| Intein Name |
Tag Pol-2 (Tsp-TY Pol-2) |
| Prototype Allele |
Tli Pol-1 |
| Extein Name |
DNA polymerase (alpha or family B) |
| Intein Class |
Experimental |
| Organism Name |
Thermococcus aggregans |
| Organism Description |
Thermophile, taxon:110163 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
N854 |
| Insert Site Comments |
pol-b, Pol Motif B |
| Intein Size (aa) |
538 |
| Intein N-terminal |
RQRAVKLLAN/S |
| Intein C-terminal |
HN/SYYGYMGYPK |
| Accession No. |
CAA73475.1 in NCBI/protein |
| Intein aa Sequence |
>gi|2293389|emb|CAA73475.1| Tag Pol-2 (Tsp-TY Pol-2) intein (including -1 and +1 extein residues)
NSILPNEWLPIIENGEVKFVKIGEFIDRYMEEQKDKVRTVDNTEVLEVDNIFAFSLNKESKKSEIKKVKA
LIRHKYKGEAYEVELNSGRKIHITRGHSLFTIRNGKIKEIWGEEVKVGDLIIVPKKVKLNEKEAVINIPE
LISKLPDEDTADVVMTTPVKGRKNFFKGMLRTLKWIFGEESKRIRTFNRYLFHLEELGFVKLLPRGYEVT
DWEGLKRYRQLYEKLVKNLRYNGNKREYLVRFNDIKDSVSCFPRKELEEWKIGTXKGFRXKCILKVDEDF
GKFLGYYVSEGYAGAQKNKTGGMSYSVKLYNENPNVLKDMKNIAEKFFGKVRVGKNCVDIPKKMAYLLAK
SLCGVTAENKRIPSIIFDSSEPVRWAFLRAYFVGDGDIHPSKRLRLSTKSELLANQLVFLLNSLGVSSIK
IGFDSGVYRVYINEDLPFLQTSRQKNTYYPNLIPKEVLEEIFGRKFQKNITFEKFKELADSGKLDKRKVK
LLDFLLNGDIVLDRVKNVEKREYEGYVYDLSVEDNENFLVGFGLLYAHNS |
| Block A |
SILPNEWLPIIEN 13 |
| Block B |
GRKIHITRGHSLFT 100 |
| Block C |
FLGYYVSEG 290 |
| Block D |
NKRIPSII 365 |
| Block E |
FLRAYFVGDG 385 |
| Block H |
TKSELLANQLVFLLNSLGV 415 |
| Block F |
YVYDLSV**EDNENFL 528 |
| Block G |
GLLYAHNS 539 |
| Initially Contributed by |
F. Perler for F. Niehaus |
| Contributor's Address |
Technical University Hamburg-Harburg
Biotechnology I, Technical Microbiology
Denickestr. 15, 21073 Hamburg, FRG |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
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| Contributor's Email address |
|
| Independently Found By |
|
| Comments |
This DNA Pol contains inteins in the 3 most conserved alpha Pol Regions (Perler).
Formerly called Thermococcus spp. TY and Tsp-TY Pol-2. |
| Date Submitted |
05/06/1997 |
| References |
Niehaus 1997 |
