PubMed ID: 12622808
217.  (INT) Cole, S. T.; Brosch, R.; Parkhill, J.; Garnier, T.; Churcher, C.; Harris, D.; Gordon, S. V.; Eiglmeier, K.; Gas, S.; Barry, C. E., 3rd; Tekaia, F.; Badcock, K.; Basham, D.; Brown, D.; Chillingworth, T.; Connor, R.; Davies, R.; Devlin, K.; Feltwell, T.; Gentles, S.; Hamlin, N.; Holroyd, S.; Hornsby, T.; Jagels, K.; Krogh, A.; McLean, J.; Moule, S.; Murphy, L.; Oliver, K.; Osborne, J.; Quail, M. A.; Rajandream, M. A.; Rogers, J.; Rutter, S.; Seeger, K.; Skelton, J.; Squares, R.; Squares, S.; Sulston, J. E.; Taylor, K.; Whitehead, S.; Barrell, B. G. (1998) Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 393(6685): 537-44.. PubMed ID: 9634230
218.  (APP) (EPL) (REV) Cole, P. A.; Sondhi, D.; Kim, K. (1999) Chemical approaches to the study of protein tyrosine kinases and their implications for mechanism and inhibitor design. Pharmacol Ther 82(2-3): 219-29. PubMed ID: 10454199
219.  (APP) (EPL) (REV) Cole, P. A.; Courtney, A. D.; Shen, K.; Zhang, Z.; Qiao, Y.; Lu, W.; Williams, D. M. (2003) Chemical approaches to reversible protein phosphorylation. Acc Chem Res 36(6): 444-52. PubMed ID: 12809531
220.  (APP) (PUR) Collins, E. D.; Espinoza, A.; Le, L. T.; Kato, M. (2010) Cloning the human vitamin D receptor into the pTwin-1 expression vector. J Steroid Biochem Mol Biol 121(1-2): 121-3. PubMed ID: 20171280
221.  (INT) (REV) Colston, M. J.; Davis, E. O. (1994) The ins and outs of protein splicing elements. Mol Microbiol 12(3): 359-63. PubMed ID: 8065257
222.  (EN) (INT) (REV) Colston, M.J.; Davis, E.O. (1994B) Homologous recombination, DNA repair and Mycobacterial ; recA ; genes. In: Tuberculosis: pathogenesis, protection and control, ed. Bloom. (American Society for Microbiology Press, Washington, DC) pages 217-226.
223.  (REL) Concordet, J. P.; Lewis, K. E.; Moore, J. W.; Goodrich, L. V.; Johnson, R. L.; Scott, M. P.; Ingham, P. W. (1996) Spatial regulation of a zebrafish patched homologue reflects the roles of sonic hedgehog and protein kinase A in neural tube and somite patterning. Development. 122(9): 2835-46.. PubMed ID: 8787757
224.  (APP) Contag, C. H.; Ross, B. D. (2002) It's not just about anatomy: In vivo bioluminescence imaging as an eyepiece into biology. Journal of Magnetic Resonance Imaging 16(4): 378-387.
225.  (APP) (CPS) (NEB) Cook, S.N.; Jack, W.E.; Xiong, X.; Danley, L.E.; Ellman, F.A.; Schultz, P.G.; Noren, C.J. (1995) Photochemically initiated protein splicing. Angew. Chem. Int. Ed. Engel. 34: 1629-1630.
226.  (INT) Cooper, A. A.; Chen, Y. J.; Lindorfer, M. A.; Stevens, T. H. (1993) Protein splicing of the yeast TFP1 intervening protein sequence: a model for self-excision. Embo J 12(6): 2575-83. PubMed ID: 8508780
227.  (INT) (REV) Cooper, A. A.; Stevens, T. H. (1993B) Protein splicing: excision of intervening sequences at the protein level. Bioessays 15(10): 667-74. PubMed ID: 8274142
228.  (INT) (REV) Cooper, A. A.; Stevens, T. H. (1995) Protein splicing: self-splicing of genetically mobile elements at the protein level. Trends Biochem Sci 20(9): 351-6. PubMed ID: 7482702
229.  (APP) Cottingham, I. R.; Millar, A.; Emslie, E.; Colman, A.; Schnieke, A. E.; McKee, C. (2001) A method for the amidation of recombinant peptides expressed as intein fusion proteins in Escherichia coli. Nat Biotechnol 19(10): 974-7. PubMed ID: 11581666
230.  (APP) (EPL) (TAG) Cotton, G. J.; Ayers, B.; Xu, R.; Muir, T. W. (1999) Insertion of a Synthetic Peptide into a Recombinant Protein Framework: A Protein Biosensor. J. Am. Chem. Soc. 121: 1100-1101.
231.  (APP) (EPL) (REV) Cotton, G. J.; Muir, T. W. (1999B) Peptide ligation and its application to protein engineering. Chem Biol. 6(9): R247-56.. PubMed ID: 10467135
232.  (APP) (EPL) (TAG) Cotton, G. J.; Muir, T. W. (2000) Generation of a dual-labeled fluorescence biosensor for Crk-II phosphorylation using solid-phase expressed protein ligation. Chem Biol 7(4): 253-61. PubMed ID: 10780925
233.  (APP) (EPL) (NMR) (REV) (Structure) Cowburn, D.; Muir, T. W. (2001) Segmental isotopic labeling using expressed protein ligation. Methods Enzymol 339: 41-54. PubMed ID: 11462824
234.  (APP) (EPL) (NMR) (REV) (Structure) Cowburn, D.; Shekhtman, A.; Xu, R.; Ottesen, J. J.; Muir, T. W. (2004) Segmental isotopic labeling for structural biological applications of NMR. Methods Mol Biol. 278: 47-56.. PubMed ID: 15317990
235.  (CYCL) (PUR) (REV) Craik, D. J.; Cemazar, M.; Daly, N. L. (2007) The chemistry and biology of cyclotides. Curr Opin Drug Discov Devel 10(2): 176-84. PubMed ID: 17436553
236.  (REL) (REV) Cresswell, P. (2004) Cell biology. Cutting and pasting antigenic peptides. Science 304(5670): 525-7. PubMed ID: 15105483
237.  (INT) (REL) Crona, M.; Moffatt, C.; Friedrich, N. C.; Hofer, A.; Sjoberg, B. M.; Edgell, D. R. (2010) Assembly of a fragmented ribonucleotide reductase by protein interaction domains derived from a mobile genetic element. Nucleic Acids Res (IN PRESS). PubMed ID: 20972217
238.  (APP) (EPL) (REV) Cuccia, Louis (2002) A chimeric enzyme with a [beta]-peptide reverse-turn. Trends in Biochemical Sciences 27(8): 391.
239.  (REL) (Structure) Cui, T.; Liao, P. H.; Guan, C.; Guo, H. C. (1999) Purification and crystallization of precursors and autoprocessed enzymes of Flavobacterium glycosylasparaginase: an N-terminal nucleophile hydrolase. Acta Crystallogr D Biol Crystallogr. 55(11): 1961-4.. PubMed ID: 10531509
240.  (APP) (PUR) Cui, C.; Zhao, W.; Chen, J.; Wang, J.; Li, Q. (2006) Elimination of in vivo cleavage between target protein and intein in the intein-mediated protein purification systems. Protein Expr Purif 50(1): 74-81. PubMed ID: 16884922
241.  (INT) Culley, A. I.; Asuncion, B. F.; Steward, G. F. (2009) Detection of inteins among diverse DNA polymerase genes of uncultivated members of the Phycodnaviridae. ISME J 3(4): 409-18. PubMed ID: 19079065
242.  (INT) (REV) Dalgaard, J. Z. (1994) Mobile introns and inteins: friend or foe?. Trends Genet 10(9): 306-7. PubMed ID: 7974743
243.  (EN) Dalgaard, J. Z.; Garrett, R. A.; Belfort, M. (1994) Purification and characterization of two forms of I-DmoI, a thermophilic site-specific endonuclease encoded by an archaeal intron. J Biol Chem 269(46): 28885-92. PubMed ID: 7961849
244.  (INT) Dalgaard, J. Z.; Moser, M. J.; Hughey, R.; Mian, I. S. (1997) Statistical modeling, phylogenetic analysis and structure prediction of a protein splicing domain common to inteins and hedgehog proteins. J Comput Biol 4(2): 193-214. PubMed ID: 9228618
245.  (EN) (INT) Dalgaard, J. Z.; Klar, A. J.; Moser, M. J.; Holley, W. R.; Chatterjee, A.; Mian, I. S. (1997B) Statistical modeling and analysis of the LAGLIDADG family of site-specific endonucleases and identification of an intein that encodes a site-specific endonuclease of the HNH family. Nucleic Acids Res 25(22): 4626-38. PubMed ID: 9358175
246.  (INT) (REL) Dassa, B.; Haviv, H.; Amitai, G.; Pietrokovski, S. (2004) Protein splicing and auto-cleavage of bacterial intein-like domains lacking a C'-flanking nucleophilic residue. J Biol Chem 279(31): 32001-7. PubMed ID: 15150275
247.  (REL) (REV) Dassa, B.; Yanai, I.; Pietrokovski, S. (2004B) New type of polyubiquitin-like genes with intein-like autoprocessing domains. Trends Genet 20(11): 538-42. PubMed ID: 15475112
248.  (INT) (REV) Dassa, B.; Pietrokovski, S. (2005) Origin and Evolution of Inteins and Other Hint Domains. In: Homing Endonucleases and Inteins, ed. Belfort, M.; Derbyshire, V.; Stoddard, B.L.; Wood, D.W.. (Springer-Verlag, Heidelberg, Germany) Vol. 16 pages 211-232.
249.  (CPS) (INT) (TS) Dassa, B.; Amitai, G.; Caspi, J.; Schueler-Furman, O.; Pietrokovski, S. (2007) Trans protein splicing of cyanobacterial split inteins in endogenous and exogenous combinations. Biochemistry 46(1): 322-30. PubMed ID: 17198403
250.  (INT) (REL) Dassa, B.; London, N.; Stoddard, B. L.; Schueler-Furman, O.; Pietrokovski, S. (2009) Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family. Nucleic Acids Res 37(8): 2560-73. PubMed ID: 19264795
251.  (INT) (REL) (Structure) Datta, S.; Prabu, M. M.; Vaze, M. B.; Ganesh, N.; Chandra, N. R.; Muniyappa, K.; Vijayan, M. (2000) Crystal structures of Mycobacterium tuberculosis RecA and its complex with ADP-AlF(4): implications for decreased ATPase activity and molecular aggregation. Nucleic Acids Res 28(24): 4964-73. PubMed ID: 11121488
252.  (APP) (PUR) Daugelat, S.; Jacobs, W. R., Jr. (1999) The Mycobacterium tuberculosis recA intein can be used in an ORFTRAP to select for open reading frames. Protein Sci 8(3): 644-53. PubMed ID: 10091667
253.  (APP) (EPL) (TAG) David, R.; Machova, Z.; Beck-Sickinger, A. G. (2003) Semisynthesis and application of carboxyfluorescein-labelled biologically active human interleukin-8. Biol Chem 384(12): 1619-30. PubMed ID: 14719805
254.  (APP) (REV) David, R.; Richter, M. P.; Beck-Sickinger, A. G. (2004) Expressed protein ligation. Method and applications. Eur J Biochem 271(4): 663-77. PubMed ID: 14764082
255.  (APP) (EPL) David, R.; Beck-Sickinger, A. G. (2007) Identification of the dimerisation interface of human interleukin-8 by IL-8-variants containing the photoactivatable amino acid benzoyl-phenylalanine. Eur Biophys J 36(4-5): 385-391. PubMed ID: 17024463
256.  (APP) (PUR) (Structure) Davies, D. R.; Goryshin, I. Y.; Reznikoff, W. S.; Rayment, I. (2000) Three-dimensional structure of the Tn5 synaptic complex transposition intermediate. Science 289(5476): 77-85. PubMed ID: 10884228
257.  (INT) Davis, E. O.; Sedgwick, S. G.; Colston, M. J. (1991) Novel structure of the recA locus of Mycobacterium tuberculosis implies processing of the gene product. J Bacteriol. 173(18): 5653-62.. PubMed ID: 1909321
258.  (INT) Davis, E. O.; Jenner, P. J.; Brooks, P. C.; Colston, M. J.; Sedgwick, S. G. (1992) Protein splicing in the maturation of M. tuberculosis recA protein: a mechanism for tolerating a novel class of intervening sequence. Cell 71(2): 201-10. PubMed ID: 1423588
259.  (INT) Davis, E. O.; Thangaraj, H. S.; Brooks, P. C.; Colston, M. J. (1994) Evidence of selection for protein introns in the recAs of pathogenic mycobacteria. Embo J 13(3): 699-703. PubMed ID: 7508863
260.  (APP) (EPL) (REV) Davis, E. O.; Jenner, P. J. (1995) Protein splicing--the lengths some proteins will go to. Antonie Van Leeuwenhoek 67(2): 131-7. PubMed ID: 7771761
261.  (APP) Davis, B. G. (2009) The linear assembly of a pure glycoenzyme. Angew Chem Int Ed Engl 48(26): 4674-8. PubMed ID: 19418504
262.  (REL) Dawson, P. E.; Muir, T. W.; Clark-Lewis, I.; Kent, S. B. (1994) Synthesis of proteins by native chemical ligation. Science. 266(5186): 776-9.. PubMed ID: 7973629
263.  (APP) (EPL) (REL) (REV) Dawson, P. E.; Kent, S. B. (2000) Synthesis of native proteins by chemical ligation. Annu Rev Biochem. 69: 923-60.. PubMed ID: 10966479
264.  (APP) (REV) de Grey, A. D. (2000) Mitochondrial gene therapy: an arena for the biomedical use of inteins. Trends Biotechnol 18(9): 394-9. PubMed ID: 10942964
265.  (REL) de Araujo, A. D.; Palomo, J. M.; Cramer, J.; Seitz, O.; Alexandrov, K.; Waldmann, H. (2006) Diels-Alder ligation of peptides and proteins. Chemistry 12(23): 6095-109. PubMed ID: 16807971
266.  (INT) Deckert, G.; Warren, P. V.; Gaasterland, T.; Young, W. G.; Lenox, A. L.; Graham, D. E.; Overbeek, R.; Snead, M. A.; Keller, M.; Aujay, M.; Huber, R.; Feldman, R. A.; Short, J. M.; Olsen, G. J.; Swanson, R. V. (1998) The complete genome of the hyperthermophilic bacterium Aquifex aeolicus. Nature. 392(6674): 353-8.. PubMed ID: 9537320
267.  (INT) Degnan, P. H.; Moran, N. A. (2008) Diverse phage-encoded toxins in a protective insect endosymbiont. Appl Environ Microbiol 74(21): 6782-91. PubMed ID: 18791000
268.  (INT) (REV) Demidov, V. V. (2001) Splice juntion function [newsinbrief]. Modern Drug Discovery 4: 16.
269.  (APP) (PUR) Demidov, V. V.; Broude, N. E. (2006) Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitro. Nat Protoc 1(2): 714-9. PubMed ID: 17406301
270.  (REL) Demirov, D.; Sarafian, V.; Kremensky, I.; Ganev, V. (1999) Evidence for protein splicing in the endoplasmic reticulum-Golgi intermediate compartment. Biochim Biophys Acta 1448(3): 507-11. PubMed ID: 9990302
271.  (APP) (PUR) Deng, Q.; Kong, Y. Y.; Xie, Y. H.; Wang, Y. (2005) Expression and purification of the complete PreS region of hepatitis B Virus. World J Gastroenterol 11(20): 3060-4. PubMed ID: 15918190
272.  (EN) (INT) Derbyshire, V.; Wood, D. W.; Wu, W.; Dansereau, J. T.; Dalgaard, J. Z.; Belfort, M. (1997) Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolution. Proc Natl Acad Sci U S A 94(21): 11466-71. PubMed ID: 9326633
273.  (INT) (REV) Derbyshire, V.; Belfort, M. (1998) Lightning strikes twice: intron-intein coincidence. Proc Natl Acad Sci U S A 95(4): 1356-7. PubMed ID: 9465018
274.  (EN) (INT) Derbyshire, V.; Wood, D. W.; Wu, W.; Dansereau, J. T.; Dalgaard, J. Z.; Belfort, M. (1998) Erratum: Genetic definition of a protein-splicing domain: Functional mini-inteins support structure predictions and a model for intein evolution (Proceedings of the National Academy of Sciences of the United States of America (October 14, 1997) 21:94 (11,466-11,471)). Proceedings of the National Academy of Sciences of the United States of America 95(2): 762.
275.  (INT) (REL) Derrien, B.; Majeran, W.; Wollman, F. A.; Vallon, O. (2009) Multistep processing of an insertion sequence in an essential subunit of the chloroplast ClpP complex. J Biol Chem 284(23): 15408-15. PubMed ID: 19346247
276.  (APP) (CYCL) (TS) Deschuyteneer, G.; Garcia, S.; Michiels, B.; Baudoux, B.; Degand, H.; Morsomme, P.; Soumillion, P. (2010) Intein-mediated cyclization of randomized peptides in the periplasm of Escherichia coli and their extracellular secretion. ACS Chem Biol 5(7): 691-700. PubMed ID: 20527881
277.  (APP) (PUR) Di, L.; Zhang, H. W.; Xu, L. L. (2006) [Use of Ssp dnaB mini-intein as a fusion partner for preparation of recombinant human brain natriuretic peptide]. Sheng Wu Gong Cheng Xue Bao. 22(2): 180-6, 197, 203 passim. PubMed ID: 16607940
278.  (APP) (INT) (REV) (Structure) Di, L.; Zhang, H. W.; Xu, L. L. (2007) Progress in the study of intein. Pharmaceutical Biotechnology 14(6): 445-450.
279.  (APP) (PUR) Diao, H.; Guo, C.; Lin, D.; Zhang, Y. (2007) Intein-mediated expression is an effective approach in the study of beta-defensins. Biochem Biophys Res Commun 357(4): 840-846. PubMed ID: 17445764
280.  (INT) (NEB) (Structure) Ding, Y.; Xu, M. Q.; Ghosh, I.; Chen, X.; Ferrandon, S.; Lesage, G.; Rao, Z. (2003) Crystal structure of a mini-intein reveals a conserved catalytic module involved in side chain cyclization of asparagine during protein splicing. J Biol Chem 278(40): 39133-42. PubMed ID: 12878593
281.  (APP) (PUR) Ding, Y.; Jiang, W.; Su, Y.; Zhou, H.; Zhang, Z. (2004) Expression and purification of recombinant cytoplasmic domain of human erythrocyte band 3 with hexahistidine tag or chitin-binding tag in Escherichia coli. Protein Expr Purif 34(2): 167-75. PubMed ID: 15003247
282.  (APP) (PUR) Ding, F. X.; Yan, H. L.; Mei, Q.; Xue, G.; Wang, Y. Z.; Gao, Y. J.; Sun, S. H. (2007) A novel, cheap and effective fusion expression system for the production of recombinant proteins. Appl Microbiol Biotechnol 77(2): 483-8. PubMed ID: 17768617
283.  (INT) (REV) Doerr, A. (2005) Frankenstein's protein. Nature Methods 2: 408.
284.  (EN) (REV) Doolittle, R. F. (1993) The comings and goings of homing endonucleases and mobile introns. Proc Natl Acad Sci U S A. 90(12): 5379-81.. PubMed ID: 8390659
285.  (INT) (REV) Doolittle, W. F.; Stoltzfus, A. (1993B) Molecular evolution. Genes-in-pieces revisited. Nature. 361(6411): 403.. PubMed ID: 8429878
286.  (REL) Dori-Bachash, M.; Dassa, B.; Peleg, O.; Pineiro, S. A.; Jurkevitch, E.; Pietrokovski, S. (2009) Bacterial intein-like domains of predatory bacteria: a new domain type characterized in Bdellovibrio bacteriovorus. Funct Integr Genomics 9(2): 153-66. PubMed ID: 19153786
287.  (APP) (PUR) Dorsch, S.; Kaufmann, B.; Schaible, U.; Prohaska, E.; Wolf, H.; Modrow, S. (2001) The VP1-unique region of parvovirus B19: amino acid variability and antigenic stability. J Gen Virol 82(Pt 1): 191-9. PubMed ID: 11125172
288.  (INT) Dorval, Deirdre M.; Connor, Katherine R.; Mills, Kenneth V. (2006) Catalysis of individual steps of protein splicing of the Pyrococcus abyssi PolII intein. FASEB J._MeetingAbstracts 20(4): A40-d.
289.  (APP) (EPL) (NMR) (REV) (Structure) Dotsch, V.; Wagner, G. (1998) New approaches to structure determination by NMR spectroscopy. Curr Opin Struct Biol. 8(5): 619-23.. PubMed ID: 9818267
290.  (INT) Douglas, S. E.; Penny, S. L. (1999) The plastid genome of the cryptophyte alga, Guillardia theta: complete sequence and conserved synteny groups confirm its common ancestry with red algae. J Mol Evol. 48(2): 236-44.. PubMed ID: 9929392
291.  (APP) (REV) Doyle, D. F.; Corey, D. R. (1998) Engineering proteins: keeping up with biology. Chem Biol. 5(7): R157-60.. PubMed ID: 9662511
292.  (EN) Drouin, M.; Lucas, P.; Otis, C.; Lemieux, C.; Turmel, M. (2000) Biochemical characterization of I-CmoeI reveals that this H-N-H homing endonuclease shares functional similarities with H-N-H colicins. Nucleic Acids Res. 28(22): 4566-72.. PubMed ID: 11071947
293.  (INT) (NMR) Du, Z.; Liu, Y.; Zheng, Y.; McCallum, S.; Dansereau, J.; Derbyshire, V.; Belfort, M.; Belfort, G.; Van Roey, P.; Wang, C. (2008) 1H, 13C, and 15N NMR assignments of an engineered intein based on Mycobacterium tuberculosis RecA. Biomol NMR Assign 2(2): 111-3. PubMed ID: 19636882
294.  (INT) (NMR) Du, Z.; Shemella, P. T.; Liu, Y.; McCallum, S. A.; Pereira, B.; Nayak, S. K.; Belfort, G.; Belfort, M.; Wang, C. (2009) Highly conserved histidine plays a dual catalytic role in protein splicing: a pKa shift mechanism. J Am Chem Soc 131(32): 11581-9. PubMed ID: 19630416
295.  (INT) (NMR) (Structure) Du, Z.; Liu, Y.; Ban, D.; Lopez, M. M.; Belfort, M.; Wang, C. (2010) Backbone dynamics and global effects of an activating mutation in minimized Mtu RecA inteins. J Mol Biol 400(4): 755-67. PubMed ID: 20562025
296.  (EN) (INT) (Structure) Duan, X.; Gimble, F. S.; Quiocho, F. A. (1997) Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity. Cell 89(4): 555-64. PubMed ID: 9160747
297.  (INT) Duffee, Lauren R.; York, Daniel J.; Dorval, Deirdre M.; Reitter, Julie N.; Mills, Kenneth V. (2010) A kinetic analysis of each step of protein splicing of the Pyrococcus abyssi PolII intein. FASEB J. 24(1_MeetingAbstracts): 463.5-.
298.  (EN) (INT) (REL) Dujon, B.; Sherman, D.; Fischer, G.; Durrens, P.; Casaregola, S.; Lafontaine, I.; De Montigny, J.; Marck, C.; Neuveglise, C.; Talla, E.; Goffard, N.; Frangeul, L.; Aigle, M.; Anthouard, V.; Babour, A.; Barbe, V.; Barnay, S.; Blanchin, S.; Beckerich, J. M.; Beyne, E.; Bleykasten, C.; Boisrame, A.; Boyer, J.; Cattolico, L.; Confanioleri, F.; De Daruvar, A.; Despons, L.; Fabre, E.; Fairhead, C.; Ferry-Dumazet, H.; Groppi, A.; Hantraye, F.; Hennequin, C.; Jauniaux, N.; Joyet, P.; Kachouri, R.; Kerrest, A.; Koszul, R.; Lemaire, M.; Lesur, I.; Ma, L.; Muller, H.; Nicaud, J. M.; Nikolski, M.; Oztas, S.; Ozier-Kalogeropoulos, O.; Pellenz, S.; Potier, S.; Richard, G. F.; Straub, M. L.; Suleau, A.; Swennen, D.; Tekaia, F.; Wesolowski-Louvel, M.; Westhof, E.; Wirth, B.; Zeniou-Meyer, M.; Zivanovic, I.; Bolotin-Fukuhara, M.; Thierry, A.; Bouchier, C.; Caudron, B.; Scarpelli, C.; Gaillardin, C.; Weissenbach, J.; Wincker, P.; Souciet, J. L. (2004) Genome evolution in yeasts. Nature. 430(6995): 35-44.. PubMed ID: 15229592
299.  (EN) (REV) Dujon, B. (2005) Homing Endonucleases and the Yeast Mitochondrial ; w; Locus - A Historical Perspective. In: Homing Endonucleases and Inteins, ed. Belfort, M.; Derbyshire, V.; Stoddard, B.L.; Wood, D.W.. (Springer-Verlag, Heidelberg, Germany) Vol. 16 pages 11-32.
300.  (APP) (NEB) (TS) Dun, B. Q.; Lu, W.; Wang, X. J.; Chen, M.; Jin, D.; Zhao, Z. L.; Xu, M. Q.; Lin, M. (2006) Reconstruction of enzymatic activity from split genes encoding glyphosate-tolerant EPSPS protein from Psedomonas fluorescens G2 strain by intein mediated protein complementtion. Chinese Science Bulletin 51(13): 1652-1654.
301.  (APP) (TS) Dun, B. Q.; Wang, X. J.; Lu, W.; Zhao, Z. L.; Hou, S. N.; Zhang, B. M.; Li, G. Y.; Evans, T. C., Jr.; Xu, M. Q.; Lin, M. (2007) Reconstitution of glyphosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco. Appl Environ Microbiol 73(24): 7997-8000. PubMed ID: 17951442
302.  (APP) (EPL) Durek, T.; Alexandrov, K.; Goody, R. S.; Hildebrand, A.; Heinemann, I.; Waldmann, H. (2004B) Synthesis of fluorescently labeled mono- and diprenylated Rab7 GTPase. J Am Chem Soc 126(50): 16368-78. PubMed ID: 15600338
303.  (APP) (EPL) (REV) Durek, T.; Goody, R. S.; Alexandrov, K. (2004C) In vitro semisynthesis and applications of C-terminally modified rab proteins. Methods Mol Biol 283: 233-44. PubMed ID: 15197315
304.  (APP) (EPL) (REV) Durek, T.; Becker, C. F. (2005) Protein semi-synthesis: new proteins for functional and structural studies. Biomol Eng 22(5-6): 153-72. PubMed ID: 16188500
305.  (REL) (Structure) Durek, T.; Torbeev, V. Y.; Kent, S. B. (2007) Convergent chemical synthesis and high-resolution x-ray structure of human lysozyme. Proc Natl Acad Sci U S A 104(12): 4846-51. PubMed ID: 17360367
306.  (APP) (REL) Dwyer, M. A.; Lu, W.; Dwyer, J. J.; Kossiakoff, A. A. (2000) Biosynthetic phage display: a novel protein engineering tool combining chemical and genetic diversity. Chem Biol. 7(4): 263-74.. PubMed ID: 10780926
307.  (APP) (EPL) Eckenroth, B.; Harris, K.; Turanov, A. A.; Gladyshev, V. N.; Raines, R. T; Hondal, R. J. (2006) Semisynthesis and Characterization of Mammalian Thioredoxin Reductase. Biochemistry 45(16): 5158-5170. PubMed ID: 16618105
308.  (EN) (REL) Eddy, S. R.; Gold, L. (1992) Artificial mobile DNA element constructed from the EcoRI endonuclease gene. Proc Natl Acad Sci U S A. 89(5): 1544-7.. PubMed ID: 1311841
309.  (INT) (REV) Edgell, D. R.; Fast, N. M.; Doolittle, W. F. (1996) Selfish DNA: the best defense is a good offense. Curr Biol. 6(4): 385-8.. PubMed ID: 8723339
310.  (REL) (REV) Edgell, D. R.; Belfort, M.; Shub, D. A. (2000) Barriers to intron promiscuity in bacteria. J Bacteriol 182(19): 5281-9. PubMed ID: 10986228
311.  (EN) (REV) Edgell, D. R. (2002) Selfish DNA: new abode for homing endonucleases. Curr Biol. 12(8): R276-8.. PubMed ID: 11967166
312.  (EN) Edgell, D. R.; Stanger, M. J.; Belfort, M. (2004) Coincidence of cleavage sites of intron endonuclease I-TevI and critical sequences of the host thymidylate synthase gene. J Mol Biol 343(5): 1231-41. PubMed ID: 15491609
313.  (INT) Eichinger, L.; Pachebat, J. A.; Glockner, G.; Rajandream, M. A.; Sucgang, R.; Berriman, M.; Song, J.; Olsen, R.; Szafranski, K.; Xu, Q.; Tunggal, B.; Kummerfeld, S.; Madera, M.; Konfortov, B. A.; Rivero, F.; Bankier, A. T.; Lehmann, R.; Hamlin, N.; Davies, R.; Gaudet, P.; Fey, P.; Pilcher, K.; Chen, G.; Saunders, D.; Sodergren, E.; Davis, P.; Kerhornou, A.; Nie, X.; Hall, N.; Anjard, C.; Hemphill, L.; Bason, N.; Farbrother, P.; Desany, B.; Just, E.; Morio, T.; Rost, R.; Churcher, C.; Cooper, J.; Haydock, S.; van Driessche, N.; Cronin, A.; Goodhead, I.; Muzny, D.; Mourier, T.; Pain, A.; Lu, M.; Harper, D.; Lindsay, R.; Hauser, H.; James, K.; Quiles, M.; Madan Babu, M.; Saito, T.; Buchrieser, C.; Wardroper, A.; Felder, M.; Thangavelu, M.; Johnson, D.; Knights, A.; Loulseged, H.; Mungall, K.; Oliver, K.; Price, C.; Quail, M. A.; Urushihara, H.; Hernandez, J.; Rabbinowitsch, E.; Steffen, D.; Sanders, M.; Ma, J.; Kohara, Y.; Sharp, S.; Simmonds, M.; Spiegler, S.; Tivey, A.; Sugano, S.; White, B.; Walker, D.; Woodward, J.; Winckler, T.; Tanaka, Y.; Shaulsky, G.; Schleicher, M.; Weinstock, G.; Rosenthal, A.; Cox, E. C.; Chisholm, R. L.; Gibbs, R.; Loomis, W. F.; Platzer, M.; Kay, R. R.; Williams, J.; Dear, P. H.; Noegel, A. A.; Barrell, B.; Kuspa, A. (2005) The genome of the social amoeba Dictyostelium discoideum. Nature. 435(7038): 43-57.. PubMed ID: 15875012
314.  (INT) Eiglmeier, K.; Honore, N.; Woods, S. A.; Caudron, B.; Cole, S. T. (1993) Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae. Mol Microbiol. 7(2): 197-206.. PubMed ID: 8446027
315.  (REL) (Structure) Ekstrom, J. L.; Tolbert, W. D.; Xiong, H.; Pegg, A. E.; Ealick, S. E. (2001) Structure of a human S-adenosylmethionine decarboxylase self-processing ester intermediate and mechanism of putrescine stimulation of processing as revealed by the H243A mutant. Biochemistry 40(32): 9495-504. PubMed ID: 11583148
316.  (APP) (EPL) (TAG) Elias, D. R.; Cheng, Z.; Tsourkas, A. (2010) An intein-mediated site-specific click conjugation strategy for improved tumor targeting of nanoparticle systems. Small 6(21): 2460-8. PubMed ID: 20925038
317.  (INT) Elleuche, S.; Nolting, N.; Poggeler, S. (2006) Protein splicing of PRP8 mini-inteins from species of the genus Penicillium. Appl Microbiol Biotechnol 72(5): 959-67. PubMed ID: 16544141
318.  (APP) (TS) Elleuche, S.; Poggeler, S. (2007) Trans-splicing of an artificially split fungal mini-intein. Biochem Biophys Res Commun 355: 830-834. PubMed ID: 17316565
319.  (INT) Elleuche, S.; Doring, K.; Poggeler, S. (2008) Minimization of a eukaryotic mini-intein. Biochem Biophys Res Commun 366(1): 239-43. PubMed ID: 18054328
320.  (INT) Elleuche, S.; Pelikan, C.; Nolting, N.; Poggeler, S. (2009) Inteins and introns within the prp8 -gene of four Eupenicillium species. J Basic Microbiol 49(1): 52-7. PubMed ID: 19253333
321.  (INT) (REV) Elleuche, S.; Poggeler, S. (2009) Inteins - Selfish Elements in Fungal Genomes. In: The Mycota XV. Physiology and Genetics. Selected Basic and Applied Aspects, ed. Anke, T.; Weber. D.. (Springer, Heidelberg, Berlin) pages 41-61.
322.  (APP) (INT) (REV) (TS) Elleuche, S.; Poggeler, S. (2010) Inteins, valuable genetic elements in molecular biology and biotechnology. Appl Microbiol Biotechnol 87(2): 479-89. PubMed ID: 20449740
323.  (APP) (REL) Elliott, R. J.; Bennet, A. J.; Braun, C. A.; MacLeod, A. M.; Borgford, T. J. (2000) Active-site variants of Streptomyces griseus protease B with peptide-ligation activity. Chem Biol. 7(3): 163-71.. PubMed ID: 10712933
324.  (REL) Engelhard, V. H. (2004) Creating new peptide antigens by slicing and splicing proteins. Nat Immunol 5(2): 128-9. PubMed ID: 14749782
325.  (INT) Erkel, C.; Kube, M.; Reinhardt, R.; Liesack, W. (2006) Genome of Rice Cluster I archaea--the key methane producers in the rice rhizosphere. Science 313(5785): 370-2. PubMed ID: 16857943
326.  (APP) (REL) Erlich, L. A.; Kumar, K. S.; Haj-Yahya, M.; Dawson, P. E.; Brik, A. (2010) N-methylcysteine-mediated total chemical synthesis of ubiquitin thioester. Org Biomol Chem 8(10): 2392-6. PubMed ID: 20448897
327.  (APP) (PUR) Esipov, R. S.; Gurevich, A. I.; Stepanenko, V. N.; Chupova, L. A.; Chuvikovskii, D. V.; Miroshnikov, A. I. (2004) [Recombinant thymosin alpha1]. Bioorg Khim 30(5): 481-6. PubMed ID: 15562968
328.  (APP) (PUR) Esipov, R. S.; Stepanenko, V. N.; Gurevich, A. I.; Chupova, L. A.; Miroshnikov, A. I. (2006) Production and purification of recombinant human glucagon overexpressed as intein fusion protein in Escherichia coli. Protein Pept Lett. 13(4): 343-7. PubMed ID: 16712508
329.  (APP) (PUR) Esipov, R. S.; Stepanenko, V. N.; Chupova, L. A.; Boyarskikh, U. A.; Filipenko, M. L.; Miroshnikov, A. I. (2008) Production of recombinant human epidermal growth factor using Ssp dnaB mini-intein system. Protein Expr Purif 61(1): 1-6. PubMed ID: 18599312
330.  (APP) (EPL) (TAG) Esser-Kahn, A. P.; Francis, M. B. (2008) Protein-cross-linked polymeric materials through site-selective bioconjugation. Angew Chem Int Ed Engl 47(20): 3751-4. PubMed ID: 18399554
331.  (APP) (CPS) (REV) Evanko, D. (2007) Controlling proteins the intein way. Nat Methods 4(2): 112-3. PubMed ID: 17326269
332.  (EPL) (NEB) (PUR) Evans, T. C., Jr.; Benner, J.; Xu, M. Q. (1998) Semisynthesis of cytotoxic proteins using a modified protein splicing element. Protein Sci 7(11): 2256-64. PubMed ID: 9827992
333.  (APP) (EPL) (NEB) (REV) Evans, T. C.; Xu, M.-Q. (1998B) Intein Mediated Protein Ligation (IPL). The NEB Transcript 9(1): 10-11.
334.  (APP) (EPL) (INT) (NEB) Evans, T. C., Jr.; Benner, J.; Xu, M. Q. (1999) The in vitro ligation of bacterially expressed proteins using an intein from Methanobacterium thermoautotrophicum. J Biol Chem 274(7): 3923-6. PubMed ID: 9933578
335.  (APP) (CYCL) (NEB) Evans, T. C., Jr.; Benner, J.; Xu, M. Q. (1999B) The cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins. J Biol Chem 274(26): 18359-63. PubMed ID: 10373440
336.  (APP) (EPL) (NEB) (REV) Evans, T. C., Jr.; Xu, M. Q. (1999C) Intein-mediated protein ligation: harnessing nature's escape artists. Biopolymers 51(5): 333-42. PubMed ID: 10685044
337.  (APP) (CYCL) (EPL) (INT) (NEB) Evans, T. C., Jr.; Martin, D.; Kolly, R.; Panne, D.; Sun, L.; Ghosh, I.; Chen, L.; Benner, J.; Liu, X. Q.; Xu, M. Q. (2000) Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803. J Biol Chem 275(13): 9091-4. PubMed ID: 10734038
338.  (INT) (NEB) (REV) Evans, Tc Jr Tc; Xu, M. Q. (2002) Mechanistic and kinetic considerations of protein splicing. Chem Rev 102(12): 4869-84. PubMed ID: 12475209
339.  (APP) (NEB) (REV) (TS) Evans, T. C., Jr.; Xu, M. Q.; Pradhan, S. (2005) Protein splicing elements and plants: from transgene containment to protein purification. Annu Rev Plant Biol 56: 375-92. PubMed ID: 15862101
340.  (APP) (EPL) (TAG) Evans, J. (2005) Tadpoles stick to protein analysis. Chemistry World 2(2): 18.
