| Intein Name |
Pho RtcB (Pho Hyp-2) |
| Prototype Allele |
Mja RtcB |
| Extein Name |
RNA terminal phosphate cyclase operon orfB |
| Intein Class |
Theoretical |
| Organism Name |
Pyrococcus horikoshii OT3 |
| Organism Description |
Thermophile, taxon:53953 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
N97 |
| Insert Site Comments |
rtcB-a |
| Intein Size (aa) |
390 |
| Intein N-terminal |
SPGGIGYDIN/C |
| Intein C-terminal |
HN/CGVRLIRTNL |
| Accession No. |
NT01PH1611 in Tigr, PH1602 |
| Intein aa Sequence |
>NT01PH1611 |PH1602| Tigr database| Pho RtcB (Pho Hyp-2) intein (including -1 and +1 extein residues)
NCLAPGTRVLTEHGYWLKIEEMPEKFKLQRLRVYNIEEGHNDFSKVVFVAEREVGSEEKA
IRIVTESGKVIEGSEDHPVLTPEGYVYLRNVKEGDYILVYPFEGVPYEEKKGVILDESAF
EGEDPQVVKFLRERNLIPLQWKDPKVGILARILGFALANGYISENDNLTFHGKEEVLREV
RKDLEELGIEAIVAEEDKLKVTSREFAFLLEKLGMAHDSIPEWIIEGPLWIKRNFLAGLF
GANGSIVEFKGDVPLPITLTHSRELLNDVSRILEGFKVRAKIKMGKNGSYQLVIEDEDSI
RNFLGRINYEYDPEKKARGLIAYAYLKFKELMKGNLMTFEEFARDRGYEGGFVAEKVIEV
KSVKPEYDKFYDIGVYHSAHNFIANGIVVHNC |
| Block A |
CLAPGTRVLTEHG 13 |
| Block B |
GKVIEGSEDHPVLT 80 |
| Block C |
ILGFALANG 159 |
| Block D |
HDSIPEWI 223 |
| Block E |
FLAGLFGANG 243 |
| Block H |
THSRELLNDVSRILEGFKV 277 |
| Block F |
KFYDIGV*YHSAHNFI 382 |
| Block G |
NGIVVHNC 391 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
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| Comments |
Motifs by Pietrokovski and Perler. Formally called Hyp-2 intein. In E.coli this gene is in an operon with rtcA - an RNA 3'-terminal phosphate cyclase - and rtcR the sigma-54 regulator of rtcA and rtcB (Pietrokovski, Genschik 98). |
| Date Submitted |
07/20/1998 |
| References |
Genschik 1997
Genschik 1998
Kawarabayasi 1998
Kawarabayasi 1998B
Kawarabayasi 2001 |
