| Intein Name |
Sce-OUT7112 VMA |
| Prototype Allele |
Sce VMA |
| Extein Name |
Vacuolar ATPase (H+-transporting ATP synthase), subunit A |
| Intein Class |
Theoretical |
| Organism Name |
Saccharomyces cerevisiae OUT7112 |
| Organism Description |
Yeast,taxon:4932, also in Sce strains OUT7900, OUT7903 |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
G258 |
| Insert Site Comments |
VMA-a |
| Intein Size (aa) |
454 |
| Intein N-terminal |
SNSDAIIYVG/C |
| Intein C-terminal |
HN/CGERGNEMAE |
| Accession No. |
BAC66643 in NCBI/protein |
| Intein aa Sequence |
>gi|29420843|dbj|BAC66643.1|[29420843] Sce-OUT7112 VMA intein (including -1 and +1 extein residues)
GCFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGSETMYSVVQKSQHRAHKSDSSREMPELLKFTCNAT
HELVVRTPRSVRRLSRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPVSEGPERANELVESYRKASNKAYFEWT
IEARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMER
VTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRGNGVRNNLNTENPLWDAIIGLGFLKDGVKNIPSFLSTDNIGTRETF
LAGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVASVNAEPAKVDMNGTKHKISYAIYMSGGDVLLNVLSKC
AGSKKFRPAPVATFVRECQGFYFELQELKENDYYGITLSDDSDHQFLLANQVVVHNC |
| Block A |
CFAKGTNVLMADG 13 |
| Block B |
LLKFTCNATHELVV 83 |
| Block C |
LLGLWIGDG 219 |
| Block D |
VKNIPSFL 307 |
| Block E |
FLAGLIDSDG 327 |
| Block H |
TIHTSVRDGLVSLARSLGL 359 |
| Block F |
YGITLS**DDSDHQFL 445 |
| Block G |
NQVVVHNC 455 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
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| Comments |
Identical sequence to Spa VMA intein and Sce strains OUT7900(BAC66644), OUT7903 (BAC66645) |
| Date Submitted |
10/28/2005 |
| References |
Okuda 2003 |
