New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
Contact NEB About Us Site Map Request a Catalog OEM at NEB ISO International Orders Freezer Program Quick Order
Spu PRP8 Intein
Intein Name Spu PRP8
Prototype Allele
Extein Name PRP8, pre-mRNA splicing factor
Intein Class Theoretical
Organism Name Spizellomyces punctatus
Organism Description Chytrid fungus,
Domain of Life Eucarya
Endonuclease Activity
Endo Motif DOD
Location in Extein H?
Insert Site Comments PRP8-c, same as H1947 in Sce PRP8
Intein Size (aa) 473
Intein N-terminal KGMLDPLEVH/C
Intein C-terminal HN/CLDFPNIVIK
Accession No.
Intein aa Sequence >Umpublished sequence from Tim Goodwin, Russell Poulter, Margi Butler | Spu PRP8 intein (including -1 and +1 extein residues)
HCLAAGTKVMVVRNGCLLNVKVDELVQGDVLLDERGGATRINTPVVSGNRPLLRVSGRTHGLVSQEYTVTEGHLLSLRCE
SEPYVFTTGYPSGTVRFQYAAYKNDGSVEWQSAEFKHNKDGQLVRISEMDQEVGLPTSEQKDIKIQILDWWKQQADRVEL
RATVNITALEYMHLDADHPLKTKFGGYRGRVNDGWPVELSRSSSGVTTVLDPYFLGIWLGNGSTSANSVRVSISKDDVDI
VDKLRSIFLPQGFTIMVAGASTTAYDVDICDPISQSNRLKDALFEAGLIGEGSAKHIPVGYMHAGKDVRQRLLAGLIDAN
GVQSTRTDNGSLEHVMQGMEHMPSEHIERIIKNHQPLNVWIFGQRQMEERLFEDVVQLARSLEMMQTVSPDKLPKSNNTD
SHKAIILSGYDQEEVSSHVALKHKQLGPYCLLKGLDQNRIALTVERAEAGTYFRFSVDGYQSRFLLADWTVVHNC
Block A CLAAGTKVMVVRN 13
Block B SQEYTVTEGHLLSL 76
Block C FLGIWLGNG 221
Block D AKHIPVGY 300
Block E LLAGLIDANG 320
Block H
Block F TYFRFSV**DGYQSRF 463
Block G DWTVVHNC 474
Initially Contributed by Tim Goodwin, Russell Poulter, Margi Butler
Contributor's Address
Contributor's Phone No. +64 3 4795688
Contributor's FAX No. +64 3 4797866
Contributor's Email address margi.butler@stonebow.otago.ac.nz
Independently Found By
Comments First seen as a partial intein in three EST entries
(EB746545, EB743686, EB746610) but no adjacent extein sequences were available. Tim James, of Duke University kindly provided us (BUTLER) with
Spu DNA for inverse PCR and sequencing. This is the third intein insertion site in PRP8.
Date Submitted 11/06/2006
References

Last database update: 11/05/10

InBase Home Background Info Splicing mechanism Splicing motifs DOD Endo motifs
Intein registry Intein alleles Selected properties Blast against InBase  
Do you have an intein? Submitting data Bibliography Intein links NEB Home