341.  (INT) Falb, M.; Pfeiffer, F.; Palm, P.; Rodewald, K.; Hickmann, V.; Tittor, J.; Oesterhelt, D. (2005) Living with two extremes: conclusions from the genome sequence of Natronomonas pharaonis. Genome Res. 15(10): 1336-43. Epub 2005 Sep 16.. PubMed ID: 16169924
342.  (APP) (PUR) Fan, X.; Shi, H.; Adelman, K.; Lis, J. T. (2004) Probing TBP interactions in transcription initiation and reinitiation with RNA aptamers that act in distinct modes. Proc Natl Acad Sci U S A 101(18): 6934-9. PubMed ID: 15103022
343.  (APP) (PUR) Feng, X. Q.; Wang, X. N.; Ning, Y. S.; Zhou, S. Y. (2002) [Construction of the recombinant plasmid FasAD-pTYB2 capable of Fas activation domain fusion expression]. Di Yi Jun Yi Da Xue Xue Bao 22(3): 227-30. PubMed ID: 12390771
344.  (INT) Fischer, M. G.; Allen, M. J.; Wilson, W. H.; Suttle, C. A. (2010) Giant virus with a remarkable complement of genes infects marine zooplankton. Proc Natl Acad Sci U S A (IN PRESS). PubMed ID: 20974979
345.  (INT) Fitzgerald, L. A.; Graves, M. V.; Li, X.; Feldblyum, T.; Nierman, W. C.; Van Etten, J. L. (2007) Sequence and annotation of the 369-kb NY-2A and the 345-kb AR158 viruses that infect Chlorella NC64A. Virology 358(2): 472-84. PubMed ID: 17027058
346.  (EN) (INT) (NEB) Fitzsimons Hall, M.; Noren, C. J.; Perler, F. B.; Schildkraut, I. (2002) Creation of an artificial bifunctional intein by grafting a homing endonuclease into a mini-intein. J Mol Biol 323(2): 173-9. PubMed ID: 12381313
347.  (APP) (REL) Flavell, R. R.; Huse, M.; Goger, M.; Trester-Zedlitz, M.; Kuriyan, J.; Muir, T. W. (2002) Efficient semisynthesis of a tetraphosphorylated analogue of the Type I TGFbeta receptor. Org Lett. 4(2): 165-8.. PubMed ID: 11796041
348.  (APP) (EPL) Flavell, R. R.; Kothari, P.; Bar-Dagan, M.; Synan, M.; Vallabhajosula, S.; Friedman, J. M.; Muir, T. W.; Ceccarini, G. (2008) Site-specific (18)F-labeling of the protein hormone leptin using a general two-step ligation procedure. J Am Chem Soc 130(28): 9106-12. PubMed ID: 18570424
349.  (APP) (EPL) (REV) Flavell, R. R.; Muir, T. W. (2009) Expressed protein ligation (EPL) in the study of signal transduction, ion conduction, and chromatin biology. Acc Chem Res 42(1): 107-16. PubMed ID: 18939858
350.  (INT) Fleischmann, R. D.; Alland, D.; Eisen, J. A.; Carpenter, L.; White, O.; Peterson, J.; DeBoy, R.; Dodson, R.; Gwinn, M.; Haft, D.; Hickey, E.; Kolonay, J. F.; Nelson, W. C.; Umayam, L. A.; Ermolaeva, M.; Salzberg, S. L.; Delcher, A.; Utterback, T.; Weidman, J.; Khouri, H.; Gill, J.; Mikula, A.; Bishai, W.; Jacobs Jr, W. R., Jr.; Venter, J. C.; Fraser, C. M. (2002) Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains. J Bacteriol. 184(19): 5479-90.. PubMed ID: 12218036
351.  (EN) Flick, K. E.; Jurica, M. S.; Monnat, R. J., Jr.; Stoddard, B. L. (1998) DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI. Nature 394(6688): 96-101. PubMed ID: 9665136
352.  (APP) (PUR) (REL) Floss, D. M.; Sack, M.; Arcalis, E.; Stadlmann, J.; Quendler, H.; Rademacher, T.; Stoger, E.; Scheller, J.; Fischer, R.; Conrad, U. (2009) Influence of elastin-like peptide fusions on the quantity and quality of a tobacco-derived human immunodeficiency virus-neutralizing antibody. Plant Biotechnol J 7(9): 899-913. PubMed ID: 19843249
353.  (APP) (PUR) (REL) (REV) Floss, D. M.; Schallau, K.; Rose-John, S.; Conrad, U.; Scheller, J. (2010) Elastin-like polypeptides revolutionize recombinant protein expression and their biomedical application. Trends Biotechnol 28(1): 37-45. PubMed ID: 19897265
354.  (APP) (EPL) Focke, P. J.; Valiyaveetil, F. I. (2010) Studies of ion channels using expressed protein ligation. Curr Opin Chem Biol (IN PRESS). PubMed ID: 20965773
355.  (APP) (PUR) Fong, B. A.; Wu, W. Y.; Wood, D. W. (2009) Optimization of ELP-intein mediated protein purification by salt substitution. Protein Expr Purif 66(2): 198-202. PubMed ID: 19345265
356.  (APP) (PUR) (REV) Fong, B. A.; Wu, W. Y.; Wood, D. W. (2010) The potential role of self-cleaving purification tags in commercial-scale processes. Trends Biotechnol 28(5): 272-9. PubMed ID: 20359761
357.  (APP) (PUR) Fong, B. A.; Wood, D. W. (2010) Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation. Microb Cell Fact 9(1): 77. PubMed ID: 20959011
358.  (APP) (PUR) Fong, B. A.; Gillies, A. R.; Ghazi, I.; LeRoy, G.; Lee, K. C.; Westblade, L. F.; Wood, D. W. (2010B) Purification of Escherichia coli RNA polymerase using a self-cleaving elastin-like polypeptide tag. Protein Sci 19(6): 1243-52. PubMed ID: 20512976
359.  (APP) (REV) Frey, D. D.; Kang, X. (2005) New concepts in the chromatography of peptides and proteins. Curr Opin Biotechnol 16(5): 552-60. PubMed ID: 16081277
360.  (REL) Friedrich, N. C.; Torrents, E.; Gibb, E. A.; Sahlin, M.; Sjoberg, B. M.; Edgell, D. R. (2007) Insertion of a homing endonuclease creates a genes-in-pieces ribonucleotide reductase that retains function. Proc Natl Acad Sci U S A 104(15): 6176-81. PubMed ID: 17395719
361.  (INT) Frischkorn, K.; Sander, P.; Scholz, M.; Teschner, K.; Prammananan, T.; Bottger, E. C. (1998) Investigation of mycobacterial recA function: protein introns in the RecA of pathogenic mycobacteria do not affect competency for homologous recombination. Mol Microbiol 29(5): 1203-14. PubMed ID: 9767588
362.  (INT) Frischkorn, K.; Springer, B.; Bottger, E. C.; Davis, E. O.; Colston, M. J.; Sander, P. (2000) In vivo splicing and functional characterization of Mycobacterium leprae RecA. J Bacteriol 182(12): 3590-2. PubMed ID: 10852894
363.  (EPL) (INT) (NMR) (Structure) Frutos, S.; Goger, M.; Giovani, B.; Cowburn, D.; Muir, T. W. (2010) Branched intermediate formation stimulates peptide bond cleavage in protein splicing. Nat Chem Biol 6(7): 527-33. PubMed ID: 20495572
364.  (EN) (INT) Fsihi, H.; Vincent, V.; Cole, S. T. (1996) Homing events in the gyrA gene of some mycobacteria. Proc Natl Acad Sci U S A 93(8): 3410-5. PubMed ID: 8622949
365.  (INT) Fsihi, H.; De Rossi, E.; Salazar, L.; Cantoni, R.; Labo, M.; Riccardi, G.; Takiff, H. E.; Eiglmeier, K.; Bergh, S.; Cole, S. T. (1996B) Gene arrangement and organization in a approximately 76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium leprae. Microbiology 142 ( Pt 11): 3147-61. PubMed ID: 8969512
366.  (EN) (INT) Fukuda, T.; Nogami, S.; Ohya, Y. (2003) VDE-initiated intein homing in Saccharomyces cerevisiae proceeds in a meiotic recombination-like manner. Genes Cells 8(7): 587-602. PubMed ID: 12839620
367.  (EN) (INT) (REV) Fukuda, T.; Nagai, Y.; Ohya, Y. (2004) Molecular mechanism of VDE-initiated intein homing in yeast nuclear genome. Adv Biophys 38: 215-32. PubMed ID: 15493335
368.  (EN) (INT) Fukuda, T.; Ohta, K.; Ohya, Y. (2006) Investigation of the Mechanism of Meiotic DNA Cleavage by VMA1-Derived Endonuclease Uncovers a Meiotic Alteration in Chromatin Structure around the Target Site. Eukaryot Cell. 5(6): 981-90. PubMed ID: 16757746
369.  (EN) Fukuda, T.; Ohya, Y. (2006) Recruitment of RecA homologs Dmc1p and Rad51p to the double-strand break repair site initiated by meiosis-specific endonuclease VDE (PI-SceI). Mol Genet Genomics 275(2): 204-14. PubMed ID: 16341884
370.  (APP) (EN) (REL) Fukuda, T.; Ohya, Y.; Ohta, K. (2007) Conditional genomic rearrangement by designed meiotic recombination using VDE (PI-SceI) in yeast. Mol Genet Genomics 278(4): 467-78. PubMed ID: 17587055
371.  (INT) Fukui, T.; Atomi, H.; Kanai, T.; Matsumi, R.; Fujiwara, S.; Imanaka, T. (2005) Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes. Genome Res. 15(3): 352-63. Epub 2005 Feb 14.. PubMed ID: 15710748
372.  (INT) Futterer, O.; Angelov, A.; Liesegang, H.; Gottschalk, G.; Schleper, C.; Schepers, B.; Dock, C.; Antranikian, G.; Liebl, W. (2004) Genome sequence of Picrophilus torridus and its implications for life around pH 0. Proc Natl Acad Sci U S A. 101(24): 9091-6. Epub 2004 Jun 7.. PubMed ID: 15184674
373.  (APP) (PUR) Gaitatzis, N.; Kunze, B.; Muller, R. (2001) In vitro reconstitution of the myxochelin biosynthetic machinery of Stigmatella aurantiaca Sg a15: Biochemical characterization of a reductive release mechanism from nonribosomal peptide synthetases. Proc Natl Acad Sci U S A 98(20): 11136-41. PubMed ID: 11562468
374.  (APP) (PUR) Gan-Erdene, T.; Nagamalleswari, K.; Yin, L.; Wu, K.; Pan, Z. Q.; Wilkinson, K. D. (2003) Identification and characterization of DEN1, a deneddylase of the ULP family. J Biol Chem 278(31): 28892-900. PubMed ID: 12759362
375.  (APP) (PUR) Gan, X.; Ma, Z.; Deng, N.; Wang, J.; Ding, J.; Li, L. (2004) Involvement of the C-terminal proline-rich motif of G protein-coupled receptor kinases in recognition of activated rhodopsin. J Biol Chem 279(48): 49741-6. PubMed ID: 15375171
376.  (INT) Ganesh, N.; Muniyappa, K. (2003) Characterization of DNA strand transfer promoted by Mycobacterium smegmatis RecA reveals functional diversity with Mycobacterium tuberculosis RecA. Biochemistry 42(23): 7216-25. PubMed ID: 12795618
377.  (APP) (PUR) Gangopadhyay, J. P.; Jiang, S. Q.; van Berkel, P.; Paulus, H. (2003) In vitro splicing of erythropoietin by the Mycobacterium tuberculosis RecA intein without substituting amino acids at the splice junctions. Biochim Biophys Acta 1619(2): 193-200. PubMed ID: 12527116
378.  (APP) (CPS) Gangopadhyay, J. P.; Jiang, S. Q.; Paulus, H. (2003B) An in vitro screening system for protein splicing inhibitors based on green fluorescent protein as an indicator. Anal Chem 75(10): 2456-62. PubMed ID: 12918990
379.  (APP) (EPL) (TAG) Gao, W.; Liu, W.; Christensen, T.; Zalutsky, M. R.; Chilkoti, A. (2010) In situ growth of a PEG-like polymer from the C terminus of an intein fusion protein improves pharmacokinetics and tumor accumulation. Proc Natl Acad Sci U S A 107(38): 16432-7. PubMed ID: 20810920
380.  (APP) (TS) Garbe, D.; Thiel, I. V.; Mootz, H. D. (2010) Protein trans-splicing on an M13 bacteriophage: towards directed evolution of a semisynthetic split intein by phage display. J Pept Sci 16(10): 575-81. PubMed ID: 20862725
381.  (APP) (EPL) (TAG) Garner, D. K.; Vaughan, M. D.; Hwang, H. J.; Savelieff, M. G.; Berry, S. M.; Honek, J. F.; Lu, Y. (2006) Reduction Potential Tuning of the Blue Copper Center in Pseudomonas aeruginosa Azurin by the Axial Methionine as Probed by Unnatural Amino Acids. J Am Chem Soc 128(49): 15608-15617. PubMed ID: 17147368
382.  (INT) Garnier, T.; Eiglmeier, K.; Camus, J. C.; Medina, N.; Mansoor, H.; Pryor, M.; Duthoy, S.; Grondin, S.; Lacroix, C.; Monsempe, C.; Simon, S.; Harris, B.; Atkin, R.; Doggett, J.; Mayes, R.; Keating, L.; Wheeler, P. R.; Parkhill, J.; Barrell, B. G.; Cole, S. T.; Gordon, S. V.; Hewinson, R. G. (2003) The complete genome sequence of Mycobacterium bovis. Proc Natl Acad Sci U S A 100(13): 7877-82. PubMed ID: 12788972
383.  (APP) (PUR) Ge, X.; Yang, D. S.; Trabbic-Carlson, K.; Kim, B.; Chilkoti, A.; Filipe, C. D. (2005) Self-cleavable stimulus responsive tags for protein purification without chromatography. J Am Chem Soc. 127(32): 11228-9.. PubMed ID: 16089436
384.  (REL) Geiger, T.; Clarke, S. (1987) Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides. Succinimide-linked reactions that contribute to protein degradation. J Biol Chem 262(2): 785-94. PubMed ID: 3805008
385.  (INT) (REL) Genschik, P.; Billy, E.; Swianiewicz, M.; Filipowicz, W. (1997) The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea. Embo J. 16(10): 2955-67.. PubMed ID: 9184239
386.  (INT) (REL) Genschik, P.; Drabikowski, K.; Filipowicz, W. (1998) Characterization of the Escherichia coli RNA 3'-terminal phosphate cyclase and its sigma54-regulated operon. J Biol Chem. 273(39): 25516-26.. PubMed ID: 9738023
387.  (APP) (PUR) Georgiou, G.; Jeong, K. J. (2005) Proteins from PHB granules. Protein Sci 14(6): 1385-6. PubMed ID: 15929993
388.  (APP) (PUR) Gerasimenko, I.; Sheludko, Y.; Ma, X.; Stockigt, J. (2002) Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur J Biochem 269(8): 2204-13. PubMed ID: 11985599
389.  (APP) (PUR) Gerth, M. L.; Patrick, W. M.; Lutz, S. (2004) A second-generation system for unbiased reading frame selection. Protein Eng Des Sel 17(7): 595-602. PubMed ID: 15331775
390.  (INT) Ghim, S. Y.; Choi, S. K.; Shin, B. S.; Park, S. H. (1998) An 8 kb nucleotide sequence at the 3' flanking region of the sspC gene (184 degrees) on the Bacillus subtilis 168 chromosome containing an intein and an intron. DNA Res 5(2): 121-6. PubMed ID: 9679200
391.  (CPS) (INT) (NEB) (TS) Ghosh, I.; Sun, L.; Xu, M. Q. (2001) Zinc inhibition of protein trans-splicing and identification of regions essential for splicing and association of a split intein*. J Biol Chem 276(26): 24051-8. PubMed ID: 11331276
392.  (NEB) (PUR) (TAG) Ghosh, I.; Sun, L.; Evans, T. C., Jr.; Xu, M. Q. (2004) An improved method for utilization of peptide substrates for antibody characterization and enzymatic assays. J Immunol Methods 293(1-2): 85-95. PubMed ID: 15541279
393.  (REL) Gieselman, M. D.; Xie, L.; van Der Donk, W. A. (2001) Synthesis of a selenocysteine-containing peptide by native chemical ligation. Org Lett. 3(9): 1331-4.. PubMed ID: 11348227
394.  (APP) (EPL) (TAG) Gieselman, M. D.; Zhu, Y.; Zhou, H.; Galonic, D.; Van der Donk, W. A. (2002) Selenocysteine derivatives for chemoselective ligations. Chembiochem 3(8): 709-716.
395.  (APP) (PUR) Gilbert, M.; Stayton, P. S. (1999) Expression and characterization of human salivary statherin from Escherichia coli using two different fusion constructs. Protein Expr Purif 16(2): 243-50. PubMed ID: 10419821
396.  (APP) (PUR) Gillies, A. R.; Hsii, J. F.; Oak, S.; Wood, D. W. (2008) Rapid cloning and purification of proteins: gateway vectors for protein purification by self-cleaving tags. Biotechnol Bioeng 101(2): 229-40. PubMed ID: 18727029
397.  (APP) (CPS) (REV) Gillies, A. R.; Skretas, G.; Wood, D. W. (2008B) Engineered systems for detection and discovery of nuclear hormone-like compounds. Biotechnol Prog 24(1): 8-16. PubMed ID: 18081307
398.  (APP) (PUR) Gillies, A. R.; Mahmoud, R. B.; Wood, D. W. (2009) PHB-intein-mediated protein purification strategy. Methods Mol Biol 498: 173-83. PubMed ID: 18988026
399.  (APP) (CPS) (TS) Gils, M.; Marillonnet, S.; Werner, S.; Grutzner, R.; Giritch, A.; Engler, C.; Schachschneider, R.; Klimyuk, V.; Gleba, Y. (2008) A novel hybrid seed system for plants. Plant Biotechnol J 6(3): 226-35. PubMed ID: 18086236
400.  (EN) (INT) Gimble, F. S.; Thorner, J. (1992) Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae. Nature 357(6376): 301-6. PubMed ID: 1534148
401.  (EN) (INT) Gimble, F. S.; Thorner, J. (1993) Purification and characterization of VDE, a site-specific endonuclease from the yeast Saccharomyces cerevisiae. J Biol Chem 268(29): 21844-53. PubMed ID: 8408039
402.  (EN) (INT) Gimble, F. S.; Stephens, B. W. (1995) Substitutions in conserved dodecapeptide motifs that uncouple the DNA binding and DNA cleavage activities of PI-SceI endonuclease. J Biol Chem 270(11): 5849-56. PubMed ID: 7890714
403.  (EN) (INT) Gimble, F. S.; Wang, J. (1996) Substrate recognition and induced DNA distortion by the PI-SceI endonuclease, an enzyme generated by protein splicing. J Mol Biol 263(2): 163-80. PubMed ID: 8913299
404.  (APP) (REV) Gimble, F. S. (1998) Putting protein splicing to work. Chem Biol 5(10): R251-6. PubMed ID: 9818144
405.  (EN) (INT) Gimble, F. S.; Duan, X.; Hu, D.; Quiocho, F. A. (1998B) Identification of Lys-403 in the PI-SceI homing endonuclease as part of a symmetric catalytic center. J Biol Chem 273(46): 30524-9. PubMed ID: 9804821
406.  (EN) (INT) Gimble, F. S. (2000) Invasion of a multitude of genetic niches by mobile endonuclease genes. FEMS Microbiol Lett 185(2): 99-107. PubMed ID: 10754232
407.  (EN) (INT) Gimble, F. S. (2001) Degeneration of a homing endonuclease and its target sequence in a wild yeast strain. Nucleic Acids Res 29(20): 4215-23. PubMed ID: 11600710
408.  (EN) (INT) Gimble, F. S.; Moure, C. M.; Posey, K. L. (2003) Assessing the plasticity of DNA target site recognition of the PI-SceI homing endonuclease using a bacterial two-hybrid selection system. J Mol Biol 334(5): 993-1008. PubMed ID: 14643662
409.  (EN) Gimble, F. S. (2006) Broken symmetry in homing endonucleases. Structure 14(5): 804-6. PubMed ID: 16698541
410.  (INT) Giovannoni, S. J.; Tripp, H. J.; Givan, S.; Podar, M.; Vergin, K. L.; Baptista, D.; Bibbs, L.; Eads, J.; Richardson, T. H.; Noordewier, M.; Rappe, M. S.; Short, J. M.; Carrington, J. C.; Mathur, E. J. (2005) Genome streamlining in a cosmopolitan oceanic bacterium. Science 309(5738): 1242-5. PubMed ID: 16109880
411.  (APP) (INT) (NEB) (REV) Giriat, I.; Muir, T. W.; Perler, F. B. (2001) Protein splicing and its applications. Genet Eng (N Y) 23: 171-99. PubMed ID: 11570103
412.  (APP) (EPL) (TS) Giriat, I.; Muir, T. W. (2003) Protein semi-synthesis in living cells. J Am Chem Soc 125(24): 7180-1. PubMed ID: 12797783
413.  (APP) (TAG) Girish, A.; Sun, H.; Yeo, D. S.; Chen, G. Y.; Chua, T. K.; Yao, S. Q. (2005) Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing. Bioorg Med Chem Lett 15(10): 2447-51. PubMed ID: 15863295
414.  (INT) Gleason, F. K.; Olszewski, N. E. (2002) Isolation of the gene for the B12-dependent ribonucleotide reductase from Anabaena sp. strain PCC 7120 and expression in Escherichia coli. J Bacteriol 184(23): 6544-50. PubMed ID: 12426342
415.  (APP) (REV) (TS) Gleba, Y.; Marillonnet, S.; Klimyuk, V. (2004) Design of safe and biologically contained transgenic plants: Tools and technologies for controlled transgene flow and expression. Biotechnology and Genetic Engineering Reviews 21: 325-367.
416.  (REV) Go, M. (2001) [Genome organization, function and evolution analyzed on the basis of the three-dimensional structure of proteins]. Tanpakushitsu Kakusan Koso 46(16 Suppl): 2586-91. PubMed ID: 11802434
417.  (EN) (INT) (REL) Goddard, M. R.; Burt, A. (1999) Recurrent invasion and extinction of a selfish gene. Proc Natl Acad Sci U S A. 96(24): 13880-5.. PubMed ID: 10570167
418.  (EN) (INT) Goddard, M. R.; Greig, D.; Burt, A. (2001) Outcrossed sex allows a selfish gene to invade yeast populations. Proc Biol Sci. 268(1485): 2537-42.. PubMed ID: 11749707
419.  (INT) (REV) (Structure) Gogarten, J. P.; Senejani, A. G.; Zhaxybayeva, O.; Olendzenski, L.; Hilario, E. (2002) Inteins: structure, function, and evolution. Annu Rev Microbiol 56: 263-87. PubMed ID: 12142479
420.  (INT) (REV) Gogarten, J. P.; Hilario, E. (2006) Inteins, introns, and homing endonucleases: recent revelations about the life cycle of parasitic genetic elements. BMC Evol Biol 6: 94. PubMed ID: 17101053
421.  (APP) (PUR) Gold, B.; Deng, H.; Bryk, R.; Vargas, D.; Eliezer, D.; Roberts, J.; Jiang, X.; Nathan, C. (2008) Identification of a copper-binding metallothionein in pathogenic mycobacteria. Nat Chem Biol 4(10): 609-16. PubMed ID: 18724363
422.  (APP) (PUR) Goodin, J. L.; Raab, R. W.; McKown, R. L.; Coffman, G. L.; Powell, B. S.; Enama, J. T.; Ligon, J. A.; Andrews, G. P. (2005) Yersinia pestis outer membrane type III secretion protein YscC: expression, purification, characterization, and induction of specific antiserum. Protein Expr Purif 40(1): 152-63. PubMed ID: 15721783
423.  (REL) Goodrich, L. V.; Johnson, R. L.; Milenkovic, L.; McMahon, J. A.; Scott, M. P. (1996) Conservation of the hedgehog/patched signaling pathway from flies to mice: induction of a mouse patched gene by Hedgehog. Genes Dev. 10(3): 301-12.. PubMed ID: 8595881
424.  (INT) Goodwin, T. J.; Butler, M. I.; Poulter, R. T. (2006) Multiple, non-allelic, intein-coding sequences in eukaryotic RNA polymerase genes. BMC Biol 4(1): 38. PubMed ID: 17069655
425.  (APP) (EPL) (PUR) (REL) Goody, R. S.; Durek, T.; Waldmann, H.; Brunsveld, L.; Alexandrov, K. (2005) Application of protein semisynthesis for the construction of functionalized posttranslationally modified rab GTPases. Methods Enzymol. 403: 29-42.. PubMed ID: 16473575
426.  (INT) Gorbalenya, A. E. (1998) Non-canonical inteins. Nucleic Acids Res 26(7): 1741-8. PubMed ID: 9512547
427.  (APP) (EPL) Gottlieb, D.; Grunwald, C.; Nowak, C.; Kuhlmann, J.; Waldmann, H. (2006) Intein-mediated in vitro synthesis of lipidated Ras proteins. Chem Commun (Camb) 3: 260-2. PubMed ID: 16391727
428.  (APP) (EPL) (TAG) Grant, J. E.; Guo, L. W.; Vestling, M. M.; Martemyanov, K. A.; Arshavsky, V. Y.; Ruoho, A. E. (2006) The N Terminus of GTP{gamma}S-activated Transducin {alpha}-Subunit Interacts with the C Terminus of the cGMP Phosphodiesterase {gamma}-Subunit. J Biol Chem 281(10): 6194-202. PubMed ID: 16407279
429.  (REL) (REV) Gregan, J. (2005) Conditional alleles, inteins and degrons. Nat Biotechnol 23(1): 18. PubMed ID: 15637609
430.  (INT) Griffiths, K.; Nayak, S.; Park, K.; Mandelman, D.; Modrell, B.; Lee, J.; Ng, B.; Gibbs, M. D.; Bergquist, P. L. (2007) New high fidelity polymerases from Thermococcus species. Protein Expr Purif 52(1): 19-30. PubMed ID: 16982200
431.  (EN) (INT) Grindl, W.; Wende, W.; Pingoud, V.; Pingoud, A. (1998) The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding. Nucleic Acids Res 26(8): 1857-62. PubMed ID: 9518476
432.  (APP) (EPL) (TAG) Grogan, M. J.; Kaizuka, Y.; Conrad, R. M.; Groves, J. T.; Bertozzi, C. R. (2005) Synthesis of lipidated green fluorescent protein and its incorporation in supported lipid bilayers. J Am Chem Soc 127(41): 14383-7. PubMed ID: 16218633
433.  (INT) (REV) Gross, V.M. (1997, February 2) Inteine - selbst-spleissende proteine. Spektrum der Wissenschaft 16-22.
434.  (APP) (PUR) Gross, F.; Gottschalk, D.; Muller, R. (2005) Posttranslational modification of myxobacterial carrier protein domains in Pseudomonas sp. by an intrinsic phosphopantetheinyl transferase. Appl Microbiol Biotechnol 68(1): 66-74. PubMed ID: 15635461
435.  (EN) Gruen, M.; Chang, K.; Serbanescu, I.; Liu, D. R. (2002) An in vivo selection system for homing endonuclease activity. Nucleic Acids Res 30(7): e29. PubMed ID: 11917035
436.  (APP) (PUR) Grzybowska, B.; Szweda, P.; Synowiecki, J. (2004) Cloning of the thermostable alpha-amylase gene from Pyrococcus woesei in Escherichia coli: isolation and some properties of the enzyme. Mol Biotechnol 26(2): 101-10. PubMed ID: 14764935
437.  (NEB) (REL) Guan, C.; Cui, T.; Rao, V.; Liao, W.; Benner, J.; Lin, C. L.; Comb, D. (1996) Activation of glycosylasparaginase. Formation of active N-terminal threonine by intramolecular autoproteolysis. J Biol Chem. 271(3): 1732-7.. PubMed ID: 8576176
438.  (NEB) (REL) Guan, C.; Liu, Y.; Shao, Y.; Cui, T.; Liao, W.; Ewel, A.; Whitaker, R.; Paulus, H. (1998) Characterization and functional analysis of the cis-autoproteolysis active center of glycosylasparaginase. J Biol Chem. 273(16): 9695-702.. PubMed ID: 9545304
439.  (APP) (NEB) (PUR) (TS) Guan, C.; Kumar, S. (2005) A single catalytic domain of the junction-resolving enzyme T7 endonuclease I is a non-specific nicking endonuclease. Nucleic Acids Res 33(19): 6225-34. PubMed ID: 16264086
440.  (INT) Gueguen, Y.; Rolland, J. L.; Lecompte, O.; Azam, P.; Le Romancer, G.; Flament, D.; Raffin, J. P.; Dietrich, J. (2001) Characterization of two DNA polymerases from the hyperthermophilic euryarchaeon Pyrococcus abyssi. Eur J Biochem 268(22): 5961-9. PubMed ID: 11722585
441.  (EN) (INT) Guhan, N.; Muniyappa, K. (2002) Mycobacterium tuberculosis RecA intein possesses a novel ATP-dependent site-specific double-stranded DNA endonuclease activity. J Biol Chem 277(18): 16257-64. PubMed ID: 11850426
442.  (EN) (INT) Guhan, N.; Muniyappa, K. (2002B) The RecA intein of Mycobacterium tuberculosis promotes cleavage of ectopic DNA sites. Implications for the dispersal of inteins in natural populations. J Biol Chem 277(43): 40352-61. PubMed ID: 12167644
443.  (EN) (REV) Guhan, N.; Muniyappa, K. (2003) Structural and functional characteristics of homing endonucleases. Crit Rev Biochem Mol Biol 38(3): 199-248. PubMed ID: 12870715
444.  (EN) (INT) Guhan, N.; Muniyappa, K. (2003B) Mycobacterium tuberculosis RecA intein, a LAGLIDADG homing endonuclease, displays Mn(2+) and DNA-dependent ATPase activity. Nucleic Acids Res 31(14): 4184-91. PubMed ID: 12853636
445.  (CYCL) (REL) Gunasekera, S.; Daly, N. L.; Anderson, M. A.; Craik, D. J. (2006) Chemical synthesis and biosynthesis of the cyclotide family of circular proteins. IUBMB Life 58(9): 515-24. PubMed ID: 17002979
446.  (APP) (NEB) (PUR) Guo, C.; Li, Z.; Shi, Y.; Xu, M.; Wise, J. G.; Trommer, W. E.; Yuan, J. (2004) Intein-mediated fusion expression, high efficient refolding, and one-step purification of gelonin toxin. Protein Expr Purif 37(2): 361-7. PubMed ID: 15358358
447.  (APP) (PUR) Guo, L. W.; Grant, J. E.; Hajipour, A. R.; Muradov, H.; Arbabian, M.; Artemyev, N. O.; Ruoho, A. E. (2005) Asymmetric interaction between rod cyclic GMP phosphodiesterase gamma subunits and alphabeta subunits. J Biol Chem 280(13): 12585-92. PubMed ID: 15668239
448.  (APP) (EPL) (PUR) Hackenberger, C. P.; Chen, M. M.; Imperiali, B. (2006) Expression of N-terminal Cys-protein fragments using an intein refolding strategy. Bioorg Med Chem 14(14): 5043-8. PubMed ID: 16554163
449.  (APP) (EPL) (TAG) Hackenberger, C. P. (2007) A synthetic kiss of death: expressed protein ligation of a ubiquitin-peptide conjugate. Chembiochem 8(11): 1221-3. PubMed ID: 17551918
450.  (APP) (EPL) (REV) Hackenberger, C. P.; Schwarzer, D. (2008) Chemoselective ligation and modification strategies for peptides and proteins. Angew Chem Int Ed Engl 47(52): 10030-74. PubMed ID: 19072788
451.  (EPL) (REL) Hackeng, T. M.; Griffin, J. H.; Dawson, P. E. (1999) Protein synthesis by native chemical ligation: expanded scope by using straightforward methodology. Proc Natl Acad Sci U S A 96(18): 10068-73. PubMed ID: 10468563
452.  (APP) (EPL) Hahn, M. E.; Muir, T. W. (2004) Photocontrol of Smad2, a multiphosphorylated cell-signaling protein, through caging of activating phosphoserines. Angew Chem Int Ed Engl. 43(43): 5800-3.. PubMed ID: 15523718
453.  (APP) (EPL) (REV) Hahn, M. E.; Muir, T. W. (2005) Manipulating proteins with chemistry: a cross-section of chemical biology. Trends Biochem Sci. 30(1): 26-34.. PubMed ID: 15653323
454.  (APP) (EPL) (TAG) Hahn, M. E.; Pellois, J. P.; Vila-Perello, M.; Muir, T. W. (2007) Tunable photoactivation of a post-translationally modified signaling protein and its unmodified counterpart in live cells. Chembiochem 8(17): 2100-5. PubMed ID: 17907120
455.  (APP) (EPL) Hak, J. K.; Hee, J. S.; Hyun, W. K.; Kang, S. H.; Young, T. K. (2007) Expressed protein ligation of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase: An application to a protein expressed as an inclusion body. Bulletin of the Korean Chemical Society 28(12): 2303-2309.
456.  (REL) (Structure) Hall, T. M.; Porter, J. A.; Young, K. E.; Koonin, E. V.; Beachy, P. A.; Leahy, D. J. (1997) Crystal structure of a Hedgehog autoprocessing domain: homology between Hedgehog and self-splicing proteins. Cell 91(1): 85-97. PubMed ID: 9335337
457.  (APP) (PUR) Hall, M. C.; Kunkel, T. A. (2001) Purification of eukaryotic MutL homologs from Saccharomyces cerevisiae using self-affinity technology. Protein Expr Purif 21(2): 333-42. PubMed ID: 11237696
458.  (REL) Hanada, K.; Yewdell, J. W.; Yang, J. C. (2004) Immune recognition of a human renal cancer antigen through post-translational protein splicing. Nature 427(6971): 252-6. PubMed ID: 14724640
459.  (REL) Hanada, K.; Yang, J. C. (2005) Novel biochemistry: post-translational protein splicing and other lessons from the school of antigen processing. J Mol Med 83(6): 420-8. PubMed ID: 15759099
460.  (APP) (EPL) (REV) Hang, H. C.; Bertozzi, C. R. (2001) Chemoselective approaches to glycoprotein assembly. Acc Chem Res. 34(9): 727-36.. PubMed ID: 11560472
461.  (APP) (PUR) Hanzawa, H.; Inomata, K.; Kinoshita, H.; Kakiuchi, T.; Jayasundera, K. P.; Sawamoto, D.; Ohta, A.; Uchida, K.; Wada, K.; Furuya, M. (2001) In vitro assembly of phytochrome B apoprotein with synthetic analogs of the phytochrome chromophore. Proc Natl Acad Sci U S A 98(6): 3612-7. PubMed ID: 11248126
462.  (APP) (REV) Hao, D. M.; Qian, K. X.; Shen, G. F. (2004) [Protein splicing and its application in protein engineering.]. Yi Chuan 26(2): 249-52. PubMed ID: 15639997
463.  (APP) (PUR) Hao, C. J.; Xu, C. G.; Wang, W.; Chai, B. F.; Liang, A. H. (2005) Expression of an insect excitatory toxin, BmK IT, from the scorpion, buthus martensii Karsch, and its biological activity. Biotechnol Lett 27(23-24): 1929-34. PubMed ID: 16328992
464.  (APP) (PUR) (REV) Hao, X.; Cheng, Y.; Li-E, C. (2009) Current strategies for polypeptide fusion tags removal. Progress in Biochemistry and Biophysics 36(10): 1364-1669.
465.  (EN) (INT) (Structure) Hashimoto, H.; Takahashi, H.; Nishioka, M.; Fujiwara, S.; Takagi, M.; Imanaka, T.; Inoue, T.; Kai, Y. (2000) Crystallographic study of intein homing endonuclease II encoded in the archaeal DNA polymerase gene. Acta Crystallogr D Biol Crystallogr 56(( Pt 9)): 1185-6. PubMed ID: 10957641
466.  (INT) (Structure) Hashimoto, H.; Nishioka, M.; Fujiwara, S.; Takagi, M.; Imanaka, T.; Inoue, T.; Kai, Y. (2001) Crystal structure of DNA polymerase from hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1. J Mol Biol 306(3): 469-77. PubMed ID: 11178906
467.  (APP) Hassiepen, U.; Eidhoff, U.; Meder, G.; Bulber, J. F.; Hein, A.; Bodendorf, U.; Lorthiois, E.; Martoglio, B. (2007) A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine110-glycine as substrate. Anal Biochem 371(2): 201-7. PubMed ID: 17869210
468.  (INT) Hatfull, G. F.; Pedulla, M. L.; Jacobs-Sera, D.; Cichon, P. M.; Foley, A.; Ford, M. E.; Gonda, R. M.; Houtz, J. M.; Hryckowian, A. J.; Kelchner, V. A.; Namburi, S.; Pajcini, K. V.; Popovich, M. G.; Schleicher, D. T.; Simanek, B. Z.; Smith, A. L.; Zdanowicz, G. M.; Kumar, V.; Peebles, C. L.; Jacobs, W. R., Jr.; Lawrence, J. G.; Hendrix, R. W. (2006) Exploring the mycobacteriophage metaproteome: phage genomics as an educational platform. PLoS Genet 2(6): e92. PubMed ID: 16789831
469.  (EN) Haugen, P.; Wikmark, O. G.; Vader, A.; Coucheron, D. H.; Sjottem, E.; Johansen, S. D. (2005) The recent transfer of a homing endonuclease gene. Nucleic Acids Res 33(8): 2734-41. PubMed ID: 15891115
470.  (APP) (EPL) (PUR) Hauser, P. S.; Ryan, R. O. (2007) Expressed protein ligation using an N-terminal cysteine containing fragment generated in vivo from a pelB fusion protein. Protein Expr Purif 54(2): 227-33. PubMed ID: 17493830
471.  (APP) (EPL) (NMR) (Structure) Hauser, P. S.; Raussens, V.; Yamamoto, T.; Abdullahi, G. E.; Weers, P. M.; Sykes, B. D.; Ryan, R. O. (2009) Semisynthesis and segmental isotope labeling of the apoE3 N-terminal domain using expressed protein ligation. J Lipid Res 50(8): 1548-55. PubMed ID: 19098282
472.  (EN) (INT) He, Z.; Crist, M.; Yen, H.; Duan, X.; Quiocho, F. A.; Gimble, F. S. (1998) Amino acid residues in both the protein splicing and endonuclease domains of the PI-SceI intein mediate DNA binding. J Biol Chem 273(8): 4607-15. PubMed ID: 9468518
473.  (EN) (Structure) Heath, P. J.; Stephens, K. M.; Monnat, R. J., Jr.; Stoddard, B. L. (1997) The structure of I-Crel, a group I intron-encoded homing endonuclease. Nat Struct Biol 4(6): 468-76. PubMed ID: 9187655
474.  (INT) (NMR) (Structure) Heinamaki, K.; Oeemig, J. S.; Djupsjobacka, J.; Iwai, H. (2009) NMR resonance assignment of DnaE intein from Nostoc punctiforme. Biomol NMR Assign 3(1): 41-3. PubMed ID: 19636943
475.  (APP) (PUR) Hemelaar, J.; Lelyveld, V. S.; Kessler, B. M.; Ploegh, H. L. (2003) A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L. J Biol Chem 278(51): 51841-50. PubMed ID: 14530254
476.  (APP) (EPL) (TAG) Hemelaar, J.; Borodovsky, A.; Kessler, B. M.; Reverter, D.; Cook, J.; Kolli, N.; Gan-Erdene, T.; Wilkinson, K. D.; Gill, G.; Lima, C. D.; Ploegh, H. L.; Ovaa, H. (2004) Specific and covalent targeting of conjugating and deconjugating enzymes of ubiquitin-like proteins. Mol Cell Biol 24(1): 84-95. PubMed ID: 14673145
477.  (APP) (EPL) (REL) (REV) Hemelaar, J.; Galardy, P. J.; Borodovsky, A.; Kessler, B. M.; Ploegh, H. L.; Ovaa, H. (2004) Chemistry-Based Functional Proteomics: Mechanism-Based Activity-Profiling Tools for Ubiquitin and Ubiquitin-like Specific Proteases. Journal of Proteome Research 3(2): 268-276.
478.  (INT) Hendrickson, E. L.; Kaul, R.; Zhou, Y.; Bovee, D.; Chapman, P.; Chung, J.; Conway de Macario, E.; Dodsworth, J. A.; Gillett, W.; Graham, D. E.; Hackett, M.; Haydock, A. K.; Kang, A.; Land, M. L.; Levy, R.; Lie, T. J.; Major, T. A.; Moore, B. C.; Porat, I.; Palmeiri, A.; Rouse, G.; Saenphimmachak, C.; Soll, D.; Van Dien, S.; Wang, T.; Whitman, W. B.; Xia, Q.; Zhang, Y.; Larimer, F. W.; Olson, M. V.; Leigh, J. A. (2004) Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis. J Bacteriol 186(20): 6956-69. PubMed ID: 15466049
479.  (REL) (REV) Hendrix, R. W. (1991) Protein carpentry. Curr Biol. 1(2): 71-3.. PubMed ID: 15336168
480.  (INT) Henne, A.; Bruggemann, H.; Raasch, C.; Wiezer, A.; Hartsch, T.; Liesegang, H.; Johann, A.; Lienard, T.; Gohl, O.; Martinez-Arias, R.; Jacobi, C.; Starkuviene, V.; Schlenczeck, S.; Dencker, S.; Huber, R.; Klenk, H. P.; Kramer, W.; Merkl, R.; Gottschalk, G.; Fritz, H. J. (2004) The genome sequence of the extreme thermophile Thermus thermophilus. Nat Biotechnol. 22(5): 547-53. Epub 2004 Apr 4.. PubMed ID: 15064768
481.  (INT) Henneke, G.; Gueguen, Y.; Flament, D.; Azam, P.; Querellou, J.; Dietrich, J.; Hubscher, U.; Raffin, J. P. (2002) Replication factor C from the hyperthermophilic archaeon Pyrococcus abyssi does not need ATP hydrolysis for clamp-loading and contains a functionally conserved RFC PCNA-binding domain. J Mol Biol 323(5): 795-810. PubMed ID: 12417194
482.  (APP) (PUR) Herrmann, R.; Heck, M.; Henklein, P.; Kleuss, C.; Hofmann, K. P.; Ernst, O. P. (2004) Sequence of interactions in receptor-G protein coupling. J Biol Chem 279(23): 24283-90. PubMed ID: 15007073
483.  (INT) Hertveldt, K.; Lavigne, R.; Pleteneva, E.; Sernova, N.; Kurochkina, L.; Korchevskii, R.; Robben, J.; Mesyanzhinov, V.; Krylov, V. N.; Volckaert, G. (2005) Genome comparison of Pseudomonas aeruginosa large phages. J Mol Biol 354(3): 536-45. PubMed ID: 16256135
484.  (INT) (REV) Hickey, D. A. (1994) Protein introns: optional or essential?. Trends Genet. 10(5): 147-9.. PubMed ID: 8036715
485.  (EN) (INT) Hiraga, K.; Derbyshire, V.; Dansereau, J. T.; Van Roey, P.; Belfort, M. (2005) Minimization and stabilization of the Mycobacterium tuberculosis recA intein. J Mol Biol 354(4): 916-26. PubMed ID: 16288917
486.  (INT) Hiraga, K.; Soga, I.; Dansereau, J. T.; Pereira, B.; Derbyshire, V.; Du, Z.; Wang, C.; Van Roey, P.; Belfort, G.; Belfort, M. (2009) Selection and structure of hyperactive inteins: peripheral changes relayed to the catalytic center. J Mol Biol 393(5): 1106-17. PubMed ID: 19744499
487.  (INT) Hirata, R.; Ohsumk, Y.; Nakano, A.; Kawasaki, H.; Suzuki, K.; Anraku, Y. (1990) Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. J Biol Chem. 265(12): 6726-33.. PubMed ID: 2139027
488.  (INT) Hirata, R.; Anraku, Y. (1992) Mutations at the putative junction sites of the yeast VMA1 protein, the catalytic subunit of the vacuolar membrane H(+)-ATPase, inhibit its processing by protein splicing. Biochem Biophys Res Commun 188(1): 40-7. PubMed ID: 1417861
489.  (APP) (PUR) Hoang, T. T.; Ma, Y.; Stern, R. J.; McNeil, M. R.; Schweizer, H. P. (1999) Construction and use of low-copy number T7 expression vectors for purification of problem proteins: purification of mycobacterium tuberculosis RmlD and pseudomonas aeruginosa LasI and RhlI proteins, and functional analysis of purified RhlI. Gene 237(2): 361-71. PubMed ID: 10521660
490.  (EN) (INT) (NEB) Hodges, R. A.; Perler, F. B.; Noren, C. J.; Jack, W. E. (1992) Protein splicing removes intervening sequences in an archaea DNA polymerase. Nucleic Acids Res 20(23): 6153-7. PubMed ID: 1475179
491.  (APP) (EPL) (REV) Hodgson, D. R. W.; Sanderson, J. M. (2004) The synthesis of peptides and proteins containing non-natural amino acids. Chemical Society Reviews 33(7): 422-430.
492.  (APP) (EPL) (TAG) Hofmann, R. M.; Cotton, G. J.; Chang, E. J.; Vidal, E.; Veach, D.; Bornmann, W.; Muir, T. W. (2001) Fluorescent monitoring of kinase activity in real time: development of a robust fluorescence-based assay for Abl tyrosine kinase activity. Bioorg Med Chem Lett. 11(24): 3091-4.. PubMed ID: 11720849
493.  (APP) (EPL) (REV) Hofmann, R. M.; Muir, T. W. (2002) Recent advances in the application of expressed protein ligation to protein engineering. Curr Opin Biotechnol 13(4): 297-303. PubMed ID: 12323349
494.  (APP) (PUR) (Structure) (TS) Hofmann, A.; Iwai, H.; Hess, S.; Pluckthun, A.; Wlodawer, A. (2002B) Structure of cyclized green fluorescent protein. Acta Crystallogr D Biol Crystallogr 58(Pt 9): 1400-6. PubMed ID: 12198295
495.  (REV) (TS) Holden, M.; Sebaihia, M.; Cerdeno-Tarraga, A.; Parkhill, J. (2002) Split personalities. Trends Microbiol. 10(3): 115.. PubMed ID: 11864813
496.  (APP) (EPL) (PUR) (TAG) Holder, J. W.; Craig, N. L. (2010) Architecture of the Tn7 posttransposition complex: an elaborate nucleoprotein structure. J Mol Biol 401(2): 167-81. PubMed ID: 20538004
497.  (APP) (REV) Holford, M.; Muir, T. W. (1998) Adding 'splice' to protein engineering. Structure 6(8): 951-6. PubMed ID: 9739093
498.  (APP) (EPL) (TAG) Holland-Nell, K.; Beck-Sickinger, A. G. (2007) Specifically Immobilised Aldo/Keto Reductase AKR1A1 Shows a Dramatic Increase in Activity Relative to the Randomly Immobilised Enzyme. Chembiochem 8(9): 1071-1076. PubMed ID: 17508367
499.  (APP) (EPL) (TAG) Hondal, R. J.; Nilsson, B. L.; Raines, R. T. (2001) Selenocysteine in native chemical ligation and expressed protein ligation. J Am Chem Soc 123(21): 5140-1. PubMed ID: 11457362
500.  (APP) (EPL) (TAG) Hondal, R. J. (2005) Incorporation of selenocysteine into proteins using peptide ligation. Protein Pept Lett 12(8): 757-64. PubMed ID: 16305545
501.  (APP) (EPL) (REV) Hondal, R. J. (2009) Using chemical approaches to study selenoproteins-Focus on thioredoxin reductases. Biochim Biophys Acta 1790(11): 1501-12. PubMed ID: 19406205
502.  (APP) (PUR) Hong, S.; Toyama, M.; Maret, W.; Murooka, Y. (2001) High yield expression and single step purification of human thionein/metallothionein. Protein Expr Purif 21(1): 243-50. PubMed ID: 11162412
503.  (APP) (EPL) (TAG) Hong, S. H.; Maret, W. (2003) A fluorescence resonance energy transfer sensor for the beta-domain of metallothionein. Proc Natl Acad Sci U S A. 100(5): 2255-60.. PubMed ID: 12618543
504.  (APP) (PUR) Hong, J.; Wang, Y.; Ye, X.; Zhang, Y. H. (2008) Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage. J Chromatogr A 1194(2): 150-154. PubMed ID: 18468611
505.  (APP) (PUR) Hong, I.; Kim, Y. S.; Choi, S. G. (2010) Simple purification of human antimicrobial peptide dermcidin (MDCD-1L) by intein-mediated expression in E.coli. J Microbiol Biotechnol 20(2): 350-5. PubMed ID: 20208440
506.  (APP) (EPL) (TAG) Hori, Y.; Egashira, Y.; Kamiura, R.; Kikuchi, K. (2010) Noncovalent-interaction-promoted ligation for protein labeling. Chembiochem 11(5): 646-648.
