| Intein Name |
MP-Mcjw1 DnaB |
| Prototype Allele |
|
| Extein Name |
DnaB helicase |
| Intein Class |
Theoretical |
| Organism Name |
Mycobacteriophage CJW1 |
| Organism Description |
Bacteriophage,taxon:205869 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
K92 |
| Insert Site Comments |
dnaB-f, ATP/GTP-binding site motif A (P-loop) |
| Intein Size (aa) |
331 |
| Intein N-terminal |
WIELARKNGK/A |
| Intein C-terminal |
HN/TELLAGIMLY |
| Accession No. |
AAN01623.1 in NCBI/protein, gp8 |
| Intein aa Sequence |
>gi|29424612|gb|AAN01623.1|[29424612] MP-Mcjw1 DnaB intein (including -1 and +1 extein residues)
KALDVETPILTGNGWKKMGDIQVGDYVHAADGTLARVSYVSERHWRDCFSVQFADGAELV
ASDHHLWAVNDRLKGERVIDTAELYRTQTYGARGDRRYTVTVPEALDRDEAPLPLDPYIL
GAWLGDGTATRAEFTSEDPEIFAAIEAAGYPLSYDYASGNARTRGAKGLVAVLRKMGVLG
AKHVPQDYLIGSRAQRLALLQGLMDTDGSVITGPNTPRVEFCNTNRDLAEAALFLARSLG
WKATLKESRARLNGKDCGPRFRVSWTAYSDMSPFRLQRKSEKLAAAPARATRARTNTITS
VTPVPTVETVCIQIDHPSHVFLAGKSLTPTHNT |
| Block A |
ALDVETPILTGNG 13 |
| Block B |
GAELVASDHHLWAV 68 |
| Block C |
ILGAWLGDG 126 |
| Block D |
AKHVPQDY 197 |
| Block E |
LLQGLMDTDG 207 |
| Block H |
NTNRDLAEAALFLARSLGW 240 |
| Block F |
ETVCIQI*DHPSHVFL 320 |
| Block G |
SLTPTHNT 332 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
New England Biolabs, Inc. |
| Contributor's Phone No. |
1-978-927-5054 |
| Contributor's FAX No. |
1-978-921-1350 |
| Contributor's Email address |
perler@neb.com |
| Independently Found By |
|
| Comments |
Has an Ala instead of the conserved intein N-terminal Ser/Cys.
Note that the Momega and Mcjw1 DnaB inteins are very similar, but inserted into different K/T sites in the phage DnaB extein. |
| Date Submitted |
04/28/2003 |
| References |
Pedulla 2003 |
