| Intein Name |
Aae RIR2 |
| Prototype Allele |
|
| Extein Name |
Ribonucleoside- diphosphate reductase, beta subunit |
| Intein Class |
Theoretical |
| Organism Name |
Aquifex aeolicus strain VF5 |
| Organism Description |
Thermophilic chemolithoautotroph, taxon:63363 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
L229 |
| Insert Site Comments |
RIR2-a, Metal binding site |
| Intein Size (aa) |
346 |
| Intein N-terminal |
QIKYINRDEL/C |
| Intein C-terminal |
GN/CHVTLFRNII |
| Accession No. |
AAC07431 in NCBI/protein, aq_1505 |
| Intein aa Sequence |
>gi|7521621|pir||A70431| Aae RIR2 intein (including -1 and +1 extein residues)
LCFIEGTEVLTKRGFVDFRELREDDLVAQYDIETGEISWTKPYAYVERDYEGSMYRLKHPKSNWEVVATE
GHEFIVRNLKTGKERKEPIEKVKLHPYSAIPVAGRYTGEVEEYDLWELVSGKGITLKTRSAVKNKLTPIE
KLLIVLQADGTIDSKRNGKFTGFQQLKFFFSKYRKINEFEKILNECAPYGIKWKKYERQDGIAYTVYYPN
DLPIKPTKFFDEWVRLDEITEEWIREFVEELVKWDGHIPKDRNKKKVYYYSTKEKRNKDFVQALCALGGM
RTVVSRERNPKAKNPVYRIWIYLEDDYINTQTMVKEEFYYKGKVYCVSVPKGNIVVRYKDSVCIAGNC |
| Block A |
CFIEGTEVLTKRG 13 |
| Block B |
NWEVVATEGHEFIV 75 |
| Block C |
LLIVLQADG 149 |
| Block D |
TKFFDEWV 223 |
| Block E |
FVEELVKWDG 245 |
| Block H |
STKEKRNKDFVQALCALGG 278 |
| Block F |
KVYCVSV**PKGNIVV 335 |
| Block G |
SVCIAGNC 347 |
| Initially Contributed by |
Shmuel Pietrokovski |
| Contributor's Address |
Molecular Genetics Dept.
Weizmann Institute of Science
P.O. Box 26, Rehovot 76100, Israel
http://bioinfo.weizmann.ac.il/~pietro |
| Contributor's Phone No. |
972 (8) 934 2747 |
| Contributor's FAX No. |
972 (8) 934 4108 |
| Contributor's Email address |
Shmuel.Pietrokovski@weizmann.ac.il |
| Independently Found By |
|
| Comments |
Inteins are known in the alpha subunit of this enzyme from archaea, bacteria, a phage and a virus (Pietrokovski).
Has a Gly instead of His in Block G (Pietrokovski).
gene="nrdF" |
| Date Submitted |
03/27/1998 |
| References |
Deckert 1998 |
