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Ctr VMA Intein
Intein Name Ctr VMA
Prototype Allele Sce VMA
Extein Name Vacuolar ATPase (H+-transporting ATP synthase), subunit A
Intein Class Experimental
Organism Name Candida tropicalis (nucleus)
Organism Description Yeast
Domain of Life Eucarya
Endonuclease Activity
Endo Motif DOD
Location in Extein G283
Insert Site Comments VMA-a
Intein Size (aa) 471
Intein N-terminal SNSDVIIYVG/C
Intein C-terminal HN/CGERGNEMAE
Accession No. A46080 in NCBI/protein
Intein aa Sequence >gi|1076955|pir||A46080 Ctr VMA intein (including -1 and +1 extein residues)
GCFTKGTQVMMADGADKSIESIEVGDKVMGKDGMPREVVGLPRGYDDMYKVRQLSSTRRNAKSEGLMDFT
VSADHKLILKTKQDVKIATRKIGGNTYTGVTFYVLEKTKTGIELVKAKTKVFGHHIHGQNGAEEKAATFA
AGIDSKEYIDWIIEARDYVQVDEIVKTSTTQMINPVHFESGKLGNWLHEHKQNKSLAPQLGYLLGTWAGI
GNVKSSAFTMNSKDDVKLATRIMNYSSKLGMTCSSTESGELNVAENEEEFFNNLGAEKDEAGDFTFDEFT
DAMDELTINVHGAAASKKNNLLWNALKSLGFRAKSTDIVKSIPQHIAVDDIVVRESLIAGLVDAAGNVET
KSNGSIEAVVRTSFRHVARGLVKIAHSLGIESSINIKDTHIDAAGVRQEFACIVNLTGAPLAGVLSKCAL
ARNQTPVVKFTRDPVLFNFDLIKSAKENYYGITLAEETDHQFLLSNMALVHNC
Block A CFTKGTQVMMADG 13
Block B LMDFTVSADHKLIL 78
Block C LLGTWAGIG 210
Block D VKSIPQHI 325
Block E LIAGLVDAAG 345
Block H TSFRHVARGLVKIAHSLGI 379
Block F YGITLA**EETDHQFL 462
Block G NMALVHNC 472
Initially Contributed by Gary Dean
Contributor's Address University of Cincinnati
College of Medicine
Dept Mol. Genetics, Biochem., Micro
Cincinnati, OH 45267-0524
Contributor's Phone No.
Contributor's FAX No.
Contributor's Email address
Independently Found By Okuda 2003 suggests intein phylogenetically closer to Kla or Sun, not Sce.
Comments The Ctr VMA extein gene has a typical Candida tropicalis intron and is the first gene reported to have both an intron and an intein (Gu 1993).
Ctr VMA is a nuclear gene.
Date Submitted
References Gu 1993
Steuer 2004

Last database update: 11/05/10

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