| Intein Name |
Tko TopA |
| Prototype Allele |
Mja r-Gyr |
| Extein Name |
DNA topoisomerase I |
| Intein Class |
Theoretical |
| Organism Name |
Thermococcus kodakaraensis KOD1 |
| Organism Description |
Thermophile, taxon:69014 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
Y315 |
| Insert Site Comments |
top-a, 2 aa before the catalytic Y residue |
| Intein Size (aa) |
511 |
| Intein N-terminal |
IAQKLYEKGY/C |
| Intein C-terminal |
HN/CSYPRTSSQK |
| Accession No. |
YP_183504 in NCBI/protein, TK1091 |
| Intein aa Sequence |
>gi|57641026|ref|YP_183504.1|[57641026] Tko TopA intein (including -1 and +1 extein residues)
YCLHPDSLIPTPQGVKRIKELPEKGEVFALDFDLKLSRARYRLLERDADEPMYKVTLSDRTELYLTADHPVLVYRDDQLI
FVPAEELRENDQVVLFINRSEYSPRTESPTLLGFLLENATSMKDYILYDPEFGGVLRNRIKDAGLKTEILWRFRIREPTY
YKYLRGKMPVPIVRFLLEEGVVSIEELREVFRGFSYSTSLTPISFEFSEEFWYLFGLVAGDGHLAKKGAITIPAKDRTED
TVKAVKEIANSLQVPFAFDEKYKMIILRSKSLTRLFELLGCPYGNKTEIFRIPGEIMAKPEWMAAFLAGYYDADGHIGTK
PTGGKKSHSPQIVLTSKNRMAIYTVKQMWQLLGVGTYLWEKKDRNGNFMAYELKVYSRDAWRFYEVMKNHLRIKRKDLEH
VKEVAIRKRKAYSHHYSVLNVKSWEGKIKSSNVLWKKFDMSNQTAHGRGISLDKLQRIVDYLTDTDLRRIAMGDVYVLGI
RSIEKFHYRGKVYDLVVDQYHNFIANGVVVHNC |
| Block A |
CLHPDSLIPTPQG 13 |
| Block B |
RTELYLTADHPVLV 72 |
| Block C |
LFGLVAGDG 221 |
| Block D |
|
| Block E |
FLAGYYDADG 314 |
| Block H |
SKNRMAIYTVKQMWQLLGV 353 |
| Block F |
KVYDLVV**DQYHNFI 503 |
| Block G |
NGVVVHNC 512 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
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| Comments |
Formly called Pyrococcus kodakaraensis. |
| Date Submitted |
02/28/2005 |
| References |
Fukui 2005 |
