New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
Contact NEB About Us Site Map Request a Catalog OEM at NEB ISO International Orders Freezer Program Quick Order
Pfu IF2 Intein
Intein Name Pfu IF2
Prototype Allele Mja IF2
Extein Name Translation initiation factor
Intein Class Theoretical
Organism Name Pyrococcus furiosus
Organism Description Thermophile, taxon:186497, DSM3638
Domain of Life Archaea
Endonuclease Activity
Endo Motif DOD
Location in Extein K22
Insert Site Comments IF2-a, ATP/GTP-binding site motif A (P-loop)
Intein Size (aa) 387
Intein N-terminal AVLGHVDHGK/C
Intein C-terminal HN/TTLLDRIRKT
Accession No. AAL81261.1 in NCBI/protein, PF1137
Intein aa Sequence >gi|18893214|gb|AAL81261.1|[18893214] Pfu IF2 intein (including -1 and +1 extein residues)
KCLLPEEKVVLPEIGLVTLRELFELANEVVVKDEEKEVRKLGKMLTGVDERGNVKLLNALYVWRVAHK
GEMIRVKVNGWYSVTVTPEHPFLTNRGWVKAGELKEGDYIAIPRRVYGNEDlMKFSKIAKELGIKGDE
KEFYLAGASlDIPIKVLFLAPSKLVSAFLRGYFDAKGVVRENYIEVPLFEDLPLLlLRFGIVSRIEKS
TLKISGKRNLELFRKHVGFTDSEKAKALDELISKAKESERYPIlEELRRLGLLFGFTRNELRIEENPT
YEVlMEILERIERGSPNLAEKIAVLEGRIKEENYLRILEEEGLIENGKLTELGKELLEVWRNREFDSK
DVDYVRNIVENLVFLPVEKVERIEYEGYVYDVTTETHNFVANGILVHNT
Block A CLLPEEKVVLPEI 13
Block B WYSVTVTPEHPFLT 91
Block C LAGASLDIP 158
Block D
Block E FRKHVGFTDS 225
Block H KAKESERYPILEELRRLGL 255
Block F YVYDVTT***ETHNFV 379
Block G NGILVHNT 388
Initially Contributed by Dennis Maeder
Contributor's Address COMB
701 E.Pratt St.
Baltimore MD 21202
Contributor's Phone No. 410-234-8871
Contributor's FAX No. 410-234-8896
Contributor's Email address maeder@umbi.umd.edu
Independently Found By
Comments
Date Submitted 08/28/1998
References Maeder 1999

Last database update: 11/05/10

InBase Home Background Info Splicing mechanism Splicing motifs DOD Endo motifs
Intein registry Intein alleles Selected properties Blast against InBase  
Do you have an intein? Submitting data Bibliography Intein links NEB Home