| Intein Name |
Spu PRP8 |
| Prototype Allele |
|
| Extein Name |
PRP8, pre-mRNA splicing factor |
| Intein Class |
Theoretical |
| Organism Name |
Spizellomyces punctatus |
| Organism Description |
Chytrid fungus, |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
H? |
| Insert Site Comments |
PRP8-c, same as H1947 in Sce PRP8 |
| Intein Size (aa) |
473 |
| Intein N-terminal |
KGMLDPLEVH/C |
| Intein C-terminal |
HN/CLDFPNIVIK |
| Accession No. |
|
| Intein aa Sequence |
>Umpublished sequence from Tim Goodwin, Russell Poulter, Margi Butler | Spu PRP8 intein (including -1 and +1 extein residues)
HCLAAGTKVMVVRNGCLLNVKVDELVQGDVLLDERGGATRINTPVVSGNRPLLRVSGRTHGLVSQEYTVTEGHLLSLRCE
SEPYVFTTGYPSGTVRFQYAAYKNDGSVEWQSAEFKHNKDGQLVRISEMDQEVGLPTSEQKDIKIQILDWWKQQADRVEL
RATVNITALEYMHLDADHPLKTKFGGYRGRVNDGWPVELSRSSSGVTTVLDPYFLGIWLGNGSTSANSVRVSISKDDVDI
VDKLRSIFLPQGFTIMVAGASTTAYDVDICDPISQSNRLKDALFEAGLIGEGSAKHIPVGYMHAGKDVRQRLLAGLIDAN
GVQSTRTDNGSLEHVMQGMEHMPSEHIERIIKNHQPLNVWIFGQRQMEERLFEDVVQLARSLEMMQTVSPDKLPKSNNTD
SHKAIILSGYDQEEVSSHVALKHKQLGPYCLLKGLDQNRIALTVERAEAGTYFRFSVDGYQSRFLLADWTVVHNC |
| Block A |
CLAAGTKVMVVRN 13 |
| Block B |
SQEYTVTEGHLLSL 76 |
| Block C |
FLGIWLGNG 221 |
| Block D |
AKHIPVGY 300 |
| Block E |
LLAGLIDANG 320 |
| Block H |
|
| Block F |
TYFRFSV**DGYQSRF 463 |
| Block G |
DWTVVHNC 474 |
| Initially Contributed by |
Tim Goodwin, Russell Poulter, Margi Butler |
| Contributor's Address |
|
| Contributor's Phone No. |
+64 3 4795688 |
| Contributor's FAX No. |
+64 3 4797866 |
| Contributor's Email address |
margi.butler@stonebow.otago.ac.nz |
| Independently Found By |
|
| Comments |
First seen as a partial intein in three EST entries
(EB746545, EB743686, EB746610) but no adjacent extein sequences were available. Tim James, of Duke University kindly provided us (BUTLER) with
Spu DNA for inverse PCR and sequencing. This is the third intein insertion site in PRP8. |
| Date Submitted |
11/06/2006 |
| References |
|
|
