| Intein Name |
Ssp DnaX |
| Prototype Allele |
Ssp DnaX |
| Extein Name |
DNA pol III Tau-subunit |
| Intein Class |
Experimental |
| Organism Name |
Synechocystis species, strain PCC6803 |
| Organism Description |
Cyanobacterium, taxon:1148 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
*DOD |
| Location in Extein |
E129 |
| Insert Site Comments |
helicase-b, putative helicase DEAD-box motif |
| Intein Size (aa) |
430 |
| Intein N-terminal |
CRYKVYVIDE/C |
| Intein C-terminal |
HN/CHMLSTAAFN |
| Accession No. |
S77213 in NCBI/protein, sll1360 |
| Intein aa Sequence |
>gi|7469304|pir||S77213| Ssp DnaX intein (including -1 and +1 extein residues)
ECLTGDSQVLTRNGLMSIDNPQIKGREVLSYNETLQQWEYKKVLRWLDRGEKQTLSIKTK
NSTVRCTANHLIRTEQGWTRAENITPGMKILSPASVDVDNLSQSTALTASLGGLSGAINY
EAINTDKKNTTLSLSLKKQKPQDPFVNADVAKNLIFQHFCSAKEEKLKVSNPIGEDIPTK
KATDFGISEQKKLHQGQNRWEQKFSVLSTEPCLGMEVLTIPTHIADSPACDGPTAPSSQN
GWNIKRQDWDVCHPKYDSQPIKAMGKVPSAVKPVVPQTLLMFSAQSNLEVKENKFLRNGS
RISLKKEWLGGTWTTVPSLFPNLGVHQFSYTQRAFSRKKINLLLNGLPIEDIPPVQNPIA
EALTAKPITTQKWEQWPPASGYRTWKSIPSPQWHTNFEEVESVTKGQVEKVYDLEVEDNH
NFVANGLLVHNC |
| Block A |
CLTGDSQVLTRNG 13 |
| Block B |
NSTVRCTANHLIRT 73 |
| Block C |
DOD FAMILY |
| Block D |
ENDONUCLEASE |
| Block E |
IS IN A |
| Block H |
DIFFERENT READING FRAME |
| Block F |
KVYDLEV**EDNHNFV 422 |
| Block G |
NGLLVHNC 431 |
| Initially Contributed by |
Paul Xiang-Qin Liu |
| Contributor's Address |
Biochemistry Department
Dalhousie University
Halifax, Nova Scotia B3H 4H7
Canada |
| Contributor's Phone No. |
902-494-1208 |
| Contributor's FAX No. |
902-494-1355 |
| Contributor's Email address |
pxqliu@dal.ca |
| Independently Found By |
Also submitted by Shmuel Pietrokovski (5/16/97). |
| Comments |
Dna X is related to RFC, but the inteins are not in the same position (Liu).
Called SPC pol III in Dalgaard 1997.
A DOD family homing endonuclease is present between Blocks B and F in a different reading frame than the intein splicing domain (Gorbalenya 1998) |
| Date Submitted |
07/09/1997 |
| References |
Kaneko 1995
Kaneko 1996
Kaneko 1996B
Kaneko 1997
Liu 1997
Dalgaard 1997
Pietrokovski 1998
Gorbalenya 1998 |
