| Intein Name |
Mtu-H37Rv DnaB |
| Prototype Allele |
Ssp DnaB |
| Extein Name |
DnaB helicase |
| Intein Class |
Experimental |
| Organism Name |
Mycobacterium tuberculosis H37Rv |
| Organism Description |
Human pathogen, taxon:83332 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
G399 |
| Insert Site Comments |
dnaB-a |
| Intein Size (aa) |
416 |
| Intein N-terminal |
PMLADLRESG/C |
| Intein C-terminal |
HN/SLEQDADVVI |
| Accession No. |
CAB02519.1 in NCBI/protein, Rv0058 |
| Intein aa Sequence |
>gi|3250719|emb|CAB02519.1| Mtu-H37Rv DnaB intein (including -1 and +1 extein residues)
GCLTASTRILRADTGAEVAFGELMRSGERPMVWSLDERLRMVARPMINVFPSGRKEVFRLRLASGREVEA
TGSHPFMKFEGWTPLAQLKVGDRIAAPRRVPEPIDTQRMPESELISLARMIGDGSCLKNQPIRYEPVDEA
NLAAVTVSAAHSDRAAIRDDYLAARVPSLRPARQRLPRGRCTPIAAWLAGLGLFTKRSHEKCVPEAVFRA
PNDQVALFLRHLWSAGGSVRWDPTNGQGRVYYGSTSRRLIDDVAQLLLRVGIFSWITHAPKLGGHDSWRL
HIHGAKDQVRFLRHVGVHGAEAVAAQEMLRQLKGPVRNPNLDSAPKKVWAQVRNRLSAKQMMDIQLHEPT
MWKHSPSRSRPHRAEARIEDRAIHELARGDAYWDTVVEITSIGDQHVFDGTVSGTHNFVANGISLHNS |
| Block A |
CLTASTRILRADT 13 |
| Block B |
GREVEATGSHPFMK 77 |
| Block C |
SLARMIGDG 123 |
| Block D |
EKCVPEAV 206 |
| Block E |
FLRHLWSAGG 226 |
| Block H |
STSRRLIDDVAQLLLRVGI 261 |
| Block F |
HVFDGTV**SGTHNFV 408 |
| Block G |
NGISLHNS 417 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
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| Comments |
Identifical intein aa sequence to Mtu strain H37Rv except for 1 change: G153R. Intein activity determined by Eric Adam while in the Perler lab - data not published. |
| Date Submitted |
07/16/1998 |
| References |
Cole 1998
Fleischmann 2002 |