507.  (APP) (CYCL) (TS) Horswill, A. R.; Savinov, S. N.; Benkovic, S. J. (2004) A systematic method for identifying small-molecule modulators of protein-protein interactions. Proc Natl Acad Sci U S A 101(44): 15591-6. PubMed ID: 15498867
508.  (APP) (CYCL) (REV) Horswill, A. R.; Benkovic, S. J. (2005) Cyclic peptides, a chemical genetics tool for biologists. Cell Cycle 4(4): 552-5. PubMed ID: 15876867
509.  (APP) (CYCL) (REV) Horswill, A. R.; Benkovic, S. J. (2006) Identifying small-molecule modulators of protein-protein interactions. Curr Protoc Protein Sci Chapter 19: Unit 19 15. PubMed ID: 18429304
510.  (EN) (INT) Hu, D.; Crist, M.; Duan, X.; Gimble, F. S. (1999) Mapping of a DNA binding region of the PI-sceI homing endonuclease by affinity cleavage and alanine-scanning mutagenesis. Biochemistry 38(39): 12621-8. PubMed ID: 10504231
511.  (EN) (INT) Hu, D.; Crist, M.; Duan, X.; Quiocho, F. A.; Gimble, F. S. (2000) Probing the structure of the PI-SceI-DNA complex by affinity cleavage and affinity photocross-linking. J Biol Chem 275(4): 2705-12. PubMed ID: 10644733
512.  (INT) Huang, C.; Wang, S.; Chen, L.; Lemieux, C.; Otis, C.; Turmel, M.; Liu, X. Q. (1994) The Chlamydomonas chloroplast clpP gene contains translated large insertion sequences and is essential for cell growth. Mol Gen Genet. 244(2): 151-9.. PubMed ID: 8052234
513.  (INT) Huet, G.; Daffe, M.; Saves, I. (2005) Identification of the Mycobacterium tuberculosis SUF machinery as the exclusive mycobacterial system of [Fe-S] cluster assembly: evidence for its implication in the pathogen's survival. J Bacteriol 187(17): 6137-46. PubMed ID: 16109955
514.  (INT) Huet, G.; Castaing, J. P.; Fournier, D.; Daffe, M.; Saves, I. (2006) Protein Splicing of SufB Is Crucial for the Functionality of the Mycobacterium tuberculosis SUF Machinery. J Bacteriol. 188(9): 3412-4. PubMed ID: 16621837
515.  (APP) (PUR) Humphries, H. E.; Christodoulides, M.; Heckels, J. E. (2002) Expression of the class 1 outer-membrane protein of Neisseria meningitidis in Escherichia coli and purification using a self-cleavable affinity tag. Protein Expr Purif 26(2): 243-8. PubMed ID: 12406678
516.  (APP) (PUR) Humphries, H. E.; Williams, J. N.; Christodoulides, M.; Heckels, J. E. (2004) Recombinant meningococcal PorA protein, expressed using a vector system with potential for human vaccination, induces a bactericidal immune response. Vaccine 22(11-12): 1564-9. PubMed ID: 15063582
517.  (APP) (PUR) (REV) Hunt, I. (2005) From gene to protein: a review of new and enabling technologies for multi-parallel protein expression. Protein Expr Purif 40(1): 1-22. PubMed ID: 15721767
518.  (APP) (REL) Hunter, C. L.; Kochendoerfer, G. G. (2004) Native chemical ligation of hydrophobic [corrected] peptides in lipid bilayer systems. Bioconjug Chem. 15(3): 437-40.. PubMed ID: 15149169
519.  (APP) (EPL) Huse, M.; Holford, M.N.; Kuriyan, J.; Muir, T.W. (2000) Semisynthesis of Hyperphosphorylated Type I Tgf beta Receptor: Addressing the Mechanism of Kinase Activation. J Amer Chem Soc 122(34): 8337-8338.
520.  (APP) (EPL) Huse, M.; Muir, T. W.; Xu, L.; Chen, Y. G.; Kuriyan, J.; Massague, J. (2001) The TGF beta receptor activation process: an inhibitor- to substrate-binding switch. Mol Cell. 8(3): 671-82.. PubMed ID: 11583628
521.  (INT) Iacono, M.; Villa, L.; Fortini, D.; Bordoni, R.; Imperi, F.; Bonnal, R. J.; Sicheritz-Ponten, T.; De Bellis, G.; Visca, P.; Cassone, A.; Carattoli, A. (2008) Whole-genome pyrosequencing of an epidemic multidrug-resistant Acinetobacter baumannii strain belonging to the European clone II group. Antimicrob Agents Chemother 52(7): 2616-25. PubMed ID: 18411315
522.  (APP) (EPL) (PUR) (REL) Iakovenko, A.; Rostkova, E.; Merzlyak, E.; Hillebrand, A. M.; Thoma, N. H.; Goody, R. S.; Alexandrov, K. (2000) Semi-synthetic Rab proteins as tools for studying intermolecular interactions. FEBS Lett. 468(2-3): 155-8.. PubMed ID: 10692577
523.  (EN) (INT) (Structure) Ichiyanagi, K.; Ishino, Y.; Ariyoshi, M.; Komori, K.; Morikawa, K. (2000) Crystal structure of an archaeal intein-encoded homing endonuclease PI-PfuI. J Mol Biol 300(4): 889-901. PubMed ID: 10891276
524.  (INT) Ikeda, H.; Ishikawa, J.; Hanamoto, A.; Shinose, M.; Kikuchi, H.; Shiba, T.; Sakaki, Y.; Hattori, M.; Omura, S. (2003) Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis. Nat Biotechnol. 21(5): 526-31. Epub 2003 Apr 14.. PubMed ID: 12692562
525.  (APP) (PUR) (REL) (Structure) Ikegami, T.; Okada, T.; Hashimoto, M.; Seino, S.; Watanabe, T.; Shirakawa, M. (2000) Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1. J Biol Chem 275(18): 13654-61. PubMed ID: 10788483
526.  (APP) (REL) (TAG) Inard, C.; Fourcade, E.; Baron, R.; Tovar, D.; Chaisemartin, L.; Blonski, C.; Faye, J. C. (2006) Syntheses of functionalized biotin N-1' derivatives: new tools for the control of gene expression with small molecules. Bioconjug Chem 17(4): 1030-5. PubMed ID: 16848412
527.  (APP) (PUR) Ingham, A. B.; Sproat, K. W.; Tizard, M. L.; Moore, R. J. (2005) A versatile system for the expression of nonmodified bacteriocins in Escherichia coli. J Appl Microbiol 98(3): 676-83. PubMed ID: 15715871
528.  (APP) (PUR) Inoue, K.; Demel, R.; de Kruijff, B.; Keegstra, K. (2001) The N-terminal portion of the preToc75 transit peptide interacts with membrane lipids and inhibits binding and import of precursor proteins into isolated chloroplasts. Eur J Biochem 268(14): 4036-43. PubMed ID: 11453998
529.  (INT) Ishikawa, J.; Yamashita, A.; Mikami, Y.; Hoshino, Y.; Kurita, H.; Hotta, K.; Shiba, T.; Hattori, M. (2004) The complete genomic sequence of Nocardia farcinica IFM 10152. Proc Natl Acad Sci U S A. 101(41): 14925-30. Epub 2004 Oct 4.. PubMed ID: 15466710
530.  (INT) Ivars-Martinez, E.; Martin-Cuadrado, A. B.; D'Auria, G.; Mira, A.; Ferriera, S.; Johnson, J.; Friedman, R.; Rodriguez-Valera, F. (2008) Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter. ISME J 2(12): 1194-212. PubMed ID: 18670397
531.  (REL) (REV) Iwai, K.; Ando, T. (1967) N-O acyl shifts. Meth. Enzymol. 11: 262-282.
532.  (APP) (CYCL) Iwai, H.; Pluckthun, A. (1999) Circular beta-lactamase: stability enhancement by cyclizing the backbone. FEBS Lett 459(2): 166-72. PubMed ID: 10518012
533.  (APP) (CYCL) Iwai, H.; Lingel, A.; Pluckthun, A. (2001) Cyclic green fluorescent protein produced in vivo using an artificially split PI-PfuI intein from Pyrococcus furiosus. J Biol Chem 276(19): 16548-54. PubMed ID: 11278952
534.  (INT) (TS) Iwai, H.; Zuger, S.; Jin, J.; Tam, P. H. (2006) Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme. FEBS Lett 580(7): 1853-1858. PubMed ID: 16516207
535.  (APP) (NMR) (REV) (Structure) Iwai, H.; Zuger, S. (2007) Protein ligation: applications in NMR studies of proteins. Biotechnol Genet Eng Rev 24: 129-45. PubMed ID: 18059630
536.  (INT) Iwai, H. (2010) Hideo Iwai. Interview by Daniela Ruffell. FEBS Lett 584(12): 2494-5. PubMed ID: 20450913
537.  (REL) Jabs, A.; Weiss, M. S.; Hilgenfeld, R. (1999) Non-proline cis peptide bonds in proteins. J Mol Biol. 286(1): 291-304.. PubMed ID: 9931267
538.  (REL) (REV) Jaffe, S. (2004) Humanizing Protein Splicing. The Scientist 18(11): 30-31.
539.  (INT) Jakob, N. J.; Muller, K.; Bahr, U.; Darai, G. (2001) Analysis of the first complete DNA sequence of an invertebrate iridovirus: coding strategy of the genome of Chilo iridescent virus. Virology 286(1): 182-96. PubMed ID: 11448171
540.  (APP) Jantz, D.; Berg, J. M. (2003) Expanding the DNA-recognition repertoire for zinc finger proteins beyond 20 amino acids. J Am Chem Soc. 125(17): 4960-1.. PubMed ID: 12708829
541.  (APP) (CYCL) Jeffries, C. M.; Graham, S. C.; Stokes, P. H.; Collyer, C. A.; Guss, J. M.; Matthews, J. M. (2006) Stabilization of a binary protein complex by intein-mediated cyclization. Protein Sci 15(11): 2612-8. PubMed ID: 17001033
542.  (INT) Jeffries, T. W.; Grigoriev, I. V.; Grimwood, J.; Laplaza, J. M.; Aerts, A.; Salamov, A.; Schmutz, J.; Lindquist, E.; Dehal, P.; Shapiro, H.; Jin, Y. S.; Passoth, V.; Richardson, P. M. (2007) Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis. Nat Biotechnol 25(3): 319-326. PubMed ID: 17334359
543.  (APP) (PUR) Jester, B. C.; Levengood, J. D.; Roy, H.; Ibba, M.; Devine, K. M. (2003) Nonorthologous replacement of lysyl-tRNA synthetase prevents addition of lysine analogues to the genetic code. Proc Natl Acad Sci U S A 100(24): 14351-6. PubMed ID: 14623972
544.  (APP) (PUR) Jgoun, A. A.; Eldarov, M. A.; Solodar, L. I.; Sokolov, N. N.; Archakov, A. I.; Skryabin, K. G. (2001) Heterologous expression of eukaryotic cytochromes P450 1. Heterologous expression of cytochrome P450 2B4 in E.coli as fusion proteins with different affiniti purification tags. Voprosy Meditsinskoj Khimii 47(4): 391-392.
545.  (REL) Johansson, D. G.; Macao, B.; Sandberg, A.; Hard, T. (2008) SEA domain autoproteolysis accelerated by conformational strain: mechanistic aspects. J Mol Biol 377(4): 1130-43. PubMed ID: 18314133
546.  (REL) (REV) Johnson, R. L.; Scott, M. P. (1998) New players and puzzles in the Hedgehog signaling pathway. Curr Opin Genet Dev. 8(4): 450-6.. PubMed ID: 9729722
547.  (REL) (REV) Johnson, E. C.; Kent, S. B. (2006) Insights into the mechanism and catalysis of the native chemical ligation reaction. J Am Chem Soc 128(20): 6640-6. PubMed ID: 16704265
548.  (INT) (NEB) (NMR) (Structure) Johnson, M. A.; Southworth, M. W.; Herrmann, T.; Brace, L.; Perler, F. B.; Wuthrich, K. (2007) NMR structure of a KlbA intein precursor from Methanococcus jannaschii. Protein Sci 16(7): 1316-28. PubMed ID: 17586768
549.  (INT) (NEB) (NMR) (Structure) Johnson, M. A.; Southworth, M. W.; Perler, F. B.; Wuthrich, K. (2007B) NMR assignment of a KlbA intein precursor from Methanococcus jannaschii. Biomol NMR Assig 1: 19-21.
550.  (APP) (REV) Johnsson, N.; Johnsson, K. (2003) A fusion of disciplines: Chemical approaches to exploit fusion proteins for functional genomics. Chembiochem 4(9): 803-810.
551.  (INT) (REL) Jordan, A.; Torrents, E.; Jeanthon, C.; Eliasson, R.; Hellman, U.; Wernstedt, C.; Barbe, J.; Gibert, I.; Reichard, P. (1997) B12-dependent ribonucleotide reductases from deeply rooted eubacteria are structurally related to the aerobic enzyme from Escherichia coli. Proc Natl Acad Sci U S A. 94(25): 13487-92.. PubMed ID: 9391052
552.  (EN) (REV) Jurica, M. S.; Stoddard, B. L. (1999) Homing endonucleases: structure, function and evolution. Cell Mol Life Sci 55(10): 1304-26. PubMed ID: 10487208
553.  (REL) Kaihara, A.; Kawai, Y.; Sato, M.; Ozawa, T.; Umezawa, Y. (2003) Locating a protein-protein interaction in living cells via split Renilla luciferase complementation. Anal Chem 75(16): 4176-81. PubMed ID: 14632132
554.  (APP) (INT) (REL) Kalia, J.; Raines, R. T. (2006) Reactivity of intein thioesters: appending a functional group to a protein. Chembiochem 7(9): 1375-83. PubMed ID: 16897799
555.  (APP) (EPL) (TAG) Kalia, J.; Abbott, N. L.; Raines, R. T. (2007) General method for site-specific protein immobilization by Staudinger ligation. Bioconjug Chem 18(4): 1064-9. PubMed ID: 17488064
556.  (APP) (EPL) (TAG) Kallenberger, B. C.; Love, J. D.; Chatterjee, V. K.; Schwabe, J. W. (2003) A dynamic mechanism of nuclear receptor activation and its perturbation in a human disease. Nat Struct Biol. 10(2): 136-40.. PubMed ID: 12536206
557.  (INT) Kane, P. M.; Yamashiro, C. T.; Wolczyk, D. F.; Neff, N.; Goebl, M.; Stevens, T. H. (1990) Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase. Science 250(4981): 651-7. PubMed ID: 2146742
558.  (INT) Kaneko, T.; Tanaka, A.; Sato, S.; Kotani, H.; Sazuka, T.; Miyajima, N.; Sugiura, M.; Tabata, S. (1995) Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome. DNA Res. 2(4): 153-66, 191-98.. PubMed ID: 8590279
559.  (INT) Kaneko, T.; Sato, S.; Kotani, H.; Tanaka, A.; Asamizu, E.; Nakamura, Y.; Miyajima, N.; Hirosawa, M.; Sugiura, M.; Sasamoto, S.; Kimura, T.; Hosouchi, T.; Matsuno, A.; Muraki, A.; Nakazaki, N.; Naruo, K.; Okumura, S.; Shimpo, S.; Takeuchi, C.; Wada, T.; Watanabe, A.; Yamada, M.; Yasuda, M.; Tabata, S. (1996) Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions. DNA Res. 3(3): 109-36.. PubMed ID: 8905231
560.  (INT) Kaneko, T.; Sato, S.; Kotani, H.; Tanaka, A.; Asamizu, E.; Nakamura, Y.; Miyajima, N.; Hirosawa, M.; Sugiura, M.; Sasamoto, S.; Kimura, T.; Hosouchi, T.; Matsuno, A.; Muraki, A.; Nakazaki, N.; Naruo, K.; Okumura, S.; Shimpo, S.; Takeuchi, C.; Wada, T.; Watanabe, A.; Yamada, M.; Yasuda, M.; Tabata, S. (1996B) Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions (supplement). DNA Res. 3(3): 185-209.. PubMed ID: 8905238
561.  (INT) Kaneko, T.; Tabata, S. (1997) Complete genome structure of the unicellular cyanobacterium Synechocystis sp. PCC6803. Plant Cell Physiol. 38(11): 1171-6.. PubMed ID: 9435137
562.  (INT) Kaneko, T.; Nakamura, Y.; Wolk, C. P.; Kuritz, T.; Sasamoto, S.; Watanabe, A.; Iriguchi, M.; Ishikawa, A.; Kawashima, K.; Kimura, T.; Kishida, Y.; Kohara, M.; Matsumoto, M.; Matsuno, A.; Muraki, A.; Nakazaki, N.; Shimpo, S.; Sugimoto, M.; Takazawa, M.; Yamada, M.; Yasuda, M.; Tabata, S. (2001) Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. DNA Res. 8(5): 205-13; 227-53.. PubMed ID: 11759840
563.  (INT) Kaneko, T.; Nakajima, N.; Okamoto, S.; Suzuki, I.; Tanabe, Y.; Tamaoki, M.; Nakamura, Y.; Kasai, F.; Watanabe, A.; Kawashima, K.; Kishida, Y.; Ono, A.; Shimizu, Y.; Takahashi, C.; Minami, C.; Fujishiro, T.; Kohara, M.; Katoh, M.; Nakazaki, N.; Nakayama, S.; Yamada, M.; Tabata, S.; Watanabe, M. M. (2007) Complete genomic structure of the bloom-forming toxic cyanobacterium Microcystis aeruginosa NIES-843. DNA Res 14(6): 247-56. PubMed ID: 18192279
564.  (APP) (TS) Kanno, A.; Ozawa, T.; Umezawa, Y. (2006) Intein-mediated reporter gene assay for detecting protein-protein interactions in living mammalian cells. Anal Chem 78(2): 556-60. PubMed ID: 16408939
565.  (APP) (CPS) (TAG) (TS) Kanno, A.; Ozawa, T.; Umezawa, Y. (2006B) Genetically encoded optical probe for detecting release of proteins from mitochondria toward cytosol in living cells and mammals. Anal Chem 78(23): 8076-81. PubMed ID: 17134142
566.  (APP) (CYCL) (TAG) Kanno, A.; Yamanaka, Y.; Hirano, H.; Umezawa, Y.; Ozawa, T. (2007) Cyclic luciferase for real-time sensing of caspase-3 activities in living mammals. Angew Chem Int Ed Engl 46(40): 7595-9. PubMed ID: 17722214
567.  (APP) (CPS) (TAG) (TS) Kanno, A.; Ozawa, T.; Umezawa, Y. (2009) Bioluminescent imaging of MAPK function with intein-mediated reporter gene assay. Methods Mol Biol 574: 185-92. PubMed ID: 19685309
568.  (APP) (EPL) Karow, A. R.; Theissen, B.; Klostermeier, D. (2007) Authentic interdomain communication in an RNA helicase reconstituted by expressed protein ligation of two helicase domains. Febs J 274(2): 463-73. PubMed ID: 17229151
569.  (APP) (PUR) Karring, H.; Bjornsson, A.; Thirup, S.; Clark, B. F.; Knudsen, C. R. (2003) Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts. Eur J Biochem 270(21): 4294-305. PubMed ID: 14622294
570.  (APP) (EPL) (TAG) Karukurichi, K. R.; Wang, L.; Uzasci, L.; Manlandro, C. M.; Wang, Q.; Cole, P. A. (2010) Analysis of p300/CBP histone acetyltransferase regulation using circular permutation and semisynthesis. J Am Chem Soc 132(4): 1222-3. PubMed ID: 20063892
571.  (APP) (EPL) Katayama, H.; Hojo, H.; Ohira, T.; Ishii, A.; Nozaki, T.; Goto, K.; Nakahara, Y.; Takahashi, T.; Hasegawa, Y.; Nagasawa, H. (2010) Correct disulfide pairing is required for the biological activity of crustacean androgenic gland hormone (AGH): synthetic studies of AGH. Biochemistry 49(8): 1798-807. PubMed ID: 20092253
572.  (APP) (EPL) (PUR) Katragadda, M.; Lambris, J. D. (2006) Expression of compstatin in Escherichia coli: Incorporation of unnatural amino acids enhances its activity. Protein Expr Purif 47(1): 289-95. PubMed ID: 16406678
573.  (INT) Kawarabayasi, Y.; Sawada, M.; Horikawa, H.; Haikawa, Y.; Hino, Y.; Yamamoto, S.; Sekine, M.; Baba, S.; Kosugi, H.; Hosoyama, A.; Nagai, Y.; Sakai, M.; Ogura, K.; Otsuka, R.; Nakazawa, H.; Takamiya, M.; Ohfuku, Y.; Funahashi, T.; Tanaka, T.; Kudoh, Y.; Yamazaki, J.; Kushida, N.; Oguchi, A.; Aoki, K.; Kikuchi, H. (1998) Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3. DNA Res 5(2): 55-76. PubMed ID: 9679194
574.  (INT) Kawarabayasi, Y.; Sawada, M.; Horikawa, H.; Haikawa, Y.; Hino, Y.; Yamamoto, S.; Sekine, M.; Baba, S.; Kosugi, H.; Hosoyama, A.; Nagai, Y.; Sakai, M.; Ogura, K.; Otsuka, R.; Nakazawa, H.; Takamiya, M.; Ohfuku, Y.; Funahashi, T.; Tanaka, T.; Kudoh, Y.; Yamazaki, J.; Kushida, N.; Oguchi, A.; Aoki, K.; Kikuchi, H. (1998B) Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3 (supplement). DNA Res. 5(2): 147-55.. PubMed ID: 9679203
575.  (INT) Kawarabayasi, Y.; Hino, Y.; Horikawa, H.; Yamazaki, S.; Haikawa, Y.; Jin-no, K.; Takahashi, M.; Sekine, M.; Baba, S.; Ankai, A.; Kosugi, H.; Hosoyama, A.; Fukui, S.; Nagai, Y.; Nishijima, K.; Nakazawa, H.; Takamiya, M.; Masuda, S.; Funahashi, T.; Tanaka, T.; Kudoh, Y.; Yamazaki, J.; Kushida, N.; Oguchi, A.; Kikuchi, H.; et al., (1999) Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1. DNA Res. 6(2): 83-101, 145-52.. PubMed ID: 10382966
576.  (INT) Kawarabayasi, Y. (2001) Genome of Pyrococcus horikoshii OT3. Methods Enzymol. 330: 124-34.. PubMed ID: 11210494
577.  (INT) Kawasaki, M.; Makino, S.; Matsuzawa, H.; Satow, Y.; Ohya, Y.; Anraku, Y. (1996) Folding-dependent in vitro protein splicing of the Saccharomyces cerevisiae VMA1 protozyme. Biochem Biophys Res Commun 222(3): 827-32. PubMed ID: 8651930
578.  (EN) (INT) Kawasaki, M.; Nogami, S.; Satow, Y.; Ohya, Y.; Anraku, Y. (1997) Identification of three core regions essential for protein splicing of the yeast Vma1 protozyme. A random mutagenesis study of the entire Vma1-derived endonuclease sequence. J Biol Chem 272(25): 15668-74. PubMed ID: 9188457
579.  (INT) Kawasaki, M.; Satow, Y.; Ohya, Y.; Anraku, Y. (1997B) Protein splicing in the yeast Vma1 protozyme: evidence for an intramolecular reaction. FEBS Lett 412(3): 518-20. PubMed ID: 9276458
580.  (INT) Kawashima, T.; Yamamoto, Y.; Aramaki, H.; Nunoshiba, T.; Kawamoto, T.; Watanabe, K.,; Yamazaki, M.; Kanehori, K.; Amano, N.; Ohya, Y.; Makino, K.; Suzuki, M. (1999) Determination of the complete genomic DNA sequence of Thermoplasma volvanium GSS1. Proc. Jpn. Acad 75: 213-218.
581.  (EN) (REV) Keeling, P. J.; Roger, A. J. (1995) The selfish pursuit of sex. Nature. 375(6529): 283.. PubMed ID: 7753189
582.  (APP) (PUR) (TS) Kempe, K.; Rubtsova, M.; Gils, M. (2009) Intein-mediated protein assembly in transgenic wheat: production of active barnase and acetolactate synthase from split genes. Plant Biotechnol J 7(3): 283-97. PubMed ID: 19222807
583.  (EPL) (REL) (REV) Kent, S. B. (2009) Total chemical synthesis of proteins. Chem Soc Rev 38(2): 338-51. PubMed ID: 19169452
584.  (INT) Kerrigan, A. M.; Powers, T. L.; Dorval, D. M.; Reitter, J. N.; Mills, K. V. (2009) Protein splicing of the three Pyrococcus abyssi ribonucleotide reductase inteins. Biochem Biophys Res Commun 387(1): 153-7. PubMed ID: 19577540
585.  (INT) Kerrigan, Adam M.; Powers, Taryn L.; Dorval, Deirdre M.; Reitter, Julie N.; Mills, Kenneth V. (2009B) The dependence of three P. abyssi inteins on extein sequence for efficient protein splicing. FASEB J._MeetingAbstracts 22(1_MeetingAbstracts): 611.3.
586.  (INT) Kerrigan, Adam M.; Powers, Taryn L.; Dorval, Deirdre M.; Reitter, Julie N.; Mills, Kenneth V. (2009C) The influence of extein residues on the protein splicing of three Pyrococcus abyssi inteins. FASEB J._MeetingAbstracts 23(1_MeetingAbstracts): 502.3.
587.  (APP) (REV) (TS) Khan, M. S.; Khalid, A. M.; Malik, K. A. (2005) Intein-mediated protein trans-splicing and transgene containment in plastids. Trends Biotechnol 23(5): 217-20. PubMed ID: 15865996
588.  (APP) (EPL) (REV) Khmelnitsky, Yuri L. (2004) Current strategies for in vitro protein glycosylation. Journal of Molecular Catalysis B: Enzymatic 31(4-6): 73-81.
589.  (APP) (PUR) Khodak, Y. A.; Koroleva, O. N.; Drutsa, V. L. (2007) A System for Heterologous Expression and Isolation of Escherichia coli RNA Polymerase and Its Components. Biochemistry (Mosc) 72(2): 178-87. PubMed ID: 17367295
590.  (APP) (PUR) (Structure) Kim, J. J.; Rho, S. H.; Im, Y. J.; Kim, E. J.; Eom, S. H. (2001) Crystallization and preliminary X-ray diffraction studies of the guanylate kinase-like domain of PSD-95 protein from rat. Acta Crystallogr D Biol Crystallogr 57(Pt 4): 616-7. PubMed ID: 11264600
591.  (APP) (TAG) (TS) Kim, S. B.; Ozawa, T.; Watanabe, S.; Umezawa, Y. (2004) High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase. Proc Natl Acad Sci U S A 101(32): 11542-7. PubMed ID: 15289615
592.  (APP) (TAG) (TS) Kim, S. B.; Ozawa, T.; Umezawa, Y. (2005) Genetically encoded stress indicator for noninvasively imaging endogenous corticosterone in living mice. Anal Chem 77(20): 6588-93. PubMed ID: 16223244
593.  (APP) (TAG) (TS) Kim, S. B.; Takao, R.; Ozawa, T.; Umezawa, Y. (2005B) Quantitative determination of protein nuclear transport induced by phosphorylation or by proteolysis. Anal Chem 77(21): 6928-34. PubMed ID: 16255591
594.  (APP) (TAG) (TS) Kim, S. B.; Ozawa, T.; Umezawa, Y. (2005C) A genetically encoded indicator for assaying bioactive chemicals that induce nuclear transport of glucocorticoid receptor. Anal Biochem 347(2): 213-20. PubMed ID: 16269125
595.  (INT) Kim, Y. J.; Lee, H. S.; Bae, S. S.; Jeon, J. H.; Lim, J. K.; Cho, Y.; Nam, K. H.; Kang, S. G.; Kim, S. J.; Kwon, S. T.; Lee, J. H. (2007) Cloning, purification, and characterization of a new DNA polymerase from a hyperthermophilic archaeon, Thermococcus sp. NA1. J Microbiol Biotechnol 17(7): 1090-7. PubMed ID: 18051318
596.  (APP) (PUR) Kim, G. A.; Lee, M. S.; Sun, Y.; Lee, B. D.; Lee, J. I.; Lee, J. H.; Kwon, S. T. (2008) Characterization of cold-active uracil-DNA glycosylase from Bacillus sp. HJ171 and its use for contamination control in PCR. Appl Microbiol Biotechnol 80(5): 785-94. PubMed ID: 18626641
597.  (APP) (EPL) (TAG) Kim, J.; Guermah, M.; McGinty, R. K.; Lee, J. S.; Tang, Z.; Milne, T. A.; Shilatifard, A.; Muir, T. W.; Roeder, R. G. (2009) RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells. Cell 137(3): 459-71. PubMed ID: 19410543
598.  (APP) (EPL) (TAG) Kim, S.; Sun, H.; Ball, H. L.; Wassmann, K.; Luo, X.; Yu, H. (2010) Phosphorylation of the spindle checkpoint protein Mad2 regulates its conformational transition. Proc Natl Acad Sci U S A (IN PRESS). PubMed ID: 21041666
599.  (APP) (EPL) (REV) Kimmerlin, T.; Seebach, D. (2005) '100 years of peptide synthesis': ligation methods for peptide and protein synthesis with applications to beta-peptide assemblies. J Pept Res 65(2): 229-60. PubMed ID: 15705167
600.  (APP) (CYCL) (EPL) (REV) Kimura, R.; Camarero, J. A. (2005) Expressed protein ligation: a new tool for the biosynthesis of cyclic polypeptides. Protein Pept Lett 12(8): 789-94. PubMed ID: 16305550
601.  (APP) (CYCL) Kimura, R. H.; Tran, A. T.; Camarero, J. A. (2006) Biosynthesis of the cyclotide kalata B1 by using protein splicing. Angew Chem Int Ed Engl 45(6): 973-6. PubMed ID: 16381044
602.  (APP) (CYCL) (PUR) Kinsella, T. M.; Ohashi, C. T.; Harder, A. G.; Yam, G. C.; Li, W.; Peelle, B.; Pali, E. S.; Bennett, M. K.; Molineaux, S. M.; Anderson, D. A.; Masuda, E. S.; Payan, D. G. (2002) Retrovirally delivered random cyclic Peptide libraries yield inhibitors of interleukin-4 signaling in human B cells. J Biol Chem 277(40): 37512-8. PubMed ID: 12167667
603.  (APP) Kinsland, C.; Taylor, S. V.; Kelleher, N. L.; McLafferty, F. W.; Begley, T. P. (1998) Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis. Protein Sci 7(8): 1839-42. PubMed ID: 10082383
604.  (APP) (PUR) Kiraly, O.; Guan, L.; Szepessy, E.; Toth, M.; Kukor, Z.; Sahin-Toth, M. (2006) Expression of human cationic trypsinogen with an authentic N terminus using intein-mediated splicing in aminopeptidase P deficient Escherichia coli. Protein Expr Purif 48(1): 104-11. PubMed ID: 16542853
605.  (APP) (PUR) Kirkland, B. H.; Keyhani, N. O. (2010) Expression and purification of a functionally active class I fungal hydrophobin from the entomopathogenic fungus Beauveria bassiana in E. coli. J Ind Microbiol Biotechnol (IN PRESS). PubMed ID: 20640587
606.  (APP) (PUR) Kiss, R. S.; Weers, P. M.; Narayanaswami, V.; Cohen, J.; Kay, C. M.; Ryan, R. O. (2003) Structure-guided protein engineering modulates helix bundle exchangeable apolipoprotein properties. J Biol Chem 278(24): 21952-9. PubMed ID: 12684504
607.  (INT) (Structure) Klabunde, T.; Sharma, S.; Telenti, A.; Jacobs, W. R., Jr.; Sacchettini, J. C. (1998) Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing. Nat Struct Biol 5(1): 31-6. PubMed ID: 9437427
608.  (INT) Klenk, H. P.; Clayton, R. A.; Tomb, J. F.; White, O.; Nelson, K. E.; Ketchum, K. A.; Dodson, R. J.; Gwinn, M.; Hickey, E. K.; Peterson, J. D.; Richardson, D. L.; Kerlavage, A. R.; Graham, D. E.; Kyrpides, N. C.; Fleischmann, R. D.; Quackenbush, J.; Lee, N. H.; Sutton, G. G.; Gill, S.; Kirkness, E. F.; Dougherty, B. A.; McKenney, K.; Adams, M. D.; Loftus, B.; Peterson, S.; Reich, C. I.; McNeil, L. K.; Badger, J. H.; Glodek, A.; Zhou, L.; Overbeek, R.; Gocayne, J. D.; Weidman, J. F.; McDonald, L.; Utterback, T.; Cotton, M. D.; Spriggs, T.; Artiach, P.; Kaine, B. P.; Sykes, S. M.; Sadow, P. W.; D'Andrea, K. P.; Bowman, C.; Fujii, C.; Garland, S. A.; Mason, T. M.; Olsen, G. J.; Fraser, C. M.; Smith, H. O.; Woese, C. R.; Venter, J. C. (1997) The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus. Nature 390(6658): 364-70. PubMed ID: 9389475
609.  (EN) (REV) Kobayashi, I. (2001) Behavior of restriction-modification systems as selfish mobile elements and their impact on genome evolution. Nucleic Acids Res 29(18): 3742-56. PubMed ID: 11557807
610.  (PUR) Kochanowski, R.; Kotlowski, R.; Szweda, P. (2007) Expression and intein-mediated purification of novel staphylokinase SakSTAR with reduced immunogenicity and antiplatelet and antithrombin activation. Appl Biochem Biotechnol 141(2-3): 321-33. PubMed ID: 18025559
611.  (APP) (REV) Kochendoerfer, G. G.; Kent, S. B. (1999) Chemical protein synthesis. Curr Opin Chem Biol 3(6): 665-71. PubMed ID: 10600727
612.  (APP) (EPL) (REL) (REV) Kochendoerfer, G. G. (2001) Chemical protein synthesis methods in drug discovery. Curr Opin Drug Discov Devel. 4(2): 205-14.. PubMed ID: 11378960
613.  (APP) (EPL) (REL) (REV) Kochendoerfer, G. (2003) Chemical and biological properties of polymer-modified proteins. Expert Opin Biol Ther. 3(8): 1253-61.. PubMed ID: 14640951
614.  (APP) (NMR) (REL) (Structure) Kochendoerfer, G. G.; Jones, D. H.; Lee, S.; Oblatt-Montal, M.; Opella, S. J.; Montal, M. (2004) Functional characterization and NMR spectroscopy on full-length Vpu from HIV-1 prepared by total chemical synthesis. J Am Chem Soc. 126(8): 2439-46.. PubMed ID: 14982452
615.  (APP) (EPL) (REL) (REV) Kochendoerfer, G. G. (2005) Site-specific polymer modification of therapeutic proteins. Curr Opin Chem Biol. 9(6): 555-60. Epub 2005 Oct 17.. PubMed ID: 16233987
616.  (APP) (EPL) (NEB) Kochinyan, S.; Sun, L.; Ghosh, I.; Barshevsky, T.; Xu, J.; Xu, M. Q. (2007) Use of intein-mediated phosphoprotein arrays to study substrate specificity of protein phosphatases. Biotechniques 42(1): 63-9. PubMed ID: 17269486
617.  (APP) (EPL) (REV) Koeller, K. M.; Wong, C. H. (2000) Complex carbohydrate synthesis tools for glycobiologists: enzyme-based approach and programmable one-pot strategies. Glycobiology. 10(11): 1157-69.. PubMed ID: 11087708
618.  (APP) (EPL) (REV) Koeller, K. M.; Wong, C. H. (2000B) Synthesis of complex carbohydrates and glycoconjugates: enzyme-based and programmable one-pot strategies. Chem Rev. 100(12): 4465-94.. PubMed ID: 11749355
619.  (APP) (EPL) (REV) (TAG) Komarov, A. G.; Linn, K. M.; Devereaux, J. J.; Valiyaveetil, F. I. (2009) Semisynthesis of K+ channels. Methods Enzymol 462: 135-50. PubMed ID: 19632473
620.  (EN) (INT) Komori, K.; Fujita, N.; Ichiyanagi, K.; Shinagawa, H.; Morikawa, K.; Ishino, Y. (1999) PI-PfuI and PI-PfuII, intein-coded homing endonucleases from Pyrococcus furiosus. I. Purification and identification of the homing-type endonuclease activities. Nucleic Acids Res 27(21): 4167-74. PubMed ID: 10518607
621.  (EN) (INT) Komori, K.; Ichiyanagi, K.; Morikawa, K.; Ishino, Y. (1999B) PI-PfuI and PI-PfuII, intein-coded homing endonucleases from Pyrococcus furiosus. II. Characterization Of the binding and cleavage abilities by site-directed mutagenesis. Nucleic Acids Res 27(21): 4175-82. PubMed ID: 10518608
622.  (INT) Koonin, E. V. (1995) A protein splice-junction motif in hedgehog family proteins. Trends Biochem Sci. 20(4): 141-2.. PubMed ID: 7770912
623.  (INT) (REL) Koonin, E. V.; Aravind, L.; Kondrashov, A. S. (2000) The impact of comparative genomics on our understanding of evolution. Cell. 101(6): 573-6.. PubMed ID: 10892642
624.  (APP) (EPL) (TAG) Korona, Dagmara; Korona, Boguslawa; Wojcik, Edward (2009) Intein-mediated protein splicing of human Eg5 kinesin. FASEB J._MeetingAbstracts 23(1_MeetingAbstracts): 502.19.
625.  (EN) (INT) Koufopanou, V.; Goddard, M. R.; Burt, A. (2002) Adaptation for horizontal transfer in a homing endonuclease. Mol Biol Evol 19(3): 239-46. PubMed ID: 11861883
626.  (EN) (REV) Kowalski, J. C.; Derbyshire, V. (2002) Characterization of homing endonucleases. Methods 28(3): 365-73. PubMed ID: 12431440
627.  (APP) (CYCL) Kritzer, J. A.; Hamamichi, S.; McCaffery, J. M.; Santagata, S.; Naumann, T. A.; Caldwell, K. A.; Caldwell, G. A.; Lindquist, S. (2009) Rapid selection of cyclic peptides that reduce alpha-synuclein toxicity in yeast and animal models. Nat Chem Biol 5(9): 655-63. PubMed ID: 19597508
628.  (EN) (REV) Kuhlmann, U. C.; Moore, G. R.; James, R.; Kleanthous, C.; Hemmings, A. M. (1999) Structural parsimony in endonuclease active sites: should the number of homing endonuclease families be redefined?. FEBS Lett 463(1-2): 1-2. PubMed ID: 10601625
629.  (INT) Kumar, R. A.; Vaze, M. B.; Chandra, N. R.; Vijayan, M.; Muniyappa, K. (1996) Functional characterization of the precursor and spliced forms of RecA protein of Mycobacterium tuberculosis. Biochemistry 35(6): 1793-802. PubMed ID: 8639660
630.  (APP) (EPL) (PUR) Kumar Bhat, R.; Berger, S. (2007) New and easy strategy for cloning, expression, purification, and characterization of the 5S subunit of transcarboxylase from Propionibacterium f. shermanii. Prep Biochem Biotechnol 37(1): 13-26. PubMed ID: 17134979
631.  (APP) (PUR) Kunanopparat, A.; Chaivisuthangkura, P.; Senapin, S.; Longyant, S.; Rukpratanporn, S.; Flegel, T. W.; Sithigorngul, P. (2010) Detection of infectious myonecrosis virus using monoclonal antibody specific to N and C fragments of the capsid protein expressed heterologously. J Virol Methods (IN PRESS). PubMed ID: 21029750
632.  (APP) (PUR) Kunes, Y. Z.; Gion, W. R.; Fung, E.; Salfeld, J. G.; Zhu, R. R.; Sakorafas, P.; Carson, G. R. (2009) Expression of antibodies using single-open reading frame vector design and polyprotein processing from mammalian cells. Biotechnol Prog 25(3): 735-44. PubMed ID: 19363814
633.  (INT) Kunst, F.; Ogasawara, N.; Moszer, I.; Albertini, A. M.; Alloni, G.; Azevedo, V.; Bertero, M. G.; Bessieres, P.; Bolotin, A.; Borchert, S.; Borriss, R.; Boursier, L.; Brans, A.; Braun, M.; Brignell, S. C.; Bron, S.; Brouillet, S.; Bruschi, C. V.; Caldwell, B.; Capuano, V.; Carter, N. M.; Choi, S. K.; Codani, J. J.; Connerton, I. F.; Danchin, A.; et al., (1997) The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature 390(6657): 249-56. PubMed ID: 9384377
634.  (APP) (TAG) (TS) Kurpiers, T.; Mootz, H. D. (2007) Regioselective Cysteine Bioconjugation by Appending a Labeled Cystein Tag to a Protein by Using Protein Splicing in trans. Angew Chem Int Ed Engl 46(27): 5234-5237. PubMed ID: 17538921
635.  (APP) (PUR) (TAG) (TS) Kurpiers, T.; Mootz, H. D. (2008) Site-specific chemical modification of proteins with a prelabelled cysteine tag using the artificially split Mxe GyrA intein. Chembiochem 9(14): 2317-25. PubMed ID: 18756552
636.  (APP) (PUR) (REV) (TAG) Kusnezow, W.; Hoheisel, J. D. (2003) Solid supports for microarray immunoassays. J Mol Recognit 16(4): 165-76. PubMed ID: 12898667
637.  (APP) (PUR) Kutchma, A. J.; Hoang, T. T.; Schweizer, H. P. (1999) Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (FabD). J Bacteriol 181(17): 5498-504. PubMed ID: 10464226
638.  (INT) Kwan, T.; Liu, J.; DuBow, M.; Gros, P.; Pelletier, J. (2005) The complete genomes and proteomes of 27 Staphylococcus aureus bacteriophages. Proc Natl Acad Sci U S A. 102(14): 5174-9. Epub 2005 Mar 23.. PubMed ID: 15788529
639.  (APP) (TAG) (TS) Kwon, Y.; Coleman, M. A.; Camarero, J. A. (2006) Selective Immobilization of Proteins onto Solid Supports through Split-Intein-Mediated Protein Trans-Splicing. Angew Chem Int Ed Engl 45(11): 1726-1729. PubMed ID: 16470557
640.  (APP) (CYCL) (TS) Kwon, J. S.; Bal, J.; Hwang, H. M.; Kim, J. Y. (2008) A circularly permuted beta-lactamase as a novel reporter for evaluation of protein cyclization efficiency. J Microbiol 46(4): 456-61. PubMed ID: 18758738
641.  (APP) (PUR) Lacey, B. M.; Hondal, R. J. (2006) Characterization of mitochondrial thioredoxin reductase from C. elegans. Biochem Biophys Res Commun 346(3): 629-36. PubMed ID: 16780799
642.  (APP) (PUR) (TAG) Laitem, C.; Choul-Li, S.; Baillat, D.; Begue, A.; Aumercier, M. (2008) Efficient system for biotinylated recombinant Ets-1 production in Escherichiacoli: a useful tool for studying interactions between Ets-1 and its partners. Protein Expr Purif 62(1): 53-63. PubMed ID: 18639639
643.  (REL) (REV) Lambowitz, A. M.; Belfort, M. (1993) Introns as mobile genetic elements. Annu Rev Biochem 62: 587-622. PubMed ID: 8352597
644.  (APP) (REV) (TAG) Lay, P. T.; Yao, S. Q. (2005) Intein-mediated, in vitro and in vivo protein modifications with small molecules. Protein and Peptide Letters 12(8): 769-775.
