New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
Contact NEB About Us Site Map Request a Catalog OEM at NEB ISO International Orders Freezer Program Quick Order
Nph PolB-2 Intein
Intein Name Nph PolB-2
Prototype Allele Tli Pol-1
Extein Name DNA-directed DNA polymerase (alpha or family B)
Intein Class Theoretical
Organism Name Natronomonas pharaonis DSM 2160
Organism Description taxon:348780
Domain of Life Archaea
Endonuclease Activity
Endo Motif DOD
Location in Extein N1006
Insert Site Comments pol-b, Pol Motif B
Intein Size (aa) 409
Intein N-terminal QQSAVKVIMN/C
Intein C-terminal GQ/SLYGVLGWDR
Accession No. YP_326412 in NCBI/protein, NP1508A, GeneID:3703042
Intein aa Sequence >gi|76801404|ref|YP_326412.1|[76801404] Nph PolB-2 intein (including -1 and +1 extein residues)
NCFTPDTEVLTPDGVRNIRALDIGDEVYSLDPETMEMEVKPVVDTHAYPDYRGELVDIQTGEIDFRVTPNHRMLVRKNGT
NGITDDEYRFVEAGALDLATTYELPHDWAGPDGEDVTQIDLTELIDEEYEVWVRPEVHGRTFTAELGWKPRRVPKADIDT
VGYVFTADEFKQHREYIESVCEQSYIRRESGLKWIPRVYDGDDFLELLAWYVTEGNAHTSERKEFDGQRHGSSTVQIAQE
PVVADGGDTHATIGTLLDRMGFDYDNDNGSYQVTSKLLGDLFCTLGGGGSEDKRIPQLVFEASRRQKERFMQTLISGDGD
RQPNSWQYNTASERLRDDVLRLCAHLGVPASYSEESGVYRIYVTEDGTNTLRMDRSAERTTADDGVYCVTVEDNHTLLAG
RAGKFQFVGQS
Block A CFTPDTEVLTPDG 13
Block B EIDFRVTPNHRMLV 74
Block C LLAWYVTEG 214
Block D DKRIPQLV 298
Block E FMQTLISGDG 318
Block H
Block F GVYCVTV**EDNHTLL 397
Block G KFQFVGQS 410
Initially Contributed by Fran Perler
Contributor's Address
Contributor's Phone No.
Contributor's FAX No.
Contributor's Email address
Independently Found By
Comments
Date Submitted 10/27/2005
References Falb 2005

Last database update: 11/05/10

InBase Home Background Info Splicing mechanism Splicing motifs DOD Endo motifs
Intein registry Intein alleles Selected properties Blast against InBase  
Do you have an intein? Submitting data Bibliography Intein links NEB Home