| Intein Name |
Pab RtcB (Pab Hyp-2) |
| Prototype Allele |
Mja RtcB |
| Extein Name |
RNA terminal phosphate cyclase operon orfB |
| Intein Class |
Theoretical |
| Organism Name |
Pyrococcus abyssi |
| Organism Description |
Thermophile, strain Orsay, taxon:29292 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
N97 |
| Insert Site Comments |
rtcB-a |
| Intein Size (aa) |
436 |
| Intein N-terminal |
SPGGIGYDIN/C |
| Intein C-terminal |
HN/CGVRLIRTNL |
| Accession No. |
C75175 in NCBI/protein, PAB0383 |
| Intein aa Sequence |
>gi|7517838|pir||C75175| Pab RtcB (Pab Hyp-2) intein (including -1 and +1 extein residues)
NCLAPGSKVLTEHGYWLKVEELPEKFKLQGVKVYNLDEGHNDTSNVAFVAEREVETGEMAVRVTTESGRI
IEGSEDHPVLTPEGYVYLGNLKEGNLVIVYPFEGVEYEERKGVILDEDAFKDEDPQVLSFLREKGLVPLR
WDDPRIGTIARILGFAFGDGYLGEMGGRLTLTFYGKEETLRELKKDLERLGISANLYVRESIETTSGHSE
GKSLSIELRVTSRSFALFLEKLGMPRGKKTEKAYRVPGWILEAPLWVKRNFLAGLFAADGSIVEFKGNTP
LPINLTQSKSDELAENLVEFLGDVAKLLAEFGIETTLYEVKSKKGVTYRLSIVGEDSIRTFVERINYEYD
PEKKVKGLIAAAYLKLKERIVKEAHEAVKDDFPTFEEFAKERGYEGGFVAEKVVKVERVKPEYTKFYDIG
VYHEAHNFIANGIVVHNC |
| Block A |
CLAPGSKVLTEHG 13 |
| Block B |
GRIIEGSEDHPVLT 80 |
| Block C |
ILGFAFGDG 159 |
| Block D |
AYRVPGWI 249 |
| Block E |
FLAGLFAADG 269 |
| Block H |
ENLVEFLGDVAKLLAEFGI 312 |
| Block F |
KFYDIGV*YHEAHNFI 428 |
| Block G |
NGIVVHNC 437 |
| Initially Contributed by |
Joël Quérellou |
| Contributor's Address |
Ifremer, BP 70
29280 Plouzane
France |
| Contributor's Phone No. |
33 2 98 22 40 01 |
| Contributor's FAX No. |
33 2 99 22 46 00 |
| Contributor's Email address |
Jquerell@ifremer.fr |
| Independently Found By |
|
| Comments |
Formally called Hyp-2 intein. In E.coli this gene is in an operon with rtcA - an RNA 3'-terminal phosphate cyclase - and rtcR the sigma-54 regulator of rtcA and rtcB (Pietrokovski, Genschik 98). |
| Date Submitted |
06/26/1999 |
| References |
Genschik 1997
Genschik 1998
Lecompte 2001
Cohen 2003 |
