| Intein Name |
Pfu KlbA |
| Prototype Allele |
Mja KlbA |
| Extein Name |
KlbA, kilB operon ORF A |
| Intein Class |
Theoretical |
| Organism Name |
Pyrococcus furiosus |
| Organism Description |
Thermophile, taxon:186497, DSM3638 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
G463 |
| Insert Site Comments |
kblA-a |
| Intein Size (aa) |
522 |
| Intein N-terminal |
FTAMNTGHDG/A |
| Intein C-terminal |
SN/CMGTIHANSA |
| Accession No. |
AAL81118 IN NCBI/protein, PF0994 |
| Intein aa Sequence |
>gi|18893047|gb|AAL81118.1|[18893047] Pfu KlbA intein (including -1 and +1 extein residues)
GALYDFSVIQLSNGRFVLIGDLVEELFKKYAEKIKTYKDLEYIELNEEDRFEVVSVSPDlKANKHVVS
RVWRRKVREGEKLIRIKTRTGNEIILTRNHPLFAFSNGDVVRKEAEKLKVGDRVAVMMRPPSPPQTKA
VVDPAIYVKISDYYLVPNGKGMIKVPNDGIPPEKAQYLLSVNSYPVKLVREVDEKLSYLAGVILGDGY
ISSNGYYISATFDDEAYMDAFVSVVSDFIPNYVPSIRKNGDYTIVTVGSKIFAEMLSRIFGIPRGRKS
MWDIPDVVLSNDDLMRYFIAGLFDADGYVDENGPSIVLVTKSETVARKIWYVLQRlGIISTVSRVKSR
GFKEGELFRVIISGVEDLAKFAKFIPLRHSRKRAKLMEILRTKKPYRGRRTYRVPISSDMIAPLRQML
GLTVAELSKLASYYAGEKVSESLIRHIEKGRVKEIRRSTLKGIALALQQIAKDVGNEEAWVRAKRLQl
IAEGDVYWDEVVSVEEVDPKELGIEYVYDLTVEDDHNYVANGILVSNC |
| Block A |
ALYDFSVIQLSNG 13 |
| Block B |
GNEIILTRNHPLAF 102 |
| Block C |
LAGVILGDG 202 |
| Block D |
MWDIPDVV 279 |
| Block E |
FIAGLFDADG 298 |
| Block H |
TKSETVARKIWYVLQRLGI 329 |
| Block F |
YVYDLTV**EDDHNYV 514 |
| Block G |
NGILVSNC 523 |
| Initially Contributed by |
Dennis Maeder |
| Contributor's Address |
COMB
701 E.Pratt St.
Baltimore MD 21202 |
| Contributor's Phone No. |
410-234-8871 |
| Contributor's FAX No. |
410-234-8896 |
| Contributor's Email address |
maeder@umbi.umd.edu |
| Independently Found By |
|
| Comments |
Very unusual splice site pair. CM/GTI… is suggested by extein alignment rather than SN/CMGTI… reported for Pho KlbA (Maeder). Putative secretory protein kinase (Maeder) or type II secretion system protein (TIGR). |
| Date Submitted |
08/28/1998 |
| References |
Maeder 1999 |
