| Intein Name |
BsuP-SPBc2 RIR1 |
| Prototype Allele |
CIV RIR1 |
| Extein Name |
Ribonucleoside- diphosphate reductase, alpha subunit |
| Intein Class |
Theoretical |
| Organism Name |
B.subtilis strain 168 Sp beta c2 prophage |
| Organism Description |
B.subtilis taxon 1423. SPbeta c2 phage, taxon:66797 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
L380 |
| Insert Site Comments |
RIR1-i |
| Intein Size (aa) |
385 |
| Intein N-terminal |
HISKVKFSNL/C |
| Intein C-terminal |
GN/CSEVLQSSQV |
| Accession No. |
NP_046713.1 in NCBI/protein |
| Intein aa Sequence |
>gi|9630286|ref|NP_046713.1| BsuP-SPBc2 RIR1 intein (including -1 and +1 extein residues)
LCVTGETLLLTENGYEKAADLYKKQNDLKVVIDNRTKDFAVGSKGTTIVDAIPMQLTKKDAEIFKVKTKQ
GYEIRATEWHKFYVKRDGEIQKLQLNQLKTGDKLLVQSAEGAYGKIHEPDLAYIMGIIAGDGTITEKTAK
IYLYDNKKVLEQKVTDAVHRIIQKHKVDRAYKHNTSLLPTFNMANPEKQDLLYMNSTVLFDILKKFGMNK
ETKTRVPEFIFQANKETQAAYLSGLFQTDGCVNANHKAKALTIELTSIHYESLQDVQKLLLNMGVYTTIY
SNNKRSQELLPDGKGGSKLYNVKPTHKISIQDRNSRELFMSIVEMKEYDVYKFNLLTETLQPKSRKPKHD
FTAEIISIEEDGVEDVYDTTQEDYHSLIFNGIVTGNC |
| Block A |
CVTGETLLLTENG 13 |
| Block B |
GYEIRATEWHKFYV 83 |
| Block C |
IMGIIAGDG 131 |
| Block D |
KTRVPEFI 219 |
| Block E |
YLSGLFQTDG 239 |
| Block H |
SIHYESLQDVQKLLLNMGV 274 |
| Block F |
DVYDTTQ**EDYHSLI 377 |
| Block G |
NGIVTGNC 386 |
| Initially Contributed by |
Shmuel Pietrokovski |
| Contributor's Address |
Molecular Genetics Dept.
Weizmann Institute of Science
P.O. Box 26, Rehovot 76100, Israel
http://bioinfo.weizmann.ac.il/~pietro |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
Shmuel.Pietrokovski@weizmann.ac.il |
| Independently Found By |
Vladimir LAZAREVIC (submitted for publication: 9/8/97)
Institut de genetique et de biologie microbiennes
Rue Cesar-Roux 19
1005 LAUSANNE Switzerland
Ph:41 21 3206075 FAX: 41 21 3206078
vladimir.lazarevic@igbm.unil.ch |
| Comments |
This intein was originally submitted to the database by Pietrokovski as a B.subtilis intein, but the data of Lazarevic indicates that it is in the B.subtilis SP beta prophage.
In addition to the intein coding sequence the bnrdE gene contains a Group I self-splicing intron.
Intein penultimate aa is S, not H.
This intein has 2 aa differences compared to the Bsu RIR1 intein from strain M1918. |
| Date Submitted |
11/10/1997 |
| References |
Kunst 1997
Lazarevic 1998
Derbyshire 1998
Ghim 1998
Lazarevic 2001 |
