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Pgu-alt GLT1 Intein
Intein Name Pgu-alt GLT1
Prototype Allele Dhan GLT1
Extein Name Glutamate synthase, alt. code 12
Intein Class Theoretical
Organism Name Pichia (Candida) guilliermondii
Organism Description Fungi
Domain of Life Eucarya
Endonuclease Activity
Endo Motif DOD
Location in Extein K?
Insert Site Comments GLT1-a, R1183 of Dha GluSyn
Intein Size (aa) 553
Intein N-terminal IAMGCIMMRK/C
Intein C-terminal SN/CHLNTCPVGI
Accession No. http://www.broad.mit.edu/annotation/fungi/fgi
Intein aa Sequence >Broad Instidute genome sequencing project | Pgu-alt GLT1 intein (including -1 and +1 extein residues)
KCLASDTLVRTTQGLKKVKDVSVGEYLYGADNTPVLCIGANAPETGSLKEIVYEDFDSKKLVSFKCTPDHHLELVLANAA
PELSGSTVTWFSSCTGEHKKQDSTFDLESIVSSFYNDLVDSDDIITFDNVCEAIDVALDEHYHRGGSDRYSEKFNGFITQ
IANEELKSNPEFVRNALHEAAEKFMVEDAPKNFAVTSVSVETTSVESQSVCKSSCACGGVRKIVHQFETIEEAEMAYSLL
LSDVYDRVDPSFVSNGYKFQASVEALEQMCSKDILHNHLHMYRAPAEQVEPLTHSSSLPVDPYFLGLWLGEGSSDSATIS
TTDREVVVWLQQYVERLNKDKPAEASPLRLTKINGFKPGQQMANGFVQKVTVPTYTITSGESFTGGNWNPVYNSLKSMGL
LSNKSNGIPEAYKAADLQSRLALLAGLVDSDGCYVESYNSYRFMQKSELHKKIVYDLKEMATSCGIETTDIDVSESRLYL
GSSETTSAYVVYLGSGSEKLQQSLLLPRKKMVRIESCIQESTPFKVQDVANGEFRAIEVSGGKFQLDNGLQIANC
Block A CLASDTLVRTTQG 13
Block B LVSFKCTPDHHLEL 73
Block C FLGLWLGEG 311
Block D NKDKPAEA 344
Block E LLAGLVDSDG 431
Block H ELHKKIVYDLKEMATSCGI 465
Block F GEFRAI**EVSGGKFQ 544
Block G NGLQIANC 554
Initially Contributed by Tim Goodwin, Margi Butler, Russell Poulter
Contributor's Address Otago University
Dunedin, New Zealand
Contributor's Phone No. +64 3 4795688
Contributor's FAX No. +64 3 4797866
Contributor's Email address margi.butler@stonebow.otago.ac.nz
Independently Found By
Comments DNA translated using the “alternative yeast nuclear” code (no. 12) results in 3 changes of Leu to Ser at 319, 396, 502 (Bulter).
Date Submitted 09/29/2005
References Butler 2005B

Last database update: 11/05/10

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