| Intein Name |
Cre RPB2 |
| Prototype Allele |
|
| Extein Name |
DNA-directed RNA polymerase II |
| Intein Class |
Theoretical |
| Organism Name |
Chlamydomonas reinhardtii (nucleus) |
| Organism Description |
Green algae, taxon:3055 |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
D1085 |
| Insert Site Comments |
RPB2-a, D1136 in Sce RPB2 CAA99357 |
| Intein Size (aa) |
431 |
| Intein N-terminal |
GLRFGEMERD/C |
| Intein C-terminal |
GN/CIISHGAAAF |
| Accession No. |
AAY89365 in NCBI/protein |
| Intein aa Sequence |
>gi|68300856|gb|DQ020659.1| AAY89365| Cre RPB2 intein (including -1 and +1 extein residues)
DCLSEEHQVLTNEGFLFLHEIEARKRDPALLFAGYDPAAEKIVYEPMDELIVKAAKTQTMVEFTQAAKSSRW
ADNDHSSGLSLVVTPGHDMYLRQGQAQNHWKSPFQKVKARDVLAAQETHDCVKFLSCAKNGVSVSDDLPK
PLADLDLDAAELTALLELYGYWLGDGSLTFQGNCGRDTVLFSPVKPQDHVWLEQRFRSLGFTLGVQYTRS
NENGRGEIKYDIIEPRIVALFHNEYRHKYDSGDQQLVELELEEAEAEDQERDFVGIATANSSVMSKEGLE
SAKWFAGWVWLLGKDYLRAILVGLSFADGAEAAQVGIIHTSSARFRDDTVRLALHAGYAATFDLMYKEGT
SRGIVRGKEIIATQDAWTVTYKDNPTYSEPMLSVKESVREIEYTGRTWCVSLPHTYIVTRRAHQMDGVVT
KASLPTILGNC |
| Block A |
CLSEEHQVLTNEG 13 |
| Block B |
GLSLVVTPGHDMYL 92 |
| Block C |
LYGYWLGDG 167 |
| Block D |
AKWFAGWV 290 |
| Block E |
ILVGLSFADG 310 |
| Block H |
TSSARFRDDTVRLALHAGY 339 |
| Block F |
RTWCVS****LPHTYI 408 |
| Block G |
LPTILGNC 432 |
| Initially Contributed by |
Tim Goodwin, Margi Butler, Russell Poulter |
| Contributor's Address |
Otago University
Dunedin, New Zealand |
| Contributor's Phone No. |
+64 3 4795688 |
| Contributor's FAX No. |
+64 3 4797866 |
| Contributor's Email address |
margi.butler@stonebow.otago.ac.nz |
| Independently Found By |
|
| Comments |
Nuclear gene intein (Butler).
Insertion site at equivalent position of D1136 in Sce RPB2 CAA99357 or D1096 of Tomato Q42877 |
| Date Submitted |
09/30/2005 |
| References |
Luo 2007 |
