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Mka EF2 Intein
Intein Name Mka EF2
Prototype Allele Mja IF2
Extein Name Translation elongation/release factor (GTPase)
Intein Class Theoretical
Organism Name Methanopyrus kandleri AV19
Organism Description Thermophile, taxon:190192
Domain of Life Archaea
Endonuclease Activity
Endo Motif DOD
Location in Extein K35
Insert Site Comments IF2-a, ATP/GTP-binding site motif A (P-loop)
Intein Size (aa) 522
Intein N-terminal GIIAHIDHGK/C
Intein C-terminal HN/TTLSDQLLAG
Accession No. AAM01894 in NCBI/protein, fusA
Intein aa Sequence >gi|19887115|gb|AAM01894.1|[19887115] Mka EF2 intein (including -1 and +1 extein residues)
KCVAPETKICLADGRFVRADELFEELKERGRLVKCDESEEVYELREPVGVSSLDKDAVEIVEGKITHVWRLKADKLVEVE
VKNGRSIRTTPEHKFLVLDPSGEIVEKRADELEIGDYIVCTQKLVHEGMSEEELKREVFRRLGRDFFVHLPEEEAESVLE
LAKERGIKALWETLEVDIEENSFYYQLRKGRIRADILVDLAEELGLDLADLYDAVEVSYRSNTKSTKPIRLPEPEDLFYL
AGLMFGDGCWNQLTNGSEAIQGEVKRIASDMGLEVRVRRYEGKTARIDFPETVPRILEALFDYPRRKKAHRIRVNDFLTR
APLDCIAEFIRGYFDADGTVEEGRSAVSVTSVSREFLEDLQLLLQKFDVASYLREGDGAYTLYVSGARSLERFPGFREPE
KAEKLKKLMEKASSSELEKVPISGEILREVRGDVPTTRMFNCYSNYEGGQVGLTKSSLEKVISTLEAVGVEGEALERLKA
LARDDVCFLEVVRVEEVEYDGYVYDFTVEEHHNFAAEGFVVHNT
Block A CVAPETKICLADG 13
Block B GRSIRTTPEHKFLV 96
Block C
Block D
Block E
Block H
Block F YVYDFTV**EEHHNFA 514
Block G EGFVVHNT 523
Initially Contributed by Fran Perler
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Independently Found By
Comments
Date Submitted 02/28/2005
References Slesarev 2002

Last database update: 11/05/10

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