| Intein Name |
Afu-FRR0163 PRP8 |
| Prototype Allele |
Cne-AD PRP8 |
| Extein Name |
PRP8, pre-mRNA splicing factor |
| Intein Class |
Experimental |
| Organism Name |
Aspergillus fumigatus strain FRR0163 |
| Organism Description |
Human pathogenic fungus, taxon:5085 |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
A? |
| Insert Site Comments |
PRP8-a, same as A1578 in Sce PRP8 |
| Intein Size (aa) |
819 |
| Intein N-terminal |
SWEGLFWERA/C |
| Intein C-terminal |
HN/SGFEESMKFK |
| Accession No. |
AAV91021 in NCBI/protein, |
| Intein aa Sequence |
>gi|56418438|gb|AAV91021.1|[56418438] Afu-FRR0163 PRP8 intein (including -1 and +1 extein residues)
ACLAKGTRLLRYDGSEIEVQDVKEGDLLLGPDGGPRRAFNIVNGKDRLYRIKIGGSKEDLVVTPNHILVLHREKRARNVYTGPSVQGHIQRSENGHGNLPMLSSSPAAAHHPNNLVKNRGDFWSALKSAIAWVLHAERSSTGANMVRNVLNGTVGLTAHKESYTVTNPQQKGVYYTYVWGNPQRTSIKGHRDHPPVFLPTKEDAFSAAIAKSRELYSQSEVTLATLRQRFLAKSADGKGGEILVDANLPNIFLLWDKNRSNLKFRVLCSRNFKTYGRVYTFESMPSTNAEEPGYGDDDELPQVSAEERYDTVEMTAAEFASLSTEERSRYRVFRCPGFELPEQPVPVNPYFLGLWLGDDNHEKTTNHNIHEENVREFLVNHAAELDMYLAWQGLIDYATVANPAPMMVRLPPTNPDTIEHRPVVCQARQSIRKLRLAAKNIAQPEVVLSTSPRPESQMQPKRELPSNTETALRSEAEASSISAILDSKAGHSSLDTGDPNSDVVPESIPNDVADFGLDGVPELTSSGFSELTSDSELMRLIEQVERSSQGSTEEPSQASVVEQEADLNLLETDSEDEEADSADDDEFGDPEASEFRPEPESQLSQSHFSNRRRNHRLRTGRRVYGDLNGEEEGILLDQIVEQSEGSRVNSLLRALDALGIIAQKGTGPETNRKHIPSIYMKNSRSVRLAVLAGLIDSDGWYVYPENVLGFAQSERWHSKLFWDVVALARSLGLSVLTKRRMMWNPARTERYPQLFAQISGNVAEVPCLIARKKGVERLIPQTHSFMIKDISLEPEATEWAGFRVDKDQLYLRHDYLVLHNS |
| Block A |
CLAKGTRLLRYDG 13 |
| Block B |
KEDLVVTPNHILVL 69 |
| Block C |
YFLGLWLGD 357 |
| Block D |
RKHIPSIY 678 |
| Block E |
VLAGLIDSDG 698 |
| Block H |
RWHSKLFWDVVALARSLGL 732 |
| Block F |
ATEWAGFRVDKDQLYL 810 |
| Block G |
DYLVLHNS 820 |
| Initially Contributed by |
Jeremy Gray, Tim Goodwin, Margi Butler, Russell Poulter |
| Contributor's Address |
Department of Biochemistry
University of Otago
PO Box 56
Dunedin
New Zealand/Aotearoa |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
margi.butler@stonebow.otago.ac.nz |
| Independently Found By |
Paul Liu |
| Comments |
Identical inteins were submitted by Paul Liu and the Otago group, with the exception of the -10 N-extein residue which was Ser in the Otago sequence and Thr in the Liu sequence. |
| Date Submitted |
08/12/2004 |
| References |
Liu 2004
Butler 2005 |
