| Intein Name |
Chy RIR1 |
| Prototype Allele |
Pfu RIR1-2 |
| Extein Name |
Ribonucleoside- diphosphate reductase, alpha subunit |
| Intein Class |
Experimental |
| Organism Name |
Carboxydothermus hydrogenoformans Z-2901 |
| Organism Description |
Thermophile, taxon=246194 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
P239 |
| Insert Site Comments |
RIR1-b |
| Intein Size (aa) |
345 |
| Intein N-terminal |
ELGEIESTNP/C |
| Intein C-terminal |
LD/CGEQPLLPYE |
| Accession No. |
YP_359531 in NCBI/protein, CHY_0676, gene="nrdJ |
| Intein aa Sequence |
>gi|78044854|ref|YP_359531.1|[78044854]| Chy RIR1 intein (including -1 and +1 extein residues)
PCVTGDTLVFTDKGLIEARKLEVGMKVWSGDGWNEIKEVINNGVKPVLKLKLKTGLEIKVTEEHKIFTGEGWKEAKDLKV
GDKLYLPVSYPELDFPVKEENDFYEFLGYFLGDGSLSVSNHVSLHVGNDKELALYFKEKVEKYAGAAYLIERDGQYIIDV
HRKEFAEKIKKIFGIEITDSKEKDIPSSLLAVNSEAMKALLRGLFSADGSVYDANGSITVALSSTSYPLLRKVQILLLSL
GIPSTLTGEKDQDVKIIKGNEYETLPTYRLIISGERASLFFNKIGLIGEKKKKFLELMAGKTTYSTLNNHLYQEIVSIEP
AGEEEVFDITAPPKYTWITNGILSLDC |
| Block A |
CVTGDTLVFTDKG 13 |
| Block B |
GLEIKVTEEHKIFT 67 |
| Block C |
FLGYFLGDG 113 |
| Block D |
EKDIPSSL 188 |
| Block E |
LLRGLFSADG 208 |
| Block H |
STSYPLLRKVQILLLSLGI 241 |
| Block F |
EVFDITA**PPKYTWI 337 |
| Block G |
NGILSLDC 346 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
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| Comments |
First intein with +1 Asp. New splicing mechanism proposed. |
| Date Submitted |
03/23/2004 |
| References |
Amitai 2004 |
