| Intein Name |
Dra-ATCC13939 Snf2 |
| Prototype Allele |
Dra Snf2 |
| Extein Name |
SNF2/Rad54 helicase |
| Intein Class |
Experimental |
| Organism Name |
Deinococcus radiodurans R1, ATCC13939/Brooks & Murray strain |
| Organism Description |
Radiation and DNA damage resistent, taxon:1299 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
K693 |
| Insert Site Comments |
snf2-a, ATP/GTP-binding site motif A (P-loop) |
| Intein Size (aa) |
343 |
| Intein N-terminal |
ILADDMGLGK/A |
| Intein C-terminal |
HN/TLQT------ |
| Accession No. |
AF512685 in GenBank |
| Intein aa Sequence |
>AF512685 |Brooks and Murray strain| Dra-ATCC13939 Snf2 intein (including -1 and +1 extein residues)
KAQPLDAKVLTPLGWRTMGELQVGDYVIGRNGQPTQIVGVYPQGERPIYRVTLTDGASVEA
DAEHLWNVNTPVRKKRGLPERTLTTAEIAADLQDAAGNLKHYLPLVEPVQFAGRDLPLDP
YTLGALLGDGCFVHGVEITSEDELVSALPLPAGVEARLGTRLTPTVSTSRLVTAGQWTPN
PLKDALRGLGLHGKSGRYKFIPPDYLLGSPAQRLAVLQGLLDTDGHAGVVVEYVSVSEHL
ARGVVELVQSLGGVARIRQKATSHTYGGEKKTGLAWRATLKLPPELEPFRLAAKRAAYRR
PTKYPPTRGIKSIEWVGHKPAQCIAVDAPDHLYVTEGYIVTHNT |
| Block A |
AQPLDAKVLTPLG 13 |
| Block B |
GASVEADAEHLWNV 68 |
| Block C |
YTLGALLGD 129 |
| Block D |
YKFIPPDY 205 |
| Block E |
VLQGLLDTDG 225 |
| Block H |
SVSEHLARGVVELVQSLGG 254 |
| Block F |
PAQCIAV*DAPDHLYV 334 |
| Block G |
GYIVTHNT 344 |
| Initially Contributed by |
Maurice W. Southworth |
| Contributor's Address |
New England Biolabs |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
1-978-921-1350 |
| Contributor's Email address |
southworth@neb.com |
| Independently Found By |
|
| Comments |
Has 1 C after Gln 212 codon, while the TIGR sequenced strain has 2 cytosines, resulting in 2 ORFS (DR1258 & DR1259). Intein begins with Ala1.Position in extein based on comparison with DR1258. |
| Date Submitted |
02/05/2002 |
| References |
Makarova 2001
Southworth 2002
Brace 2010 |
