| Intein Name |
Tli Pol-1 |
| Prototype Allele |
Tli Pol-1 |
| Extein Name |
DNA polymerase (alpha or family B) |
| Intein Class |
Experimental |
| Organism Name |
Thermococcus litoralis |
| Organism Description |
Thermophile, taxon:2265 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
PI-TliII |
| Endo Motif |
DOD |
| Location in Extein |
N494 |
| Insert Site Comments |
pol-b, Pol Motif B |
| Intein Size (aa) |
538 |
| Intein N-terminal |
RQRAIKLLAN/S |
| Intein C-terminal |
HN/SYYGYMGYPK |
| Accession No. |
S42459 in NCBI/protein |
| Intein aa Sequence |
>gi|543522|pir||S42459 Tli Pol-1 intein (including -1 and +1 extein residues)
NSILPNEWLPIIENGEIKFVKIGEFINSYMEKQKENVKTVENTEVLEVNNLFAFSFNKKIKESEVKKVKA
LIRHKYKGKAYEIQLSSGRKINITAGHSLFTVRNGEIKEVSGDGIKEGDLIVAPKKIKLNEKGVSINIPE
LISDLSEEETADIVMTISAKGRKNFFKGMLRTLRWMFGEENRRIRTFNRYLFHLEKLGLIKLLPRGYEVT
DWERLKKYKQLYEKLAGSVKYNGNKREYLVMFNEIKDFISYFPQKELEEWKIGTLNGFRTNCILKVDEDF
GKLLGYYVSEGYAGAQKNKTGGISYSVKLYNEDPNVLESMKNVAEKFFGKVRVDRNCVSISKKMAYLVMK
CLCGALAENKRIPSVILTSPEPVRWSFLEAYFTGDGDIHPSKRFRLSTKSELLANQLVFLLNSLGISSVK
IGFDSGVYRVYINEDLQFPQTSREKNTYYSNLIPKEILRDVFGKEFQKNMTFKKFKELVDSGKLNREKAK
LLEFFINGDIVLDRVKSVKEKDYEGYVYDLSVEDNENFLVGFGLLYAHNS |
| Block A |
SILPNEWLPIIEN 13 |
| Block B |
GRKINITAGHSLFT 100 |
| Block C |
LLGYYVSEG 290 |
| Block D |
NKRIPSVI 365 |
| Block E |
FLEAYFTGDG 385 |
| Block H |
TKSELLANQLVFLLNSLGI 415 |
| Block F |
YVYDLSV**EDNENFL 528 |
| Block G |
GLLYAHNS 539 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
New England Biolabs |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
perler@neb.com |
| Independently Found By |
|
| Comments |
Using the convention of naming endos in order of discovery, Tli Pol-1 encodes PI-TliII (Perler).
This intein splices in E.coli (M.Southworth & F. Perler, unpublished data). |
| Date Submitted |
|
| References |
Perler 1992
Hodges 1992 |
|
