| Intein Name |
Tko Pol-1 (Pko Pol-1) |
| Prototype Allele |
Pko Pol-1 |
| Extein Name |
DNA polymerase (alpha or family B) |
| Intein Class |
Experimental |
| Organism Name |
Pyrococcus/ Thermococcus kodakaraensis KOD1 |
| Organism Description |
Thermophile, taxon:69014 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
PI-PkoI |
| Endo Motif |
DOD |
| Location in Extein |
R406 |
| Insert Site Comments |
pol-a, Pol Motif A |
| Intein Size (aa) |
360 |
| Intein N-terminal |
WENIVYLDFR/C |
| Intein C-terminal |
HN/SLYPSIIITH |
| Accession No. |
S71551 in NCBI/protein |
| Intein aa Sequence |
>gi|2129415|pir||S71551| P77933 Tko Pol-1 (Psp-KOD Pol-1) intein (including -1 and +1 extein residues)
RCHPADTKVVVKGKGIINISEVQEGDYVLGIDGWQRVRKVWEYDYKGELVNINGLKCTPNHKLPVVTKNE
RQTRIRDSLAKSFLTKKVKGKIITTPLFYEIGRATSENIPEEEVLKGELAGILLAEGTLLRKDVEYFDSS
RKKRRISHQYRVEITIGKDEEEFRDRITYIFERLFGITPSISEKKGTNAVTLKVAKKNVYLKVKEIMDNI
ESLHAPSVLRGFFEGDGSVNRVRRSIVATQGTKNEWKIKLVSKLLSQLGIPHQTYTYQYQENGKDRSRYI
LEITGKDGLILFQTLIGFISERKNALLNKAISQREMNNLENNGFYRLSEFNVSTEYYEGKVYDLTLEGTP
YYFANGILTHNS |
| Block A |
CHPADTKVVVKGK 13 |
| Block B |
INGLKCTPNHKLPV 64 |
| Block C |
LAGILLAEG 126 |
| Block D |
VKEIMDNI 209 |
| Block E |
VLRGFFEGDG 226 |
| Block H |
TKNEWKIKLVSKLLSQLGI 259 |
| Block F |
KVYDLTL**EGTPYYF 352 |
| Block G |
NGILTHNS 361 |
| Initially Contributed by |
F.Perler for Tadayuki Imanaka |
| Contributor's Address |
Osaka University
Dept. of Biotechnology
2-1 Yamadaoka, Suita, Osaka 565
Japan |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
|
| Independently Found By |
|
| Comments |
Formally called Pko Pol-1, Psp Pol-2 or Psp KOD Pol-1.
This organism is now classified as Thermococcus kodakaraensis. |
| Date Submitted |
07/08/1996 |
| References |
Takagi 1997
Nishioka 1998
Hashimoto 2001
Fukui 2005 |
