New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
Contact NEB About Us Site Map Request a Catalog OEM at NEB ISO International Orders Freezer Program Quick Order
Oli DnaE-n Intein
Intein Name Oli DnaE-n
Prototype Allele Ssp DnaE-n
Extein Name DnaE pol subunit, DNA polymerase III alpha subunit
Intein Class Experimental
Organism Name Oscillatoria limnetica str. 'Solar Lake'
Organism Description Cyanobacterium, taxon:262926
Domain of Life Eubacteria
Endonuclease Activity
Endo Motif None
Location in Extein Y25?
Insert Site Comments dnaE-a, Beta and tau binding domains
Intein Size (aa) 112
Intein N-terminal FEQMVKFAEY/C
Intein C-terminal SN/CFNKSHSMAY
Accession No. AAP47640 in NCBI/protein,
Intein aa Sequence >gi|37784578|gb|AAP47640.1|[37784578] Oli DnaE-n N-terminal intein fragment (including -1 extein residue)
YCLSYNTEVLTVEYGPLPIGKIVDEQIHCRVYSVDENGFVYTQAIAQWHDRGYQEIFAYELADGSVIRATKDHQFMTEDG
QMFPIDEIWEKGLDLKKLPTVQDLPAAVGYTVS
Block A CLSYNTEVLTVEY N13
Block B GSVIRATKDHQFMT N76
Block C None
Block D None
Block E None
Block H None
Block F
Block G
Initially Contributed by Fran Perler
Contributor's Address
Contributor's Phone No.
Contributor's FAX No.
Contributor's Email address
Independently Found By
Comments This DnaE intein is a natuarlly split intein. Homolog of the catalytic alpha subunit of E. coli DNA polymerase III. BLOCKS A & B ARE IN Fragment N AND BLOCKS F & G ARE IN Fragment C.
Date Submitted 02/22/2005
References Caspi 2003
Dassa 2007

Last database update: 11/05/10

InBase Home Background Info Splicing mechanism Splicing motifs DOD Endo motifs
Intein registry Intein alleles Selected properties Blast against InBase  
Do you have an intein? Submitting data Bibliography Intein links NEB Home