| Intein Name |
Mfl GyrA |
| Prototype Allele |
Mle-TN GyrA |
| Extein Name |
DNA gyrase subunit A |
| Intein Class |
Experimental |
| Organism Name |
Mycobacterium flavescens Fla0 |
| Organism Description |
taxon:1776, reference #930991 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
Y? |
| Insert Site Comments |
gyrA-a, After active site Tyr |
| Intein Size (aa) |
421 |
| Intein N-terminal |
GNDPPAAMRY/C |
| Intein C-terminal |
HN/TEARLTPLAM |
| Accession No. |
Q49166 in NCBI/protein, GYRA_MYCFV |
| Intein aa Sequence |
>gi|2501244|sp|Q49166|CAA92433.1| Mfl GyrA intein (including -1 and +1 extein residues)
YCVTGDALVRLPFGQSVRLRDVVAGARSSSDNAIDLKVLNRHGDPVVADKLFHSGEHETYTVRTAEGYEV
TGTANHPLLCLVDVGGVPTLLWKLTEEIRPGDHVVLQRTPPTEFGPADWQDAFEALHLGAFISEGFVSEN
RAGFNNLDREFFNAVLTAYDTIVGGPRYVSSRTIASDSLLHELDVHNLTALKKSRLGELVGQRSADKAVP
EWLWKAPAVVKRVFLQALFEGDGSCSALPRNTIQVSYSTRSGRLAKDIQQMLLEFGVISRRYVHATGEHK
VVLTSRAQAELFAAQIGFGGIKQAKLQGLLDALPQAAAGRDGDYVPGLAQFVRKHSGSRWVDKDWLNRHN
IDRLSRWQRDGAEILGRIADPDVRAIAQELTDGRFYYARVASVTDSGVQPVYSLRVDTDDHSFITNGFVS
HNT |
| Block A |
CVTGDALVRLPFG 13 |
| Block B |
GYEVTGTANHPLLC 79 |
| Block C |
HLGAFISEG 134 |
| Block D |
DKAVPEWL 212 |
| Block E |
FLQALFEGDG 232 |
| Block H |
TRSGRLAKDIQQMLLEFGV 266 |
| Block F |
PVYSLRV*DTDDHSFL 412 |
| Block G |
NGFVSHNT 422 |
| Initially Contributed by |
Stewart Cole |
| Contributor's Address |
Institut Pasteur
Unite de Genetique Moleculaire Bacterienne
28 rue du Dr. Rous
75724 Paris Cedex 15, France |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
|
| Independently Found By |
|
| Comments |
Partial gyrase extein sequence.
This intein splices in E.coli (E.Adam & F. Perler, unpublished data). |
| Date Submitted |
|
| References |
Fsihi 1996
Adam 2002 |
