| Intein Name |
Mja rPol A' |
| Prototype Allele |
|
| Extein Name |
DNA-directed RNA polymerase subunit A' |
| Intein Class |
Experimental |
| Organism Name |
Methanococcus jannaschii (Methanocaldococcus jannaschii DSM 2661) |
| Organism Description |
Thermophile, DSM 2661, taxon:2190 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
V463 |
| Insert Site Comments |
rPol A'-a |
| Intein Size (aa) |
452 |
| Intein N-terminal |
YRTFRHNLCV/C |
| Intein C-terminal |
GN/CPPYNADFDG |
| Accession No. |
A64430 in NCBI/protein, MJ1042 |
| Intein aa Sequence |
>gi|2127864|pir||A64430| Mja rPol A' intein (including -1 and +1 extein
residues)
VCVDGDTTVLLDGKLIKIKDLEDKWKDVKVLTSDDLNPKLTSLSKYWKLNADEYGKKIYKIKTELGREII
ATEDHPFYTTNGRKRCGELKVGDEVIIYPNDFPMFEDDNRVIVDEEKIKKVINNIGGTYKNKIINELKDR
KLIPLTYNDQKASILARIVGHVMGDGSLIINNKNSRVVFRGDIEDLKTIKEDLKELGYDGEEIKLHEGET
EITDYNGKKRIIKGKGYSFEVRKKSLCILLKALGCVGGDKTKKMYGIPNWIKTAPKYIKKEFLSAYFGSE
LTTPKIRNHGTSFKELSFKIAKIEEIFDEDRFIKDIKEMLKEFGIELKVRVEEGNLRKDGYKTKVYVASI
YNHKEFFGRIGYTYANKKETLARYAYEYLLTKEKYLKDRNIKKLENNTKFITFDKFIKEKCLKNGFVKEK
IVSIEETKVDYVYDITTISETHNFIANGFLTGNC |
| Block A |
CVDGDTTVLLDGK 13 |
| Block B |
GREIIATEDHPFYT 78 |
| Block C |
--------- |
| Block D |
RKLIPLTY 146 |
| Block E |
RIVGHVMGDG 165 |
| Block H |
YSFEVRKKSLCILLKALGC 244 |
| Block F |
YVYDITT*ISETHNFI 444 |
| Block G |
NGFLTGNC 453 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
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| Comments |
Intein penultimate aa is G, not H (Perler). |
| Date Submitted |
|
| References |
Bult 1996
Chen 2000 |