645.  (INT) Lazarevic, V.; Soldo, B.; Dusterhoft, A.; Hilbert, H.; Mauel, C.; Karamata, D. (1998) Introns and intein coding sequence in the ribonucleotide reductase genes of Bacillus subtilis temperate bacteriophage SPbeta. Proc Natl Acad Sci U S A 95(4): 1692-7. PubMed ID: 9465078
646.  (INT) Lazarevic, V. (2001) Ribonucleotide reductase genes of Bacillus prophages: a refuge to introns and intein coding sequences. Nucleic Acids Res 29(15): 3212-8. PubMed ID: 11470879
647.  (INT) Lecompte, O.; Ripp, R.; Puzos-Barbe, V.; Duprat, S.; Heilig, R.; Dietrich, J.; Thierry, J. C.; Poch, O. (2001) Genome evolution at the genus level: comparison of three complete genomes of hyperthermophilic archaea. Genome Res. 11(6): 981-93.. PubMed ID: 11381026
648.  (REL) Lee, J. J.; Ekker, S. C.; von Kessler, D. P.; Porter, J. A.; Sun, B. I.; Beachy, P. A. (1994) Autoproteolysis in hedgehog protein biogenesis. Science. 266(5190): 1528-37.. PubMed ID: 7985023
649.  (APP) (PUR) (Structure) (TAG) Lee, N. Y.; Hazlett, T. L.; Koland, J. G. (2006) Structure and dynamics of the epidermal growth factor receptor C-terminal phosphorylation domain. Protein Sci 15(5): 1142-52. PubMed ID: 16597832
650.  (APP) (PUR) Lee, M. S.; Kim, G. A.; Seo, M. S.; Lee, J. H.; Kwon, S. T. (2009) Characterization of heat-labile uracil-DNA glycosylase from Psychrobacter sp. HJ147 and its application to the polymerase chain reaction. Biotechnol Appl Biochem 52(Pt 2): 167-75. PubMed ID: 18412541
651.  (APP) (EPL) (TAG) Lesaicherre, M. L.; Lue, R. Y.; Chen, G. Y.; Zhu, Q.; Yao, S. Q. (2002) Intein-mediated biotinylation of proteins and its application in a protein microarray. J Am Chem Soc 124(30): 8768-9. PubMed ID: 12137509
652.  (APP) (REV) Lesley, S. A. (2001) High-throughput proteomics: protein expression and purification in the postgenomic world. Protein Expr Purif 22(2): 159-64. PubMed ID: 11437590
653.  (APP) (EPL) Levengood, M. R.; Kerwood, C. C.; Chatterjee, C.; van der Donk, W. A. (2009) Investigation of the substrate specificity of lacticin 481 synthetase by using nonproteinogenic amino acids. Chembiochem 10(5): 911-9. PubMed ID: 19222036
654.  (APP) (CPS) (TS) Lew, B. M.; Mills, K. V.; Paulus, H. (1998) Protein splicing in vitro with a semisynthetic two-component minimal intein. J Biol Chem 273(26): 15887-90. PubMed ID: 9632632
655.  (APP) (CPS) (INT) (TS) Lew, B. M.; Mills, K. V.; Paulus, H. (1999) Characteristics of protein splicing in trans mediated by a semisynthetic split intein. Biopolymers 51(5): 355-62. PubMed ID: 10685046
656.  (APP) (CPS) Lew, B. M.; Paulus, H. (2002) An in vivo screening system against protein splicing useful for the isolation of non-splicing mutants or inhibitors of the RecA intein of Mycobacterium tuberculosis. Gene 282(1-2): 169-77. PubMed ID: 11814689
657.  (REL) Li, Y.; Zhao, Y.; Hatfield, S.; Wan, R.; Zhu, Q.; Li, X.; McMills, M.; Ma, Y.; Li, J.; Brown, K. L.; He, C.; Liu, F.; Chen, X. (2000) Dipeptide seryl-histidine and related oligopeptides cleave DNA, protein, and a carboxyl ester. Bioorg Med Chem 8(12): 2675-80. PubMed ID: 11131157
658.  (APP) (PUR) Li, H. H.; Thomas, M. J.; Pan, W.; Alexander, E.; Samuel, M.; Sorci-Thomas, M. G. (2001) Preparation and incorporation of probe-labeled apoA-I for fluorescence resonance energy transfer studies of rHDL. J Lipid Res 42(12): 2084-91. PubMed ID: 11734582
659.  (APP) (PUR) Li, Z. Y.; Fan, J. H.; Yuan, J. M. (2002) Single-column purification of recombinant human neurotrophin-3 (hNT3) by using the intein mediated self-cleaving system. Biotechnology Letters 24(20): 1723-1727.
660.  (APP) (CPS) (TS) Li, J.; Sun, W.; Wang, B.; Xiao, X.; Liu, X. Q. (2008) Protein trans-splicing as a means for viral vector-mediated in vivo gene therapy. Hum Gene Ther 19(9): 958-64. PubMed ID: 18788906
661.  (APP) (EPL) (TAG) Li, J.; Taylor, I. A.; Lloyd, J.; Clapperton, J. A.; Howell, S.; MacMillan, D.; Smerdon, S. J. (2008B) Chk2 oligomerization studied by phosphopeptide ligation: implications for regulation and phosphodependent interactions. J Biol Chem 283(51): 36019-30. PubMed ID: 18948271
662.  (EN) Li, H.; Pellenz, S.; Ulge, U.; Stoddard, B. L.; Monnat, R. J., Jr. (2009) Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins. Nucleic Acids Res 37(5): 1650-62. PubMed ID: 19153140
663.  (APP) (EPL) (REL) (TAG) Li, X.; Fekner, T.; Ottesen, J. J.; Chan, M. K. (2009) A pyrrolysine analogue for site-specific protein ubiquitination. Angew Chem Int Ed Engl 48(48): 9184-7. PubMed ID: 19882608
664.  (APP) (CPS) (PUR) Liang, R.; Liu, X.; Liu, J.; Ren, Q.; Liang, P.; Lin, Z.; Xie, X. (2007) A T7-expression system under temperature control could create temperature-sensitive phenotype of target gene in Escherichia coli. J Microbiol Methods 68(3): 497-506. PubMed ID: 17169451
665.  (APP) (PUR) (REV) Lichty, J. J.; Malecki, J. L.; Agnew, H. D.; Michelson-Horowitz, D. J.; Tan, S. (2005) Comparison of affinity tags for protein purification. Protein Expr Purif. 41(1): 98-105.. PubMed ID: 15802226
666.  (APP) (EPL) (REV) Lin, Michael Z.; Wang, Lei (2008) Selective Labeling of Proteins with Chemical Probes in Living Cells. Physiology 23(3): 131-141.
667.  (APP) (CYCL) (PUR) (TS) Lin, Z.; Wang, S.; Yang, X.; Yang, K. (2008) [Construction and evaluation of DnaB split intein high expression vector and a six amino acids cyclic peptide library]. Sheng Wu Gong Cheng Xue Bao 24(11): 1924-30. PubMed ID: 19256340
668.  (REL) (REV) Little, J. W. (1993) LexA cleavage and other self-processing reactions. J Bacteriol. 175(16): 4943-50.. PubMed ID: 8349538
669.  (INT) Liu, X. Q.; Hu, Z. (1997) Identification and characterization of a cyanobacterial DnaX intein. FEBS Lett 408(3): 311-4. PubMed ID: 9188783
670.  (EN) (INT) Liu, X. Q.; Hu, Z. (1997B) A DnaB intein in Rhodothermus marinus: indication of recent intein homing across remotely related organisms. Proc Natl Acad Sci U S A 94(15): 7851-6. PubMed ID: 9223276
671.  (INT) (REV) Liu, X. Q. (2000) Protein-splicing intein: Genetic mobility, origin, and evolution. Annu Rev Genet 34: 61-76. PubMed ID: 11092822
672.  (APP) (PUR) Liu, C.; Shim, J. H.; Benkovic, S. J. (2000B) The Unexpected Catalytic Properties of a Heterodimer of GAR Transformylase. Bioorg Chem 28(6): 316-323.
673.  (INT) (TS) Liu, X. Q.; Yang, J. (2003) Split dnaE genes encoding multiple novel inteins in Trichodesmium erythraeum. J Biol Chem 278(29): 26315-8. PubMed ID: 12766150
674.  (APP) (CYCL) (PUR) Liu, C. W.; Corboy, M. J.; DeMartino, G. N.; Thomas, P. J. (2003) Endoproteolytic activity of the proteasome. Science 299(5605): 408-11. PubMed ID: 12481023
675.  (INT) Liu, X. Q.; Yang, J.; Meng, Q. (2003B) Four inteins and three group II introns encoded in a bacterial ribonucleotide reductase gene. J Biol Chem 278(47): 46826-31. PubMed ID: 12975359
676.  (EN) (INT) Liu, X. Q.; Yang, J. (2004) Prp8 intein in fungal pathogens: target for potential antifungal drugs. FEBS Lett 572(1-3): 46-50. PubMed ID: 15304322
677.  (INT) Liu, X. Q.; Yang, J. (2004B) Bacterial thymidylate synthase with intein, group II Intron, and distinctive ThyX motifs. J Bacteriol 186(18): 6316-9. PubMed ID: 15342603
678.  (APP) (EPL) Liu, D.; Xu, R.; Dutta, K.; Cowburn, D. (2008) N-terminal cysteinyl proteins can be prepared using thrombin cleavage. FEBS Lett 582(7): 1163-7. PubMed ID: 18331839
679.  (APP) (PUR) Liu, J. R.; Duan, C. H.; Zhao, X.; Tzen, J. T.; Cheng, K. J.; Pai, C. K. (2008B) Cloning of a rumen fungal xylanase gene and purification of the recombinant enzyme via artificial oil bodies. Appl Microbiol Biotechnol 79(2): 225-33. PubMed ID: 18415096
680.  (APP) (NMR) (REV) Liu, D.; Xu, R.; Cowburn, D. (2009) Segmental isotopic labeling of proteins for nuclear magnetic resonance. Methods Enzymol 462: 151-75. PubMed ID: 19632474
681.  (APP) (INT) (TS) Lockless, S. W.; Muir, T. W. (2009) Traceless protein splicing utilizing evolved split inteins. Proc Natl Acad Sci U S A 106(27): 10999-1004. PubMed ID: 19541616
682.  (INT) Loftus, B. J.; Fung, E.; Roncaglia, P.; Rowley, D.; Amedeo, P.; Bruno, D.; Vamathevan, J.; Miranda, M.; Anderson, I. J.; Fraser, J. A.; Allen, J. E.; Bosdet, I. E.; Brent, M. R.; Chiu, R.; Doering, T. L.; Donlin, M. J.; D'Souza, C. A.; Fox, D. S.; Grinberg, V.; Fu, J.; Fukushima, M.; Haas, B. J.; Huang, J. C.; Janbon, G.; Jones, S. J.; Koo, H. L.; Krzywinski, M. I.; Kwon-Chung, J. K.; Lengeler, K. B.; Maiti, R.; Marra, M. A.; Marra, R. E.; Mathewson, C. A.; Mitchell, T. G.; Pertea, M.; Riggs, F. R.; Salzberg, S. L.; Schein, J. E.; Shvartsbeyn, A.; Shin, H.; Shumway, M.; Specht, C. A.; Suh, B. B.; Tenney, A.; Utterback, T. R.; Wickes, B. L.; Wortman, J. R.; Wye, N. H.; Kronstad, J. W.; Lodge, J. K.; Heitman, J.; Davis, R. W.; Fraser, C. M.; Hyman, R. W. (2005) The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans. Science. 307(5713): 1321-4. Epub 2005 Jan 13.. PubMed ID: 15653466
683.  (APP) (EPL) (REV) (TAG) Love, K. R.; Catic, A.; Schlieker, C.; Ploegh, H. L. (2007) Mechanisms, biology and inhibitors of deubiquitinating enzymes. Nat Chem Biol 3(11): 697-705. PubMed ID: 17948018
684.  (APP) (EPL) (TAG) Lovrinovic, M.; Seidel, R.; Wacker, R.; Schroeder, H.; Seitz, O.; Engelhard, M.; Goody, R. S.; Niemeyer, C. M. (2003) Synthesis of protein-nucleic acid conjugates by expressed protein ligation. Chem Commun (Camb) (7): 822-3. PubMed ID: 12739629
685.  (APP) (EPL) (TAG) Lovrinovic, M.; Niemeyer, C. M. (2005) Rapid synthesis of DNA-cysteine conjugates for expressed protein ligation. Biochem Biophys Res Commun 335(3): 943-8. PubMed ID: 16102730
686.  (APP) (EPL) (TAG) Lovrinovic, M.; Spengler, M.; Deutsch, C.; Niemeyer, C. M. (2005B) Synthesis of covalent DNA-protein conjugates by expressed protein ligation. Mol Biosyst 1(1): 64-9. PubMed ID: 16880965
687.  (APP) (EPL) (PUR) (TAG) Lovrinovic, M.; Niemeyer, C. M. (2007) Microtiter Plate-Based Screening for the Optimization of DNA-Protein Conjugate Synthesis by Means of Expressed Protein Ligation. Chembiochem 8(1): 61-67. PubMed ID: 17121402
688.  (APP) (EPL) (TAG) Lovrinovic, M.; Fruk, L.; Schroder, H.; Niemeyer, C. M. (2007B) Site-specific labeling of DNA-protein conjugates by means of expressed protein ligation. Chem Commun (Camb) 4: 353-355. PubMed ID: 17220968
689.  (APP) (EPL) Lu, W.; Gong, D.; Bar-Sagi, D.; Cole, P. A. (2001) Site-specific incorporation of a phosphotyrosine mimetic reveals a role for tyrosine phosphorylation of SHP-2 in cell signaling. Mol Cell 8(4): 759-69. PubMed ID: 11684012
690.  (APP) (EPL) Lu, W.; Shen, K.; Cole, P. A. (2003) Chemical dissection of the effects of tyrosine phosphorylation of SHP-2. Biochemistry 42(18): 5461-8. PubMed ID: 12731888
691.  (APP) (REL) Lu, M.; Min, T.; Eliezer, D.; Wu, H. (2006) Native chemical ligation in covalent caspase inhibition by p35. Chem Biol 13(2): 117-22. PubMed ID: 16492559
692.  (APP) (EPL) Lu, X.; Olsen, S. K.; Capili, A. D.; Cisar, J. S.; Lima, C. D.; Tan, D. S. (2010) Designed semisynthetic protein inhibitors of ub/ubl e1 activating enzymes. J Am Chem Soc 132(6): 1748-9. PubMed ID: 20099854
693.  (APP) (PUR) Lu, Shuo; Zgurskaya, Helen I (2010) Reconstitution of macrolide pump MacAB-TolC into lipid nanodiscs. FASEB J. 24(1_MeetingAbstracts): 699.3-.
694.  (APP) (PUR) Lu, M.; Ngo, W.; Mei, Y.; Munshi, V.; Burlein, C.; Loughran, M. H.; Williams, P. D.; Hazuda, D. J.; Miller, M. D.; Grobler, J. A.; Diamond, T. L.; Lai, M. T. (2010B) Purification of untagged HIV-1 reverse transcriptase by affinity chromatography. Protein Expr Purif 71(2): 231-239. PubMed ID: 20060474
695.  (EN) Lucas, P.; Otis, C.; Mercier, J. P.; Turmel, M.; Lemieux, C. (2001) Rapid evolution of the DNA-binding site in LAGLIDADG homing endonucleases. Nucleic Acids Res. 29(4): 960-9.. PubMed ID: 11160929
696.  (APP) (PUR) (TS) Ludwig, C.; Pfeiff, M.; Linne, U.; Mootz, H. D. (2006) Ligation of a synthetic peptide to the N terminus of a recombinant protein using semisynthetic protein trans-splicing. Angew Chem Int Ed Engl 45(31): 5218-21. PubMed ID: 16823791
697.  (CPS) (INT) (TS) Ludwig, C.; Schwarzer, D.; Mootz, H. D. (2008) Interaction studies and alanine scanning analysis of a semi-synthetic split intein reveal thiazoline ring formation from an intermediate of the protein splicing reaction. J Biol Chem 283(37): 25264-72. PubMed ID: 18625708
698.  (APP) (TS) Ludwig, C.; Schwarzer, D.; Zettler, J.; Garbe, D.; Janning, P.; Czeslik, C.; Mootz, H. D. (2009) Semisynthesis of proteins using split inteins. Methods Enzymol 462: 77-96. PubMed ID: 19632470
699.  (APP) (EPL) (TAG) Lue, R. Y.; Chen, G. Y.; Hu, Y.; Zhu, Q.; Yao, S. Q. (2004) Versatile protein biotinylation strategies for potential high-throughput proteomics. J Am Chem Soc 126(4): 1055-62. PubMed ID: 14746473
700.  (APP) (EPL) (TAG) Lue, R. Y.; Chen, G. Y.; Zhu, Q.; Lesaicherre, M. L.; Yao, S. Q. (2004B) Site-specific immobilization of biotinylated proteins for protein microarray analysis. Methods Mol Biol 264: 85-100. PubMed ID: 15020782
701.  (APP) (PUR) Luka, Z.; Wagner, C. (2003) Expression and purification of glycine N-methyltransferases in Escherichia coli. Protein Expr Purif 28(2): 280-6. PubMed ID: 12699692
702.  (INT) Luo, J.; Hall, B. D. (2007) A multistep process gave rise to RNA polymerase IV of land plants. J Mol Evol 64(1): 101-12. PubMed ID: 17160640
703.  (APP) (PUR) Luttkopf, D.; Muller, U.; Skov, P. S.; Ballmer-Weber, B. K.; Wuthrich, B.; Skamstrup Hansen, K.; Poulsen, L. K.; Kastner, M.; Haustein, D.; Vieths, S. (2002) Comparison of four variants of a major allergen in hazelnut (Corylus avellana) Cor a 1.04 with the major hazel pollen allergen Cor a 1.01. Mol Immunol 38(7): 515-25. PubMed ID: 11750653
704.  (REL) (REV) Lutz, S.; Benkovic, S. J. (2000) Homology-independent protein engineering. Curr Opin Biotechnol. 11(4): 319-24.. PubMed ID: 10975450
705.  (EN) Lykke-Andersen, J.; Garrett, R. A.; Kjems, J. (1996) Protein footprinting approach to mapping DNA binding sites of two archaeal homing enzymes: evidence for a two-domain protein structure. Nucleic Acids Res 24(20): 3982-9. PubMed ID: 8918801
706.  (APP) Lykke-Andersen, J.; Christiansen, J. (1998) The C-terminal carboxy group of T7 RNA polymerase ensures efficient magnesium ion-dependent catalysis. Nucleic Acids Res 26(24): 5630-5. PubMed ID: 9837993
707.  (APP) (PUR) Ma, J.; Cooney, C. L. (2004) Application of vortex flow adsorption technology to intein-mediated recovery of recombinant human alpha1-antitrypsin. Biotechnol Prog 20(1): 269-76. PubMed ID: 14763852
708.  (APP) (PUR) Ma, C.; Gao, M.; Liu, W.; Zhu, J.; Tian, H.; Gao, X.; Yao, W. (2010) Intein-mediated expression and purification of an analog of glucagon-like peptide-1 in Escherichia coli. Protein Pept Lett 17(10): 1245-50. PubMed ID: 20441562
709.  (APP) (EPL) (TAG) Maag, D.; Lorsch, J. R. (2003) Communication between eukaryotic translation initiation factors 1 and 1A on the yeast small ribosomal subunit. J Mol Biol 330(5): 917-24. PubMed ID: 12860115
710.  (APP) (EPL) (TAG) Maag, D.; Fekete, C. A.; Gryczynski, Z.; Lorsch, J. R. (2005) A conformational change in the eukaryotic translation preinitiation complex and release of eIF1 signal recognition of the start codon. Mol Cell 17(2): 265-75. PubMed ID: 15664195
711.  (APP) (PUR) Machova, Z.; Muhle, C.; Krauss, U.; Trehin, R.; Koch, A.; Merkle, H. P.; Beck-Sickinger, A. G. (2002) Cellular internalization of enhanced green fluorescent protein ligated to a human calcitonin-based carrier peptide. Chembiochem 3(7): 672-7. PubMed ID: 12325002
712.  (APP) (EPL) Machova, Z.; Von Eggelkraut-Gottanka, R.; Wehofsky, N.; Bordusa, F.; Beck-Sickinger, A. G. (2003) Expressed Enzymatic Ligation for the Semisynthesis of Chemically Modified Proteins. Angewandte Chemie - International Edition 42(40): 4916-4918.
713.  (APP) (EPL) (REV) Machova, Z.; Beck-Sickinger, A. G. (2005) Expressed protein ligation for protein semisynthesis and engineering. Methods Mol Biol 298: 105-30. PubMed ID: 16044543
714.  (APP) (EPL) (REV) Macmillan, Derek; Bertozzi, Carolyn R. (2000) New Directions in Glycoprotein Engineering. Tetrahedron 56(48): 9515-9525.
715.  (APP) (EPL) Macmillan, D.; Arham, L. (2004) Cyanogen bromide cleavage generates fragments suitable for expressed protein and glycoprotein ligation. J Am Chem Soc 126(31): 9530-1. PubMed ID: 15291543
716.  (APP) (EPL) Macmillan, D.; Bertozzi, C. R. (2004B) Modular assembly of glycoproteins: towards the synthesis of GlyCAM-1 by using expressed protein ligation. Angew Chem Int Ed Engl 43(11): 1355-9. PubMed ID: 15368405
717.  (INT) MacNeill, S. A. (2009) The haloarchaeal chromosome replication machinery. Biochem Soc Trans 37(Pt 1): 108-13. PubMed ID: 19143612
718.  (INT) Maeder, D. L.; Weiss, R. B.; Dunn, D. M.; Cherry, J. L.; Gonzalez, J. M.; DiRuggiero, J.; Robb, F. T. (1999) Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences. Genetics 152(4): 1299-305. PubMed ID: 10430560
719.  (APP) (EPL) (REV) Magliery, T. J. (2005) Unnatural protein engineering: Producing proteins with unnatural amino acids. Medicinal Chemistry Reviews - Online 2(4): 303-323.
720.  (INT) Makarova, K. S.; Aravind, L.; Wolf, Y. I.; Tatusov, R. L.; Minton, K. W.; Koonin, E. V.; Daly, M. J. (2001) Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics. Microbiol Mol Biol Rev. 65(1): 44-79.. PubMed ID: 11238985
721.  (APP) (PUR) Malki, A.; Kern, R.; Abdallah, J.; Richarme, G. (2003) Characterization of the Escherichia coli YedU protein as a molecular chaperone. Biochem Biophys Res Commun 301(2): 430-6. PubMed ID: 12565879
722.  (PUR) Malone, C. L.; Boles, B. R.; Horswill, A. R. (2007) Biosynthesis of Staphylococcus aureus autoinducing peptides by using the synechocystis DnaB mini-intein. Appl Environ Microbiol 73(19): 6036-44. PubMed ID: 17693565
723.  (APP) (PUR) Manconi, B.; Cabras, T.; Vitali, A.; Fanali, C.; Fiorita, A.; Inzitari, R.; Castagnola, M.; Messana, I.; Sanna, M. T. (2010) Expression, purification, phosphorylation and characterization of recombinant human statherin. Protein Expr Purif 69(2): 219-25. PubMed ID: 19651217
724.  (REL) (REV) Mann, R. K.; Beachy, P. A. (2000) Cholesterol modification of proteins. Biochim Biophys Acta. 1529(1-3): 188-202.. PubMed ID: 11111088
725.  (REL) (REV) Mann, R. K.; Beachy, P. A. (2004) Novel lipid modifications of secreted protein signals. Annu Rev Biochem 73: 891-923. PubMed ID: 15189162
726.  (APP) (EPL) (TAG) Manohar, M.; Mooney, A. M.; North, J. A.; Nakkula, R. J.; Picking, J. W.; Edon, A.; Fishel, R.; Poirier, M. G.; Ottesen, J. J. (2009) Acetylation of histone H3 at the nucleosome dyad alters DNA-histone binding. J Biol Chem 284(35): 23312-21. PubMed ID: 19520870
727.  (APP) (PUR) Manrique, J. M.; Affranchino, J. L.; Gonzalez, S. A. (2008) In vitro binding of simian immunodeficiency virus matrix protein to the cytoplasmic domain of the envelope glycoprotein. Virology 374(2): 273-9. PubMed ID: 18255119
728.  (REL) Mao, H.; Hart, S. A.; Schink, A.; Pollok, B. A. (2004) Sortase-mediated protein ligation: a new method for protein engineering. J Am Chem Soc 126(9): 2670-1. PubMed ID: 14995162
729.  (APP) (EN) (REV) Marcaida, M. J.; Munoz, I. G.; Blanco, F. J.; Prieto, J.; Montoya, G. (2010) Homing endonucleases: from basics to therapeutic applications. Cell Mol Life Sci 67(5): 727-48. PubMed ID: 19915993
730.  (INT) Mardanov, A. V.; Ravin, N. V.; Svetlitchnyi, V. A.; Beletsky, A. V.; Miroshnichenko, M. L.; Bonch-Osmolovskaya, E. A.; Skryabin, K. G. (2009) Metabolic versatility and indigenous origin of the archaeon Thermococcus sibiricus, isolated from a siberian oil reservoir, as revealed by genome analysis. Appl Environ Microbiol 75(13): 4580-8. PubMed ID: 19447963
731.  (INT) Marsic, D.; Flaman, J. M.; Ng, J. D. (2008) New DNA polymerase from the hyperthermophilic marine archaeon Thermococcus thioreducens. Extremophiles 12(6): 775-88. PubMed ID: 18670731
732.  (INT) (NEB) (TS) Martin, D. D.; Xu, M. Q.; Evans, T. C., Jr. (2001) Characterization of a naturally occurring trans-splicing intein from Synechocystis sp. PCC6803. Biochemistry 40(5): 1393-402. PubMed ID: 11170467
733.  (APP) (REL) (REV) Massoud, T. F.; Paulmurugan, R.; De, A.; Ray, P.; Gambhir, S. S. (2007) Reporter gene imaging of protein-protein interactions in living subjects. Current Opinion in Biotechnology 18(1): 31-37.
734.  (APP) (EPL) (NEB) (PUR) Mathys, S.; Evans, T. C.; Chute, I. C.; Wu, H.; Chong, S.; Benner, J.; Liu, X. Q.; Xu, M. Q. (1999) Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. Gene 231(1-2): 1-13. PubMed ID: 10231563
735.  (INT) Matrat, S.; Petrella, S.; Cambau, E.; Sougakoff, W.; Jarlier, V.; Aubry, A. (2007) Expression and purification of an active form of the Mycobacterium leprae DNA gyrase and its inhibition by quinolones. Antimicrob Agents Chemother 51(5): 1643-8. PubMed ID: 17325221
736.  (EN) (INT) (Structure) Matsumura, H.; Takahashi, H.; Inoue, T.; Yamamoto, T.; Hashimoto, H.; Nishioka, M.; Fujiwara, S.; Takagi, M.; Imanaka, T.; Kai, Y. (2006) Crystal structure of intein homing endonuclease II encoded in DNA polymerase gene from hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1. Proteins 63(3): 711-715. PubMed ID: 16493661
737.  (INT) Mattes, T. E.; Alexander, A. K.; Richardson, P. M.; Munk, A. C.; Han, C. S.; Stothard, P.; Coleman, N. V. (2008) The genome of Polaromonas sp. strain JS666: insights into the evolution of a hydrocarbon- and xenobiotic-degrading bacterium, and features of relevance to biotechnology. Appl Environ Microbiol 74(20): 6405-16. PubMed ID: 18723656
738.  (APP) (PUR) Matthews, Trey; Baumann, Richard G.; Domanski, Tammy L. (2007) Analysis and Characterization of Staphylococcus aureus ClpB and Associated Co-Chaperone Proteins dnaK, dnaJ and grpE. FASEB J. 21(6): A1025-b.
739.  (INT) (REL) Matus, D. Q.; Magie, C. R.; Pang, K.; Martindale, M. Q.; Thomsen, G. H. (2008) The Hedgehog gene family of the cnidarian, Nematostella vectensis, and implications for understanding metazoan Hedgehog pathway evolution. Dev Biol 313(2): 501-18. PubMed ID: 18068698
740.  (APP) (EPL) McGinty, R. K.; Kim, J.; Chatterjee, C.; Roeder, R. G.; Muir, T. W. (2008) Chemically ubiquitylated histone H2B stimulates hDot1L-mediated intranucleosomal methylation. Nature 453(7196): 812-6. PubMed ID: 18449190
741.  (APP) (EPL) (REV) (TAG) McGinty, R. K.; Chatterjee, C.; Muir, T. W. (2009) Semisynthesis of ubiquitylated proteins. Methods Enzymol 462: 225-43. PubMed ID: 19632477
742.  (APP) (EPL) (TAG) McGinty, R. K.; Kohn, M.; Chatterjee, C.; Chiang, K. P.; Pratt, M. R.; Muir, T. W. (2009) Structure-activity analysis of semisynthetic nucleosomes: mechanistic insights into the stimulation of Dot1L by ubiquitylated histone H2B. ACS Chem Biol 4(11): 958-68. PubMed ID: 19799466
743.  (APP) (CYCL) McMurray, J. S.; Budde, R. J.; Ke, S.; Obeyesekere, N. U.; Wang, W.; Ramdas, L.; Lewis, C. A. (1998) Cyclic peptides as probes of the substrate binding site of the cytosolic tyrosine kinase, pp60c-src. Arch Biochem Biophys. 355(1): 124-30.. PubMed ID: 9647675
744.  (APP) (PUR) Mee, C.; Banki, M. R.; Wood, D. W. (2008) Towards the elimination of chromatography in protein purification: Expressing proteins engineered to purify themselves. Chemical Engineering Journal 135(1-2): 56-62.
745.  (EN) (INT) Meng, Q.; Zhang, Y.; Liu, X. Q. (2007) Rare group I intron with insertion sequence element in a bacterial ribonucleotide reductase gene. J Bacteriol 189(5): 2150-4. PubMed ID: 17172330
746.  (APP) (PUR) Miao, J.; Wu, W.; Spielmann, T.; Belfort, M.; Derbyshire, V.; Belfort, G. (2005) Single-step affinity purification of toxic and non-toxic proteins on a fluidics platform. Lab Chip 5(3): 248-53. PubMed ID: 15726200
747.  (APP) (REV) Michnick, S. W. (2001) Exploring protein interactions by interaction-induced folding of proteins from complementary peptide fragments. Curr Opin Struct Biol. 11(4): 472-7.. PubMed ID: 11495741
748.  (INT) (REV) Milchert, L. E.; Liberles, D. A.; Elofsson, A. (2002) The salmon genome (and other issues in bioinformatics). Genome Biol. 3(7): REPORTS4022. Epub 2002 Jun 24.. PubMed ID: 12184805
749.  (APP) (REL) Milic, J.; Seidel, R.; Becker, C. F.; Goody, R. S.; Engelhard, M. (2008) Semisynthesis of H-Ras with a glutamic acid methylester at position 61. Biopolymers 90(3): 399-405. PubMed ID: 17599776
750.  (APP) (EPL) Miller, J. S.; Dudkin, V. Y.; Lyon, G. J.; Muir, T. W.; Danishefsky, S. J. (2003) Toward fully synthetic N-linked glycoproteins. Angew Chem Int Ed Engl. 42(4): 431-4.. PubMed ID: 12569509
751.  (APP) (REV) Miller, L. W.; Cornish, V. W. (2005) Selective chemical labeling of proteins in living cells. Curr Opin Chem Biol 9(1): 56-61. PubMed ID: 15701454
752.  (CPS) (TS) Mills, K. V.; Lew, B. M.; Jiang, S.; Paulus, H. (1998) Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA intein. Proc Natl Acad Sci U S A 95(7): 3543-8. PubMed ID: 9520402
753.  (CPS) (TS) Mills, K. V.; Paulus, H. (2001) Reversible inhibition of protein splicing by zinc ion. J Biol Chem 276(14): 10832-8. PubMed ID: 11152694
754.  (INT) Mills, K. V.; Manning, J. S.; Garcia, A. M.; Wuerdeman, L. A. (2004) Protein splicing of a Pyrococcus abyssi intein with a C-terminal glutamine. J Biol Chem 279(20): 20685-91. PubMed ID: 15024006
755.  (INT) Mills, K. V.; Dorval, D. M.; Lewandowski, K. T. (2005) Kinetic analysis of the individual steps of protein splicing for the Pyrococcus abyssi PolII intein. J Biol Chem 280(4): 2714-20. PubMed ID: 15557319
756.  (NEB) (REV) Mills, K. V.; Perler, F. B. (2005B) The mechanism of intein-mediated protein splicing: variations on a theme. Protein Pept Lett 12(8): 751-5. PubMed ID: 16305544
757.  (INT) (REV) Mills, K.V.; Paulus, H. (2005C) Biochemical mechanisms of intein-mdiated protein splicing. In: Homing Endonucleases and Inteins, ed. Belfort, M.; Derbyshire, V.; Stoddard, B.L.; Wood, D.W.. (Springer-Verlag, Heidelberg, Germany) Vol. 16 pages 233-255.
758.  (APP) (CPS) (PUR) Mills, K. V.; Connor, K. R.; Dorval, D. M.; Lewandowski, K. T. (2006) Protein purification via temperature-dependent, intein-mediated cleavage from an immobilized metal affinity resin. Anal Biochem 356(1): 86-93. PubMed ID: 16756933
759.  (INT) Mills, Kenneth V.; Reitter, Julie N.; Duffee, Lauren R.; York, Daniel J.; Dorval, Deirdre M.; Lewandowski, Katherine T. (2010) Non-canonical inteins: Alternate mechanisms for protein splicing. FASEB J. 24(1_MeetingAbstracts): 463.12-.
760.  (REL) Min, W.; Jones, D. H. (1994) In vitro splicing of concanavalin A is catalyzed by asparaginyl endopeptidase. Nat Struct Biol. 1(8): 502-4.. PubMed ID: 7664074
761.  (APP) (PUR) Min, B.; Pelaschier, J. T.; Graham, D. E.; Tumbula-Hansen, D.; Soll, D. (2002) Transfer RNA-dependent amino acid biosynthesis: an essential route to asparagine formation. Proc Natl Acad Sci U S A 99(5): 2678-83. PubMed ID: 11880622
762.  (APP) (EPL) Minakhin, L.; Camarero, J. A.; Holford, M.; Parker, C.; Muir, T. W.; Severinov, K. (2001) Mapping the molecular interface between the sigma(70) subunit of E. coli RNA polymerase and T4 AsiA. J Mol Biol. 306(4): 631-42.. PubMed ID: 11243776
763.  (REL) Miranda, L. P.; Alewood, P. F. (1999) Accelerated chemical synthesis of peptides and small proteins. Proc Natl Acad Sci U S A. 96(4): 1181-6.. PubMed ID: 9989998
764.  (APP) (EPL) (REV) Miranda, L. P.; Alewood, P. F. (2000) Challenges for protein chemical synthesis in the 21st century: bridging genomics and proteomics. Biopolymers. 55(3): 217-26.. PubMed ID: 11074416
765.  (EN) (INT) Miyamoto, S.; Mizutani, R.; Satow, Y.; Kawasaki, M.; Ohya, Y.; Anraku, Y. (1999) Recognition and cleavage of double-stranded DNA by yeast VMA1-derived endonuclease. Nucleic Acids Symp Ser. (42): 197-8.. PubMed ID: 10780447
766.  (INT) (Structure) Mizutani, R.; Nogami, S.; Kawasaki, M.; Ohya, Y.; Anraku, Y.; Satow, Y. (2002) Protein-splicing reaction via a thiazolidine intermediate: crystal structure of the VMA1-derived endonuclease bearing the N and C-terminal propeptides. J Mol Biol 316(4): 919-29. PubMed ID: 11884132
767.  (INT) (Structure) Mizutani, R.; Anraku, Y.; Satow, Y. (2004) Protein splicing of yeast VMA1-derived endonuclease via thiazolidine intermediates. J Synchrotron Radiat 11(Pt 1): 109-12. PubMed ID: 14646148
768.  (INT) (REV) Mizutani, R.; Satow, Y.; Anraku, Y. (2004B) [Discovery, analysis and chemical mechanism of protein splicing]. Tanpakushitsu Kakusan Koso 49(1): 18-27. PubMed ID: 14748135
769.  (INT) Mongodin, E. F.; Nelson, K. E.; Daugherty, S.; Deboy, R. T.; Wister, J.; Khouri, H.; Weidman, J.; Walsh, D. A.; Papke, R. T.; Sanchez Perez, G.; Sharma, A. K.; Nesbo, C. L.; MacLeod, D.; Bapteste, E.; Doolittle, W. F.; Charlebois, R. L.; Legault, B.; Rodriguez-Valera, F. (2005) The genome of Salinibacter ruber: convergence and gene exchange among hyperhalophilic bacteria and archaea. Proc Natl Acad Sci U S A 102(50): 18147-52. PubMed ID: 16330755
770.  (INT) Monot, M.; Honore, N.; Garnier, T.; Zidane, N.; Sherafi, D.; Paniz-Mondolfi, A.; Matsuoka, M.; Taylor, G. M.; Donoghue, H. D.; Bouwman, A.; Mays, S.; Watson, C.; Lockwood, D.; Khamesipour, A.; Dowlati, Y.; Jianping, S.; Rea, T. H.; Vera-Cabrera, L.; Stefani, M. M.; Banu, S.; Macdonald, M.; Sapkota, B. R.; Spencer, J. S.; Thomas, J.; Harshman, K.; Singh, P.; Busso, P.; Gattiker, A.; Rougemont, J.; Brennan, P. J.; Cole, S. T. (2009) Comparative genomic and phylogeographic analysis of Mycobacterium leprae. Nat Genet 41(12): 1282-9. PubMed ID: 19881526
771.  (APP) (CPS) (TS) Mootz, H. D.; Muir, T. W. (2002) Protein splicing triggered by a small molecule. J Am Chem Soc 124(31): 9044-5. PubMed ID: 12148996
772.  (APP) (CPS) (TS) Mootz, H. D.; Blum, E. S.; Tyszkiewicz, A. B.; Muir, T. W. (2003) Conditional protein splicing: a new tool to control protein structure and function in vitro and in vivo. J Am Chem Soc 125(35): 10561-9. PubMed ID: 12940738
773.  (APP) (CPS) (TS) Mootz, H. D.; Blum, E. S.; Muir, T. W. (2004) Activation of an autoregulated protein kinase by conditional protein splicing. Angew Chem Int Ed Engl 43(39): 5189-92. PubMed ID: 15455415
774.  (APP) (REV) Mootz, H. D. (2007) Posttranslational processing: Protein splicing by inteins - Goldmine for protein chemical applications. Posttranslationale Prozessierung: Protein-spleiflen Durch Inteine Fund - Grube fu?r Proteinchemische Anwendungen 13(2): 165-167.
775.  (APP) (REV) (TS) Mootz, H. D. (2009) Split inteins as versatile tools for protein semisynthesis. Chembiochem 10(16): 2579-89. PubMed ID: 19708049
776.  (APP) (PUR) Morassutti, C.; De Amicis, F.; Skerlavaj, B.; Zanetti, M.; Marchetti, S. (2002) Production of a recombinant antimicrobial peptide in transgenic plants using a modified VMA intein expression system. FEBS Lett 519(1-3): 141-6. PubMed ID: 12023033
777.  (APP) (PUR) Morassutti, C.; De Amicis, F.; Bandiera, A.; Marchetti, S. (2005) Expression of SMAP-29 cathelicidin-like peptide in bacterial cells by intein-mediated system. Protein Expr Purif 39(2): 160-8. PubMed ID: 15642466
778.  (APP) (PUR) Morgan, C. T.; Tsivkovskii, R.; Kosinsky, Y. A.; Efremov, R. G.; Lutsenko, S. (2004) The distinct functional properties of the nucleotide-binding domain of ATP7B, the human copper-transporting ATPase: analysis of the Wilson disease mutations E1064A, H1069Q, R1151H, and C1104F. J Biol Chem 279(35): 36363-71. PubMed ID: 15205462
779.  (APP) (PUR) Morizane, Y.; Honda, R.; Fukami, K.; Yasuda, H. (2005) X-linked inhibitor of apoptosis functions as ubiquitin ligase toward mature caspase-9 and cytosolic Smac/DIABLO. J Biochem 137(2): 125-32. PubMed ID: 15749826
780.  (APP) (EPL) (REV) Moroder, L. (2005) Isosteric replacement of sulfur with other chalcogens in peptides and proteins. J Pept Sci 11(4): 187-214. PubMed ID: 15782428
781.  (APP) (PUR) Morris, S. K.; Harkins, T. T.; Tennyson, R. B.; Lindsley, J. E. (1999) Kinetic and thermodynamic analysis of mutant type II DNA topoisomerases that cannot covalently cleave DNA. J Biol Chem 274(6): 3446-52. PubMed ID: 9920889
782.  (EN) (INT) (Structure) Moure, C. M.; Gimble, F. S.; Quiocho, F. A. (2002) Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence. Nat Struct Biol 9(10): 764-70. PubMed ID: 12219083
783.  (EN) (Structure) Moure, C. M.; Gimble, F. S.; Quiocho, F. A. (2003) The crystal structure of the gene targeting homing endonuclease I-SceI reveals the origins of its target site specificity. Journal of Molecular Biology 334(4): 685-695.
784.  (EN) (INT) (REV) (Structure) Moure, C.M.; Quiocho, F.A. (2005) The Structure and Function of Intein-Associated Homing Endonucleases. In: Homing Endonucleases and Inteins, ed. Belfort, M.; Derbyshire, V.; Stoddard, B.L.; Wood, D.W.. (Springer-Verlag, Heidelberg, Germany) Vol. 16 pages 257-272.
785.  (EN) (Structure) Moure, C. M.; Gimble, F. S.; Quiocho, F. A. (2008) Crystal structures of I-SceI complexed to nicked DNA substrates: Snapshots of intermediates along the DNA cleavage reaction pathway. Nucleic Acids Research 36(10): 3287-3296.
786.  (EN) (REV) Mueller, J.E.; Bryk, M.; Loizos, N.; Belfort, M. (1994) Homing Endonucleases. In: Nucleases, ed. Linn, S.M.; Lloyd, R.S.; Roberts, R.J.. (Cold Spring Harbor Press, Cold Spring Harbor) pages 111-143.
787.  (REL) (REV) Muir, T. W.; Dawson, P. E.; Kent, S. B. (1997) Protein synthesis by chemical ligation of unprotected peptides in aqueous solution. Methods Enzymol. 289: 266-98.. PubMed ID: 9353726
788.  (APP) (EPL) Muir, T. W.; Sondhi, D.; Cole, P. A. (1998) Expressed protein ligation: a general method for protein engineering. Proc Natl Acad Sci U S A 95(12): 6705-10. PubMed ID: 9618476
789.  (APP) (EPL) (REV) Muir, T.W. (2001) Development and Application of Expressed Protein Ligation. Syn Lett 6: 733-740.
