| Intein Name |
Ctr VMA |
| Prototype Allele |
Sce VMA |
| Extein Name |
Vacuolar ATPase (H+-transporting ATP synthase), subunit A |
| Intein Class |
Experimental |
| Organism Name |
Candida tropicalis (nucleus) |
| Organism Description |
Yeast |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
G283 |
| Insert Site Comments |
VMA-a |
| Intein Size (aa) |
471 |
| Intein N-terminal |
SNSDVIIYVG/C |
| Intein C-terminal |
HN/CGERGNEMAE |
| Accession No. |
A46080 in NCBI/protein |
| Intein aa Sequence |
>gi|1076955|pir||A46080 Ctr VMA intein (including -1 and +1 extein residues)
GCFTKGTQVMMADGADKSIESIEVGDKVMGKDGMPREVVGLPRGYDDMYKVRQLSSTRRNAKSEGLMDFT
VSADHKLILKTKQDVKIATRKIGGNTYTGVTFYVLEKTKTGIELVKAKTKVFGHHIHGQNGAEEKAATFA
AGIDSKEYIDWIIEARDYVQVDEIVKTSTTQMINPVHFESGKLGNWLHEHKQNKSLAPQLGYLLGTWAGI
GNVKSSAFTMNSKDDVKLATRIMNYSSKLGMTCSSTESGELNVAENEEEFFNNLGAEKDEAGDFTFDEFT
DAMDELTINVHGAAASKKNNLLWNALKSLGFRAKSTDIVKSIPQHIAVDDIVVRESLIAGLVDAAGNVET
KSNGSIEAVVRTSFRHVARGLVKIAHSLGIESSINIKDTHIDAAGVRQEFACIVNLTGAPLAGVLSKCAL
ARNQTPVVKFTRDPVLFNFDLIKSAKENYYGITLAEETDHQFLLSNMALVHNC |
| Block A |
CFTKGTQVMMADG 13 |
| Block B |
LMDFTVSADHKLIL 78 |
| Block C |
LLGTWAGIG 210 |
| Block D |
VKSIPQHI 325 |
| Block E |
LIAGLVDAAG 345 |
| Block H |
TSFRHVARGLVKIAHSLGI 379 |
| Block F |
YGITLA**EETDHQFL 462 |
| Block G |
NMALVHNC 472 |
| Initially Contributed by |
Gary Dean |
| Contributor's Address |
University of Cincinnati
College of Medicine
Dept Mol. Genetics, Biochem., Micro
Cincinnati, OH 45267-0524 |
| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
Okuda 2003 suggests intein phylogenetically closer to Kla or Sun, not Sce. |
| Comments |
The Ctr VMA extein gene has a typical Candida tropicalis intron and is the first gene reported to have both an intron and an intein (Gu 1993).
Ctr VMA is a nuclear gene. |
| Date Submitted |
|
| References |
Gu 1993
Steuer 2004 |
