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Tko TopA Intein
Intein Name Tko TopA
Prototype Allele Mja r-Gyr
Extein Name DNA topoisomerase I
Intein Class Theoretical
Organism Name Thermococcus kodakaraensis KOD1
Organism Description Thermophile, taxon:69014
Domain of Life Archaea
Endonuclease Activity
Endo Motif DOD
Location in Extein Y315
Insert Site Comments top-a, 2 aa before the catalytic Y residue
Intein Size (aa) 511
Intein N-terminal IAQKLYEKGY/C
Intein C-terminal HN/CSYPRTSSQK
Accession No. YP_183504 in NCBI/protein, TK1091
Intein aa Sequence >gi|57641026|ref|YP_183504.1|[57641026] Tko TopA intein (including -1 and +1 extein residues)
YCLHPDSLIPTPQGVKRIKELPEKGEVFALDFDLKLSRARYRLLERDADEPMYKVTLSDRTELYLTADHPVLVYRDDQLI
FVPAEELRENDQVVLFINRSEYSPRTESPTLLGFLLENATSMKDYILYDPEFGGVLRNRIKDAGLKTEILWRFRIREPTY
YKYLRGKMPVPIVRFLLEEGVVSIEELREVFRGFSYSTSLTPISFEFSEEFWYLFGLVAGDGHLAKKGAITIPAKDRTED
TVKAVKEIANSLQVPFAFDEKYKMIILRSKSLTRLFELLGCPYGNKTEIFRIPGEIMAKPEWMAAFLAGYYDADGHIGTK
PTGGKKSHSPQIVLTSKNRMAIYTVKQMWQLLGVGTYLWEKKDRNGNFMAYELKVYSRDAWRFYEVMKNHLRIKRKDLEH
VKEVAIRKRKAYSHHYSVLNVKSWEGKIKSSNVLWKKFDMSNQTAHGRGISLDKLQRIVDYLTDTDLRRIAMGDVYVLGI
RSIEKFHYRGKVYDLVVDQYHNFIANGVVVHNC
Block A CLHPDSLIPTPQG 13
Block B RTELYLTADHPVLV 72
Block C LFGLVAGDG 221
Block D
Block E FLAGYYDADG 314
Block H SKNRMAIYTVKQMWQLLGV 353
Block F KVYDLVV**DQYHNFI 503
Block G NGVVVHNC 512
Initially Contributed by Fran Perler
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Independently Found By
Comments Formly called Pyrococcus kodakaraensis.
Date Submitted 02/28/2005
References Fukui 2005

Last database update: 11/05/10

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