New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
Contact NEB About Us Site Map Request a Catalog OEM at NEB ISO International Orders Freezer Program Quick Order
Pst VMA Intein
Intein Name Pst VMA
Prototype Allele Sce VMA
Extein Name Vacuolar ATPase (H+-transporting ATP synthase), subunit A
Intein Class Theoretical
Organism Name Pichia stipitis CBS 6054, taxon:322104
Organism Description Yeast
Domain of Life Eucarya
Endonuclease Activity
Endo Motif DOD
Location in Extein G282
Insert Site Comments VMA-a
Intein Size (aa) 449
Intein N-terminal SNSDVIIYVG/C
Intein C-terminal HN/CGERGNEMAE
Accession No. ABN64963 in NCBI/protein, PICST75615
Intein aa Sequence >gi|126094817|gb|ABN64963.1| Pst VMA intein (including -1 and +1 extein residues)
GCFAKGTKVLMANGDDKNIEDIAVGEEVLGKDGLPREVVALPRGRETMYEVSEKTQHRAETVFGTASYTCNATHK
LVLQTNQRVNITNHVLRGESQTSVTYFQMKTAVADGREIELPKLCTKSFQHSSHGRENAWKKAEVFASTISRDPI
DWTAEARDISRLGYHVRRATRQLWSPVLVEKEVLAPMIAKRGFDESIAPYMAYLVGLWVGDGYSDRATFSIDIQD
VEIHERIKDFASHAGLTPRIACYKKSRDATISLHNSETRGKNVRQNLNTGNLLWSLLAEICGKKENEMLFKLVPS
FLRSESIAVREYFISGLVDSDGHVKRDEADKCYSATVKTIYPAVRDGLVSVARSLGIQTSVSVEEAKEVNSVKHQ
ESYAIYMANSSALDSVLSKCAAPRKRAEEPVCVNREPHPYTFHMVEKEEDDFYGITLSEDSDHQFLLSNLALVHN
C
Block A CFAKGTKVLMANG 13
Block B TASYTCNATHKLVL 77
Block C LVGLWVGDG 211
Block D FKLVPSFL 301
Block E FISGLVDSDG 321
Block H TIYPAVRDGLVSVARSLGI 356
Block F YGITLS**EDSDHQFL 440
Block G NLALVHNC 450
Initially Contributed by Margie Butler
Contributor's Address Department of Biochemistry
University of Otago
PO Box 56
Dunedin
New Zealand/Aotearoa
Contributor's Phone No.
Contributor's FAX No.
Contributor's Email address margi.butler@stonebow.otago.ac.nz
Independently Found By S. Pietrokovski
Comments
Date Submitted 09/11/2006
References Bakhrat 2006
Jeffries 2007

Last database update: 11/05/10

InBase Home Background Info Splicing mechanism Splicing motifs DOD Endo motifs
Intein registry Intein alleles Selected properties Blast against InBase  
Do you have an intein? Submitting data Bibliography Intein links NEB Home