| Intein Name |
Bde-JEL423 RPC2 |
| Prototype Allele |
Bde-JEL197 RPB2 |
| Extein Name |
DNA-directed RNA polymerase III,second largest subunit |
| Intein Class |
Theoretical |
| Organism Name |
Batrachochytrium dendrobatidis JEL423 |
| Organism Description |
Chytrid fungus, isolate JEL423, taxon 403673 |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
I526 |
| Insert Site Comments |
RPB2-c, RPC2-b |
| Intein Size (aa) |
488 |
| Intein N-terminal |
SLQTSQWGMI/C |
| Intein C-terminal |
HN/CPSDTPEGEA |
| Accession No. |
AATT01000078 in NCBI/Nucleotide/wgs |
| Intein aa Sequence |
>gi|116294805|gb|AATT01000078.1|[116294805]| Bde-JEL423 RPC2 intein (including -1 and +1 extein residues)
ICLSGDTEVVLGNGMDIVLLTDLVDGACVSTIEPRTGVVSPSLIDHYFAK
DATRVLKITLDDGRVIKADPEHPLFAAKIDETTRGLTGQWLRVEELTVGH
HALLVSPQQAYCTSEQWTLTLSEKEVCTASTAQLAKQLSEIGLVDVPIPL
SKVKSVARLFGLVLSAGDFSGKLYVEKDEDVTAVNSDLTALGFSPAHWVR
YQDDQQKWFKCDDSNGKAICITLVPTANALLQTLGAHDAEKQHQYCLLPK
WLLDAPTSIKREFLGALFGGNGAHITISCNEGKWEPTMSALTQHTDADHL
ESTVVYLKQVATLLGMLNICSSVSSEKHITAEKTGYAVHLHVDNTAENLV
RFYEQVGYRYCFNKTSQSSAPVQWIKGSLFFIEQHRSKCQHAFELLHTGL
GTKTAVSAEINMPHHNPSHMLNVKEIPTAPADEYITWIEFKDRYVHKESP
RFVWVTIHSIEEAPAERLYDFNTVSQNHSFFANSIVSHNC |
| Block A |
CLSGDTEVVLGNG 13 |
| Block B |
GRVIKADPEHPLFA 75 |
| Block C |
LFGLVLSAG 166 |
| Block D |
YCLLPKWL 251 |
| Block E |
FLGALFGGNG 271 |
| Block H |
ESTVVYLKQVATLLGMLNI 318 |
| Block F |
RLYDFNTV*SQNHSFF 480 |
| Block G |
NSIVSHNC 489 |
| Initially Contributed by |
Russell Poulter, Margi Butler, Tim Goodwin |
| Contributor's Address |
|
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
timg@sanger.otago.ac.nz |
| Independently Found By |
|
| Comments |
The Bde-JEL423 RPC2 and Bde-JEL197 RPB2 local insertion site sequences are very similar, as are the intein sequences. However, the remainder of the exteins are not similar. Therefore, it looks like intein alleles have been recently transferred to non-allelic exteins. |
| Date Submitted |
10/27/2006 |
| References |
|