790.  (APP) (EPL) (REV) Muir, T. W. (2001B) Learning the Language of Chemical Biology. Chem & Eng News 13: 289.
791.  (APP) (EPL) (REV) Muir, T. W. (2003) Semisynthesis of proteins by expressed protein ligation. Annu Rev Biochem 72: 249-89. PubMed ID: 12626339
792.  (APP) (EPL) (REV) Muir, T. W. (2005) 2005 Irving Sigal Young Investigator Award. Protein Sci. 14(12): 3140-4.. PubMed ID: 16322585
793.  (APP) (REV) Muir, T. W. (2006) Chemical biology: cutting out the middle man. Nature 442(7102): 517-8. PubMed ID: 16885971
794.  (APP) (EPL) (REV) (Structure) Muir, T. W. (2008) Studying protein structure and function using semisynthesis. Biopolymers 90(6): 743-50. PubMed ID: 18924136
795.  (APP) (REV) Muir, T. W. (2009) Chemical biology in a time of transition. ACS Chem Biol 4(4): 241-3. PubMed ID: 19371132
796.  (INT) Mujika, J. I.; Lopez, X.; Mulholland, A. J. (2009) Modeling Protein Splicing: Reaction Pathway for C-Terminal Splice and Intein Scission. J Phys Chem B 113(16): 5607-5616. PubMed ID: 19326906
797.  (APP) (EPL) (TAG) Mukhopadhyay, J.; Kapanidis, A. N.; Mekler, V.; Kortkhonjia, E.; Ebright, Y. W.; Ebright, R. H. (2001) Translocation of sigma(70) with RNA polymerase during transcription: fluorescence resonance energy transfer assay for movement relative to DNA. Cell. 106(4): 453-63.. PubMed ID: 11525731
798.  (APP) (PUR) Muller, I.; Muller, R. (2006) Biochemical characterization of MelJ and MelK. Febs J 273(16): 3768-78. PubMed ID: 16911525
799.  (APP) (NMR) (TS) Muona, M.; Aranko, A. S.; Iwai, H. (2008) Segmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing. Chembiochem 9(18): 2958-61. PubMed ID: 19031436
800.  (APP) (NMR) (TS) Muona, M.; Aranko, A. S.; Raulinaitis, V.; Iwai, H. (2010) Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro. Nat Protoc 5(3): 574-87. PubMed ID: 20203672
801.  (APP) (EPL) Muralidharan, V.; Cho, J.; Trester-Zedlitz, M.; Kowalik, L.; Chait, B. T.; Raleigh, D. P.; Muir, T. W. (2004) Domain-specific incorporation of noninvasive optical probes into recombinant proteins. J Am Chem Soc 126(43): 14004-12. PubMed ID: 15506763
802.  (APP) (EPL) (REV) (TAG) (TS) Muralidharan, V.; Muir, T. W. (2006) Protein ligation: an enabling technology for the biophysical analysis of proteins. Nat Methods. 3(6): 429-38. PubMed ID: 16721376
803.  (APP) (PUR) Muratore, K. E.; Srouji, J. R.; Chow, M. A.; Kirsch, J. F. (2008) Recombinant expression of twelve evolutionarily diverse subfamily Ialpha aminotransferases. Protein Expr Purif 57(1): 34-44. PubMed ID: 17964807
804.  (APP) (EPL) Musiol, H. J.; Dong, S.; Kaiser, M.; Bausinger, R.; Zumbusch, A.; Bertsch, U.; Moroder, L. (2005) Toward semisynthetic lipoproteins by convergent strategies based on click and ligation chemistry. Chembiochem 6(4): 625-628.
805.  (APP) (PUR) Myscofski, D. M.; Dutton, E. K.; Cantor, E.; Zhang, A.; Hruby, D. E. (2001) Cleavage and purification of intein fusion proteins using the Streptococcus gordonii spex system. Prep Biochem Biotechnol 31(3): 275-90. PubMed ID: 11513092
806.  (APP) (PUR) Myung, Suwan; Wang, Yiran; Zhang, Y. H. Percival (2010) Fructose-1,6-bisphosphatase from a hyper-thermophilic bacterium Thermotoga maritima: Characterization, metabolite stability, and its implications. Process Biochemistry In Press, Corrected Proof: (IN PRESS).
807.  (INT) Nadelson, Adam C.; York, Daniel J.; Dorval, Deirdre M.; Lewandowski, Katherine T.; Connor, Katherine R.; Reitter, Julie N.; Mills, Kenneth V. (2010) Manipulation of protein splicing side-reactions to facilitate protein purification and expressed protein ligation. FASEB J. 24(1_MeetingAbstracts): 463.13-.
808.  (INT) (REL) (REV) Nagahara, N.; Matsumura, T.; Okamoto, R.; Kajihara, Y. (2009) Protein cysteine modifications: (2) Reactivity specificity and topics of medicinal chemistry and protein engineering. Current Medicinal Chemistry 16(34): 4490-4501.
809.  (EN) Nagai, Y.; Nogami, S.; Kumagai-Sano, F.; Ohya, Y. (2003) Karyopherin-mediated nuclear import of the homing endonuclease VMA1-derived endonuclease is required for self-propagation of the coding region. Molecular and Cellular Biology 23(5): 1726-1736.
810.  (EN) (INT) Nagasaki, K.; Shirai, Y.; Tomaru, Y.; Nishida, K.; Pietrokovski, S. (2005) Algal viruses with distinct intraspecies host specificities include identical intein elements. Appl Environ Microbiol 71(7): 3599-607. PubMed ID: 16000767
811.  (INT) (REV) Nagasaki, K.; Takao, Y.; Shirai, Y.; Mizumoto, H.; Tomaru, Y. (2005B) [Molecular ecology of microalgal viruses]. Uirusu 55(1): 127-32. PubMed ID: 16308539
812.  (EN) Nahon, E.; Raveh, D. (1998) Targeting a truncated HO-endonuclease of yeast to novel DNA sites with foreign zinc fingers. Nucleic Acids Res 26(5): 1233-9. PubMed ID: 9469831
813.  (INT) Nakagawa, S.; Takaki, Y.; Shimamura, S.; Reysenbach, A. L.; Takai, K.; Horikoshi, K. (2007) Deep-sea vent {varepsilon}-proteobacterial genomes provide insights into emergence of pathogens. Proc Natl Acad Sci U S A 104(29): 12146-50. PubMed ID: 17615243
814.  (INT) Nakamura, Y.; Kaneko, T.; Sato, S.; Ikeuchi, M.; Katoh, H.; Sasamoto, S.; Watanabe, A.; Iriguchi, M.; Kawashima, K.; Kimura, T.; Kishida, Y.; Kiyokawa, C.; Kohara, M.; Matsumoto, M.; Matsuno, A.; Nakazaki, N.; Shimpo, S.; Sugimoto, M.; Takeuchi, C.; Yamada, M.; Tabata, S. (2002) Complete genome structure of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. DNA Res. 9(4): 123-30.. PubMed ID: 12240834
815.  (INT) Nakamura, Y.; Kaneko, T.; Sato, S.; Ikeuchi, M.; Katoh, H.; Sasamoto, S.; Watanabe, A.; Iriguchi, M.; Kawashima, K.; Kimura, T.; Kishida, Y.; Kiyokawa, C.; Kohara, M.; Matsumoto, M.; Matsuno, A.; Nakazaki, N.; Shimpo, S.; Sugimoto, M.; Takeuchi, C.; Yamada, M.; Tabata, S. (2002B) Complete genome structure of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 (supplement). DNA Res. 9(4): 135-48.. PubMed ID: 12240836
816.  (INT) Nakamura, Y.; Kaneko, T.; Sato, S.; Mimuro, M.; Miyashita, H.; Tsuchiya, T.; Sasamoto, S.; Watanabe, A.; Kawashima, K.; Kishida, Y.; Kiyokawa, C.; Kohara, M.; Matsumoto, M.; Matsuno, A.; Nakazaki, N.; Shimpo, S.; Takeuchi, C.; Yamada, M.; Tabata, S. (2003) Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids. DNA Res. 10(4): 137-45.. PubMed ID: 14621292
817.  (INT) Nakamura, Y.; Kaneko, T.; Sato, S.; Mimuro, M.; Miyashita, H.; Tsuchiya, T.; Sasamoto, S.; Watanabe, A.; Kawashima, K.; Kishida, Y.; Kiyokawa, C.; Kohara, M.; Matsumoto, M.; Matsuno, A.; Nakazaki, N.; Shimpo, S.; Takeuchi, C.; Yamada, M.; Tabata, S. (2003B) Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids (supplement). DNA Res. 10(4): 181-201.. PubMed ID: 14621296
818.  (APP) (PUR) Nakano, S.; Zheng, G.; Nakano, M. M.; Zuber, P. (2002) Multiple pathways of Spx (YjbD) proteolysis in Bacillus subtilis. J Bacteriol 184(13): 3664-70. PubMed ID: 12057962
819.  (EN) Nakayama, H.; Morinaga, Y.; Nomura, N.; Nunoura, T.; Sako, Y.; Uchida, A. (2003C) An archaeal homing endonuclease I-PogI cleaves at the insertion site of the neighboring intron, which has no nested open reading frame. FEBS Lett 544(1-3): 165-70. PubMed ID: 12782309
820.  (APP) (CYCL) (TAG) Naumann, T. A.; Savinov, S. N.; Benkovic, S. J. (2005) Engineering an affinity tag for genetically encoded cyclic peptides. Biotechnol Bioeng 92(7): 820-30. PubMed ID: 16155946
821.  (APP) (CYCL) (TAG) Naumann, T. A.; Tavassoli, A.; Benkovic, S. J. (2008) Genetic selection of cyclic peptide Dam methyltransferase inhibitors. Chembiochem 9(2): 194-7. PubMed ID: 18085543
822.  (EN) (REV) Neff, N. F. (1993) Protein splicing: selfish genes invade cellular proteins. Curr Opin Cell Biol 5(6): 971-6. PubMed ID: 8129950
823.  (APP) (PUR) Nentwich, S. S.; Brinkrolf, K.; Gaigalat, L.; Huser, A. T.; Rey, D. A.; Mohrbach, T.; Marin, K.; Puhler, A.; Tauch, A.; Kalinowski, J. (2009) Characterization of the LacI-type transcriptional repressor RbsR controlling ribose transport in Corynebacterium glutamicum ATCC 13032. Microbiology 155(Pt 1): 150-64. PubMed ID: 19118356
824.  (INT) Ng, W. V.; Kennedy, S. P.; Mahairas, G. G.; Berquist, B.; Pan, M.; Shukla, H. D.; Lasky, S. R.; Baliga, N. S.; Thorsson, V.; Sbrogna, J.; Swartzell, S.; Weir, D.; Hall, J.; Dahl, T. A.; Welti, R.; Goo, Y. A.; Leithauser, B.; Keller, K.; Cruz, R.; Danson, M. J.; Hough, D. W.; Maddocks, D. G.; Jablonski, P. E.; Krebs, M. P.; Angevine, C. M.; Dale, H.; Isenbarger, T. A.; Peck, R. F.; Pohlschroder, M.; Spudich, J. L.; Jung, K. W.; Alam, M.; Freitas, T.; Hou, S.; Daniels, C. J.; Dennis, P. P.; Omer, A. D.; Ebhardt, H.; Lowe, T. M.; Liang, P.; Riley, M.; Hood, L.; DasSarma, S. (2000) Genome sequence of Halobacterium species NRC-1. Proc Natl Acad Sci U S A. 97(22): 12176-81.. PubMed ID: 11016950
825.  (APP) (REL) Nguyen, U. T.; Cramer, J.; Gomis, J.; Reents, R.; Gutierrez-Rodriguez, M.; Goody, R. S.; Alexandrov, K.; Waldmann, H. (2007) Exploiting the Substrate Tolerance of Farnesyltransferase for Site-Selective Protein Derivatization. Chembiochem 8(4): 408-423. PubMed ID: 17279592
826.  (CPS) (INT) (NEB) (TS) Nichols, N. M.; Benner, J. S.; Martin, D. D.; Evans, T. C., Jr. (2003) Zinc ion effects on individual Ssp DnaE intein splicing steps: regulating pathway progression. Biochemistry 42(18): 5301-11. PubMed ID: 12731871
827.  (INT) (NEB) (TS) Nichols, N. M.; Evans, T. C., Jr. (2004) Mutational analysis of protein splicing, cleavage, and self-association reactions mediated by the naturally split Ssp DnaE intein. Biochemistry 43(31): 10265-76. PubMed ID: 15287754
828.  (INT) Nie, H.; Yang, F.; Zhang, X.; Yang, J.; Chen, L.; Wang, J.; Xiong, Z.; Peng, J.; Sun, L.; Dong, J.; Xue, Y.; Xu, X.; Chen, S.; Yao, Z.; Shen, Y.; Jin, Q. (2006) Complete genome sequence of Shigella flexneri 5b and comparison with Shigella flexneri 2a. BMC Genomics 7: 173. PubMed ID: 16822325
829.  (INT) Niehaus, F.; Frey, B.; Antranikian, G. (1997) Cloning and characterisation of a thermostable alpha-DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. TY. Gene 204(1-2): 153-8. PubMed ID: 9434178
830.  (APP) (PUR) (REV) Nilsson, J.; Stahl, S.; Lundeberg, J.; Uhlen, M.; Nygren, P. A. (1997) Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expr Purif 11(1): 1-16. PubMed ID: 9325133
831.  (APP) (REL) Nilsson, B. L.; Kiessling, L. L.; Raines, R. T. (2000) Staudinger ligation: a peptide from a thioester and azide. Org Lett. 2(13): 1939-41.. PubMed ID: 10891196
832.  (APP) (REL) Nilsson, B. L.; Kiessling, L. L.; Raines, R. T. (2001) High-yielding Staudinger ligation of a phosphinothioester and azide to form a peptide. Org Lett. 3(1): 9-12.. PubMed ID: 11429880
833.  (APP) (EPL) (REL) Nilsson, B. L.; Hondal, R. J.; Soellner, M. B.; Raines, R. T. (2003) Protein assembly by orthogonal chemical ligation methods. J Am Chem Soc. 125(18): 5268-9.. PubMed ID: 12720426
834.  (APP) (EPL) (REL) (REV) Nilsson, B. L.; Soellner, M. B.; Raines, R. T. (2005) Chemical synthesis of proteins. Annu Rev Biophys Biomol Struct. 34: 91-118.. PubMed ID: 15869385
835.  (APP) (CYCL) Nilsson, L. O.; Louassini, M.; Abel-Santos, E. (2005B) Using siclopps for the discovery of novel antimicrobial peptides and their targets. Protein Pept Lett. 12(8): 795-9.. PubMed ID: 16305551
836.  (REL) (REV) Nishino, T.; Ishino, Y. (2001) [Structural motifs in DNA cleaving enzymes]. Tanpakushitsu Kakusan Koso 46(11 Suppl): 1717-25. PubMed ID: 11579571
837.  (EN) (INT) Nishioka, M.; Fujiwara, S.; Takagi, M.; Imanaka, T. (1998) Characterization of two intein homing endonucleases encoded in the DNA polymerase gene of Pyrococcus kodakaraensis strain KOD1. Nucleic Acids Res 26(19): 4409-12. PubMed ID: 9742242
838.  (EN) (Structure) Niu, Y.; Tenney, K.; Li, H.; Gimble, F. S. (2008) Engineering Variants of the I-SceI Homing Endonuclease with Strand-specific and Site-specific DNA-nicking Activity. Journal of Molecular Biology 382(1): 188-202.
839.  (EN) (INT) Noel, A. J.; Wende, W.; Pingoud, A. (2004) DNA recognition by the homing endonuclease PI-SceI involves a divalent metal ion cofactor-induced conformational change. J Biol Chem 279(8): 6794-804. PubMed ID: 14634013
840.  (INT) Nogami, S.; Satow, Y.; Ohya, Y.; Anraku, Y. (1997) Probing novel elements for protein splicing in the yeast Vma1 protozyme: a study of replacement mutagenesis and intragenic suppression. Genetics 147(1): 73-85. PubMed ID: 9286669
841.  (EN) (INT) Nogami, S.; Fukuda, T.; Nagai, Y.; Yabe, S.; Sugiura, M.; Mizutani, R.; Satow, Y.; Anraku, Y.; Ohya, Y. (2002) Homing at an extragenic locus mediated by VDE (PI-SceI) in Saccharomyces cerevisiae. Yeast 19(9): 773-82. PubMed ID: 12112231
842.  (APP) (REV) (TAG) Nolan, G. P. (2005) Tadpoles by the tail. Nat Methods 2(1): 11-2. PubMed ID: 15782163
843.  (EN) (REL) Nomura, N.; Nomura, Y.; Sussman, D.; Klein, D.; Stoddard, B. L. (2008) Recognition of a common rDNA target site in archaea and eukarya by analogous LAGLIDADG and His-Cys box homing endonucleases. Nucleic Acids Res 36(22): 6988-98. PubMed ID: 18984620
844.  (INT) Nonaka, H.; Keresztes, G.; Shinoda, Y.; Ikenaga, Y.; Abe, M.; Naito, K.; Inatomi, K.; Furukawa, K.; Inui, M.; Yukawa, H. (2006) Complete genome sequence of the dehalorespiring bacterium Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides ethenogenes 195. J Bacteriol 188(6): 2262-74. PubMed ID: 16513756
845.  (APP) (INT) (REV) Noren, C. J.; Wang, J.; Perler, F. B. (2000) Dissecting the Chemistry of Protein Splicing and Its Applications. Angew Chem Int Ed Engl 39(3): 450-466. PubMed ID: 10671234
846.  (APP) (EPL) Nyanguile, O.; Dancik, C.; Blakemore, J.; Mulgrew, K.; Kaleko, M.; Stevenson, S. C. (2003) Synthesis of adenoviral targeting molecules by intein-mediated protein ligation. Gene Ther 10(16): 1362-9. PubMed ID: 12883533
847.  (APP) (INT) (NMR) (Structure) (TS) Oeemig, J. S.; Aranko, A. S.; Djupsjobacka, J.; Heinamaki, K.; Iwai, H. (2009) Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification. FEBS Lett 583(9): 1451-6. PubMed ID: 19344715
848.  (APP) (REL) Offer, J.; Boddy, C. N.; Dawson, P. E. (2002) Extending synthetic access to proteins with a removable acyl transfer auxiliary. J Am Chem Soc. 124(17): 4642-6.. PubMed ID: 11971712
849.  (REL) Ogata, H.; Audic, S.; Barbe, V.; Artiguenave, F.; Fournier, P. E.; Raoult, D.; Claverie, J. M. (2000) Selfish DNA in protein-coding genes of Rickettsia. Science 290(5490): 347-50. PubMed ID: 11030655
850.  (INT) Ogata, H.; Raoult, D.; Claverie, J. M. (2005) A new example of viral intein in Mimivirus. Virol J 2: 8. PubMed ID: 15707490
851.  (INT) Okada, C.; Maegawa, Y.; Yao, M.; Tanaka, I. (2006) Crystal structure of an RtcB homolog protein (PH1602-extein protein) from Pyrococcus horikoshii reveals a novel fold. Proteins 63(4): 1119-22. PubMed ID: 16485279
852.  (EN) (INT) Okuda, Y.; Sasaki, D.; Nogami, S.; Kaneko, Y.; Ohya, Y.; Anraku, Y. (2003) Occurrence, horizontal transfer and degeneration of VDE intein family in Saccharomycete yeasts. Yeast 20(7): 563-73. PubMed ID: 12734795
853.  (APP) (EPL) (Structure) (TAG) Olsen, S. K.; Capili, A. D.; Lu, X.; Tan, D. S.; Lima, C. D. (2010) Active site remodelling accompanies thioester bond formation in the SUMO E1. Nature 463(7283): 906-12. PubMed ID: 20164921
854.  (INT) Omura, S.; Ikeda, H.; Ishikawa, J.; Hanamoto, A.; Takahashi, C.; Shinose, M.; Takahashi, Y.; Horikawa, H.; Nakazawa, H.; Osonoe, T.; Kikuchi, H.; Shiba, T.; Sakaki, Y.; Hattori, M. (2001) Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites. Proc Natl Acad Sci U S A. 98(21): 12215-20. Epub 2001 Sep 25.. PubMed ID: 11572948
855.  (APP) (PUR) Osicka, R.; Prochazkova, K.; Sulc, M.; Linhartova, I.; Havlicek, V.; Sebo, P. (2004) A novel "clip-and-link" activity of repeat in toxin (RTX) proteins from gram-negative pathogens. Covalent protein cross-linking by an Asp-Lys isopeptide bond upon calcium-dependent processing at an Asp-Pro bond. J Biol Chem 279(24): 24944-56. PubMed ID: 15044436
856.  (REL) Ostermeier, M. (2005) Engineering allosteric protein switches by domain insertion. Protein Eng Des Sel 18(8): 359-64. PubMed ID: 16043448
857.  (APP) (NMR) (Structure) (TS) Otomo, T.; Teruya, K.; Uegaki, K.; Yamazaki, T.; Kyogoku, Y. (1999) Improved segmental isotope labeling of proteins and application to a larger protein. J Biomol NMR 14(2): 105-14. PubMed ID: 10427740
858.  (APP) (NMR) (Structure) (TS) Otomo, T.; Ito, N.; Kyogoku, Y.; Yamazaki, T. (1999B) NMR observation of selected segments in a larger protein: central-segment isotope labeling through intein-mediated ligation. Biochemistry 38(49): 16040-4. PubMed ID: 10587426
859.  (APP) (EPL) Ottesen, J. J.; Huse, M.; Sekedat, M. D.; Muir, T. W. (2004) Semisynthesis of phosphovariants of Smad2 reveals a substrate preference of the activated T beta RI kinase. Biochemistry 43(19): 5698-706. PubMed ID: 15134444
860.  (APP) (EPL) (TAG) Ottesen, J. J.; Bar-Dagan, M.; Giovani, B.; Muir, T. W. (2008) An amalgamation of solid phase peptide synthesis and ribosomal peptide synthesis. Biopolymers 90(3): 406-14. PubMed ID: 17636509
861.  (APP) (EPL) Ovaa, H.; Galardy, P. J.; Ploegh, H. L. (2005) Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: synthesis of active site-directed probes. Methods Enzymol 399: 468-78. PubMed ID: 16338376
862.  (APP) (TAG) (TS) Ozawa, T.; Nogami, S.; Sato, M.; Ohya, Y.; Umezawa, Y. (2000) A fluorescent indicator for detecting protein-protein interactions in vivo based on protein splicing. Anal Chem 72(21): 5151-7. PubMed ID: 11080857
863.  (APP) (TAG) (TS) Ozawa, T.; Kaihara, A.; Sato, M.; Tachihara, K.; Umezawa, Y. (2001) Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing. Anal Chem 73(11): 2516-21. PubMed ID: 11403293
864.  (APP) (REV) (TS) Ozawa, T.; Umezawa, Y. (2001B) Detection of protein-protein interactions in vivo based on protein splicing. Curr Opin Chem Biol 5(5): 578-83. PubMed ID: 11578933
865.  (APP) (TAG) (TS) Ozawa, T.; Takeuchi, T. M.; Kaihara, A.; Sato, M.; Umezawa, Y. (2001C) Protein splicing-based reconstitution of split green fluorescent protein for monitoring protein-protein interactions in bacteria: improved sensitivity and reduced screening time. Anal Chem 73(24): 5866-74. PubMed ID: 11791555
866.  (APP) (PUR) (TAG) (TS) Ozawa, T.; Sako, Y.; Sato, M.; Kitamura, T.; Umezawa, Y. (2003) A genetic approach to identifying mitochondrial proteins. Nat Biotechnol 21(3): 287-93. PubMed ID: 12577068
867.  (APP) (TAG) (TS) Ozawa, T.; Nishitani, K.; Sako, Y.; Umezawa, Y. (2005) A high-throughput screening of genes that encode proteins transported into the endoplasmic reticulum in mammalian cells. Nucleic Acids Res 33(4): e34. PubMed ID: 15731327
868.  (APP) (REV) (TS) Ozawa, T.; Umezawa, Y. (2005B) Inteins for Split-Protein Reconstitutions and Their Applications. In: Homing Endonucleases and Inteins, ed. Belfort, M.; Derbyshire, V.; Stoddard, B.L.; Wood, D.W.. (Springer-Verlag, Heidelberg, Germany) Vol. 16 pages 307-324.
869.  (APP) (REV) (TAG) (TS) Ozawa, T. (2005D) Methods of analysis for protein dynamics in living cells based on protein splicing. Bulletin of the Chemical Society of Japan 78(5): 739-751.
870.  (APP) (REV) (TAG) (TS) Ozawa, T. (2006) Designing split reporter proteins for analytical tools. Anal Chim Acta 556(1): 58-68. PubMed ID: 17723331
871.  (APP) (PUR) (TS) Ozawa, T.; Umezawa, Y. (2007) Identification of proteins targeted into the endoplasmic reticulum by cDNA library screening. Methods Mol Biol 390: 269-80. PubMed ID: 17951694
872.  (APP) (TS) Ozawa, T.; Natori, Y.; Sako, Y.; Kuroiwa, H.; Kuroiwa, T.; Umezawa, Y. (2007) A minimal peptide sequence that targets fluorescent and functional proteins into the mitochondrial intermembrane space. ACS Chem Biol 2(3): 176-86. PubMed ID: 17348629
873.  (APP) (PUR) (TS) Ozawa, T.; Umezawa, Y. (2007B) A genetic method to identify mitochondrial proteins in living Mammalian cells. Methods Mol Biol 390: 119-30. PubMed ID: 17951684
874.  (APP) (TAG) (TS) Ozawa, T. (2009) Protein reconstitution methods for visualizing biomolecular function in living cells. Yakugaku Zasshi 129(3): 289-295.
875.  (APP) (EPL) (REL) Pal, G.; Santamaria, F.; Kossiakoff, A. A.; Lu, W. (2003) The first semi-synthetic serine protease made by native chemical ligation. Protein Expr Purif. 29(2): 185-92.. PubMed ID: 12767808
876.  (APP) (EPL) (REV) Panigrahi, K.; Eggen, M.; Maeng, J. H.; Shen, Q.; Berkowitz, D. B. (2009) The alpha,alpha-difluorinated phosphonate L-pSer-analogue: an accessible chemical tool for studying kinase-dependent signal transduction. Chem Biol 16(9): 928-36. PubMed ID: 19778720
877.  (INT) Papavinasasundaram, K. G.; Colston, M. J.; Davis, E. O. (1998) Construction and complementation of a recA deletion mutant of Mycobacterium smegmatis reveals that the intein in Mycobacterium tuberculosis recA does not affect RecA function. Mol Microbiol 30(3): 525-34. PubMed ID: 9822818
878.  (PUR) Park, C. M.; Kim, J. I.; Yang, S. S.; Kang, J. G.; Kang, J. H.; Shim, J. Y.; Chung, Y. H.; Park, Y. M.; Song, P. S. (2000) A second photochromic bacteriophytochrome from Synechocystis sp. PCC 6803: spectral analysis and down-regulation by light. Biochemistry 39(35): 10840-7. PubMed ID: 10978170
879.  (APP) (PUR) Park, S. J.; Seo, M. D.; Lee, S. K.; Ikeda, M.; Longnecker, R.; Lee, B. J. (2005) Expression and characterization of N-terminal domain of Epstein-Barr virus latent membrane protein 2A in Escherichia coli. Protein Expr Purif 41(1): 9-17. PubMed ID: 15802216
880.  (APP) (CPS) (PUR) (REV) Pastuszka, M. K.; Mackay, J. A. (2010) Biomolecular engineering of intracellular switches in eukaryotes. Journal of Drug Delivery Science and Technology 20(3): 163-169.
881.  (APP) (PUR) Paul, R.; Bosch, F. U.; Schafer, K. P. (2001) Overexpression and purification of Helicobacter pylori flavodoxin and induction of a specific antiserum in rabbits. Protein Expr Purif 22(3): 399-405. PubMed ID: 11483001
882.  (INT) Paul, J. H.; Williamson, S. J.; Long, A.; Authement, R. N.; John, D.; Segall, A. M.; Rohwer, F. L.; Androlewicz, M.; Patterson, S. (2005) Complete genome sequence of phiHSIC, a pseudotemperate marine phage of Listonella pelagia. Appl Environ Microbiol 71(6): 3311-20. PubMed ID: 15933034
883.  (APP) (EPL) (TAG) Paulick, M. G.; Wise, A. R.; Forstner, M. B.; Groves, J. T.; Bertozzi, C. R. (2007) Synthetic analogues of glycosylphosphatidylinositol-anchored proteins and their behavior in supported lipid bilayers. J Am Chem Soc 129(37): 11543-50. PubMed ID: 17715922
884.  (APP) (EPL) (TAG) Paulick, M. G.; Forstner, M. B.; Groves, J. T.; Bertozzi, C. R. (2007) A chemical approach to unraveling the biological function of the glycosylphosphatidylinositol anchor. Proc Natl Acad Sci U S A 104(51): 20332-7. PubMed ID: 18077333
885.  (APP) (CPS) (TAG) (TS) Paulmurugan, R.; Umezawa, Y.; Gambhir, S. S. (2002) Noninvasive imaging of protein-protein interactions in living subjects by using reporter protein complementation and reconstitution strategies. Proc Natl Acad Sci U S A 99(24): 15608-13. PubMed ID: 12438689
886.  (INT) Paulsen, I. T.; Press, C. M.; Ravel, J.; Kobayashi, D. Y.; Myers, G. S.; Mavrodi, D. V.; DeBoy, R. T.; Seshadri, R.; Ren, Q.; Madupu, R.; Dodson, R. J.; Durkin, A. S.; Brinkac, L. M.; Daugherty, S. C.; Sullivan, S. A.; Rosovitz, M. J.; Gwinn, M. L.; Zhou, L.; Schneider, D. J.; Cartinhour, S. W.; Nelson, W. C.; Weidman, J.; Watkins, K.; Tran, K.; Khouri, H.; Pierson, E. A.; Pierson, L. S., 3rd; Thomashow, L. S.; Loper, J. E. (2005) Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5. Nat Biotechnol. 23(7): 873-8. Epub 2005 Jun 26.. PubMed ID: 15980861
887.  (INT) (REV) Paulus, H. (1998) Protein splicing: A novel form of gene expression and paradigm for self-catalyzed protein rearrangements. Pure & Appl. Chem. 70: 1-8.
888.  (INT) (REV) Paulus, H. (1998B) The chemical basis of protein splicing. Chemical Society Reviews 27: 375-386.
889.  (INT) (REV) Paulus, H. (2000) Protein splicing and related forms of protein autoprocessing. Annu Rev Biochem 69: 447-96. PubMed ID: 10966466
890.  (INT) (REV) Paulus, H. (2001) Inteins as enzymes. Bioorg Chem 29(3): 119-29. PubMed ID: 11437387
891.  (APP) (REV) Paulus, H. (2003) Inteins as targets for potential antimycobacterial drugs. Front Biosci 8: s1157-65. PubMed ID: 12957838
892.  (APP) (CPS) Paulus, H. (2007) Protein splicing inhibitors as a new class of antimycobacterial agents. Drugs of the Future 32(11): 973-984.
893.  (INT) Pearl, E. J.; Tyndall, J. D.; Poulter, R. T.; Wilbanks, S. M. (2007) Sequence requirements for splicing by the Cne PRP8 intein. FEBS Lett 581(16): 3000-4. PubMed ID: 17544410
894.  (INT) Pearl, E. J.; Bokor, A. A.; Butler, M. I.; Poulter, R. T.; Wilbanks, S. M. (2007B) Preceding hydrophobic and beta-branched amino acids attenuate splicing by the CnePRP8 intein. Biochim Biophys Acta 1774(8): 995-1001. PubMed ID: 17604706
895.  (APP) (REL) (TAG) Pechlivanis, M.; Kuhlmann, J. (2006) Hydrophobic modifications of Ras proteins by isoprenoid groups and fatty acids--More than just membrane anchoring. Biochim Biophys Acta 1764(12): 1914-31. PubMed ID: 17110180
896.  (INT) Pedulla, M. L.; Ford, M. E.; Houtz, J. M.; Karthikeyan, T.; Wadsworth, C.; Lewis, J. A.; Jacobs-Sera, D.; Falbo, J.; Gross, J.; Pannunzio, N. R.; Brucker, W.; Kumar, V.; Kandasamy, J.; Keenan, L.; Bardarov, S.; Kriakov, J.; Lawrence, J. G.; Jacobs, W. R., Jr.; Hendrix, R. W.; Hatfull, G. F. (2003) Origins of highly mosaic mycobacteriophage genomes. Cell. 113(2): 171-82.. PubMed ID: 12705866
897.  (APP) (REL) (REV) Pelletier, J.; Sidhu, S. (2001) Mapping protein-protein interactions with combinatorial biology methods. Curr Opin Biotechnol. 12(4): 340-7.. PubMed ID: 11551461
898.  (APP) (EPL) (TAG) Pellois, J. P.; Hahn, M. E.; Muir, T. W. (2004) Simultaneous triggering of protein activity and fluorescence. J Am Chem Soc. 126(23): 7170-1.. PubMed ID: 15186142
899.  (APP) Pellois, J. P.; Muir, T. W. (2005) A ligation and photorelease strategy for the temporal and spatial control of protein function in living cells. Angew Chem Int Ed Engl 44(35): 5713-7. PubMed ID: 16059958
900.  (APP) (EPL) (REV) Pellois, J. P.; Muir, T. W. (2006) Semisynthetic proteins in mechanistic studies: using chemistry to go where nature can't. Curr Opin Chem Biol 10(5): 487-91. PubMed ID: 16935551
901.  (APP) (REL) Pentelute, B. L.; Kent, S. B. (2007) Selective desulfurization of cysteine in the presence of Cys(Acm) in polypeptides obtained by native chemical ligation. Org Lett 9(4): 687-90. PubMed ID: 17286375
902.  (EN) (INT) (NEB) Perler, F. B.; Comb, D. G.; Jack, W. E.; Moran, L. S.; Qiang, B.; Kucera, R. B.; Benner, J.; Slatko, B. E.; Nwankwo, D. O.; Hempstead, S. K.; et al., (1992) Intervening sequences in an Archaea DNA polymerase gene. Proc Natl Acad Sci U S A. 89(12): 5577-81.. PubMed ID: 1608969
903.  (INT) (NEB) Perler, F. B.; Davis, E. O.; Dean, G. E.; Gimble, F. S.; Jack, W. E.; Neff, N.; Noren, C. J.; Thorner, J.; Belfort, M. (1994) Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature. Nucleic Acids Res 22(7): 1125-7. PubMed ID: 8165123
904.  (INT) (NEB) (REV) Perler, F. B.; Kumar, S.; Kong, H. (1996) Thermostable DNA polymerases. Adv Protein Chem 48: 377-435. PubMed ID: 8791630
905.  (INT) (NEB) Perler, F. B.; Olsen, G. J.; Adam, E. (1997) Compilation and analysis of intein sequences. Nucleic Acids Res 25(6): 1087-93. PubMed ID: 9092614
906.  (APP) (NEB) (REV) Perler, F. B.; Xu, M. Q.; Paulus, H. (1997B) Protein splicing and autoproteolysis mechanisms. Curr Opin Chem Biol 1(3): 292-9. PubMed ID: 9667864
907.  (INT) (NEB) (REV) (Structure) Perler, F. B. (1998) Protein splicing of inteins and hedgehog autoproteolysis: structure, function, and evolution. Cell 92(1): 1-4. PubMed ID: 9489693
908.  (NEB) (REL) (REV) Perler, F. B. (1998B) Breaking up is easy with esters. Nat Struct Biol 5(4): 249-52. PubMed ID: 9546209
909.  (INT) (NEB) Perler, F. B. (1998C) InBase: The NEB Intein Database. The NEB Transcript 9(1): 12.
910.  (INT) (NEB) Perler, F. B. (1999) InBase, the New England Biolabs Intein Database. Nucleic Acids Res 27(1): 346-7. PubMed ID: 9847224
911.  (NEB) (REV) (TS) Perler, F. B. (1999B) A natural example of protein trans-splicing. Trends Biochem Sci 24(6): 209-11. PubMed ID: 10366843
912.  (INT) (NEB) (REV) Perler, F. B.; Xu, M.; Noren, C. J. (1999C) Protein Splicing. In: The Encyclopedia of Molecular Biology, ed. Creighton, T E. (John Wiley and Sons, New York) Vol. 3 pages 2011-2016.
913.  (INT) (NEB) Perler, F. B. (2000) InBase, the Intein Database. Nucleic Acids Res 28(1): 344-5. PubMed ID: 10592269
914.  (APP) (NEB) (REV) Perler, F. B.; Adam, E. (2000B) Protein splicing and its applications. Curr Opin Biotechnol 11(4): 377-83. PubMed ID: 10975457
915.  (INT) (NEB) (REV) Perler, F. B. (2001) Hyperthermophilic inteins. Methods Enzymol 334: 270-80. PubMed ID: 11398469
916.  (INT) (NEB) (REV) Perler, F. B. (2002) InBase: the Intein Database. Nucleic Acids Res 30(1): 383-4. PubMed ID: 11752343
917.  (INT) (NEB) (REV) Perler, Francine B. ; Chen, Lixin; Southworth, Maurice W. (2002B) The mechanism of protein splicing: variations on a theme. In: Peptides 2002: Proceedings of the Twenty-Seventh European Peptide Symposium, ed. Benedetti, E. ; Pedone, C.. (Edizioni Ziino, Napoli, Italy) pages 254-255.
918.  (CPS) (NEB) (REV) Perler, F. B. (2004) The ins and outs of gene expression control. Nat Biotechnol 22(7): 824-6. PubMed ID: 15229542
919.  (APP) (NEB) (REV) Perler, F. B. (2005) Protein splicing mechanisms and applications. IUBMB Life 57(7): 469-76. PubMed ID: 16081367
920.  (INT) (NEB) (REV) Perler, F.B. (2005B) Inteins - a historic perspective. In: Homing Endonucleases and Inteins, ed. Belfort, M.; Derbyshire, V.; Stoddard, B.L.; Wood, D.W.. (Springer-Verlag, Heidelberg, Germany) Vol. 16 pages 193-210.
921.  (APP) (INT) (NEB) (REV) Perler, F. B. (2006) Protein splicing mechanisms and applications. IUBMB Life 58(1): 63. PubMed ID: 16540435
922.  (APP) (CPS) (NEB) (REV) (TS) Perler, F. B. (2007) Shining a light on protein expression in living organisms. Nat Chem Biol 3(1): 17-8. PubMed ID: 17173022
923.  (APP) (INT) (NEB) (REV) Perler, F. B.; Southworth, M. W. ; Brace, L. (2007B) Inteins in Action. Proceedings of International Symposium on Extremophiles and Their Applications (http://www.jstage.jst.go.jp/browse/isea) 2005: 124-130.
924.  (APP) (PUR) Peroza, E. A.; Freisinger, E. (2008) Tris is a non-innocent buffer during intein-mediated protein cleavage. Protein Expr Purif 57(2): 217-25. PubMed ID: 18032065
925.  (APP) (REV) Peters, R.; Sikorski, R. (1998) Chemical weddings. Science. 281(5375): 367-8.. PubMed ID: 9705714
926.  (APP) (PUR) Pezza, J. A.; Allen, K. N.; Tolan, D. R. (2004) Intein-mediated purification of a recombinantly expressed peptide. Chem Commun (Camb) (21): 2412-3. PubMed ID: 15514791
927.  (INT) Pfeiffer, F.; Schuster, S. C.; Broicher, A.; Falb, M.; Palm, P.; Rodewald, K.; Ruepp, A.; Soppa, J.; Tittor, J.; Oesterhelt, D. (2008) Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1. Genomics 91(4): 335-46. PubMed ID: 18313895
928.  (APP) (EPL) Pickin, K. A.; Chaudhury, S.; Dancy, B. C.; Gray, J. J.; Cole, P. A. (2008) Analysis of protein kinase autophosphorylation using expressed protein ligation and computational modeling. J Am Chem Soc 130(17): 5667-9. PubMed ID: 18396877
929.  (EN) (INT) Pietrokovski, S. (1994) Conserved sequence features of inteins (protein introns) and their use in identifying new inteins and related proteins. Protein Sci 3(12): 2340-50. PubMed ID: 7756989
930.  (INT) Pietrokovski, S. (1996) A new intein in cyanobacteria and its significance for the spread of inteins. Trends Genet 12(8): 287-8. PubMed ID: 8783935
931.  (EN) (INT) Pietrokovski, S. (1998) Modular organization of inteins and C-terminal autocatalytic domains. Protein Sci 7(1): 64-71. PubMed ID: 9514260
932.  (INT) Pietrokovski, S. (1998B) Identification of a virus intein and a possible variation in the protein-splicing reaction. Curr Biol 8(18): R634-5. PubMed ID: 9740808
933.  (INT) (REV) Pietrokovski, S. (2001) Intein spread and extinction in evolution. Trends Genet 17(8): 465-72. PubMed ID: 11485819
934.  (INT) Pikuta, E. V.; Marsic, D.; Itoh, T.; Bej, A. K.; Tang, J.; Whitman, W. B.; Ng, J. D.; Garriott, O. K.; Hoover, R. B. (2007) Thermococcus thioreducens sp. nov., a novel hyperthermophilic, obligately sulfur-reducing archaeon from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 57(Pt 7): 1612-8. PubMed ID: 17625204
935.  (EN) (INT) Pingoud, V.; Grindl, W.; Wende, W.; Thole, H.; Pingoud, A. (1998) Structural and functional analysis of the homing endonuclease PI-SceI by limited proteolytic cleavage and molecular cloning of partial digestion products. Biochemistry 37(22): 8233-43. PubMed ID: 9609720
936.  (EN) (INT) Pingoud, V.; Thole, H.; Christ, F.; Grindl, W.; Wende, W.; Pingoud, A. (1999) Photocross-linking of the homing endonuclease PI-SceI to its recognition sequence. J Biol Chem 274(15): 10235-43. PubMed ID: 10187809
937.  (APP) (PUR) Pinto, A. P.; Campana, P. T.; Beltramini, L. M.; Silber, A. M.; Araujo, A. P. (2003) Structural characterization of a recombinant flagellar calcium-binding protein from Trypanosoma cruzi. Biochim Biophys Acta 1652(2): 107-14. PubMed ID: 14644046
938.  (APP) (EPL) Piontek, C.; Ring, P.; Harjes, O.; Heinlein, C.; Mezzato, S.; Lombana, N.; Pohner, C.; Puttner, M.; Varon Silva, D.; Martin, A.; Schmid, F. X.; Unverzagt, C. (2009) Semisynthesis of a homogeneous glycoprotein enzyme: ribonuclease C: part 1. Angew Chem Int Ed Engl 48(11): 1936-40. PubMed ID: 19173366
939.  (APP) (EPL) Piontek, C.; Varon Silva, D.; Heinlein, C.; Pohner, C.; Mezzato, S.; Ring, P.; Martin, A.; Schmid, F. X.; Unverzagt, C. (2009B) Semisynthesis of a homogeneous glycoprotein enzyme: ribonuclease C: part 2. Angew Chem Int Ed Engl 48(11): 1941-5. PubMed ID: 19180621
940.  (APP) (EPL) (PUR) (REV) Platis, D.; Labrou, N. E. (2008) Chemical and genetic engineering strategies to improve the potency of pharmaceutical proteins and enzymes. Current Medicinal Chemistry 15(19): 1940-1955.
