| Intein Name |
Pho IF2 |
| Prototype Allele |
Mja IF2 |
| Extein Name |
Translation initiation factor |
| Intein Class |
Theoretical |
| Organism Name |
Pyrococcus horikoshii OT3 |
| Organism Description |
Thermophile, taxon:53953 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
K22 |
| Insert Site Comments |
IF2-a, ATP/GTP-binding site motif A (P-loop) |
| Intein Size (aa) |
444 |
| Intein N-terminal |
AVLGHVDHGK/C |
| Intein C-terminal |
HN/TTLLDKIRKT |
| Accession No. |
O58822 in NCBI/protein, PH1095 |
| Intein aa Sequence |
>gi|6016319|sp|O58822| Pho IF2 intein (including -1 and +1 extein residues)
KCLLPEERVILPDYGPITLEELFNMTKETVFKDEEKEVRKLGIRMPVAGVDGRVRLLEGPYVWKVRYKGK
MLRVKLKDWHSVAVTPEHPFLTTRGWVRADQLKPGDYVAVPKILPGKDDKEKFLQYVHEKLKGKVHIKLP
SSDEEWETFFYFAGTIFGRENSVNPEGLTHEVKALLELFKVLFEYPREVLRVLFMAPVRYVANFLRGFFD
INGYVNGEELRVEVRGAPHEVLEELSLILLRLGIVSKIYPTSLAISGRRNLELFRRYIGFSEKQKAKELE
GIIRRSENSESYPIFEELRRIRLLFGFTRAELSSTIPLYSKYESKEAPSYEILMKILNTIEKGSKDLNKK
ITILEGRVRDHEYIEEFKREGLIKDGKLTELGKELLEVWRNREFDSRDVNYLRNIIENFVFLPVEKIEEF
EYDGYVYDVTTETHNFIANGILVHNT |
| Block A |
CLLPEERVILPDY 13 |
| Block B |
WHSVAVTPEHPFLT 91 |
| Block C |
FAGTIFGRE 159 |
| Block D |
|
| Block E |
FLRGFFDING 212 |
| Block H |
GAPHEVLEELSLILLRLGI 243 |
| Block F |
YVYDVTT***ETHNFI 436 |
| Block G |
NGILVHNT 445 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
|
| Comments |
Intein insertion site numbered using Met1 as listed in above Acc. No.
Motifs by Pietrokovski and Perler. |
| Date Submitted |
07/20/1998 |
| References |
Kawarabayasi 1998
Kawarabayasi 1998B
Kawarabayasi 2001 |