| Intein Name |
CroV Top2 |
| Prototype Allele |
|
| Extein Name |
DNA Topoisomerase IIA |
| Intein Class |
Theoretical |
| Organism Name |
Cafeteria roenbergensis virus BV-PW1 |
| Organism Description |
taxon:693272, Giant virus infecting marine heterotrophic nanoflagellate |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
K140 |
| Insert Site Comments |
Top2-a |
| Intein Size (aa) |
387 |
| Intein N-terminal |
GGMNGLGSK/C |
| Intein C-terminal |
HN/CSNVWSKKFE |
| Accession No. |
ADO67359 in NCBI/protein,locus_tag="crov325 |
| Intein aa Sequence |
>ADO67359.1 GI:309386499 | CroV Top2 intein (including -1 and +1 extein residues)
KCISSDTNVLIWNSKISKKAKDIQIGDILVGDDGNKRNVIDVSFGKGQMYKIIQSKGENYSVNNNHTLTLMMPLHKVISN
SNNKLKLLWWDNVYKIIKSKPIDVQENKISDDEILLNSVYHDRTKHYRKVRKESQIYTLAEIDQLNLSDEIKKEIIDLCN
TIPDENVFDINIQDYMNLDKTTQTYLIGVSGKCINYPYKSVNIDPYLLGLWIGEDCSHNSKIYLNNRVNLNMYKLKSKGI
PDDYLYNNKYIRESVLAGIIDKKGSVVRNGTKIIIEQKLSNKELINDIIYLAQSLGFVCSTANINKTITTTGVTHSDNIK
TIYITGNLASIPVLREKNKCINPTNFNILDSPGYITVEKDGIGDYVSITVDTTTNQRFLINDFTVTHNC |
| Block A |
CISSDTNVLIWNS 13 |
| Block B |
GENYSVNNNHTLTL 69 |
| Block C |
LLGLWIGED 214 |
| Block D |
SKGIPDDY 243 |
| Block E |
VLAGIIDKKG 263 |
| Block H |
LSNKELINDIIYLAQSLGF 296 |
| Block F |
DYVSITVDTTTNQRFL 378 |
| Block G |
DFTVTHNC 388 |
| Initially Contributed by |
Matthias G. Fischer |
| Contributor's Address |
University of British Columbia
6339 Stores Road
Vancouver, BC, Canada
V6T 1Z4 |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
mfischer@eos.ubc.ca |
| Independently Found By |
|
| Comments |
The positioning of Endo Block C relative to other blocks is very different than in other intein DOD endos (FP). |
| Date Submitted |
11/24/2010 |
| References |
Fischer 2010 |
