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Dra-ATCC13939 Snf2 Intein
Intein Name Dra-ATCC13939 Snf2
Prototype Allele Dra Snf2
Extein Name SNF2/Rad54 helicase
Intein Class Experimental
Organism Name Deinococcus radiodurans R1, ATCC13939/Brooks & Murray strain
Organism Description Radiation and DNA damage resistent, taxon:1299
Domain of Life Eubacteria
Endonuclease Activity
Endo Motif DOD
Location in Extein K693
Insert Site Comments snf2-a, ATP/GTP-binding site motif A (P-loop)
Intein Size (aa) 343
Intein N-terminal ILADDMGLGK/A
Intein C-terminal HN/TLQT------
Accession No. AF512685 in GenBank
Intein aa Sequence >AF512685 |Brooks and Murray strain| Dra-ATCC13939 Snf2 intein (including -1 and +1 extein residues)
KAQPLDAKVLTPLGWRTMGELQVGDYVIGRNGQPTQIVGVYPQGERPIYRVTLTDGASVEA
DAEHLWNVNTPVRKKRGLPERTLTTAEIAADLQDAAGNLKHYLPLVEPVQFAGRDLPLDP
YTLGALLGDGCFVHGVEITSEDELVSALPLPAGVEARLGTRLTPTVSTSRLVTAGQWTPN
PLKDALRGLGLHGKSGRYKFIPPDYLLGSPAQRLAVLQGLLDTDGHAGVVVEYVSVSEHL
ARGVVELVQSLGGVARIRQKATSHTYGGEKKTGLAWRATLKLPPELEPFRLAAKRAAYRR
PTKYPPTRGIKSIEWVGHKPAQCIAVDAPDHLYVTEGYIVTHNT
Block A AQPLDAKVLTPLG 13
Block B GASVEADAEHLWNV 68
Block C YTLGALLGD 129
Block D YKFIPPDY 205
Block E VLQGLLDTDG 225
Block H SVSEHLARGVVELVQSLGG 254
Block F PAQCIAV*DAPDHLYV 334
Block G GYIVTHNT 344
Initially Contributed by Maurice W. Southworth
Contributor's Address New England Biolabs
Contributor's Phone No.
Contributor's FAX No. 1-978-921-1350
Contributor's Email address southworth@neb.com
Independently Found By
Comments Has 1 C after Gln 212 codon, while the TIGR sequenced strain has 2 cytosines, resulting in 2 ORFS (DR1258 & DR1259). Intein begins with Ala1.Position in extein based on comparison with DR1258.
Date Submitted 02/05/2002
References Makarova 2001
Southworth 2002
Brace 2010

Last database update: 11/05/10

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