941.  (EN) (INT) (Structure) Poland, B. W.; Xu, M. Q.; Quiocho, F. A. (2000) Structural insights into the protein splicing mechanism of PI-SceI. J Biol Chem 275(22): 16408-13. PubMed ID: 10828056
942.  (APP) (NEB) (PUR) Porte, M.; Chong, S. (2008) Single-process expression and purification of multiple recombinant proteins through cocultivation and affinity purification. Anal Biochem 381(1): 175-7. PubMed ID: 18602361
943.  (REL) Porter, J. A.; von Kessler, D. P.; Ekker, S. C.; Young, K. E.; Lee, J. J.; Moses, K.; Beachy, P. A. (1995) The product of hedgehog autoproteolytic cleavage active in local and long-range signalling. Nature 374(6520): 363-6. PubMed ID: 7885476
944.  (REL) Porter, J. A.; Ekker, S. C.; Park, W. J.; von Kessler, D. P.; Young, K. E.; Chen, C. H.; Ma, Y.; Woods, A. S.; Cotter, R. J.; Koonin, E. V.; Beachy, P. A. (1996) Hedgehog patterning activity: role of a lipophilic modification mediated by the carboxy-terminal autoprocessing domain. Cell 86(1): 21-34. PubMed ID: 8689684
945.  (EN) (INT) Posey, K. L.; Gimble, F. S. (2002) Insertion of a reversible redox switch into a rare-cutting DNA endonuclease. Biochemistry 41(7): 2184-90. PubMed ID: 11841209
946.  (EN) (INT) Posey, K. L.; Koufopanou, V.; Burt, A.; Gimble, F. S. (2004) Evolution of divergent DNA recognition specificities in VDE homing endonucleases from two yeast species. Nucleic Acids Res 32(13): 3947-56. PubMed ID: 15280510
947.  (INT) (REV) Poulter, R. T.; Goodwin, T. J.; Butler, M. I. (2007) The nuclear-encoded inteins of fungi. Fungal Genet Biol 44(3): 153-79. PubMed ID: 17046294
948.  (INT) Powers, Taryn L.; Drago, Matthew J.; Dorval, Deirdre M.; Connor, Katherine R.; Mills, Kenneth V. (2006) Protein splicing of a non-canonical Clostridium thermocellum intein with N-terminal Gln. FASEB J._MeetingAbstracts 20(5): A964-b.
949.  (APP) (NEB) (PUR) Pradhan, S.; Bacolla, A.; Wells, R. D.; Roberts, R. J. (1999) Recombinant human DNA (cytosine-5) methyltransferase. I. Expression, purification, and comparison of de novo and maintenance methylation. J Biol Chem 274(46): 33002-10. PubMed ID: 10551868
950.  (APP) (REL) Pratt, M. R.; Schwartz, E. C.; Muir, T. W. (2007) Small-molecule-mediated rescue of protein function by an inducible proteolytic shunt. Proc Natl Acad Sci U S A 104(27): 11209-14. PubMed ID: 17563385
951.  (APP) (PUR) (REV) (TAG) Prinz, A.; Reither, G.; Diskar, M.; Schultz, C. (2008) Fluorescence and bioluminescence procedures for functional proteomics. Proteomics 8(6): 1179-96. PubMed ID: 18283669
952.  (APP) (PUR) Prochazkova, K.; Osicka, R.; Linhartova, I.; Halada, P.; Sulc, M.; Sebo, P. (2005) The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC. J Biol Chem 280(5): 3251-8. PubMed ID: 15525636
953.  (APP) (PUR) Qi, Z.; Hamza, I.; O'Brian, M. R. (1999) Heme is an effector molecule for iron-dependent degradation of the bacterial iron response regulator (Irr) protein. Proc Natl Acad Sci U S A 96(23): 13056-61. PubMed ID: 10557272
954.  (APP) (REV) Qi, D.; Tann, C. M.; Haring, D.; Distefano, M. D. (2001) Generation of new enzymes via covalent modification of existing proteins. Chem Rev. 101(10): 3081-111.. PubMed ID: 11710063
955.  (NEB) (REL) Qian, X.; Guan, C.; Guo, H. C. (2003) A dual role for an aspartic acid in glycosylasparaginase autoproteolysis. Structure 11(8): 997-1003. PubMed ID: 12906830
956.  (REL) Radkiewicz, J. L.; Zipse, H.; Clarke, S.; Houk, K. N. (2001) Neighboring side chain effects on asparaginyl and aspartyl degradation: an ab initio study of the relationship between peptide conformation and backbone NH acidity. J Am Chem Soc 123(15): 3499-506. PubMed ID: 11472122
957.  (INT) Raghavan, R.; Hicks, L. D.; Minnick, M. F. (2008) Toxic introns and parasitic intein in Coxiella burnetii: legacies of a promiscuous past. J Bacteriol 190(17): 5934-43. PubMed ID: 18606739
958.  (EN) (INT) (REV) Raghavan, R.; Minnick, M. F. (2009) Group I introns and inteins: disparate origins but convergent parasitic strategies. J Bacteriol 191(20): 6193-202. PubMed ID: 19666710
959.  (APP) (PUR) Raibekas, A. A.; Fukui, K.; Massey, V. (2000) Design and properties of human D-amino acid oxidase with covalently attached flavin. Proc Natl Acad Sci U S A 97(7): 3089-93. PubMed ID: 10716694
960.  (APP) (PUR) (Structure) Rak, A.; Pylypenko, O.; Durek, T.; Watzke, A.; Kushnir, S.; Brunsveld, L.; Waldmann, H.; Goody, R. S.; Alexandrov, K. (2003) Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase. Science 302(5645): 646-50. PubMed ID: 14576435
961.  (APP) (EPL) (REL) (Structure) Rak, A.; Pylypenko, O.; Niculae, A.; Pyatkov, K.; Goody, R. S.; Alexandrov, K. (2004) Structure of the Rab7:REP-1 complex: insights into the mechanism of Rab prenylation and choroideremia disease. Cell. 117(6): 749-60.. PubMed ID: 15186776
962.  (APP) (EPL) Ralle, M.; Berry, S. M.; Nilges, M. J.; Gieselman, M. D.; van der Donk, W. A.; Lu, Y.; Blackburn, N. J. (2004) The selenocysteine-substituted blue copper center: spectroscopic investigations of Cys112SeCys Pseudomonas aeruginosa azurin. J Am Chem Soc 126(23): 7244-56. PubMed ID: 15186162
963.  (REL) (REV) Rammensee, H. G. (2004) Immunology: protein surgery. Nature 427(6971): 203-4. PubMed ID: 14724617
964.  (INT) Raoult, D.; Audic, S.; Robert, C.; Abergel, C.; Renesto, P.; Ogata, H.; La Scola, B.; Suzan, M.; Claverie, J. M. (2004) The 1.2-megabase genome sequence of Mimivirus. Science 306(5700): 1344-50. PubMed ID: 15486256
965.  (APP) (REV) Rauh, D.; Waldmann, H. (2007) Linking chemistry and biology for the study of protein function. Angew Chem Int Ed Engl 46(6): 826-9. PubMed ID: 17195266
966.  (APP) (PUR) Rebets, Y.; Ostash, B.; Luzhetskyy, A.; Kushnir, S.; Fukuhara, M.; Bechthold, A.; Nashimoto, M.; Nakamura, T.; Fedorenko, V. (2005) DNA-binding activity of LndI protein and temporal expression of the gene that upregulates landomycin E production in Streptomyces globisporus 1912. Microbiology 151(Pt 1): 281-90. PubMed ID: 15632445
967.  (INT) Reith, M.E.; Munholland, J. (1995) Complete nucleotide seuence of the ; Porphyra purpurea; chloroplast genome.. Plant Mol. Biol. Rep. 13: 333-335.
968.  (APP) (CYCL) (NMR) Reitinger, S.; Yu, Y.; Wicki, J.; Ludwiczek, M.; D'Angelo, I.; Baturin, S.; Okon, M.; Strynadka, N. C.; Lutz, S.; Withers, S. G.; McIntosh, L. P. (2010) Circular permutation of Bacillus circulans xylanase: a kinetic and structural study. Biochemistry 49(11): 2464-74. PubMed ID: 20163191
969.  (INT) Reitter, Julie; Schufreider, Ann K.; Mills, Kenneth V. (2009) Protein Splicing by a Non-Canonical Mechanism. FASEB J._MeetingAbstracts 23(1_MeetingAbstracts): 677.3.
970.  (INT) Rementeria, A.; Lopez-Molina, N.; Ludwig, A.; Vivanco, A. B.; Bikandi, J.; Ponton, J.; Garaizar, J. (2005) Genes and molecules involved in Aspergillus fumigatus virulence. Revista Iberoamericana de Micologia 22(1): 1-23.
971.  (APP) (REL) Reulen, S. W.; Brusselaars, W. W.; Langereis, S.; Mulder, W. J.; Breurken, M.; Merkx, M. (2007) Protein-liposome conjugates using cysteine-lipids and native chemical ligation. Bioconjug Chem 18(2): 590-6. PubMed ID: 17315942
972.  (APP) (PUR) (TAG) Reulen, S. W.; van Baal, I.; Raats, J. M.; Merkx, M. (2009) Efficient, chemoselective synthesis of immunomicelles using single-domain antibodies with a C-terminal thioester. BMC Biotechnol 9: 66. PubMed ID: 19619333
973.  (APP) (EPL) (PUR) (TAG) Richter, Michael P. O.; Holland-Nell, Kai; Beck-Sickinger, Annette G. (2004) Site specific biotinylation of the human aldo/keto reductase AKR1A1 for immobilization. Tetrahedron 60(35): 7507-7513.
974.  (APP) (EPL) Richter, M. P.; Beck-Sickinger, A. G. (2005) Expressed protein ligation to obtain selectively modified aldo/keto reductases. Protein Pept Lett 12(8): 777-81. PubMed ID: 16305548
975.  (INT) (REV) Riddihough, G. (1997) Nice splice. Nat Struct Biol 4(7): 507-8. PubMed ID: 9228936
976.  (INT) Riera, J.; Robb, F. T.; Weiss, R.; Fontecave, M. (1997) Ribonucleotide reductase in the archaeon Pyrococcus furiosus: a critical enzyme in the evolution of DNA genomes?. Proc Natl Acad Sci U S A 94(2): 475-8. PubMed ID: 9012808
977.  (EN) (REV) Roberts, R. J.; Belfort, M.; Bestor, T.; Bhagwat, A. S.; Bickle, T. A.; Bitinaite, J.; Blumenthal, R. M.; Degtyarev, SKh; Dryden, D. T.; Dybvig, K.; Firman, K.; Gromova, E. S.; Gumport, R. I.; Halford, S. E.; Hattman, S.; Heitman, J.; Hornby, D. P.; Janulaitis, A.; Jeltsch, A.; Josephsen, J.; Kiss, A.; Klaenhammer, T. R.; Kobayashi, I.; Kong, H.; Kruger, D. H.; Lacks, S.; Marinus, M. G.; Miyahara, M.; Morgan, R. D.; Murray, N. E.; Nagaraja, V.; Piekarowicz, A.; Pingoud, A.; Raleigh, E.; Rao, D. N.; Reich, N.; Repin, V. E.; Selker, E. U.; Shaw, P. C.; Stein, D. C.; Stoddard, B. L.; Szybalski, W.; Trautner, T. A.; Van Etten, J. L.; Vitor, J. M.; Wilson, G. G.; Xu, S. Y. (2003) A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucleic Acids Res. 31(7): 1805-12.. PubMed ID: 12654995
978.  (EN) Roberts, R. J.; Vincze, T.; Posfai, J.; Macelis, D. (2005) REBASE--restriction enzymes and DNA methyltransferases. Nucleic Acids Res. 33(Database issue): D230-2.. PubMed ID: 15608184
979.  (APP) (REV) Robinson, C. V.; Cowburn, D. (2001) Analytical techniques: From individual proteins to whole cells. Current Opinion in Chemical Biology 5(5): 565-566.
980.  (APP) (REV) Roelfes, G.; Mootz, H. D. (2006) Probing the molecular basis of protein function through chemistry. Chembiochem 7(3): 545-9. PubMed ID: 16453350
981.  (INT) (REL) (REV) Rojas, A.; Garcia-VallveÃÅ, S.; Palau, J.; Romeu, A. (1999) Circular permutations in proteins. Biologia 54(3): 255-277.
982.  (APP) (EPL) (NMR) (PUR) (Structure) Romanelli, A.; Shekhtman, A.; Cowburn, D.; Muir, T. W. (2004) Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction. Proc Natl Acad Sci U S A 101(17): 6397-402. PubMed ID: 15087498
983.  (REL) Rosenblum, J. S.; Blobel, G. (1999) Autoproteolysis in nucleoporin biogenesis. Proc Natl Acad Sci U S A 96(20): 11370-5. PubMed ID: 10500183
984.  (APP) (EPL) Roy, R. S.; Allen, O.; Walsh, C. T. (1999) Expressed protein ligation to probe regiospecificity of heterocyclization in the peptide antibiotic microcin B17. Chem Biol 6(11): 789-99. PubMed ID: 10574779
985.  (INT) Ruepp, A.; Graml, W.; Santos-Martinez, M. L.; Koretke, K. K.; Volker, C.; Mewes, H. W.; Frishman, D.; Stocker, S.; Lupas, A. N.; Baumeister, W. (2000) The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum. Nature. 407(6803): 508-13.. PubMed ID: 11029001
986.  (REL) (REV) Ruvkun, G.; Hobert, O. (1998) The taxonomy of developmental control in Caenorhabditis elegans. Science 282(5396): 2033-41. PubMed ID: 9851920
987.  (INT) Ryan, C.; Stevens, T. H.; Schlesinger, M. J. (1992) Inhibitory effects of HSP70 chaperones on nascent polypeptides. Protein Sci. 1(8): 980-5.. PubMed ID: 1304386
988.  (INT) (REL) (REV) Saab-Rincón, G.; Valderrama, B. (2009) Protein engineering of redox-active enzymes. Antioxidants and Redox Signaling 11(2): 167-192.
989.  (APP) (PUR) (REL) Sadilkova, L.; Osicka, R.; Sulc, M.; Linhartova, I.; Novak, P.; Sebo, P. (2008) Single-step affinity purification of recombinant proteins using a self-excising module from Neisseria meningitidis FrpC. Protein Sci 17(10): 1834-43. PubMed ID: 18662906
990.  (APP) (PUR) Saiki, K.; Konishi, K.; Gomi, T.; Nishihara, T.; Yoshikawa, M. (2001) Reconstitution and purification of cytolethal distending toxin of Actinobacillus actinomycetemcomitans. Microbiol Immunol 45(6): 497-506. PubMed ID: 11497226
991.  (INT) Sakaguchi, Y.; Hayashi, T.; Kurokawa, K.; Nakayama, K.; Oshima, K.; Fujinaga, Y.; Ohnishi, M.; Ohtsubo, E.; Hattori, M.; Oguma, K. (2005) The genome sequence of Clostridium botulinum type C neurotoxin-converting phage and the molecular mechanisms of unstable lysogeny. Proc Natl Acad Sci U S A 102(48): 17472-7. PubMed ID: 16287978
992.  (INT) Sakakibara, N.; Han, M.; Rollor, C. R.; Gilson, R. C.; Busch, C.; Heo, G.; Kelman, Z. (2008) Cloning, Purification, and Partial Characterization of the Halobacterium sp. NRC-1 Minichromosome Maintenance (MCM) Helicase. Open Microbiol J 2: 13-7. PubMed ID: 19088906
993.  (APP) (PUR) Salazar, J. C.; Ahel, I.; Orellana, O.; Tumbula-Hansen, D.; Krieger, R.; Daniels, L.; Soll, D. (2003) Coevolution of an aminoacyl-tRNA synthetase with its tRNA substrates. Proc Natl Acad Sci U S A 100(24): 13863-8. PubMed ID: 14615592
994.  (APP) (INT) (NEB) (REV) Saleh, L.; Perler, F. B. (2006) Protein splicing in cis and in trans. Chem Rec 6(4): 183-93. PubMed ID: 16900466
995.  (APP) (CYCL) (REV) Sancheti, H.; Camarero, J. A. (2009) "Splicing up" drug discovery. Cell-based expression and screening of genetically-encoded libraries of backbone-cyclized polypeptides. Adv Drug Deliv Rev 61(11): 908-17. PubMed ID: 19628015
996.  (REL) Sandberg, A.; Johansson, D. G.; Macao, B.; Hard, T. (2008) SEA domain autoproteolysis accelerated by conformational strain: energetic aspects. J Mol Biol 377(4): 1117-29. PubMed ID: 18308334
997.  (INT) Sander, P.; Alcaide, F.; Richter, I.; Frischkorn, K.; Tortoli, E.; Springer, B.; Telenti, A.; Bottger, E. C. (1998) Inteins in mycobacterial GyrA are a taxonomic character. Microbiology 144 ( Pt 2): 589-91. PubMed ID: 9493395
998.  (INT) Sankararaman, S.; Sjolander, K. (2008) INTREPID--INformation-theoretic TREe traversal for Protein functional site IDentification. Bioinformatics 24(21): 2445-52. PubMed ID: 18776193
999.  (APP) (PUR) Sarkar, D.; Azaro, M. A.; Aihara, H.; Papagiannis, C. V.; Tirumalai, R.; Nunes-Duby, S. E.; Johnson, R. C.; Ellenberger, T.; Landy, A. (2002) Differential affinity and cooperativity functions of the amino-terminal 70 residues of lambda integrase. J Mol Biol 324(4): 775-89. PubMed ID: 12460577
1000.  (REL) (REV) Saska, I.; Craik, D. J. (2008) Protease-catalysed protein splicing: a new post-translational modification?. Trends Biochem Sci 33(8): 363-8. PubMed ID: 18599295
1001.  (EN) (INT) Saves, I.; Ozanne, V.; Dietrich, J.; Masson, J. M. (2000) Inteins of Thermococcus fumicolans DNA polymerase are endonucleases with distinct enzymatic behaviors. J Biol Chem 275(4): 2335-41. PubMed ID: 10644683
1002.  (INT) Saves, I.; Laneelle, M. A.; Daffe, M.; Masson, J. M. (2000B) Inteins invading mycobacterial RecA proteins. FEBS Lett 480(2-3): 221-5. PubMed ID: 11034333
1003.  (EN) (INT) Saves, I.; Eleaume, H.; Dietrich, J.; Masson, J. M. (2000C) The Thy pol-2 intein of Thermococcus hydrothermalis is an isoschizomer of PI-TliI and PI-TfuII endonucleases. Nucleic Acids Res 28(21): 4391-6. PubMed ID: 11058140
1004.  (EN) (INT) Saves, I.; Westrelin, F.; Daffe, M.; Masson, J. M. (2001) Identification of the first eubacterial endonuclease coded by an intein allele in the pps1 gene of mycobacteria. Nucleic Acids Res 29(21): 4310-8. PubMed ID: 11691918
1005.  (INT) Saves, I.; Daffe, M.; Masson, J. M. (2001B) Distribution of GyrA intein in non-tuberculous mycobacteria and genomic heterogeneity of Mycobacterium gastri. FEBS Lett 508(1): 121-5. PubMed ID: 11707281
1006.  (INT) Saves, I.; Lewis, L. A.; Westrelin, F.; Warren, R.; Daffe, M.; Masson, J. M. (2002) Specificities and functions of the recA and pps1 intein genes of Mycobacterium tuberculosis and application for diagnosis of tuberculosis. J Clin Microbiol 40(3): 943-50. PubMed ID: 11880421
1007.  (INT) Saves, I.; Daffe, M.; Masson, J. M. (2002) Erratum: Distribution of GyrA intein in non-tuberculous mycobacteria and genomic heterogeneity of Mycobacterium gastri (FEBS Letters (2001) 508 (121-125) PII: S001457930103037X). FEBS Letters 528(1-3): 284.
1008.  (EN) (INT) Saves, I.; Morlot, C.; Thion, L.; Rolland, J. L.; Dietrich, J.; Masson, J. M. (2002B) Investigating the endonuclease activity of four Pyrococcus abyssi inteins. Nucleic Acids Res 30(19): 4158-65. PubMed ID: 12364594
1009.  (APP) (INT) (REV) Sawczak, M.; Szweykowska-Kulinska, Z. (2004) [About inteins: their structure, evolution and applications]. Postepy Biochem 50(1): 69-81. PubMed ID: 15497645
1010.  (APP) (PUR) Schaffer, L.; Brissette, R. E.; Spetzler, J. C.; Pillutla, R. C.; Ostergaard, S.; Lennick, M.; Brandt, J.; Fletcher, P. W.; Danielsen, G. M.; Hsiao, K. C.; Andersen, A. S.; Dedova, O.; Ribel, U.; Hoeg-Jensen, T.; Hansen, P. H.; Blume, A. J.; Markussen, J.; Goldstein, N. I. (2003) Assembly of high-affinity insulin receptor agonists and antagonists from peptide building blocks. Proc Natl Acad Sci U S A. 100(8): 4435-9. Epub 2003 Apr 8.. PubMed ID: 12684539
1011.  (APP) (EPL) (TAG) Scheibner, K. A.; Zhang, Z.; Cole, P. A. (2003) Merging fluorescence resonance energy transfer and expressed protein ligation to analyze protein-protein interactions. Anal Biochem 317(2): 226-32. PubMed ID: 12758261
1012.  (APP) (PUR) Schleper, C.; Swanson, R. V.; Mathur, E. J.; DeLong, E. F. (1997) Characterization of a DNA polymerase from the uncultivated psychrophilic archaeon Cenarchaeum symbiosum. J Bacteriol 179(24): 7803-11. PubMed ID: 9401041
1013.  (APP) (PUR) Schlothauer, T.; Mogk, A.; Dougan, D. A.; Bukau, B.; Turgay, K. (2003) MecA, an adaptor protein necessary for ClpC chaperone activity. Proc Natl Acad Sci U S A 100(5): 2306-11. PubMed ID: 12598648
1014.  (APP) (PUR) Schmidt, U.; Rudolph, R.; Bohm, G. (2000) Mechanism of assembly of recombinant murine polyomavirus-like particles. J Virol 74(4): 1658-62. PubMed ID: 10644335
1015.  (EN) (INT) Schottler, S.; Wende, W.; Pingoud, V.; Pingoud, A. (2000) Identification of Asp218 and Asp326 as the principal Mg2+ binding ligands of the homing endonuclease PI-SceI. Biochemistry 39(51): 15895-900. PubMed ID: 11123916
1016.  (APP) (PUR) Schrader, N.; Kim, E. Y.; Winking, J.; Paulukat, J.; Schindelin, H.; Schwarz, G. (2004) Biochemical characterization of the high affinity binding between the glycine receptor and gephyrin. J Biol Chem 279(18): 18733-41. PubMed ID: 14976213
1017.  (INT) Schufreider, Ann K.; O'Brien, Kathryn M.; McGill, Melissa A.; Reitter, Julie N.; Mills, Kenneth V. (2009) Protein splicing of the Pyrococcus abyssi lon protease intein. FASEB J._MeetingAbstracts 23(1_MeetingAbstracts): 502.2.
1018.  (REL) Schuler, B.; Pannell, L. K. (2002) Specific labeling of polypeptides at amino-terminal cysteine residues using Cy5-benzyl thioester. Bioconjug Chem. 13(5): 1039-43. PubMed ID: 12236786
1019.  (APP) (EPL) Schumacher, M. C.; Resenberger, U.; Seidel, R. P.; Becker, C. F.; Winklhofer, K. F.; Oesterhelt, D.; Tatzelt, J.; Engelhard, M. (2010) Synthesis of a GPI anchor module suitable for protein post-translational modification. Biopolymers 94(4): 457-64. PubMed ID: 20593476
1020.  (APP) (INT) (REV) Schwartz, E. C.; Muir, T. W.; Tyszkiewicz, A. B. (2003) "The splice is right": how protein splicing is opening new doors in protein science. Chem Commun (Camb) (17): 2087-90. PubMed ID: 13678154
1021.  (APP) (CPS) (TS) Schwartz, E. C.; Saez, L.; Young, M. W.; Muir, T. W. (2007) Post-translational enzyme activation in an animal via optimized conditional protein splicing. Nat Chem Biol 3(1): 50-54. PubMed ID: 17128262
1022.  (APP) (PUR) Schwartz, E. C.; Shekhtman, A.; Dutta, K.; Pratt, M. R.; Cowburn, D.; Darst, S.; Muir, T. W. (2008) A full-length group 1 bacterial sigma factor adopts a compact structure incompatible with DNA binding. Chem Biol 15(10): 1091-103. PubMed ID: 18940669
1023.  (APP) (EPL) (REV) Schwarzer, D.; Cole, P. A. (2005) Protein semisynthesis and expressed protein ligation: chasing a protein's tail. Curr Opin Chem Biol 9(6): 561-9. PubMed ID: 16226484
1024.  (APP) (EPL) Schwarzer, D.; Zhang, Z.; Zheng, W.; Cole, P. A. (2006) Negative regulation of a protein tyrosine phosphatase by tyrosine phosphorylation. J Am Chem Soc 128(13): 4192-3. PubMed ID: 16568970
1025.  (APP) (CYCL) Scott, C. P.; Abel-Santos, E.; Wall, M.; Wahnon, D. C.; Benkovic, S. J. (1999) Production of cyclic peptides and proteins in vivo. Proc Natl Acad Sci U S A 96(24): 13638-43. PubMed ID: 10570125
1026.  (APP) (CYCL) Scott, C. P.; Abel-Santos, E.; Jones, A. D.; Benkovic, S. J. (2001) Structural requirements for the biosynthesis of backbone cyclic peptide libraries. Chem Biol 8(8): 801-15. PubMed ID: 11514229
1027.  (APP) (REV) Sears, P.; Tolbert, T.; Wong, C. H. (2001) Enzymatic approaches to glycoprotein synthesis. Genet Eng (N Y) 23: 45-68. PubMed ID: 11570106
1028.  (APP) (REV) Sears, P.; Wong, C. H. (2001B) Toward automated synthesis of oligosaccharides and glycoproteins. Science 291(5512): 2344-50. PubMed ID: 11269314
1029.  (INT) Senejani, A. G.; Hilario, E.; Gogarten, J. P. (2001) The intein of the Thermoplasma A-ATPase A subunit: structure, evolution and expression in E. coli. BMC Biochem 2: 13. PubMed ID: 11722801
1030.  (EN) (INT) (TAG) Senejani, A. G.; Gogarten, J. P. (2007) Structural stability and endonuclease activity of a PI-SceI GFP-fusion protein. Int J Biol Sci 3(4): 205-11. PubMed ID: 17389927
1031.  (INT) Seshadri, R.; Paulsen, I. T.; Eisen, J. A.; Read, T. D.; Nelson, K. E.; Nelson, W. C.; Ward, N. L.; Tettelin, H.; Davidsen, T. M.; Beanan, M. J.; Deboy, R. T.; Daugherty, S. C.; Brinkac, L. M.; Madupu, R.; Dodson, R. J.; Khouri, H. M.; Lee, K. H.; Carty, H. A.; Scanlan, D.; Heinzen, R. A.; Thompson, H. A.; Samuel, J. E.; Fraser, C. M.; Heidelberg, J. F. (2003) Complete genome sequence of the Q-fever pathogen Coxiella burnetii. Proc Natl Acad Sci U S A. 100(9): 5455-60. Epub 2003 Apr 18.. PubMed ID: 12704232
1032.  (APP) (EPL) Severinov, K.; Muir, T. W. (1998) Expressed protein ligation, a novel method for studying protein-protein interactions in transcription. J Biol Chem 273(26): 16205-9. PubMed ID: 9632677
1033.  (APP) (EPL) Seyedsayamdost, M. R.; Yee, C. S.; Reece, S. Y.; Nocera, D. G.; Stubbe, J. (2006) pH Rate profiles of FnY356-R2s (n = 2, 3, 4) in Escherichia coli ribonucleotide reductase: evidence that Y356 is a redox-active amino acid along the radical propagation pathway. J Am Chem Soc 128(5): 1562-8. PubMed ID: 16448127
1034.  (APP) (EPL) Seyedsayamdost, M. R.; Stubbe, J. (2006B) Site-Specific Replacement of Y(356) with 3,4-Dihydroxyphenylalanine in the beta2 Subunit of E. coli Ribonucleotide Reductase. J Am Chem Soc 128(8): 2522-3. PubMed ID: 16492021
1035.  (APP) (EPL) Seyedsayamdost, M. R.; Stubbe, J. (2007) Forward and Reverse Electron Transfer with the Y(356)DOPA-beta2 Heterodimer of E. coli Ribonucleotide Reductase. J Am Chem Soc 129(8): 2226-7. PubMed ID: 17279757
1036.  (APP) (EPL) (REV) (TAG) Seyedsayamdost, M. R.; Yee, C. S.; Stubbe, J. (2007C) Site-specific incorporation of fluorotyrosines into the R2 subunit of E. coli ribonucleotide reductase by expressed protein ligation. Nat Protoc 2(5): 1225-35. PubMed ID: 17546018
1037.  (APP) (CPS) Shah, R.V.; Finkelstein, J.M.; Verdine, G.L. (2002) Summer Research Report: Toward a Small-Molecule Activated Protein Splicing System. The Nucleus 80(7): 16.
1038.  (INT) (NEB) Shao, Y.; Xu, M. Q.; Paulus, H. (1995) Protein splicing: characterization of the aminosuccinimide residue at the carboxyl terminus of the excised intervening sequence. Biochemistry 34(34): 10844-50. PubMed ID: 7662664
1039.  (INT) (NEB) Shao, Y.; Xu, M. Q.; Paulus, H. (1996) Protein splicing: evidence for an N-O acyl rearrangement as the initial step in the splicing process. Biochemistry 35(12): 3810-5. PubMed ID: 8620003
1040.  (INT) (REV) Shao, Y.; Kent, S. B. (1997) Protein splicing: occurrence, mechanisms and related phenomena. Chem Biol 4(3): 187-94. PubMed ID: 9115411
1041.  (INT) Shao, Y.; Paulus, H. (1997B) Protein splicing: estimation of the rate of O-N and S-N acyl rearrangements, the last step of the splicing process. J Pept Res 50(3): 193-8. PubMed ID: 9309583
1042.  (APP) (PUR) Shao, X.; Gao, Y.; Jiang, M.; Li, L. (2009) Deletion and site-directed mutagenesis of laccase from Shigella dysenteriae results in enhanced enzymatic activity and thermostability. Enzyme and Microbial Technology 44(5): 274-280.
1043.  (APP) (INT) (NEB) (PUR) Sharma, S.; Zhang, A.; Wang, H.; Harcum, S. W.; Chong, S. (2003) Study of protein splicing and intein-mediated peptide bond cleavage under high-cell-density conditions. Biotechnol Prog 19(3): 1085-90. PubMed ID: 12790686
1044.  (APP) (NEB) (PUR) Sharma, S. S.; Chong, S.; Harcum, S. W. (2005) Simulation of large-scale production of a soluble recombinant protein expressed in Escherichia coli using an intein-mediated purification system. Appl Biochem Biotechnol 126(2): 93-118. PubMed ID: 16118465
1045.  (APP) (NEB) (PUR) Sharma, S. S.; Chong, S.; Harcum, S. W. (2006) Intein-mediated protein purification of fusion proteins expressed under high-cell density conditions in E. coli. J Biotechnol 125(1): 48-56. PubMed ID: 16546284
1046.  (REL) Shastri, N. (2006) Cell biology. Peptides, scrambled and stitched. Science 313(5792): 1398-9. PubMed ID: 16959995
1047.  (APP) (PUR) Shee, E. L.; Soo, J. B.; Soo, Y. K.; Young, R. K.; Joon, H. R. (2005) Effect of the heat shock protein 70 on the adjuvanticity induced by a bacterial flagellin of Vibrio vulnificus. Journal of Bacteriology and Virology 35(4): 299-305.
1048.  (APP) (EPL) (REV) Shekhtman, A. (2005) Protein chemical ligation as an invaluable tool for structural NMR. Protein Pept Lett 12(8): 765-8. PubMed ID: 16305546
1049.  (INT) (Structure) Shemella, P.; Pereira, B.; Zhang, Y.; Van Roey, P.; Belfort, G.; Garde, S.; Nayak, S. K. (2007) Mechanism for intein C-terminal cleavage: a proposal from quantum mechanical calculations. Biophys J 92(3): 847-53. PubMed ID: 17085503
1050.  (INT) (Structure) Shemella, P.; Pereira, B.; Garde, S..; Nayak, S. K.; Belfort, G. (2008) 2880-Pos Density Functional Study of Intein Reaction Mechanisms. Biophys. J. 94(1_MeetingAbstracts): 2880.
1051.  (INT) Shen, Y.; Musti, K.; Hiramoto, M.; Kikuchi, H.; Kawarabayashi, Y.; Matsui, I. (2001) Invariant Asp-1122 and Asp-1124 are essential residues for polymerization catalysis of family D DNA polymerase from Pyrococcus horikoshii. J Biol Chem 276(29): 27376-83. PubMed ID: 11319225
1052.  (APP) (EPL) Shen, K.; Cole, P. A. (2003) Conversion of a tyrosine kinase protein substrate to a high affinity ligand by ATP linkage. J Am Chem Soc. 125(52): 16172-3. PubMed ID: 14692742
1053.  (INT) Shen, Y.; Tang, X. F.; Matsui, I. (2003B) Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerase from Pyrococcus horikoshii. J Biol Chem 278(23): 21247-57. PubMed ID: 12654919
1054.  (INT) Shen, Y.; Tang, X. F.; Yokoyama, H.; Matsui, E.; Matsui, I. (2004) A 21-amino acid peptide from the cysteine cluster II of the family D DNA polymerase from Pyrococcus horikoshii stimulates its nuclease activity which is Mre11-like and prefers manganese ion as the cofactor. Nucleic Acids Res 32(1): 158-68. PubMed ID: 14704353
1055.  (INT) Shen, Y.; Tang, X. F.; Matsui, E.; Matsui, I. (2004B) Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerase from Pyrococcus horikoshii. Biochem Soc Trans 32(Pt 2): 245-9. PubMed ID: 15046581
1056.  (APP) (EPL) (REV) Shen, K.; Hines, A. C.; Schwarzer, D.; Pickin, K. A.; Cole, P. A. (2005) Protein kinase structure and function analysis with chemical tools. Biochim Biophys Acta 1754(1-2): 65-78. PubMed ID: 16213197
1057.  (APP) (EPL) (TAG) Shen, K. (2007) Analyzing protein tyrosine phosphatases by phosphotyrosine analog integration. Methods 42(3): 234-42. PubMed ID: 17532510
1058.  (APP) (PUR) Shen, Y.; Ai, H. X.; Song, R.; Liang, Z. N.; Li, J. F.; Zhang, S. Q. (2010) Expression and purification of moricin CM4 and human beta-defensins 4 in Escherichia coli using a new technology. Microbiol Res 165(8): 713-8. PubMed ID: 20089386
1059.  (APP) (CPS) (TS) Shi, J.; Muir, T. W. (2005) Development of a tandem protein trans-splicing system based on native and engineered split inteins. J Am Chem Soc 127(17): 6198-206. PubMed ID: 15853324
1060.  (APP) (PUR) Shi, G.; Yan, J.; Yue, C.; Qi, T.; Chen, S.; Zhang, Y. (2008) Expression of human alpha-defensin HNP-2 gene in Escherichia coli. Chinese Journal of Applied and Environmental Biology 14(1): 78-82.
1061.  (APP) (INT) (PUR) Shi, Y. F.; Soumillion, P.; Ueda, M. (2010) Effects of catalytic site mutations on active expression of phage fused penicillin acylase. J Biotechnol 145(2): 139-42. PubMed ID: 19932137
1062.  (APP) (PUR) Shier, V. K.; Hancey, C. J.; Benkovic, S. J. (2001) Identification of the active oligomeric state of an essential adenine DNA methyltransferase from Caulobacter crescentus. J Biol Chem 276(18): 14744-51. PubMed ID: 11278726
1063.  (INT) Shih, C. K.; Wagner, R.; Feinstein, S.; Kanik-Ennulat, C.; Neff, N. (1988) A dominant trifluoperazine resistance gene from Saccharomyces cerevisiae has homology with F0F1 ATP synthase and confers calcium-sensitive growth. Mol Cell Biol. 8(8): 3094-103. PubMed ID: 2905423
1064.  (EN) (INT) (TS) Shingledecker, K.; Jiang, S. Q.; Paulus, H. (1998) Molecular dissection of the Mycobacterium tuberculosis RecA intein: design of a minimal intein and of a trans-splicing system involving two intein fragments. Gene 207(2): 187-95. PubMed ID: 9511761
1065.  (INT) Shingledecker, K.; Jiang, S.; Paulus, H. (2000) Reactivity of the cysteine residues in the protein splicing active center of the Mycobacterium tuberculosis RecA intein. Arch Biochem Biophys 375(1): 138-44. PubMed ID: 10683259
1066.  (APP) (REV) Shogren-Knaak, M. A.; Alaimo, P. J.; Shokat, K. M. (2001) Recent advances in chemical approaches to the study of biological systems. Annu Rev Cell Dev Biol. 17: 405-33. PubMed ID: 11687495
1067.  (EN) (REV) Shub, D. A.; Goodrich-Blair, H. (1992) Protein introns: a new home for endonucleases. Cell. 71(2): 183-6. PubMed ID: 1330319
1068.  (APP) (CYCL) (REV) (TS) Siebold, C.; Flukiger, K.; Beutler, R.; Erni, B. (2001) Carbohydrate transporters of the bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS). FEBS Lett 504(3): 104-11. PubMed ID: 11532441
1069.  (APP) (CYCL) (TS) Siebold, C.; Erni, B. (2002) Intein-mediated cyclization of a soluble and a membrane protein in vivo: function and stability. Biophys Chem 96(2-3): 163-71. PubMed ID: 12034438
1070.  (EN) (INT) (REV) Siefert, J. L. (2009) Defining the mobilome. Methods Mol Biol 532: 13-27. PubMed ID: 19271177
1071.  (EN) Silva, G. H.; Belfort, M. (2004) Analysis of the LAGLIDADG interface of the monomeric homing endonuclease I-DmoI. Nucleic Acids Res 32(10): 3156-68. PubMed ID: 15190132
1072.  (EN) Silva, G. H.; Belfort, M.; Wende, W.; Pingoud, A. (2006) From monomeric to homodimeric endonucleases and back: engineering novel specificity of LAGLIDADG enzymes. J Mol Biol 361(4): 744-54. PubMed ID: 16872628
1073.  (APP) (PUR) (Structure) Sinars, C. R.; Cheung-Flynn, J.; Rimerman, R. A.; Scammell, J. G.; Smith, D. F.; Clardy, J. (2003) Structure of the large FK506-binding protein FKBP51, an Hsp90-binding protein and a component of steroid receptor complexes. Proc Natl Acad Sci U S A 100(3): 868-73. PubMed ID: 12538866
1074.  (INT) (REL) (REV) Singh, R.; Robida, M. D.; Karimpour, S. (2006) Building biological complexity with limited genes. Current Genomics 7(2): 97-114.
1075.  (APP) (PUR) Singh, P. K.; Gupta, M. N. (2008) Simultaneous refolding and purification of a recombinant lipase with an intein tag by affinity precipitation with chitosan. Biochim Biophys Acta 1784: 1825-1829. PubMed ID: 18725331
1076.  (EN) (INT) Singh, P.; Tripathi, P.; Silva, G. H.; Pingoud, A.; Muniyappa, K. (2009) Characterization of Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease, reveals a unique mode of DNA binding, helical distortion, and cleavage compared with a canonical LAGLIDADG homing endonuclease. J Biol Chem 284(38): 25912-28. PubMed ID: 19605345
1077.  (EN) Singh, P.; Tripathi, P.; Muniyappa, K. (2010) Mutational analysis of active-site residues in the Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease: Asp(122) and Asp(193) are crucial to the double-stranded DNA cleavage activity whereas Asp(218) is not. Protein Sci 19(1): 111-23. PubMed ID: 19937653
1078.  (APP) (EPL) Singla, N.; Himanen, J. P.; Muir, T. W.; Nikolov, D. B. (2008) Toward the semisynthesis of multidomain transmembrane receptors: modification of Eph tyrosine kinases. Protein Sci 17(10): 1740-7. PubMed ID: 18628240
1079.  (APP) (PUR) Singleton, S. F.; Simonette, R. A.; Sharma, N. C.; Roca, A. I. (2002) Intein-mediated affinity-fusion purification of the Escherichia coli RecA protein. Protein Expr Purif 26(3): 476-88. PubMed ID: 12460773
1080.  (APP) (REL) Skretas, Georgios; Wood, David W. (2005) A Bacterial Biosensor of Endocrine Modulators. Journal of Molecular Biology 349(3): 464-474.
