New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
Contact NEB About Us Site Map Request a Catalog OEM at NEB ISO International Orders Freezer Program Quick Order
Nsp-JS614 TOPRIM Intein
Intein Name Nsp-JS614 TOPRIM
Prototype Allele
Extein Name Topoisomerase/primase domain
Intein Class Theoretical
Organism Name Nocardioides species JS614
Organism Description taxon:196162
Domain of Life Eubacteria
Endonuclease Activity
Endo Motif DOD
Location in Extein H61
Insert Site Comments TOPRIM-a
Intein Size (aa) 312
Intein N-terminal NVNPSRGFFH/C
Intein C-terminal GN/CFGCSEGGNV
Accession No. ABL81457 in NCBI/protein, Noca_1947, NocaDRAFT_1542
Intein aa Sequence >gi|71156494|gb|EAO06904.1|ABL81457 |[71156494] Nsp-JS614 TOPRIM intein (including -1 and +1 extein residues)
HCLAGETKVLTWDGPRPISELAGGTHRILNVHGDWAEAPLKSYGVQRLWRIVVTRNRERKELFATDGHRWFVPSGRTGSK
HREVLTKELKAGDRLVSKFPRSRIQRTTPSPFGIAHGFTYGDGTRSGTGSMALLRPPKDLAMLKWFSNSHTSASGDNLLV
HHLPRFFKELPDPDESVSYLYGWLAGYFAADGCVAADGTVILNSARRENLEFVRMVTTRLGIATYGITTQSRVGLGKEHS
DLHRVHFVNDDLTESFFLLDEHRRRFAEATKKYARRGWVVQAVEETDRVEEVYCAEVEDGHAFTLEDNILTGNC
Block A CLAGETKVLTWDG 13
Block B RKELFATDGHRWFV 71
Block C AHGFTYGDG 122
Block D FKELPDPD 173
Block E WLAGYFAADG 191
Block H SARRENLEFVRMVTTRLGI 221
Block F EVYCAEV**EDGHAFT 303
Block G DNILTGNC 313
Initially Contributed by Fran Perler
Contributor's Address
Contributor's Phone No.
Contributor's FAX No.
Contributor's Email address
Independently Found By
Comments topoisomerase/primase domain, a nucleotidyl transferase/hydrolase domain.
Date Submitted 05/05/2006
References

Last database update: 11/05/10

InBase Home Background Info Splicing mechanism Splicing motifs DOD Endo motifs
Intein registry Intein alleles Selected properties Blast against InBase  
Do you have an intein? Submitting data Bibliography Intein links NEB Home