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Mtu-T92 RecA Intein
Intein Name Mtu-T92 RecA
Prototype Allele Mtu-H37Rv RecA
Extein Name RecAA
Intein Class Theoretical
Organism Name Mycobacterium tuberculosis T92
Organism Description Taxon:515617
Domain of Life Eubacteria
Endonuclease Activity
Endo Motif DOD
Location in Extein Q251
Insert Site Comments recA-a
Intein Size (aa) 440
Intein N-terminal PHPGQGRQEQ/V
Intein C-terminal HN/CSPPFKQAEF
Accession No. ZP_03426083.1 in NCBI protein, /locus_tag="MtubT9_010100017928"
Intein aa Sequence >ZP_03426083.1 GI:215428164 | Mtu-T92 RecA intein (including -1 and +1 extein residues)
QVPRRGHSDLRSGHRYTHRIEDVVDGRKPIHVVAAAKDGTLHARPVVSWFDQGTRDVIGLRIAGGAIVWATPDHKVLTEY
GWRAAGELRKGDRVAQPRRFDGFGDSAPIPADHARLLGYLIGDGRDGWVGGKTPINFINVQRALIDDVTRIAATLGCAAH
PQGRISLAIAHRPGERNGVADLCQQAGIYGKLAWEKTIPNWFFEPDIAADIVGNLLFGLFESDGWVSREQTGALRVGYTT
TSEQLAHQIHWLLLRFGVGSTVRDYDPTQKRPSIVNGRRIQSKRQVFEVRISGMDNVTAFAESVPMWGPRGAALIQAIPE
ATQGRRRGSQATYLAAEMTDAVLNYLDERGVTAQEAAAMIGVASGDPRGGMKQVLGASRLRRDRVQALADALDDKFLHDM
LAEELRYSVIREVLPTRRARTFDLEVEELHTLVAEGVVVHNC
Block A VPRRGHSDLRSGH 13
Block B GAIVWATPDHKVLT 77
Block C LLGYLIGDG 123
Block D EKTIPNWF 201
Block E LLFGLFESDG 223
Block H TTSEQLAHQIHWLLLRFGV 257
Block F RTFDLEV**EELHTLV 432
Block G EGVVVHNC 441
Initially Contributed by AZHWAR RAGHUNATH & SUNTHARAJ
GOKULNATH
Contributor's Address Department of Biotechnology, Alagappa University, Karaikudi - 630 003 Tamilnadu
INDIA
Contributor's Phone No. +91-9944310533
Contributor's FAX No.
Contributor's Email address raghu_lifescience@hotmail.com
Independently Found By
Comments Block A Poorly Conserved (AR & SG). There is a 42 aa region centered at the standard RecA-a splice junction that lacks similarity to both RecA and the intein N-terminus suggesting an inactive intein or an incorrect sequence (FP). The remainder of the intein is 100% ID to the Mtu-H37Rv intein (FP).
Date Submitted 12/21/2009
References

Last database update: 11/05/10

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