1081.  (APP) (CPS) Skretas, G.; Wood, D. W. (2005B) Regulation of protein activity with small-molecule-controlled inteins. Protein Sci 14(2): 523-32. PubMed ID: 15632292
1082.  (APP) (CPS) Skretas, G.; Wood, D. W. (2005C) Rapid detection of subtype-selective nuclear hormone receptor binding with bacterial genetic selection. Appl Environ Microbiol 71(12): 8995-7. PubMed ID: 16332908
1083.  (APP) (CPS) (REV) Skretas, G.; Meligova, A. K.; Villalonga-Barber, C.; Mitsiou, D. J.; Alexis, M. N.; Micha-Screttas, M.; Steele, B. R.; Screttas, C. G.; Wood, D. W. (2007) Engineered chimeric enzymes as tools for drug discovery: generating reliable bacterial screens for the detection, discovery, and assessment of estrogen receptor modulators. J Am Chem Soc 129(27): 8443-57. PubMed ID: 17569534
1084.  (APP) (EPL) (NMR) (PUR) (Structure) Skrisovska, L.; Allain, F. H. (2008) Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L. J Mol Biol 375(1): 151-64. PubMed ID: 17936301
1085.  (APP) (EPL) (NMR) (REV) (TS) Skrisovska, L.; Schubert, M.; Allain, F. H. (2010) Recent advances in segmental isotope labeling of proteins: NMR applications to large proteins and glycoproteins. J Biomol NMR 46(1): 51-65. PubMed ID: 19690964
1086.  (INT) Slesarev, A. I.; Mezhevaya, K. V.; Makarova, K. S.; Polushin, N. N.; Shcherbinina, O. V.; Shakhova, V. V.; Belova, G. I.; Aravind, L.; Natale, D. A.; Rogozin, I. B.; Tatusov, R. L.; Wolf, Y. I.; Stetter, K. O.; Malykh, A. G.; Koonin, E. V.; Kozyavkin, S. A. (2002) The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens. Proc Natl Acad Sci U S A. 99(7): 4644-9. PubMed ID: 11930014
1087.  (INT) Smith, D. R.; Richterich, P.; Rubenfield, M.; Rice, P. W.; Butler, C.; Lee, H. M.; Kirst, S.; Gundersen, K.; Abendschan, K.; Xu, Q.; Chung, M.; Deloughery, C.; Aldredge, T.; Maher, J.; Lundstrom, R.; Tulig, C.; Falls, K.; Imrich, J.; Torrey, D.; Engelstein, M.; Breton, G.; Madan, D.; Nietupski, R.; Seitz, B.; Mao, J. I.; et al., (1997) Multiplex sequencing of 1.5 Mb of the Mycobacterium leprae genome. Genome Res 7(8): 802-19. PubMed ID: 9267804
1088.  (INT) Smith, D. R.; Doucette-Stamm, L. A.; Deloughery, C.; Lee, H.; Dubois, J.; Aldredge, T.; Bashirzadeh, R.; Blakely, D.; Cook, R.; Gilbert, K.; Harrison, D.; Hoang, L.; Keagle, P.; Lumm, W.; Pothier, B.; Qiu, D.; Spadafora, R.; Vicaire, R.; Wang, Y.; Wierzbowski, J.; Gibson, R.; Jiwani, N.; Caruso, A.; Bush, D.; Reeve, J. N.; et al., (1997B) Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics. J Bacteriol 179(22): 7135-55. PubMed ID: 9371463
1089.  (APP) Song, L. P.; Huang, H. L. (2003) [Protein splicing and its application]. Sheng Wu Gong Cheng Xue Bao 19(2): 249-54. PubMed ID: 15966332
1090.  (INT) Souciet, J. L.; Dujon, B.; Gaillardin, C.; Johnston, M.; Baret, P. V.; Cliften, P.; Sherman, D. J.; Weissenbach, J.; Westhof, E.; Wincker, P.; Jubin, C.; Poulain, J.; Barbe, V.; Segurens, B.; Artiguenave, F.; Anthouard, V.; Vacherie, B.; Val, M. E.; Fulton, R. S.; Minx, P.; Wilson, R.; Durrens, P.; Jean, G.; Marck, C.; Martin, T.; Nikolski, M.; Rolland, T.; Seret, M. L.; Casaregola, S.; Despons, L.; Fairhead, C.; Fischer, G.; Lafontaine, I.; Leh, V.; Lemaire, M.; de Montigny, J.; Neuveglise, C.; Thierry, A.; Blanc-Lenfle, I.; Bleykasten, C.; Diffels, J.; Fritsch, E.; Frangeul, L.; Goeffon, A.; Jauniaux, N.; Kachouri-Lafond, R.; Payen, C.; Potier, S.; Pribylova, L.; Ozanne, C.; Richard, G. F.; Sacerdot, C.; Straub, M. L.; Talla, E. (2009) Comparative genomics of protoploid Saccharomycetaceae. Genome Res 19(10): 1696-709. PubMed ID: 19525356
1091.  (INT) (NEB) Southworth, M. W.; Kong, H.; Kucera, R. B.; Ware, J.; Jannasch, H. W.; Perler, F. B. (1996) Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5' exonuclease activity. Proc Natl Acad Sci U S A 93(11): 5281-5. PubMed ID: 8643567
1092.  (NEB) (TS) Southworth, M. W.; Adam, E.; Panne, D.; Byer, R.; Kautz, R.; Perler, F. B. (1998) Control of protein splicing by intein fragment reassembly. Embo J 17(4): 918-26. PubMed ID: 9463370
1093.  (APP) (INT) (NEB) (PUR) Southworth, M. W.; Amaya, K.; Evans, T. C.; Xu, M. Q.; Perler, F. B. (1999) Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. Biotechniques 27(1): 110-4, 116, 118-20. PubMed ID: 10407673
1094.  (INT) (NEB) Southworth, M. W.; Benner, J.; Perler, F. B. (2000) An alternative protein splicing mechanism for inteins lacking an N-terminal nucleophile. Embo J 19(18): 5019-26. PubMed ID: 10990465
1095.  (INT) (NEB) Southworth, M. W.; Perler, F. B. (2002) Protein splicing of the Deinococcus radiodurans strain R1 Snf2 intein. J Bacteriol 184(22): 6387-8. PubMed ID: 12399510
1096.  (INT) (NEB) (REV) Southworth, M.W.; Perler, F.B. (2002B) Inteins in microbial genomes: distribution, mechanism and function. Scientific World Journal 2(Suppl 2): 25-6. PubMed ID: 14532441
1097.  (INT) (NEB) (REL) Southworth, M. W.; Yin, J.; Perler, F. B. (2004) Rescue of protein splicing activity from a Magnetospirillum magnetotacticum intein-like element. Biochem Soc Trans 32(Pt 2): 250-4. PubMed ID: 15046582
1098.  (APP) (PUR) Squire, C. M.; Goss, R. J.; Hong, H.; Leadlay, P. F.; Staunton, J. (2003) Catalytically active tetramodular 6-deoxyerythonolide B synthase fusion proteins. Chembiochem 4(11): 1225-8. PubMed ID: 14613116
1099.  (APP) (PUR) Srinivasa Babu, K.; Antony, A.; Muthukumaran, T.; Meenakshisundaram, S. (2008) Construction of intein-mediated hGMCSF expression vector and its purification in Pichia pastoris. Protein Expr Purif 57(2): 201-5. PubMed ID: 18309571
1100.  (APP) (PUR) Srinivasa Babu, K.; Muthukumaran, T.; Antony, A.; Prem Singh Samuel, S. D.; Balamurali, M.; Murugan, V.; Meenakshisundaram, S. (2009) Single step intein-mediated purification of hGMCSF expressed in salt-inducible E. coli. Biotechnol Lett 31(5): 659-64. PubMed ID: 19148582
1101.  (APP) (REV) Srinivasan, R.; Yao, S. Q.; Yeo, D. S. (2004) Chemical approaches for live cell bioimaging. Comb Chem High Throughput Screen 7(6): 597-604. PubMed ID: 15379631
1102.  (REL) Srinivasulu, S.; Acharya, A. S. (2002) Product-conformation-driven ligation of peptides by V8 protease. Protein Sci 11(6): 1384-92. PubMed ID: 12021437
1103.  (APP) (PUR) Staii, C.; Wood, D. W.; Scoles, G. (2008) Ligand-induced structural changes in maltose binding proteins measured by atomic force microscopy. Nano Lett 8(8): 2503-9. PubMed ID: 18642963
1104.  (APP) (PUR) Staii, C.; Wood, D. W.; Scoles, G. (2008) Verification of biochemical activity for proteins nanografted on gold surfaces. J Am Chem Soc 130(2): 640-6. PubMed ID: 18081283
1105.  (INT) Stankovic, S.; Soldo, B.; Beric-Bjedov, T.; Knezevic-Vukcevic, J.; Simic, D.; Lazarevic, V. (2007) Subspecies-specific distribution of intervening sequences in the Bacillus subtilis prophage ribonucleotide reductase genes. Syst Appl Microbiol 30(1): 8-15. PubMed ID: 16621400
1106.  (INT) (REV) Starokadomskyy, P.L. (2005) [Protein splicing]. Ukr Biokhim Zh. 77(4): 14-29.. PubMed ID: 16568601
1107.  (APP) (PUR) Starokadomskyy, P.L.; Okunev, O.V.; Dubey, L.V. (2006) Utilizing of protein splicing phenomenon for optimization of obtaining and purification of the recombinant human growth hormone. Europ J Medic Res 11: 128-129.
1108.  (REV) Starokadomskyy, P.L. (2007) Protein Splicing. Molecular Biology 41(2): 278-293.
1109.  (REV) Starokadomskyy, P.L. (2007B) [Protein splicing]. Mol Biol (Mosk) 41(2): 314-30. PubMed ID: 17514899
1110.  (APP) (PUR) Starokadomskyy, P.L.; Dubey, I.Ya.; Okunev, O.V.; Irodov, D. (2007C) [Intein-containing chimeric protein construction and evaluation of its cleavage]. Cytology and Genetics 41(2): 3-11.
1111.  (APP) (PUR) Starokadomskyy, P.L.; Dubey, I.Ya.; Okunev, O.V.; Irodov, D. (2007D) [Intein-containing chimeric protein construction and evaluation of its cleavage conditions]. Tsitol Genet 41(2): 3-11. PubMed ID: 17494337
1112.  (APP) (PUR) Starokadomskyy, P. L.; Okunev, O. V.; Irodov, D. M.; Kordium, V. A. (2008) Utilization of protein splicing for purification of the human growth hormone. Molecular Biology 42(6): 966-972.
1113.  (APP) (PUR) Starokadomskyy, P. L.; Okunev, O. V.; Irodov, D. M.; Kordium, V. A. (2008B) Utilizing of protein splicing for human growth hormone purification. Molekuliarnaia biologiia 42(6): 1085-1092.
1114.  (INT) (REV) Starokadomskyy, P. L. (2010) Protein splicing and its evolution in eukaryotes. Biopolymers and Cell 26(1): 2-12.
1115.  (APP) (PUR) Stasser, J. P.; Siluvai, G. S.; Barry, A. N.; Blackburn, N. J. (2007) A multinuclear copper(I) cluster forms the dimerization interface in copper-loaded human copper chaperone for superoxide dismutase. Biochemistry 46(42): 11845-56. PubMed ID: 17902702
1116.  (APP) (NMR) (REV) (Structure) Staunton, D.; Owen, J.; Campbell, I. D. (2003) NMR and structural genomics. Acc Chem Res. 36(3): 207-14. PubMed ID: 12641478
1117.  (APP) (PUR) Stefanidakis, M.; Bjorklund, M.; Ihanus, E.; Gahmberg, C. G.; Koivunen, E. (2003) Identification of a negatively charged peptide motif within the catalytic domain of progelatinases that mediates binding to leukocyte beta 2 integrins. J Biol Chem 278(36): 34674-84. PubMed ID: 12824186
1118.  (APP) (REL) (REV) Stennicke, H. R.; Salvesen, G. S. (2006) Chemical ligation--an unusual paradigm in protease inhibition. Mol Cell 21(6): 727-8. PubMed ID: 16543139
1119.  (APP) (PUR) Stepanenko, V. N.; Esipov, R. S.; Gurevich, A. I.; Chupova, L. A.; Miroshnikov, A. I. (2007B) Recombinant oxyntomodulin. Russian Journal of Bioorganic Chemistry 33(2): 227-232.
1120.  (APP) (PUR) Stepannenko, V. N.; Esipov, R. S.; Gurevich, A. I.; Chupova, L. A.; Miroshnikov, A. I. (2007) [Recombinant oxyntomodulin]. Bioorg Khim 33(2): 245-50. PubMed ID: 17476985
1121.  (REL) Stephenson, R. C.; Clarke, S. (1989) Succinimide formation from aspartyl and asparaginyl peptides as a model for the spontaneous degradation of proteins. J Biol Chem 264(11): 6164-70. PubMed ID: 2703484
1122.  (EN) (INT) Steuer, S.; Pingoud, V.; Pingoud, A.; Wende, W. (2004) Chimeras of the homing endonuclease PI-SceI and the homologous Candida tropicalis intein: a study to explore the possibility of exchanging DNA-binding modules to obtain highly specific endonucleases with altered specificity. Chembiochem 5(2): 206-13. PubMed ID: 14760742
1123.  (INT) (REV) Stoddard, B. L.; Pietrokovski, S. (1998) Breaking up is hard to do. Nat Struct Biol 5(1): 3-5. PubMed ID: 9437416
1124.  (EN) (REV) (Structure) Stoddard, B. L. (2006) Homing endonuclease structure and function. Q Rev Biophys 38(1): 49-95. PubMed ID: 16336743
1125.  (APP) (PUR) Stray, J. E.; Lindsley, J. E. (2003) Biochemical analysis of the yeast condensin Smc2/4 complex: an ATPase that promotes knotting of circular DNA. J Biol Chem 278(28): 26238-48. PubMed ID: 12719426
1126.  (APP) (PUR) (Structure) Stray, J. E.; Crisona, N. J.; Belotserkovskii, B. P.; Lindsley, J. E.; Cozzarelli, N. R. (2005) The Saccharomyces cerevisiae Smc2/4 condensin compacts DNA into (+) chiral structures without net supercoiling. J Biol Chem 280(41): 34723-34. PubMed ID: 16100111
1127.  (APP) (EPL) (REV) Strobel, S. A.; Muir, T. W. (2005) Biopolymers: Exploring biological function through chemical manipulation of biopolymers. Current Opinion in Chemical Biology 9(6): 545-547.
1128.  (APP) (PUR) Suarez, M.; Entenza, J. M.; Doerries, C.; Meyer, E.; Bourquin, L.; Sutherland, J.; Marison, I.; Moreillon, P.; Mermod, N. (2003) Expression of a plant-derived peptide harboring water-cleaning and antimicrobial activities. Biotechnol Bioeng 81(1): 13-20. PubMed ID: 12432576
1129.  (NEB) (TS) Sun, L.; Ghosh, I.; Paulus, H.; Xu, M. Q. (2001) Protein trans-splicing to produce herbicide-resistant acetolactate synthase. Appl Environ Microbiol 67(3): 1025-9. PubMed ID: 11229887
1130.  (APP) (EPL) (NEB) (TAG) Sun, L.; Ghosh, I.; Xu, M. Q. (2003) Generation of an affinity column for antibody purification by intein-mediated protein ligation. J Immunol Methods 282(1-2): 45-52. PubMed ID: 14604539
1131.  (APP) (TS) Sun, W.; Yang, J.; Liu, X. Q. (2004) Synthetic two-piece and three-piece split inteins for protein trans-splicing. J Biol Chem 279(34): 35281-6. PubMed ID: 15194682
1132.  (APP) (EPL) (NEB) (TAG) Sun, L.; Rush, J.; Ghosh, I.; Maunus, J. R.; Xu, M. Q. (2004B) Producing peptide arrays for epitope mapping by intein-mediated protein ligation. Biotechniques 37(3): 430-6, 438, 440 passim. PubMed ID: 15470898
1133.  (INT) (NEB) (Structure) Sun, P.; Ye, S.; Ferrandon, S.; Evans, T. C.; Xu, M. Q.; Rao, Z. (2005) Crystal structures of an intein from the split dnaE gene of Synechocystis sp. PCC6803 reveal the catalytic model without the penultimate histidine and the mechanism of zinc ion inhibition of protein splicing. J Mol Biol 353(5): 1093-105. PubMed ID: 16219320
1134.  (APP) (PUR) Sun, Z.; Chen, J.; Yao, H.; Liu, L.; Wang, J.; Zhang, J.; Liu, J. N. (2005) Use of Ssp dnaB derived mini-intein as a fusion partner for production of recombinant human brain natriuretic peptide in Escherichia coli. Protein Expr Purif 43(1): 26-32. PubMed ID: 15979896
1135.  (APP) (EPL) (NEB) (TAG) Sun, L.; Ghosh, I.; Barshevsky, T.; Kochinyan, S.; Xu, M. Q. (2007) Design, preparation and use of ligated phosphoproteins: A novel approach to study protein phosphatases by dot blot array, ELISA and Western blot assays. Methods 42(3): 220-6. PubMed ID: 17532508
1136.  (EN) Sussman, D.; Chadsey, M.; Fauce, S.; Engel, A.; Bruett, A.; Monnat, R., Jr.; Stoddard, B. L.; Seligman, L. M. (2004) Isolation and characterization of new homing endonuclease specificities at individual target site positions. J Mol Biol 342(1): 31-41. PubMed ID: 15313605
1137.  (INT) Swithers, K. S.; Senejani, A. G.; Fournier, G. P.; Gogarten, J. P. (2009) Conservation of intron and intein insertion sites: implications for life histories of parasitic genetic elements. BMC Evol Biol 9: 303. PubMed ID: 20043855
1138.  (APP) (EPL) Sydor, J. R.; Mariano, M.; Sideris, S.; Nock, S. (2002) Establishment of intein-mediated protein ligation under denaturing conditions: C-terminal labeling of a single-chain antibody for biochip screening. Bioconjug Chem 13(4): 707-12. PubMed ID: 12121124
1139.  (APP) (EPL) (TAG) Szewczuk, L. M.; Tarrant, M. K.; Sample, V.; Drury, W. J., 3rd; Zhang, J.; Cole, P. A. (2008) Analysis of serotonin N-acetyltransferase regulation in vitro and in live cells using protein semisynthesis. Biochemistry 47(39): 10407-19. PubMed ID: 18771288
1140.  (APP) (EPL) (REV) (TAG) Szewczuk, L. M.; Tarrant, M. K.; Cole, P. A. (2009) Protein phosphorylation by semisynthesis: from paper to practice. Methods Enzymol 462: 1-24. PubMed ID: 19632467
1141.  (APP) (PUR) Szweda, P.; Pladzyk, R.; Kotlowski, R.; Kur, J. (2001) Cloning, expression, and purification of the Staphylococcus simulans lysostaphin using the intein-chitin-binding domain (CBD) system. Protein Expr Purif 22(3): 467-71. PubMed ID: 11483010
1142.  (REL) Tajima, N.; Kawai, F.; Park, S. Y.; Tame, J. R. (2010) A novel intein-like autoproteolytic mechanism in autotransporter proteins. J Mol Biol 402(4): 645-56. PubMed ID: 20615416
1143.  (INT) Takagi, M.; Nishioka, M.; Kakihara, H.; Kitabayashi, M.; Inoue, H.; Kawakami, B.; Oka, M.; Imanaka, T. (1997) Characterization of DNA polymerase from Pyrococcus sp. strain KOD1 and its application to PCR. Appl Environ Microbiol 63(11): 4504-10. PubMed ID: 9361436
1144.  (APP) (CYCL) (REL) Takahashi, H.; Arai, M.; Takenawa, T.; Sota, H.; Xie, Q. H.; Iwakura, M. (2007) Stabilization of hyperactive dihydrofolate reductase by cyanocysteine-mediated backbone cyclization. J Biol Chem 282(13): 9420-9. PubMed ID: 17264073
1145.  (APP) (EPL) (TAG) Takeda, S.; Tsukiji, S.; Nagamune, T. (2004) Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation. Bioorg Med Chem Lett 14(10): 2407-10. PubMed ID: 15109622
1146.  (APP) (REL) Takeda, Shuji; Tsukiji, Shinya; Nagamune, Teruyuki (2005) A cysteine-appended deoxyuridine for the postsynthetic DNA modification using native chemical ligation. Tetrahedron Letters 46(13): 2235-2238.
1147.  (APP) (REV) Takeuchi, M.; Ozawa, T. (2006) Frontiers in bioimaging. ACS Chem Biol 1(6): 333-4. PubMed ID: 17163765
1148.  (APP) (PUR) (REV) (TAG) Takeuchi, M.; Ozawa, T. (2007) Methods for imaging and analyses of intracellular organelles using fluorescent and luminescent proteins. Anal Sci 23(1): 25-9. PubMed ID: 17213619
1149.  (REL) Tam, J. P.; Yu, Q. (1998) Methionine ligation strategy in the biomimetic synthesis of parathyroid hormones. Biopolymers 46(5): 319-27. PubMed ID: 9754028
1150.  (EPL) (REV) Tam, J. P. (1999) Ligation and splicing of peptides and proteins. Biopolymers 51(5): 309-10. PubMed ID: 10685042
1151.  (APP) (EPL) (REL) Tam, J. P.; Yu, Q.; Miao, Z. (1999B) Orthogonal ligation strategies for peptide and protein. Biopolymers. 51(5): 311-32. PubMed ID: 10685043
1152.  (APP) (REL) Tam, J. P.; Yu, Q.; Yang, J. L. (2001) Tandem ligation of unprotected peptides through thiaprolyl and cysteinyl bonds in water. J Am Chem Soc. 123(11): 2487-94. PubMed ID: 11456916
1153.  (REL) Tam, J. P.; Eom, K. D. (2005) Mimicking reverse protein splicing by three-segment tandem peptide ligation. Protein Pept Lett 12(8): 743-9. PubMed ID: 16305543
1154.  (APP) (EPL) (REL) Tam, A.; Soellner, M. B.; Raines, R. T. (2007) Water-soluble phosphinothiols for traceless staudinger ligation and integration with expressed protein ligation. J Am Chem Soc 129(37): 11421-30. PubMed ID: 17713909
1155.  (APP) (EPL) Tan, Y.; Ruan, H.; Demeter, M. R.; Comb, M. J. (1999) p90(RSK) blocks bad-mediated cell death via a protein kinase C-dependent pathway. J Biol Chem 274(49): 34859-67. PubMed ID: 10574959
1156.  (APP) (EPL) (TAG) Tan, L. P.; Lue, R. Y.; Chen, G. Y.; Yao, S. Q. (2004) Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo. Bioorg Med Chem Lett 14(24): 6067-70. PubMed ID: 15546731
1157.  (APP) (PUR) (TAG) Tan, L. P.; Chen, G. Y.; Yao, S. Q. (2004B) Expanding the scope of site-specific protein biotinylation strategies using small molecules. Bioorg Med Chem Lett 14(23): 5735-8. PubMed ID: 15501031
1158.  (APP) (REV) (TAG) Tan, L. P.; Yao, S. Q. (2005) Intein-mediated, in vitro and in vivo protein modifications with small molecules. Protein Pept Lett 12(8): 769-75. PubMed ID: 16305547
1159.  (APP) (CPS) Tan, G.; Chen, M.; Foote, C.; Tan, C. (2009) Temperature-sensitive mutations made easy: generating conditional mutations by using temperature-sensitive inteins that function within different temperature ranges. Genetics 183(1): 13-22. PubMed ID: 19596904
1160.  (INT) Tang, X. F.; Shen, Y.; Matsui, E.; Matsui, I. (2004) Domain topology of the DNA polymerase D complex from a hyperthermophilic archaeon Pyrococcus horikoshii. Biochemistry 43(37): 11818-27. PubMed ID: 15362867
1161.  (APP) (EPL) Tann, C. M.; Qi, D.; Distefano, M. D. (2001) Enzyme design by chemical modification of protein scaffolds. Curr Opin Chem Biol. 5(6): 696-704. PubMed ID: 11738181
1162.  (APP) (CYCL) Tavassoli, A.; Benkovic, S. J. (2005) Genetically selected cyclic-peptide inhibitors of AICAR transformylase homodimerization. Angew Chem Int Ed Engl 44(18): 2760-3. PubMed ID: 15830403
1163.  (APP) (CYCL) (REV) Tavassoli, A.; Naumann, T.A.; Benkovic, S.J. (2005B) Production of Cyclic Proteins and Peptides. In: Homing Endonucleases and Inteins, ed. Belfort, M.; Derbyshire, V.; Stoddard, B.L.; Wood, D.W.. (Springer-Verlag, Heidelberg, Germany) Vol. 16 pages 293-306.
1164.  (APP) (CYCL) (REV) Tavassoli, A.; Benkovic, S. J. (2007) Split-intein mediated circular ligation used in the synthesis of cyclic peptide libraries in E. coli. Nat Protoc 2(5): 1126-33. PubMed ID: 17546003
1165.  (APP) (CYCL) Tavassoli, A.; Lu, Q.; Gam, J.; Pan, H.; Benkovic, S. J.; Cohen, S. N. (2008) Inhibition of HIV budding by a genetically selected cyclic peptide targeting the Gag-TSG101 interaction. ACS Chem Biol 3(12): 757-64. PubMed ID: 19053244
1166.  (REL) Teixeira, M. T.; Siniossoglou, S.; Podtelejnikov, S.; Benichou, J. C.; Mann, M.; Dujon, B.; Hurt, E.; Fabre, E. (1997) Two functionally distinct domains generated by in vivo cleavage of Nup145p: a novel biogenesis pathway for nucleoporins. Embo J. 16(16): 5086-97. PubMed ID: 9305650
1167.  (REL) Teixeira, M. T.; Fabre, E.; Dujon, B. (1999) Self-catalyzed cleavage of the yeast nucleoporin Nup145p precursor. J Biol Chem. 274(45): 32439-44. PubMed ID: 10542288
1168.  (INT) (NEB) Telenti, A.; Southworth, M.; Alcaide, F.; Daugelat, S.; Jacobs, W. R., Jr.; Perler, F. B. (1997) The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein. J Bacteriol 179(20): 6378-82. PubMed ID: 9335286
1169.  (PUR) (REL) (REV) (TAG) Terpe, K. (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol 60(5): 523-33. PubMed ID: 12536251
1170.  (APP) (EPL) (TAG) Teruya, K.; Nishizawa, K.; Doh-ura, K. (2010) Semisynthesis of a protein with cholesterol at the C-terminal, targeted to the cell membrane of live cells. Protein J 29(7): 493-500. PubMed ID: 20814724
1171.  (INT) Theodoro, R. C.; Bagagli, E.; Oliveira, C. (2008) Phylogenetic analysis of PRP8 intein in Paracoccidioides brasiliensis species complex. Fungal Genet Biol 45(9): 1284-91. PubMed ID: 18672080
1172.  (INT) Theodoro, R. C.; Bagagli, E. (2009) Inteins in pathogenic fungi: a phylogenetic tool and perspectives for therapeutic applications. Mem Inst Oswaldo Cruz 104(3): 497-504. PubMed ID: 19547879
1173.  (EN) (INT) Theodoro, R. C.; Volkmann, G.; Liu, X. Q.; Bagagli, E. (2010) PRP8 intein in Ajellomycetaceae family pathogens: Sequence analysis, splicing evaluation and homing endonuclease activity. Fungal Genet Biol (IN PRESS). PubMed ID: 20682355
1174.  (EN) (INT) Thion, L.; Laurine, E.; Erard, M.; Burlet-Schiltz, O.; Monsarrat, B.; Masson, J. M.; Saves, I. (2002) The two-step cleavage activity of PI-TfuI intein endonuclease demonstrated by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. J Biol Chem 277(47): 45442-50. PubMed ID: 12239210
1175.  (APP) (PUR) (Structure) Thoden, J. B.; Timson, D. J.; Reece, R. J.; Holden, H. M. (2004) Molecular structure of human galactose mutarotase. J Biol Chem 279(22): 23431-7. PubMed ID: 15026423
1176.  (APP) (EPL) Thompson, P. R.; Wang, D.; Wang, L.; Fulco, M.; Pediconi, N.; Zhang, D.; An, W.; Ge, Q.; Roeder, R. G.; Wong, J.; Levrero, M.; Sartorelli, V.; Cotter, R. J.; Cole, P. A. (2004) Regulation of the p300 HAT domain via a novel activation loop. Nat Struct Mol Biol 11(4): 308-15. PubMed ID: 15004546
1177.  (APP) (PUR) Thomson, C. A.; Ananthanarayanan, V. S. (2001) A method for expression and purification of soluble, active Hsp47, a collagen-specific molecular chaperone. Protein Expr Purif 23(1): 8-13. PubMed ID: 11570840
1178.  (APP) (EN) Thomson, J. M.; Lafayette, P. R.; Schmidt, M. A.; Parrott, W. A. (2002) Artificial gene-clusters engineered into plants using a vector system based on intron- and intein-encoded endonucleases. In Vitro Cellular and Developmental Biology - Plant 38(6): 537-542.
1179.  (REL) (REV) Thony-Meyer, L.; Bock, A.; Hennecke, H. (1992) Prokaryotic polyprotein precursors. FEBS Lett. 307(1): 62-5. PubMed ID: 1639196
1180.  (REL) Tinel, A.; Janssens, S.; Lippens, S.; Cuenin, S.; Logette, E.; Jaccard, B.; Quadroni, M.; Tschopp, J. (2007) Autoproteolysis of PIDD marks the bifurcation between pro-death caspase-2 and pro-survival NF-kappaB pathway. Embo J 26(1): 197-208. PubMed ID: 17159900
1181.  (INT) (REL) (REV) Tippin, B.; Pham, P.; Goodman, M. F. (2004) Error-prone replication for better or worse. Trends Microbiol 12(6): 288-95. PubMed ID: 15165607
1182.  (APP) (EPL) (TAG) Tolbert, T.J.; Wong, C-H (2000) Intein-Mediated Synthesis of Proteins Containing Carbohydrates and Other Molecular Probes. J Am Chem Soc 122: 5421-5428.
1183.  (REL) (Structure) Tolbert, W. D.; Zhang, Y.; Cottet, S. E.; Bennett, E. M.; Ekstrom, J. L.; Pegg, A. E.; Ealick, S. E. (2003) Mechanism of human S-adenosylmethionine decarboxylase proenzyme processing as revealed by the structure of the S68A mutant. Biochemistry 42(8): 2386-95. PubMed ID: 12600205
1184.  (INT) Tomaru, Y.; Shirai, Y.; Nagasaki, K. (2008) Ecology, physiology and genetics of a phycodnavirus infecting the noxious bloom-forming raphidophyte Heterosigma akashiwo. Fisheries Science 74(4): 701-711.
1185.  (INT) (NEB) Tori, K.; Dassa, B.; Johnson, M. A.; Southworth, M. W.; Brace, L. E.; Ishino, Y.; Pietrokovski, S.; Perler, F. B. (2010) Splicing of the mycobacteriophage Bethlehem DnaB intein: identification of a new mechanistic class of inteins that contain an obligate block F nucleophile. J Biol Chem 285(4): 2515-26. PubMed ID: 19940146
1186.  (APP) (PUR) Toulokhonov, I.; Artsimovitch, I.; Landick, R. (2001) Allosteric control of RNA polymerase by a site that contacts nascent RNA hairpins. Science 292(5517): 730-3. PubMed ID: 11326100
1187.  (APP) (CYCL) (REV) Trabi, M.; Craik, D. J. (2002) Circular proteins--no end in sight. Trends Biochem Sci. 27(3): 132-8. PubMed ID: 11893510
1188.  (APP) (INT) (REV) Travis, J. (1999) Making the Cut, Scientists exploit proteins that perform surgery upon themselves.. Science News 156: 222-223.
1189.  (INT) Turmel, M.; Brouard,J.-S.; Gagnon,C.; Otis, C.; Lemieux, C. (2008) Deep division in the Chlorophyceae (Chlorophyta) revealed by chloroplast phylogenomic analyses. J Phycol 44(3): 739-750.
1190.  (INT) Tyson, G. W.; Chapman, J.; Hugenholtz, P.; Allen, E. E.; Ram, R. J.; Richardson, P. M.; Solovyev, V. V.; Rubin, E. M.; Rokhsar, D. S.; Banfield, J. F. (2004) Community structure and metabolism through reconstruction of microbial genomes from the environment. Nature. 428(6978): 37-43. Epub 2004 Feb 1. PubMed ID: 14961025
1191.  (APP) (CPS) (TS) Tyszkiewicz, A. B.; Muir, T. W. (2008) Activation of protein splicing with light in yeast. Nat Methods 5(4): 303-5. PubMed ID: 18272963
1192.  (APP) (EPL) (TAG) Uhlenheuer, D. A.; Wasserberg, D.; Nguyen, H.; Zhang, L.; Blum, C.; Subramaniam, V.; Brunsveld, L. (2009) Modulation of protein dimerization by a supramolecular host-guest system. Chemistry 15(35): 8779-90. PubMed ID: 19621392
1193.  (APP) (REV) (TAG) (TS) Umezawa, Y. (2002) Assay and screening methods for bioactive substances based on cellular signaling pathways. J Biotechnol 82(4): 357-70. PubMed ID: 11996216
1194.  (APP) (PUR) Valentine, A. M.; Ishmael, F. T.; Shier, V. K.; Benkovic, S. J. (2001) A zinc ribbon protein in DNA replication: primer synthesis and macromolecular interactions by the bacteriophage T4 primase. Biochemistry 40(50): 15074-85. PubMed ID: 11735390
1195.  (REL) Valero, M. L.; Camarero, J. A.; Haack, T.; Mateu, M. G.; Domingo, E.; Giralt, E.; Andreu, D. (2000) Native-like cyclic peptide models of a viral antigenic site: finding a balance between rigidity and flexibility. J Mol Recognit 13(1): 5-13. PubMed ID: 10679891
1196.  (APP) (EPL) Valiyaveetil, F. I.; MacKinnon, R.; Muir, T. W. (2002) Semisynthesis and folding of the potassium channel KcsA. J Am Chem Soc 124(31): 9113-20. PubMed ID: 12149015
1197.  (APP) (EPL) Valiyaveetil, F. I.; Sekedat, M.; Mackinnon, R.; Muir, T. W. (2004) Glycine as a D-amino acid surrogate in the K(+)-selectivity filter. Proc Natl Acad Sci U S A 101(49): 17045-9. PubMed ID: 15563591
1198.  (APP) (EPL) Valiyaveetil, F. I.; Sekedat, M.; Muir, T. W.; MacKinnon, R. (2004) Semisynthesis of a functional K+ channel. Angew Chem Int Ed Engl. 43(19): 2504-7. PubMed ID: 15127436
1199.  (APP) (EPL) Valiyaveetil, F. I.; Sekedat, M.; MacKinnon, R.; Muir, T. W. (2006) Structural and functional consequences of an amide-to-ester substitution in the selectivity filter of a potassium channel. J Am Chem Soc 128(35): 11591-9. PubMed ID: 16939283
1200.  (APP) (EPL) Valiyaveetil, F. I.; Leonetti, M.; Muir, T. W.; Mackinnon, R. (2006B) Ion selectivity in a semisynthetic K+ channel locked in the conductive conformation. Science 314(5801): 1004-7. PubMed ID: 17095703
1201.  (INT) van der Wilk, F.; Dullemans, A. M.; Verbeek, M.; van den Heuvel, J. F. (1999) Isolation and characterization of APSE-1, a bacteriophage infecting the secondary endosymbiont of Acyrthosiphon pisum. Virology. 262(1): 104-13. PubMed ID: 10489345
1202.  (EN) (REL) (Structure) Van Roey, P.; Meehan, L.; Kowalski, J. C.; Belfort, M.; Derbyshire, V. (2002) Catalytic domain structure and hypothesis for function of GIY-YIG intron endonuclease I-TevI. Nat Struct Biol 9(11): 806-11. PubMed ID: 12379841
1203.  (INT) (Structure) Van Roey, P.; Pereira, B.; Li, Z.; Hiraga, K.; Belfort, M.; Derbyshire, V. (2007) Crystallographic and Mutational Studies of Mycobacterium tuberculosis recA Mini-inteins Suggest a Pivotal Role for a Highly Conserved Aspartate Residue. J Mol Biol 367(1): 162-73. PubMed ID: 17254599
1204.  (APP) (PUR) Vann, W. F.; Daines, D. A.; Murkin, A. S.; Tanner, M. E.; Chaffin, D. O.; Rubens, C. E.; Vionnet, J.; Silver, R. P. (2004) The NeuC protein of Escherichia coli K1 is a UDP N-acetylglucosamine 2-epimerase. J Bacteriol 186(3): 706-12. PubMed ID: 14729696
1205.  (APP) Varshney, A.; Mathew, M. K. (2000) Cytoplasmic residues influence the voltage-dependence of the gating of human K+ channels. Neuroreport 11(13): 2913-7. PubMed ID: 11006965
1206.  (APP) (NMR) (REV) (Structure) Venters, R.A.; Thompson, R.; Cavanagh, J. (2002) Current approaches for the study of large proteins by NMR. J Mol Structure 602: 275-292.
1207.  (REL) Vigneron, N.; Stroobant, V.; Chapiro, J.; Ooms, A.; Degiovanni, G.; Morel, S.; van der Bruggen, P.; Boon, T.; Van den Eynde, B. J. (2004) An antigenic peptide produced by peptide splicing in the proteasome. Science 304(5670): 587-90. PubMed ID: 15001714
1208.  (APP) (EPL) (TAG) Vila-Perello, M.; Pratt, M. R.; Tulin, F.; Muir, T. W. (2007) Covalent capture of phospho-dependent protein oligomerization by site-specific incorporation of a diazirine photo-cross-linker. J Am Chem Soc 129(26): 8068-9. PubMed ID: 17567014
1209.  (APP) (CPS) (INT) (TS) Vila-Perello, M.; Hori, Y.; Ribo, M.; Muir, T. W. (2008) Activation of protein splicing by protease- or light-triggered O to N acyl migration. Angew Chem Int Ed Engl 47(40): 7764-7. PubMed ID: 18767096
1210.  (APP) (CPS) (CYCL) (EPL) (REV) (TS) Vila-Perello, M.; Muir, T. W. (2010) Biological applications of protein splicing. Cell 143(2): 191-200. PubMed ID: 20946979
1211.  (APP) (EPL) (TAG) Virdee, S.; Ye, Y.; Nguyen, D. P.; Komander, D.; Chin, J. W. (2010) Engineered diubiquitin synthesis reveals Lys29-isopeptide specificity of an OTU deubiquitinase. Nat Chem Biol 6(10): 750-7. PubMed ID: 20802491
1212.  (APP) (EPL) (NMR) (PUR) (Structure) Vitali, F.; Henning, A.; Oberstrass, F. C.; Hargous, Y.; Auweter, S. D.; Erat, M.; Allain, F. H. (2006) Structure of the two most C-terminal RNA recognition motifs of PTB using segmental isotope labeling. EMBO J 25(1): 150-62. PubMed ID: 16362043
1213.  (REV) (TS) Vogel, G. (1998) A two-piece protein assembles itself. Science 281(5378): 763-4. PubMed ID: 9714677
1214.  (APP) (CYCL) (PUR) (TS) Volkmann, Gerrit; Murphy, Peter W.; Rowland, Elden E.; Liu, Xiang-Qin; Byers, David M. (2009) Intein-mediated creation of a cyclic acyl carrier protein. FASEB J._MeetingAbstracts 23(1_MeetingAbstracts): 850.8-.
1215.  (APP) (TAG) (TS) Volkmann, Gerrit; Liu, Xiang-Qin (2009B) C-terminal labelling of recombinant proteins using an engineered split-intein. FASEB J._MeetingAbstracts 23(1_MeetingAbstracts): 863.7.
1216.  (APP) (TS) Volkmann, G.; Sun, W.; Liu, X. Q. (2009C) Controllable protein cleavages through intein fragment complementation. Protein Sci 18(11): 2393-402. PubMed ID: 19768808
1217.  (APP) (TAG) (TS) Volkmann, G.; Liu, X. Q. (2009D) Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein. PLoS One 4(12): e8381. PubMed ID: 20027230
1218.  (APP) (CYCL) (PUR) Volkmann, G.; Murphy, P. W.; Rowland, E. E.; Cronan, J. E., Jr.; Liu, X. Q.; Blouin, C.; Byers, D. M. (2010) Intein-mediated cyclization of bacterial acyl carrier protein stabilizes its folded conformation but does not abolish function. J Biol Chem 285(12): 8605-14. PubMed ID: 20083605
1219.  (APP) (REV) (Structure) Volkmann, G.; Iwai, H. (2010B) Protein trans-splicing and its use in structural biology: opportunities and limitations. Mol Biosyst 6(11): 2110-21. PubMed ID: 20820635
1220.  (APP) (EPL) von Eggelkraut-Gottanka, R.; Machova, Z.; Grouzmann, E.; Beck-Sickinger, A. G. (2003) Semisynthesis and characterization of the first analogues of pro-neuropeptide Y. Chembiochem 4(5): 425-33. PubMed ID: 12740814
1221.  (APP) (EPL) von Eggelkraut-Gottanka, R.; Beck-Sickinger, A. G. (2004) Biosynthesis of peptide hormones derived from precursor sequences. Curr Med Chem 11(20): 2651-65. PubMed ID: 15544467
1222.  (REL) Voorter, C. E.; de Haard-Hoekman, W. A.; van den Oetelaar, P. J.; Bloemendal, H.; de Jong, W. W. (1988) Spontaneous peptide bond cleavage in aging alpha-crystallin through a succinimide intermediate. J Biol Chem. 263(35): 19020-3. PubMed ID: 3198609
1223.  (APP) (NMR) (REV) (TS) Wagner, G. (2010) Editorial: A topical issue: Production and labeling of biological macromolecules for NMR investigations. Journal of Biomolecular NMR 46(1): 1-2.
1224.  (APP) (PUR) (Structure) Wahl, M. C.; Huber, R.; Prade, L.; Marinkovic, S.; Messerschmidt, A.; Clausen, T. (1997) Cloning, purification, crystallization, and preliminary X-ray diffraction analysis of cystathionine gamma-synthase from E. coli. FEBS Lett 414(3): 492-6. PubMed ID: 9323022
1225.  (INT) (REV) Wallace, C. J. (1993) The curious case of protein splicing: mechanistic insights suggested by protein semisynthesis. Protein Sci 2(5): 697-705. PubMed ID: 8495192
1226.  (APP) (EPL) (REV) Wallace, C. J. (1995) Peptide ligation and semisynthesis. Curr Opin Biotechnol. 6(4): 403-10. PubMed ID: 7579650
1227.  (INT) (REL) (REV) Walsh, C. T.; Garneau-Tsodikova, S.; Gatto, G. J., Jr. (2005) Protein posttranslational modifications: the chemistry of proteome diversifications. Angew Chem Int Ed Engl 44(45): 7342-72. PubMed ID: 16267872
1228.  (APP) (EPL) (NMR) (PUR) (Structure) Walters, K. J.; Lech, P. J.; Goh, A. M.; Wang, Q.; Howley, P. M. (2003) DNA-repair protein hHR23a alters its protein structure upon binding proteasomal subunit S5a. Proc Natl Acad Sci U S A 100(22): 12694-9. PubMed ID: 14557549
1229.  (INT) Wang, S.; Liu, X. Q. (1997) Identification of an unusual intein in chloroplast ClpP protease of Chlamydomonas eugametos. J Biol Chem 272(18): 11869-73. PubMed ID: 9115246
1230.  (APP) (EPL) Wang, D.; Cole, P. A. (2001) Protein tyrosine kinase Csk-catalyzed phosphorylation of Src containing unnatural tyrosine analogues. J Am Chem Soc 123(37): 8883-6. PubMed ID: 11552794
1231.  (APP) (NEB) (PUR) (TAG) Wang, H.; Chong, S. (2003) Visualization of coupled protein folding and binding in bacteria and purification of the heterodimeric complex. Proc Natl Acad Sci U S A. 100(2): 478-83. Epub 2003 Jan 6. PubMed ID: 12515863
1232.  (APP) (PUR) Wang, Z. C.; Teng, Z. P.; Zhang, X. G.; Guo, X. C.; Yuan, J. M.; Zeng, Y. (2003) Obtaining purified p24 gag protein of HIV-1 by using a new prokaryotic expressive vector. Chinese Journal of Microbiology and Immunology 23(5): 375-379.
1233.  (APP) (REV) (TAG) Wang, H.; Chen, X. (2008) Site-specifically modified fusion proteins for molecular imaging. Front Biosci 13: 1716-32. PubMed ID: 17981663
1234.  (APP) (PUR) (TAG) Wang, Z.; Wu, H.; Chen, J.; Zhang, J.; Yao, Y.; Chen, G. Q. (2008B) A novel self-cleaving phasin tag for purification of recombinant proteins based on hydrophobic polyhydroxyalkanoate nanoparticles. Lab Chip 8(11): 1957-62. PubMed ID: 18941699
1235.  (APP) (PUR) (REV) Wang, L.; Kang, J. H.; Kim, K. H.; Lee, E. K. (2010) Expression of intein-tagged fusion protein and its applications in downstream processing. Journal of Chemical Technology and Biotechnology 85(1): 11-18.
1236.  (APP) (PUR) Wang, P. Z.; Gu, J. B.; Luo, J.; Peng, H. J. (2010B) [Antibacterial activity of recombinant thanatin expressed by E.coli ER2566.]. Nan Fang Yi Ke Da Xue Xue Bao 30(5): 1050-4. PubMed ID: 20501392
1237.  (APP) Wang, C. F.; Jiang, X. Y.; Gao, Y. H.; Li, G. M.; Guo, H. Y.; Hao, Wang; Qian, A. D. (2010C) Comparison of eleven single-round and multiple PCR assays targeting IS6110, IS1081, recA intein and ppsl intein gene for detection of Mycobacterium tuberculosis. In: 2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010. (, Chengdu) pages (IN PRESS).
