| Intein Name |
Mfl RecA |
| Prototype Allele |
Mle-TN RecA |
| Extein Name |
RecA |
| Intein Class |
Experimental |
| Organism Name |
Mycobacterium flavescens Fla0 |
| Organism Description |
strain=Fla0, taxon:1776, ref. #930991 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
G205 |
| Insert Site Comments |
recA-b |
| Intein Size (aa) |
364 |
| Intein N-terminal |
LREKIGVMFG/C |
| Intein C-terminal |
HN/SPETTTGGKA |
| Accession No. |
|
| Intein aa Sequence |
>Unpublished sequence obtained from Eric Adam, NEB. | strain Fla0 | Mfl RecA intein (including -1 and +1 extein residues)
GCFAYGTRGALADGTTEKIGKIVNQKMDVEVMSYDPDTDQVVPRKVVNWFNNGPAEQFLQFTVEKSGGNG
KSQFAATPNHLIRTPAGWTEAGDLVAGDRVMAAEPHRLSDQQFQVVLGSLMGDGNLSPNRRDRNGVRFRM
GHGAKQVDYLQWKTALLGNIKHSTHVNDKGATFVDFTPLPELAELQRAVYLGDGKKFLSEENFKALTPLA
LVFWYMDDGPFTVRSKGLQERTAGGSGRIEICVEAMSEGNRIRLRDYLRDTHGLDVRLRLSGAAGKSVLV
FSTASSAKFQELVAPYITPSMEYKLLPRFRGQGAVTPQFVEPTQRLVPARVLDVHVKPHTRSMNRFDIEV
EGNHNYFVDGVMVHNS |
| Block A |
CFAYGTRGALADG 13 |
| Block B |
KSQFAATPNHLIRT 83 |
| Block C |
VLGSLMGDG 123 |
| Block D |
-------- |
| Block E |
LQRAVYLGDG 193 |
| Block H |
FLSEENFKALTPLALVFWY 214 |
| Block F |
NRFDIEV**EGNHNYF 356 |
| Block G |
DGVMVHNS 365 |
| Initially Contributed by |
Eric Adam |
| Contributor's Address |
New England Biolabs, Inc. |
| Contributor's Phone No. |
1-978-927-5054 |
| Contributor's FAX No. |
1-978-921-1350 |
| Contributor's Email address |
perler@neb.com |
| Independently Found By |
|
| Comments |
Unpublished sequence by E.Adam from PCR product.
The Mfl RecA inteins from ATCC14474 and strain Fla0 are only 90% identical (aa). |
| Date Submitted |
07/25/1997 |
| References |
|
