| Intein Name |
Pfu RtcB (Pfu Hyp-2) |
| Prototype Allele |
Mja RtcB |
| Extein Name |
RNA terminal phosphate cyclase operon orfB |
| Intein Class |
Theoretical |
| Organism Name |
Pyrococcus furiosus |
| Organism Description |
Thermophile, taxon:186497, DSM3638 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
N97 |
| Insert Site Comments |
rtcB-a |
| Intein Size (aa) |
380 |
| Intein N-terminal |
SPGGIGYDIN/C |
| Intein C-terminal |
HN/CGVRLIRTNL |
| Accession No. |
AAL81739.1 in NCBI/protein, PF1615 |
| Intein aa Sequence |
>gi|18893765|gb|AAL81739.1|[18893765] Pfu RtcB (Pfu Hyp-2) intein (including -1 and +1 extein residues)
NCLAPGTKVLTEHGYWLKIEEMPEKFKLQRLRLYNIEEGHNDFSRVAFVAERNIEKDETA
IRIVTETGTLIEGSEDHPVLTPQGYVYLKNIKEGDYVIVYPFEGVPYEEKKGIIIDESAF
EGEDPQvIKFLKERNLLPLRWEDPKIGTLARILGFALGDGHLGEMGGRLVLAFYGREETL
RELKKDLESLGIKANLYVREKNYRIKTESGEYSGKTVLAELRVSSRSFALLLEKLGMPRG
EKTKKAYRIPVWIMEAPLWVKRNFLAGFFGADGSIVEFKGTTPLPIHLTQAKDVALEENL
KEFLYDISRILEEFGVKTTIYKVNSKKSVTYRLSIVGEENIRNFLGKINYEYDPKKKAKG
LIAYAYLKFKESVKKERRKAMEISKKIYEETGNIDRAYKAVKDIVNRRFVERTIYEGERN
PRVPKNFLTFEEFAKERGYEGGFVAEKVVKVERIKPEYDRFYDIGVYHEAHNFIANGIVV
HNC |
| Block A |
CLAPGTKVLTEHG 13 |
| Block B |
GTLIEGSEDHPVLT 80 |
| Block C |
ILGFALGDG 159 |
| Block D |
AYRIPVWI 252 |
| Block E |
FLAGFFGADG 272 |
| Block H |
ENIKEFLYDISRILEEFGV 314 |
| Block F |
RFYDIGV*YHEAHNFI 473 |
| Block G |
NGIVVHNC 482 |
| Initially Contributed by |
Dennis Maeder |
| Contributor's Address |
COMB
701 E.Pratt St.
Baltimore MD 21202 |
| Contributor's Phone No. |
410-234-8871 |
| Contributor's FAX No. |
410-234-8896 |
| Contributor's Email address |
maeder@umbi.umd.edu |
| Independently Found By |
|
| Comments |
Formally called Hyp-2 intein. In E.coli this gene is in an operon with rtcA - an RNA 3'-terminal phosphate cyclase - and rtcR the sigma-54 regulator of rtcA and rtcB (Pietrokovski, Genschik 98). |
| Date Submitted |
08/28/1998 |
| References |
Maeder 1999
Genschik 1997
Genschik 1998 |