1238.  (INT) Ward, N.; Larsen, O.; Sakwa, J.; Bruseth, L.; Khouri, H.; Durkin, A. S.; Dimitrov, G.; Jiang, L.; Scanlan, D.; Kang, K. H.; Lewis, M.; Nelson, K. E.; Methe, B.; Wu, M.; Heidelberg, J. F.; Paulsen, I. T.; Fouts, D.; Ravel, J.; Tettelin, H.; Ren, Q.; Read, T.; DeBoy, R. T.; Seshadri, R.; Salzberg, S. L.; Jensen, H. B.; Birkeland, N. K.; Nelson, W. C.; Dodson, R. J.; Grindhaug, S. H.; Holt, I.; Eidhammer, I.; Jonasen, I.; Vanaken, S.; Utterback, T.; Feldblyum, T. V.; Fraser, C. M.; Lillehaug, J. R.; Eisen, J. A. (2004) Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath). PLoS Biol. 2(10): e303. Epub 2004 Sep 21. PubMed ID: 15383840
1239.  (REL) Warren, E. H.; Vigneron, N. J.; Gavin, M. A.; Coulie, P. G.; Stroobant, V.; Dalet, A.; Tykodi, S. S.; Xuereb, S. M.; Mito, J. K.; Riddell, S. R.; Van den Eynde, B. J. (2006) An antigen produced by splicing of noncontiguous peptides in the reverse order. Science 313(5792): 1444-7. PubMed ID: 16960008
1240.  (INT) Waters, E.; Hohn, M. J.; Ahel, I.; Graham, D. E.; Adams, M. D.; Barnstead, M.; Beeson, K. Y.; Bibbs, L.; Bolanos, R.; Keller, M.; Kretz, K.; Lin, X.; Mathur, E.; Ni, J.; Podar, M.; Richardson, T.; Sutton, G. G.; Simon, M.; Soll, D.; Stetter, K. O.; Short, J. M.; Noordewier, M. (2003) The genome of Nanoarchaeum equitans: insights into early archaeal evolution and derived parasitism. Proc Natl Acad Sci U S A. 100(22): 12984-8. Epub 2003 Oct 17. PubMed ID: 14566062
1241.  (APP) (EPL) (TAG) Watzke, A.; Gutierrez-Rodriguez, M.; Kohn, M.; Wacker, R.; Schroeder, H.; Breinbauer, R.; Kuhlmann, J.; Alexandrov, K.; Niemeyer, C. M.; Goody, R. S.; Waldmann, H. (2006) A generic building block for C- and N-terminal protein-labeling and protein-immobilization. Bioorg Med Chem 14(18): 6288-306. PubMed ID: 16725326
1242.  (PUR) (REL) (REV) (TAG) Waugh, D. S. (2005) Making the most of affinity tags. Trends Biotechnol 23(6): 316-20. PubMed ID: 15922084
1243.  (APP) (EPL) (PUR) (REL) Weeks, S. D.; Drinker, M.; Loll, P. J. (2007) Ligation independent cloning vectors for expression of SUMO fusions. Protein Expr Purif 53(1): 40-50. PubMed ID: 17251035
1244.  (INT) Wei, J.; Goldberg, M. B.; Burland, V.; Venkatesan, M. M.; Deng, W.; Fournier, G.; Mayhew, G. F.; Plunkett, G., 3rd; Rose, D. J.; Darling, A.; Mau, B.; Perna, N. T.; Payne, S. M.; Runyen-Janecky, L. J.; Zhou, S.; Schwartz, D. C.; Blattner, F. R. (2003) Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T. Infect Immun 71(5): 2775-86. PubMed ID: 12704152
1245.  (INT) (TS) Wei, X. Y.; Sakr, S.; Li, J. H.; Wang, L.; Chen, W. L.; Zhang, C. C. (2006) Expression of split dnaE genes and trans-splicing of DnaE intein in the developmental cyanobacterium Anabaena sp. PCC 7120. Res Microbiol 157(3): 227-34. PubMed ID: 16256311
1246.  (INT) (REV) Weiss, M. S.; Jabs, A.; Hilgenfeld, R. (1998) Peptide bonds revisited. Nat Struct Biol 5(8): 676. PubMed ID: 9699627
1247.  (APP) (PUR) (REL) Welker, E.; Scheraga, H. A. (1999) Use of benzyl mercaptan for direct preparation of long polypeptide benzylthio esters as substrates of subtiligase. Biochem Biophys Res Commun 254(1): 147-51. PubMed ID: 9920748
1248.  (INT) Welsh, E. A.; Liberton, M.; Stockel, J.; Loh, T.; Elvitigala, T.; Wang, C.; Wollam, A.; Fulton, R. S.; Clifton, S. W.; Jacobs, J. M.; Aurora, R.; Ghosh, B. K.; Sherman, L. A.; Smith, R. D.; Wilson, R. K.; Pakrasi, H. B. (2008) The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle. Proc Natl Acad Sci U S A 105(39): 15094-9. PubMed ID: 18812508
1249.  (EN) (INT) Wende, W.; Grindl, W.; Christ, F.; Pingoud, A.; Pingoud, V. (1996) Binding, bending and cleavage of DNA substrates by the homing endonuclease Pl-SceI. Nucleic Acids Res 24(21): 4123-32. PubMed ID: 8932361
1250.  (EN) Wende, W.; SchoÃàttler, S.; Grindl, W.; Christ, F.; Steuer, S.; NoeÃÅl, A. J.; Pingoud, V.; Pingoud, A. (2000) A mutational analysis of DNA binding and cleavage by the homing endonuclease PI-SceI. Molecular Biology 34(6): 902-912.
1251.  (INT) (Structure) Werner, E.; Wende, W.; Pingoud, A.; Heinemann, U. (2002) High resolution crystal structure of domain I of the Saccharomyces cerevisiae homing endonuclease PI-SceI. Nucleic Acids Res 30(18): 3962-71. PubMed ID: 12235380
1252.  (INT) Weynberg, K. D.; Allen, M. J.; Ashelford, K.; Scanlan, D. J.; Wilson, W. H. (2009) From small hosts come big viruses: the complete genome of a second Ostreococcus tauri virus, OtV-1. Environ Microbiol 11(11): 2821-39. PubMed ID: 19650882
1253.  (INT) White, O.; Eisen, J. A.; Heidelberg, J. F.; Hickey, E. K.; Peterson, J. D.; Dodson, R. J.; Haft, D. H.; Gwinn, M. L.; Nelson, W. C.; Richardson, D. L.; Moffat, K. S.; Qin, H.; Jiang, L.; Pamphile, W.; Crosby, M.; Shen, M.; Vamathevan, J. J.; Lam, P.; McDonald, L.; Utterback, T.; Zalewski, C.; Makarova, K. S.; Aravind, L.; Daly, M. J.; Minton, K. W.; Fleischmann, R. D.; Ketchum, K. A.; Nelson, K. E.; Salzberg, S.; Smith, H. O.; Venter, J. C.; Fraser, C. M. (1999) Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1. Science. 286(5444): 1571-7. PubMed ID: 10567266
1254.  (INT) White, O. (2004) Erratum: Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1 (Science (November 19, 1999) (1571)). Science 303(5659): 766.
1255.  (APP) (REV) Wickelgren, I. (2003) Molecular biology. Spinning junk into gold. Science 300(5626): 1646-9. PubMed ID: 12805516
1256.  (APP) (NMR) (REV) (Structure) Wider, G.; Wuthrich, K. (1999) NMR spectroscopy of large molecules and multimolecular assemblies in solution. Curr Opin Struct Biol. 9(5): 594-601. PubMed ID: 10508768
1257.  (APP) (PUR) Wiese, A.; Wilms, B.; Syldatk, C.; Mattes, R.; Altenbuchner, J. (2001) Cloning, nucleotide sequence and expression of a hydantoinase and carbamoylase gene from Arthrobacter aurescens DSM 3745 in Escherichia coli and comparison with the corresponding genes from Arthrobacter aurescens DSM 3747. Appl Microbiol Biotechnol 55(6): 750-7. PubMed ID: 11525624
1258.  (REL) Wilce, J. A.; Love, S. G.; Richardson, S. J.; Alewood, P. F.; Craik, D. J. (2001) Synthesis of an analog of the thyroid hormone-binding protein transthyretin via regioselective chemical ligation. J Biol Chem. 276(28): 25997-6003. Epub 2001 May 14. PubMed ID: 11352912
1259.  (APP) (EPL) (REV) Wilken, J.; Kent, S. B. (1998) Chemical protein synthesis. Curr Opin Biotechnol. 9(4): 412-26. PubMed ID: 9720266
1260.  (APP) (EPL) (REV) Wilkinson, K. D.; Gan-Erdene, T.; Kolli, N. (2005) Derivitization of the C-Terminus of Ubiquitin and Ubiquitin-like Proteins Using Intein Chemistry: Methods and Uses. Methods Enzymol 399: 37-51. PubMed ID: 16338347
1261.  (APP) (PUR) Williams, L. R.; Ellis, S. R.; Hopper, A. K.; Davis, E. O.; Martin, N. C. (2000) Splicing before import - an intein in a mitochondrially targeted preprotein folds and is catalytically active in the cytoplasm in vivo. FEBS Lett 476(3): 301-5. PubMed ID: 10913632
1262.  (APP) (CYCL) Williams, N. K.; Prosselkov, P.; Liepinsh, E.; Line, I.; Sharipo, A.; Littler, D. R.; Curmi, P. M.; Otting, G.; Dixon, N. E. (2002) In vivo protein cyclization promoted by a circularly permuted Synechocystis sp. PCC6803 DnaB mini-intein. J Biol Chem 277(10): 7790-8. PubMed ID: 11742000
1263.  (APP) (CYCL) Williams, N. K.; Liepinsh, E.; Watt, S. J.; Prosselkov, P.; Matthews, J. M.; Attard, P.; Beck, J. L.; Dixon, N. E.; Otting, G. (2005) Stabilization of native protein fold by intein-mediated covalent cyclization. J Mol Biol 346(4): 1095-108. PubMed ID: 15701520
1264.  (APP) (NMR) (PUR) (Structure) Wojciak, J. M.; Sarkar, D.; Landy, A.; Clubb, R. T. (2002) Arm-site binding by lambda -integrase: solution structure and functional characterization of its amino-terminal domain. Proc Natl Acad Sci U S A 99(6): 3434-9. PubMed ID: 11904406
1265.  (APP) (PUR) Wojtaszek, J.; Kolaczkowska, A.; Kowalska, J.; Nowak, K.; Wilusz, T. (2006) LTCI, a novel chymotrypsin inhibitor of the potato I family from the earthworm Lumbricus terrestris. Purification, cDNA cloning, and expression. Comp Biochem Physiol B Biochem Mol Biol 143(4): 465-472. PubMed ID: 16469515
1266.  (APP) (EPL) (TAG) Wollschlager, K.; Gaus, K.; Kornig, A.; Eckel, R.; Wilking, S. D.; McIntosh, M.; Majer, Z.; Becker, A.; Ros, R.; Anselmetti, D.; Sewald, N. (2009) Single-Molecule Experiments to Elucidate the Minimal Requirement for DNA Recognition by Transcription Factor Epitopes. Small 5(4): 484-495. PubMed ID: 19199332
1267.  (APP) (PUR) Wood, D. W.; Wu, W.; Belfort, G.; Derbyshire, V.; Belfort, M. (1999) A genetic system yields self-cleaving inteins for bioseparations. Nat Biotechnol 17(9): 889-92. PubMed ID: 10471931
1268.  (APP) (PUR) Wood, D. W.; Derbyshire, V.; Wu, W.; Chartrain, M.; Belfort, M.; Belfort, G. (2000) Optimized single-step affinity purification with a self-cleaving intein applied to human acidic fibroblast growth factor. Biotechnol Prog 16(6): 1055-63. PubMed ID: 11101334
1269.  (APP) (PUR) Wood, D. W. (2003) Simplified protein purification using engineered self-cleaving affinity tags. Journal of Chemical Technology and Biotechnology 78(2-3): 103-110.
1270.  (APP) (EPL) (TAG) Wood, R. J.; Pascoe, D. D.; Brown, Z. K.; Medlicott, E. M.; Kriek, M.; Neylon, C.; Roach, P. L. (2004) Optimized conjugation of a fluorescent label to proteins via intein-mediated activation and ligation. Bioconjug Chem 15(2): 366-72. PubMed ID: 15025533
1271.  (APP) (PUR) (REV) Wood, D.W.; Skretas, G. (2005) Intein Reporter and Selection Systems. In: Homing Endonucleases and Inteins, ed. Belfort, M.; Derbyshire, V.; Stoddard, B.L.; Wood, D.W.. (Springer-Verlag, Heidelberg, Germany) Vol. 16 pages 325-344.
1272.  (APP) (REV) Wood, D.W.; Harcum, S.W.; Belfort, G. (2005B) Industrial Applications of Intein Technology. In: Homing Endonucleases and Inteins, ed. Belfort, M.; Derbyshire, V.; Stoddard, B.L.; Wood, D.W.. (Springer-Verlag, Heidelberg, Germany) Vol. 16 pages 345-364.
1273.  (REL) (REV) Woods, A. C.; Guillemette, J. G.; Parrish, J. C.; Smith, M.; Wallace, C. J. (1996) Synergy in protein engineering. Mutagenic manipulation of protein structure to simplify semisynthesis. J Biol Chem 271(50): 32008-15. PubMed ID: 8943249
1274.  (INT) (TS) Wu, H.; Hu, Z.; Liu, X. Q. (1998) Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. Proc Natl Acad Sci U S A 95(16): 9226-31. PubMed ID: 9689062
1275.  (INT) (TS) Wu, H.; Xu, M. Q.; Liu, X. Q. (1998B) Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein. Biochim Biophys Acta 1387(1-2): 422-32. PubMed ID: 9748659
1276.  (APP) (PUR) Wu, C.; Seitz, P. K.; Falzon, M. (2000) Single-column purification and bio-characterization of recombinant human parathyroid hormone-related protein (1-139). Mol Cell Endocrinol 170(1-2): 163-74. PubMed ID: 11162900
1277.  (APP) (EPL) (PUR) (Structure) Wu, J. W.; Hu, M.; Chai, J.; Seoane, J.; Huse, M.; Li, C.; Rigotti, D. J.; Kyin, S.; Muir, T. W.; Fairman, R.; Massague, J.; Shi, Y. (2001) Crystal structure of a phosphorylated Smad2. Recognition of phosphoserine by the MH2 domain and insights on Smad function in TGF-beta signaling. Mol Cell 8(6): 1277-89. PubMed ID: 11779503
1278.  (APP) (PUR) Wu, Y. Q.; Hohn, B.; Ziemienowic, A. (2001) Characterization of an ATP-dependent type I DNA ligase from Arabidopsis thaliana. Plant Mol Biol 46(2): 161-70. PubMed ID: 11442056
1279.  (APP) (PUR) Wu, W.; Wood, D. W.; Belfort, G.; Derbyshire, V.; Belfort, M. (2002) Intein-mediated purification of cytotoxic endonuclease I-TevI by insertional inactivation and pH-controllable splicing. Nucleic Acids Res 30(22): 4864-71. PubMed ID: 12433989
1280.  (APP) (PUR) Wu, X.; Oppermann, U. (2003) High-level expression and rapid purification of rare-codon genes from hyperthermophilic archaea by the GST gene fusion system. J Chromatogr B Analyt Technol Biomed Life Sci 786(1-2): 177-85. PubMed ID: 12651013
1281.  (APP) (PUR) Wu, W. Y.; Mee, C.; Califano, F.; Banki, R.; Wood, D. W. (2006) Recombinant protein purification by self-cleaving aggregation tag. Nat Protoc 1(5): 2257-62. PubMed ID: 17406465
1282.  (APP) (EPL) (TAG) Wu, Y. W.; Tan, K. T.; Waldmann, H.; Goody, R. S.; Alexandrov, K. (2007) Interaction analysis of prenylated Rab GTPase with Rab escort protein and GDP dissociation inhibitor explains the need for both regulators. Proc Natl Acad Sci U S A 104(30): 12294-9. PubMed ID: 17640890
1283.  (APP) (PUR) (REV) Wu, W. Y.; Fong, B. A.; Gilles, A. G.; Wood, D. W. (2009) Recombinant protein purification by self-cleaving elastin-like polypeptide fusion tag. Curr Protoc Protein Sci Chapter 26: Unit 26 4 1-18. PubMed ID: 19937722
1284.  (APP) (EPL) (TAG) Wu, Y. W.; Oesterlin, L. K.; Tan, K. T.; Waldmann, H.; Alexandrov, K.; Goody, R. S. (2010) Membrane targeting mechanism of Rab GTPases elucidated by semisynthetic protein probes. Nat Chem Biol 6(7): 534-40. PubMed ID: 20512138
1285.  (APP) (PUR) Wu, W. Y.; Gillies, A. R.; Hsii, J. F.; Contreras, L.; Oak, S.; Perl, M. B.; Wood, D. W. (2010B) Self-cleaving purification tags re-engineered for rapid Topo(R) cloning. Biotechnol Prog 26(5): 1205-12. PubMed ID: 20945478
1286.  (APP) (PUR) Wuebbens, M. M.; Rajagopalan, K. V. (2003) Mechanistic and mutational studies of Escherichia coli molybdopterin synthase clarify the final step of molybdopterin biosynthesis. J Biol Chem 278(16): 14523-32. PubMed ID: 12571226
1287.  (APP) (EPL) (TAG) (TS) Xia, Z.; Xing, Y.; So, M. K.; Koh, A. L.; Sinclair, R.; Rao, J. (2008) Multiplex detection of protease activity with quantum dot nanosensors prepared by intein-mediated specific bioconjugation. Anal Chem 80(22): 8649-55. PubMed ID: 18922019
1288.  (APP) (TS) Xiao, X.; Li, J.; Sun, W.; Wang, B.; Liu, X. Q. (2008) Protein trans-splicing as a means for viral vector-mediated in vivo gene therapy. Hum Gene Ther Jun 17. [Epub ahead of print] [see Li 2008]. PubMed ID: 18558811
1289.  (APP) (INT) (PUR) (REV) Xie, J.; Liu, C. Q.; Huang, J. F.; Shi, X. F.; Shao, D. (2001) Recent advance in the study of intein. Progress in Biochemistry and Biophysics 28(2): 186-187.
1290.  (INT) Xie, J.; Juang, J.F.; Shi, X.F.; Liu, C.Q. (2001) Analysis of the characteristic sequence of intein and revision of its motifs. Chinese Sci Bull 46: 758-761.
1291.  (APP) (PUR) Xie, L.; Chatterjee, C.; Balsara, R.; Okeley, N. M.; van der Donk, W. A. (2002) Heterologous expression and purification of SpaB involved in subtilin biosynthesis. Biochem Biophys Res Commun 295(4): 952-7. PubMed ID: 12127987
1292.  (APP) (REL) Xie, L.; Miller, L. M.; Chatterjee, C.; Averin, O.; Kelleher, N. L.; van der Donk, W. A. (2004) Lacticin 481: in vitro reconstitution of lantibiotic synthetase activity. Science. 303(5658): 679-81. PubMed ID: 14752162
1293.  (APP) (EPL) (TAG) Xie, N.; Elangwe, E. N.; Asher, S.; Zheng, Y. G. (2009) A dual-mode fluorescence strategy for screening HAT modulators. Bioconjug Chem 20(2): 360-6. PubMed ID: 19146394
1294.  (INT) (NEB) Xu, M. Q.; Southworth, M. W.; Mersha, F. B.; Hornstra, L. J.; Perler, F. B. (1993) In vitro protein splicing of purified precursor and the identification of a branched intermediate. Cell 75(7): 1371-7. PubMed ID: 8269515
1295.  (INT) (NEB) Xu, M. Q.; Comb, D. G.; Paulus, H.; Noren, C. J.; Shao, Y.; Perler, F. B. (1994) Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation. Embo J 13(23): 5517-22. PubMed ID: 7988548
1296.  (INT) (NEB) Xu, M. Q.; Perler, F. B. (1996) The mechanism of protein splicing and its modulation by mutation. Embo J 15(19): 5146-53. PubMed ID: 8895558
1297.  (APP) (NEB) (PUR) (REV) Xu, M.-Q. (1997) The IMPACT of protein splicing research. The NEB Transcript 8(2): 1-5.
1298.  (APP) (EPL) (NMR) (PUR) (Structure) Xu, R.; Ayers, B.; Cowburn, D.; Muir, T. W. (1999) Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies. Proc Natl Acad Sci U S A. 96(2): 388-93. PubMed ID: 9892643
1299.  (NEB) (REL) (REV) Xu, Q.; Buckley, D.; Guan, C.; Guo, H. C. (1999B) Structural insights into the mechanism of intramolecular proteolysis. Cell. 98(5): 651-61. PubMed ID: 10490104
1300.  (APP) (NEB) (PUR) (REV) Xu, M. Q.; Paulus, H.; Chong, S. (2000) Fusions to self-splicing inteins for protein purification. Methods Enzymol 326: 376-418. PubMed ID: 11036654
1301.  (APP) (EPL) (NEB) (REV) Xu, M. Q.; Evans, T. C., Jr. (2001) Intein-mediated ligation and cyclization of expressed proteins. Methods 24(3): 257-77. PubMed ID: 11403575
1302.  (APP) (NEB) (PUR) (REV) Xu, M. Q.; Evans, T. C., Jr. (2003) Purification of recombinant proteins from E. coli by engineered inteins. Methods Mol Biol 205: 43-68. PubMed ID: 12491879
1303.  (APP) (EPL) (NEB) (TAG) Xu, J.; Sun, L.; Ghosh, I.; Xu, M. Q. (2004) Western blot analysis of Src kinase assays using peptide substrates ligated to a carrier protein. Biotechniques 36(6): 976-8, 980-1. PubMed ID: 15211748
1304.  (APP) (NEB) (REV) Xu, M. Q.; Evans, T. C., Jr. (2005) Recent advances in protein splicing: manipulating proteins in vitro and in vivo. Curr Opin Biotechnol 16(4): 440-6. PubMed ID: 16026977
1305.  (APP) (EPL) (NEB) (TAG) Xu, M. Q.; Ghosh, I.; Kochinyan, S.; Sun, L. (2007) Intein-mediated peptide arrays for epitope mapping and kinase/phosphatase assays. Methods Mol Biol 381: 313-38. PubMed ID: 17984527
1306.  (APP) (PUR) Xu, C. G.; Fan, X. J.; Zhang, Z. Y.; Fu, Y. J.; Liang, A. H. (2007) [Soluble expression, purification and characterization of Bm K IT in Escherichia coli by intein-mediated system]. Sheng Wu Gong Cheng Xue Bao 23(6): 989-94. PubMed ID: 18257224
1307.  (APP) (PUR) Xu, C. G.; Fan, X. J.; Fu, Y. J.; Liang, A. H. (2008) Effect of location of the His-tag on the production of soluble and functional Buthus martensii Karsch insect toxin. Protein Expr Purif 59(1): 103-9. PubMed ID: 18289876
1308.  (APP) (NMR) (PUR) (Structure) (TS) Yagi, H.; Tsujimoto, T.; Yamazaki, T.; Yoshida, M.; Akutsu, H. (2004) Conformational change of H+-ATPase beta monomer revealed on segmental isotope labeling NMR spectroscopy. J Am Chem Soc 126(50): 16632-8. PubMed ID: 15600369
1309.  (INT) Yamamoto, K.; Low, B.; Rutherford, S. A.; Rajagopalan, M.; Madiraju, M. V. (2001) The Mycobacterium avium-intracellulare complex dnaB locus and protein intein splicing. Biochem Biophys Res Commun 280(3): 898-903. PubMed ID: 11162608
1310.  (INT) Yamamoto, K.; Rutherford, S. A.; Rajagopalan, M.; Hirakawa, H.; Kuhara, S.; Banno, Y.; Fujii, H.; Madiraju, M. V. V. S. (2004) Heterogeneity of dnaB Locus of Mycobacterium avium-intracellulare Complex. Journal of the Faculty of Agriculture, Kyushu University 49(2): 375-381.
1311.  (APP) (REL) Yamamoto, N.; Takayanagi, A.; Yoshino, A.; Sakakibara, T.; Kajihara, Y. (2007) An approach for a synthesis of asparagine-linked sialylglycopeptides having intact and homogeneous complex-type undecadisialyloligosaccharides. Chemistry 13(2): 613-25. PubMed ID: 16977655
1312.  (APP) (NMR) (Structure) (TS) Yamazaki, T.; Otomo, T.; Oda, N.; Kyogoku, Y.; Uegaki, K.; Ito, N.; Ishino, Y.; Nakamura, H. (1998) Segmental isotope labeling for protein NMR using peptide splicing. J. Am. Chem. Soc. 120: 5591-5592.
1313.  (APP) (PUR) Yan, S. S.; Yan, J.; Shi, G.; Xu, Q.; Chen, S. C.; Tian, Y. W. (2005) Production of native protein by using Synechocystis sp. PCC6803 DnaB mini-intein in Escherichia coli. Protein Expr Purif 40(2): 340-5. PubMed ID: 15766876
1314.  (APP) (TS) Yang, J.; Fox, G. C., Jr.; Henry-Smith, T. V. (2003) Intein-mediated assembly of a functional beta-glucuronidase in transgenic plants. Proc Natl Acad Sci U S A 100(6): 3513-8. PubMed ID: 12629210
1315.  (INT) Yang, J.; Meng, Q.; Liu, X. Q. (2004) Intein harbouring large tandem repeats in replicative DNA helicase of Trichodesmium erythraeum. Mol Microbiol 51(4): 1185-92. PubMed ID: 14763989
1316.  (APP) (PUR) (TS) Yang, J.; Henry-Smith, T. V.; Qi, M. (2006) Functional analysis of the split Synechocystis DnaE intein in plant tissues by biolistic particle bombardment. Transgenic Res 15(5): 583-93. PubMed ID: 16830226
1317.  (APP) (PUR) Yang, B.; Chen, S.; Tong, Y.; Qin, Y. (2006B) [Construction and expression of protein self-splicing prokaryotic expression vector pTWIN1- AcAPc2]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 23(3): 630-4. PubMed ID: 16856404
1318.  (APP) (TAG) (TS) Yang, J. Y.; Yang, W. Y. (2009) Site-specific two-color protein labeling for FRET studies using split inteins. J Am Chem Soc 131(33): 11644-5. PubMed ID: 19645470
1319.  (APP) (PUR) Ye, Xinhao; Rollin, Joe; Zhang, Yi-Heng Percival (2010) Thermophilic [alpha]-glucan phosphorylase from Clostridium thermocellum: Cloning, characterization and enhanced thermostability. Journal of Molecular Catalysis B: Enzymatic 65(1-4): 110-116.
1320.  (APP) (EPL) (TAG) Yee, C. S.; Seyedsayamdost, M. R.; Chang, M. C.; Nocera, D. G.; Stubbe, J. (2003) Generation of the R2 subunit of ribonucleotide reductase by intein chemistry: insertion of 3-nitrotyrosine at residue 356 as a probe of the radical initiation process. Biochemistry 42(49): 14541-52. PubMed ID: 14661967
1321.  (APP) (EPL) Yee, C. S.; Chang, M. C.; Ge, J.; Nocera, D. G.; Stubbe, J. (2003) 2,3-difluorotyrosine at position 356 of ribonucleotide reductase R2: a probe of long-range proton-coupled electron transfer. J Am Chem Soc 125(35): 10506-7. PubMed ID: 12940718
1322.  (APP) (EPL) (REV) Yeo, D. S.; Srinivasan, R.; Chen, G. Y.; Yao, S. Q. (2004) Expanded utility of the native chemical ligation reaction. Chemistry 10(19): 4664-72. PubMed ID: 15372685
1323.  (APP) (EPL) (REV) (TAG) Yeo, D. S. Y.; Panicker, R. C.; Tan, L. P.; Yao, S. Q. (2004) Strategies for immobilization of biomolecules in a microarray. Combinatorial Chemistry and High Throughput Screening 7(3): 213-221.
1324.  (APP) (PUR) Yi, T.; Hong, A.; Xie, S.; Zhang, L.; Xie, Q.; Dai, Y.; Yu, R. (2008) [Preparation and identification of recombinant maxadilan]. Sheng Wu Gong Cheng Xue Bao 24(12): 2049-55.
1325.  (INT) York, Daniel J.; Nadelson, Adam C.; Reitter, Julie N.; Mills, Kenneth V. (2009) Alternate protein splicing mechanisms: A directed evolution approach. FASEB J._MeetingAbstracts 23(1_MeetingAbstracts): 502.4.
1326.  (INT) York, Daniel J.; Duffee, Lauren R.; Dorval, Deirdre M.; Reitter, Julie N.; Mills, Kenneth V. (2010) Estimating the activation barrier to each step of protein splicing for the non-canonical P. abyssi PolII intein. FASEB J. 24(1_MeetingAbstracts): 463.18-.
1327.  (APP) (NMR) (REV) (Structure) Yu, H. (1999) Extending the size limit of protein nuclear magnetic resonance. Proc Natl Acad Sci U S A 96(2): 332-4. PubMed ID: 9892632
1328.  (APP) (PUR) Yu, R. J.; Hong, A.; Dai, Y.; Gao, Y. (2004) Intein-mediated rapid purification of recombinant human pituitary adenylate cyclase activating polypeptide. Acta Biochim Biophys Sin (Shanghai) 36(11): 759-66. PubMed ID: 15514850
1329.  (APP) (PUR) Yu, R. J.; Xie, Q. L.; Dai, Y.; Gao, Y.; Zhou, T. H.; Hong, A. (2006) Intein-mediated rapid purification and characterization of a novel recombinant agonist for VPAC2. Peptides 27(6): 1359-66. PubMed ID: 16500728
1330.  (APP) (PUR) Yu, R. J.; Tam, N. L.; Gao, Y.; Zeng, Z. H.; Zhou, T. H.; Hong, A. (2007) A novel recombinant, VPAC2-selective agonist enhancing insulin release and glucose disposal. Acta Pharmacol Sin 28(4): 526-33. PubMed ID: 17376292
1331.  (APP) (PUR) Yu, R.; Yi, T.; Zhang, L.; Hong, A.; Dai, Y.; Zhou, T. (2008) Intein-mediated rapid purification of recombinant maxadilan and M65 and their acute effects on plasma glucose. Acta Biochim Biophys Sin (Shanghai) 40(12): 1015-22. PubMed ID: 19089299
1332.  (APP) (EPL) Yu, H. H.; Nakase, I.; Pujals, S.; Hirose, H.; Tanaka, G.; Katayama, S.; Imanishi, M.; Futaki, S. (2010) Expressed protein ligation for the preparation of fusion proteins with cell penetrating peptides for endotoxin removal and intracellular delivery. Biochim Biophys Acta (IN PRESS). PubMed ID: 20170629
1333.  (APP) (CPS) Yuen, C. M.; Rodda, S. J.; Vokes, S. A.; McMahon, A. P.; Liu, D. R. (2006) Control of transcription factor activity and osteoblast differentiation in Mammalian cells using an evolved small-molecule-dependent intein. J Am Chem Soc 128(27): 8939-46. PubMed ID: 16819890
1334.  (APP) (CPS) Zeidler, M. P.; Tan, C.; Bellaiche, Y.; Cherry, S.; Hader, S.; Gayko, U.; Perrimon, N. (2004) Temperature-sensitive control of protein activity by conditionally splicing inteins. Nat Biotechnol 22(7): 871-6. PubMed ID: 15184905
1335.  (APP) (PUR) Zeng, L.; Yu, R.; Xu, M.; Chen, J.; Wang, J.; Li, J. (2009) [Preparation and identification of recombinant PTD-maxadilan]. Sheng Wu Gong Cheng Xue Bao 25(11): 1739-45. PubMed ID: 20222476
1336.  (EN) (REL) Zeng, Q.; Bonocora, R. P.; Shub, D. A. (2009) A free-standing homing endonuclease targets an intron insertion site in the psbA gene of cyanophages. Curr Biol 19(3): 218-22. PubMed ID: 19200728
1337.  (TS) Zettler, J.; Schutz, V.; Mootz, H. D. (2009) The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction. FEBS Lett 583(5): 909-14. PubMed ID: 19302791
1338.  (APP) (NEB) (PUR) (TAG) Zhang, A.; Gonzalez, S. M.; Cantor, E. J.; Chong, S. (2001) Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins. Gene 275(2): 241-52. PubMed ID: 11587851
1339.  (APP) (EPL) Zhang, Z.; Shen, K.; Lu, W.; Cole, P. A. (2003) The role of C-terminal tyrosine phosphorylation in the regulation of SHP-1 explored via expressed protein ligation. J Biol Chem 278(7): 4668-74. PubMed ID: 12468540
1340.  (APP) (PUR) Zhang, X. L.; Wang, J. R.; Zhang, Y.; Chen, M. L.; Zhang, W.; Yang, S.; Jiang, W. H. (2003B) [Expression, purification and identification of recombinant SARS coronavirus membrane protein]. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35(12): 1140-4. PubMed ID: 14673508
1341.  (APP) (PUR) Zhang, Y.; Calin-Jageman, I.; Gurnon, J. R.; Choi, T. J.; Adams, B.; Nicholson, A. W.; Van Etten, J. L. (2003C) Characterization of a chlorella virus PBCV-1 encoded ribonuclease III. Virology 317(1): 73-83. PubMed ID: 14675626
1342.  (APP) (PUR) Zhang, X. L.; Wang, J. R.; Zhang, Y.; Chen, M. L.; Zhang, W.; Yang, S.; Jiang, W. H. (2003C) Expression, purification and identification of recombinant SARS coronavirus membrane protein. Acta Biochimica et Biophysica Sinica 35(12): 1140-1144.
1343.  (APP) (NEB) (PUR) Zhang, Aihua; Cantor, Eric J.; Barshevsky, Tanya; Chong, Shaorong (2005) Productive interaction of chaperones with substrate protein domains allows correct folding of the downstream GFP domain. Gene 350(1): 25-31.
1344.  (INT) Zhang, L.; Zheng, Y.; Xi, Z.; Luo, Z.; Xu, X.; Wang, C.; Liu, Y. (2009) Metal ions binding to recA inteins from Mycobacterium tuberculosis. Mol Biosyst 5(6): 644-50. PubMed ID: 19462022
1345.  (APP) (EPL) (REV) Zhang, X.; van der Donk, W. A. (2009B) Using expressed protein ligation to probe the substrate specificity of lantibiotic synthetases. Methods Enzymol 462: 117-34. PubMed ID: 19632472
1346.  (CPS) (INT) Zhang, L.; Xiao, N.; Pan, Y.; Zheng, Y.; Pan, Z.; Luo, Z.; Xu, X.; Liu, Y. (2010) Binding and inhibition of copper ions to RecA inteins from Mycobacterium tuberculosis. Chemistry 16(14): 4297-306. PubMed ID: 20209535
1347.  (APP) (PUR) Zhang, S.; Wang, Z. H.; Chen, G. Q. (2010B) Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification. Microb Cell Fact 9: 28. PubMed ID: 20459707
1348.  (APP) (PUR) (TS) Zhao, J.; Gao, S. Q.; Fei, Y. B.; Wei, L. B. (2004) [Construction and expression of vector with aroA-in gene and its transformation in tobacco]. Yi Chuan Xue Bao 31(11): 1294-301. PubMed ID: 15651683
1349.  (APP) (PUR) (TS) Zhao, J.; Gao, S. Q.; Fei, Y. B.; Wei, L. B. (2004) Construction and expression of vector with aroA-In gene and its transformation in tobacco. Acta Genetica Sinica 31(11): 1294-1301.
1350.  (APP) (NEB) (PUR) Zhao, Z.; Lu, W.; Dun, B.; Jin, D.; Ping, S.; Zhang, W.; Chen, M.; Xu, M. Q.; Lin, M. (2008) Purification of green fluorescent protein using a two-intein system. Appl Microbiol Biotechnol 77(5): 1175-80. PubMed ID: 17973109
1351.  (APP) (EPL) (NMR) (PUR) (Structure) Zhao, W.; Zhang, Y.; Cui, C.; Li, Q.; Wang, J. (2008B) An efficient on-column expressed protein ligation strategy: application to segmental triple labeling of human apolipoprotein E3. Protein Sci 17(4): 736-47. PubMed ID: 18305193
1352.  (APP) (CYCL) (TS) Zhao, Z.; Ma, X.; Li, L.; Zhang, W.; Ping, S.; Xu, M. Q.; Lin, M. (2010) Protein cyclization enhanced thermostability and exopeptidase-resistance of green fluorescent protein. J Microbiol Biotechnol 20(3): 460-6. PubMed ID: 20372012
1353.  (INT) Zheng, R.; Matsui, E.; Shen, Y.; Musti, K. V.; Feng, Y.; Darnis, S.; Kawarabayasi, Y.; Kikuchi, H.; Harata, K.; Matsui, I. (2001) The novel function of a short region K253xRxxxD259 conserved in the exonuclease domain of hyperthermostable DNA polymerase I from Pyrococcus horikoshii. Extremophiles 5(2): 111-7. PubMed ID: 11354454
1354.  (APP) (EPL) Zheng, W.; Schwarzer, D.; Lebeau, A.; Weller, J. L.; Klein, D. C.; Cole, P. A. (2005) Cellular stability of serotonin N-acetyltransferase conferred by phosphonodifluoromethylene alanine (Pfa) substitution for Ser-205. J Biol Chem 280(11): 10462-7. PubMed ID: 15632116
1355.  (APP) (PUR) Zhgun, A. A.; El'darov, M. A.; Solodar, L. I.; Sokolov, N. N.; Archakov, A. I.; Skriabin, K. G. (2001) [Heterologous expression of eukaryotic CYP450. 1. Heterologous expression of cytochrome P450 2B4 using groups with various affinity in E. coli]. Vopr Med Khim 47(4): 382-92. PubMed ID: 11693026
1356.  (APP) (PUR) Zhong, Q.; Lazar, C. S.; Tronchere, H.; Sato, T.; Meerloo, T.; Yeo, M.; Songyang, Z.; Emr, S. D.; Gill, G. N. (2002) Endosomal localization and function of sorting nexin 1. Proc Natl Acad Sci U S A 99(10): 6767-72. PubMed ID: 11997453
1357.  (APP) (CYCL) (TS) Zhonglin, Z.; Ma, X.; Li, L.; Zhang, W.; Ping, S.; Xu, M. Q.; Lin, M. (2010) Protein cyclization enhanced thermostability and exopeptidase resistance of green fluorescent protein. Journal of Microbiology and Biotechnology 20(3): 460-466.
1358.  (APP) (PUR) Zhou, H.; Yan, F.; Tai, H. H. (2001) C-Terminal region of human NAD+-dependent 15-hydroxyprostaglandin dehydrogenase is involved in the interaction with prostaglandin substrates. Eur J Biochem 268(12): 3368-74. PubMed ID: 11422365
1359.  (APP) (PUR) Zhou, X.; Song, Z.; Liu, X.; Jia, F.; Wang, Y. (2010) Production of Recombinant Porcine Interferon alpha Using PHB-Intein-Mediated Protein Purification Strategy. Appl Biochem Biotechnol (IN PRESS). PubMed ID: 20957450
1360.  (INT) Zhu, W.; Reich, C. I.; Olsen, G. J.; Giometti, C. S.; Yates, J. R., 3rd (2004) Shotgun proteomics of Methanococcus jannaschii and insights into methanogenesis. J Proteome Res 3(3): 538-48. PubMed ID: 15253435
1361.  (APP) (PUR) (TS) Zhu, F.; Miao, J.; Qu, H.; Chi, X. (2009) [Separate expression of ABCA1 and ligation mediated by Ssp DnaE intein in Escherichia coli BL21 (DE3)]. Wei Sheng Wu Xue Bao 49(12): 1601-6. PubMed ID: 20222445
1362.  (APP) (PUR) (TS) Zhu, F.; Liu, Z.; Qu, H.; Xin, X.; Dong, H.; Liu, X. (2009B) [Ssp DnaB intein-mediated ligation of heavy and light chains of coagulation factor VIII in Escherichia coli]. Sheng Wu Gong Cheng Xue Bao 25(7): 1101-6. PubMed ID: 19835155
1363.  (APP) (PUR) (TS) Zhu, F. X.; Liu, Z. L.; Qu, H. G.; Chi, X. Y. (2009C) [Intein-mediated F309SfVIII ligation with enhanced secretion of its heavy chain.]. Sheng Li Xue Bao 61(6): 526-32. PubMed ID: 20029685
1364.  (APP) (PUR) (TS) Zhu, F.; Liu, Z.; Chi, X.; Qu, H. (2010) Protein trans-splicing based dual-vector delivery of the coagulation factor VIII gene. Sci China Life Sci 53(6): 683-9. PubMed ID: 20602271
1365.  (APP) (PUR) (TS) Zhu, F. X.; Yang, S. D.; Liu, Z. L.; Miao, J.; Qu, H. G.; Chi, X. Y. (2010B) vWF improves secretion and activity of intein spliced BDD-FVIII. Yaoxue Xuebao 45(5): 595-600.
1366.  (APP) (PUR) (TS) Zhu, F. X.; Liu, Z. L.; Qu, H. G.; Chi, X. Y. (2010C) Post-translational ligation and function of dual-vector transferred split CFTR gene. Yaoxue Xuebao 45(1): 60-65.
1367.  (APP) (TS) Zhu, F. X.; Liu, Z. L.; Qu, H. G.; Chi, X. Y. (2010D) [Enhancing effect of porcine coagulation factor VIII A1 and A3 domains on secretion of post-translationally spliced human/porcine hybrid coagulation factor VIII.]. Sheng Li Xue Bao 62(4): 373-81. PubMed ID: 20717639
1368.  (APP) (EPL) (REL) Ziaco, B.; Pensato, S.; D'Andrea, L. D.; Benedetti, E.; Romanelli, A. (2008) Semisynthesis of dimeric proteins by expressed protein ligation. Org Lett 10(10): 1955-8. PubMed ID: 18410123
1369.  (APP) (EPL) Zuberek, J.; Wyslouch-Cieszynska, A.; Niedzwiecka, A.; Dadlez, M.; Stepinski, J.; Augustyniak, W.; Gingras, A. C.; Zhang, Z.; Burley, S. K.; Sonenberg, N.; Stolarski, R.; Darzynkiewicz, E. (2003) Phosphorylation of eIF4E attenuates its interaction with mRNA 5' cap analogs by electrostatic repulsion: intein-mediated protein ligation strategy to obtain phosphorylated protein. RNA 9(1): 52-61. PubMed ID: 12554876
1370.  (APP) (NMR) (Structure) (TS) Zuger, S.; Iwai, H. (2005) Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies. Nat Biotechnol 23(6): 736-40. PubMed ID: 15908942
1371.  (APP) (PUR) Zzaman, S.; Abhyankar, M. M.; Bastia, D. (2004) Reconstitution of F factor DNA replication in vitro with purified proteins. J Biol Chem 279(17): 17404-10. PubMed ID: 14973139

Bibliography updated: 11/05/10
Last database update: 11/05/10

InBase Home Background Info Splicing mechanism Splicing motifs DOD Endo motifs
Intein registry Intein alleles Selected properties  
Do you have an intein? Submitting data Bibliography Intein links NEB Home