| Intein Name |
Tfus RecA-2 |
| Prototype Allele |
Mle-TN RecA |
| Extein Name |
RecA |
| Intein Class |
Theoretical |
| Organism Name |
Thermobifida fusca YX |
| Organism Description |
Thermophile,taxon:269800 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
G649 |
| Insert Site Comments |
recA-b |
| Intein Size (aa) |
357 |
| Intein N-terminal |
LREKVGVMFG/C |
| Intein C-terminal |
HN/SPETTSGGRA |
| Accession No. |
YP_288864 in NCBI/protein, Tfu0803 |
| Intein aa Sequence |
>gi|72161207|ref|YP_288864.1|[72161207] Tfus RecA-2 intein (including -1 and +1 extein residues)
GCMHYDTLVTLADGTQEKIGTIVDRKLDVEVLSYDPETDRIVPRRVVNWFDNGAADHFLQ
FTVGRSGKPGGAQFTATPNHLIRTPGGWREAGELIAGDRVLVHEPHYLNEQQRQVVYGSL
MGRGTLVPDRHGGPGVHFCMAHTAEQAAYLDWKVSLLGNIAHSRTAEASATVGVEFTPMP
ELSELHRVVDFGDGHTHLTWEFLKQLTPLALAVWYLDAGTLTIPQSGTDDDARVQIDVET
LSPGSRQRLVEYLRDTHELDAAVVQQGADARSLLEFTPAATVRFLELVAPYVPESMSSML
LAQFRGRCSVTPEYSDPVQRLVAAPVLDIQVKPGSTRKFDIEVEGNHNYFVDGVMVHNS |
| Block A |
CMHYDTLVTLADG 13 |
| Block B |
GAQFTATPNHLIRT 83 |
| Block C |
VYGSLMGRG 123 |
| Block D |
-------- |
| Block E |
LHRVVDFGDG 193 |
| Block H |
HLTWEFLKQLTPLALAVWY 214 |
| Block F |
RKFDIEV**EGNHNYF 349 |
| Block G |
DGVMVHNS 358 |
| Initially Contributed by |
Janos Posfai |
| Contributor's Address |
New England Biolabs, Inc. |
| Contributor's Phone No. |
1-978-927-5054 |
| Contributor's FAX No. |
1-978-921-1350 |
| Contributor's Email address |
|
| Independently Found By |
|
| Comments |
The organism was formerly called Thermomonaspora fusca, a soil bacterium (growth temperature 55°C).
Note similarity between the sequences preceding BOTH splice junctions: vGVMfg/c vs. dGVMvhn/s. |
| Date Submitted |
10/22/2001 |
| References |
|
|
