| Intein Name |
Asp DnaE-n |
| Prototype Allele |
Ssp DnaE-n |
| Extein Name |
DnaE pol subunit, DNA polymerase III alpha subunit |
| Intein Class |
Experimental |
| Organism Name |
Anabaena species PCC7120, (Nostoc sp. PCC7120) |
| Organism Description |
Cyanobacterium, Nitrogen-fixing, taxon:103690 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
None |
| Location in Extein |
Y775 |
| Insert Site Comments |
dnaE-a, Beta and tau binding domains |
| Intein Size (aa) |
102 |
| Intein N-terminal |
FDDMLKFAEY/C |
| Intein C-terminal |
SN/CFNKSHSTAY |
| Accession No. |
NP_487618.1 in NCBI/protein, all3578 |
| Intein aa Sequence |
>gi|17231070|ref|NP_487618.1|[17231070] Asp DnaE-n N-terminal intein fragment (including -1 extein residue)
YCLSYDTEVLTVEYGFVPIGEIVEKGIECSVFSINNNGIVYTQPIAQWHH
RGKQEVFEYCLEDGSIIKATKDHKFMTQDGKMLPIDEIFEQELDLLQVKG
LPE |
| Block A |
CLSYDTEVLTVEY N13 |
| Block B |
GSIIKATKDHKFMT N76 |
| Block C |
None |
| Block D |
None |
| Block E |
None |
| Block H |
None |
| Block F |
|
| Block G |
|
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
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| Comments |
This DnaE intein is a natuarlly split intein. Homolog of the catalytic alpha subunit of E. coli DNA polymerase III. BLOCKS A & B ARE IN Fragment N AND BLOCKS F & G ARE IN Fragment C. |
| Date Submitted |
07/07/2002 |
| References |
Kaneko 2001
Caspi 2003
Wei 2006 |
