| Intein Name |
Pgu-alt GLT1 |
| Prototype Allele |
Dhan GLT1 |
| Extein Name |
Glutamate synthase, alt. code 12 |
| Intein Class |
Theoretical |
| Organism Name |
Pichia (Candida) guilliermondii |
| Organism Description |
Fungi |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
K? |
| Insert Site Comments |
GLT1-a, R1183 of Dha GluSyn |
| Intein Size (aa) |
553 |
| Intein N-terminal |
IAMGCIMMRK/C |
| Intein C-terminal |
SN/CHLNTCPVGI |
| Accession No. |
http://www.broad.mit.edu/annotation/fungi/fgi |
| Intein aa Sequence |
>Broad Instidute genome sequencing project | Pgu-alt GLT1 intein (including -1 and +1 extein residues)
KCLASDTLVRTTQGLKKVKDVSVGEYLYGADNTPVLCIGANAPETGSLKEIVYEDFDSKKLVSFKCTPDHHLELVLANAA
PELSGSTVTWFSSCTGEHKKQDSTFDLESIVSSFYNDLVDSDDIITFDNVCEAIDVALDEHYHRGGSDRYSEKFNGFITQ
IANEELKSNPEFVRNALHEAAEKFMVEDAPKNFAVTSVSVETTSVESQSVCKSSCACGGVRKIVHQFETIEEAEMAYSLL
LSDVYDRVDPSFVSNGYKFQASVEALEQMCSKDILHNHLHMYRAPAEQVEPLTHSSSLPVDPYFLGLWLGEGSSDSATIS
TTDREVVVWLQQYVERLNKDKPAEASPLRLTKINGFKPGQQMANGFVQKVTVPTYTITSGESFTGGNWNPVYNSLKSMGL
LSNKSNGIPEAYKAADLQSRLALLAGLVDSDGCYVESYNSYRFMQKSELHKKIVYDLKEMATSCGIETTDIDVSESRLYL
GSSETTSAYVVYLGSGSEKLQQSLLLPRKKMVRIESCIQESTPFKVQDVANGEFRAIEVSGGKFQLDNGLQIANC |
| Block A |
CLASDTLVRTTQG 13 |
| Block B |
LVSFKCTPDHHLEL 73 |
| Block C |
FLGLWLGEG 311 |
| Block D |
NKDKPAEA 344 |
| Block E |
LLAGLVDSDG 431 |
| Block H |
ELHKKIVYDLKEMATSCGI 465 |
| Block F |
GEFRAI**EVSGGKFQ 544 |
| Block G |
NGLQIANC 554 |
| Initially Contributed by |
Tim Goodwin, Margi Butler, Russell Poulter |
| Contributor's Address |
Otago University
Dunedin, New Zealand |
| Contributor's Phone No. |
+64 3 4795688 |
| Contributor's FAX No. |
+64 3 4797866 |
| Contributor's Email address |
margi.butler@stonebow.otago.ac.nz |
| Independently Found By |
|
| Comments |
DNA translated using the “alternative yeast nuclear” code (no. 12) results in 3 changes of Leu to Ser at 319, 396, 502 (Bulter). |
| Date Submitted |
09/29/2005 |
| References |
Butler 2005B |
