| Intein Name |
Cne-A PRP8 (Fne-A PRP8) |
| Prototype Allele |
Cne-AD PRP8 |
| Extein Name |
PRP8, pre-mRNA splicing factor |
| Intein Class |
Experimental |
| Organism Name |
Filobasidiella neoformans (Cryptococcus neoformans) Serotype A, PHLS_8104 |
| Organism Description |
Yeast, human pathogen |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
None |
| Location in Extein |
A? |
| Insert Site Comments |
PRP8-a, same as A1578 in Sce PRP8 |
| Intein Size (aa) |
171 |
| Intein N-terminal |
TWEGLFWEKA/C |
| Intein C-terminal |
HN/SGFEE----- |
| Accession No. |
AAR00330in NCBI/protein, |
| Intein aa Sequence |
>gi|37695591|gb|AAR00330.1|[37695591] Cne-A PRP8 (Fne-A PRP8) intein (including -1 and +1 extein residues)
ACLQNGTRLLRADGSEVLVEDVQEGDQLLGPDGTSRTASKIVRGEERLYRIKTHEGLEDLVCTHNHILSM
YKERFGREGAHSPSAGTSLTESHERVDVTVDDFVRLPQQEQQKYKLFRSTDFVRREQPSASKLATLLHIN
SIELEEEPTKWSGFVVDKDSLYLRYDYLVLHNS |
| Block A |
CLQNGTRLLRADG 13 |
| Block B |
LEDLVCTHNHILSM 69 |
| Block C |
None |
| Block D |
None |
| Block E |
None |
| Block H |
None |
| Block F |
PTKWSGFVVDKDSLYL 162 |
| Block G |
DYLVLHNS 172 |
| Initially Contributed by |
Margi Butler |
| Contributor's Address |
Department of Biochemistry
University of Otago
PO Box 56
Dunedin
New Zealand/Aotearoa |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
margi.butler@stonebow.otago.ac.nz |
| Independently Found By |
|
| Comments |
The organism is also called Cryptococcus neoformans var grubii.
The Fne PRP8 gene has not yet been fully described.
This gene also contains an intron.
Same aa seq for Cne strains Cn 3511 & JEC 21, with 1 silent nt change (Butler 2001).Same intein sequence in strain H99 and IUM93-3231 |
| Date Submitted |
04/27/2003 |
| References |
Butler 2001
Butler 2005
Loftus 2005 |
