| Intein Name |
Mja RtcB (Mja Hyp-2) |
| Prototype Allele |
Mja RtcB |
| Extein Name |
RNA terminal phosphate cyclase operon orfB |
| Intein Class |
Theoretical |
| Organism Name |
Methanococcus jannaschii (Methanocaldococcus jannaschii DSM 2661) |
| Organism Description |
Thermophile, DSM 2661, taxon:2190 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
N97 |
| Insert Site Comments |
rtcB-a |
| Intein Size (aa) |
488 |
| Intein N-terminal |
SPGGVGFDIN/C |
| Intein C-terminal |
HN/CGVRLIRTNL |
| Accession No. |
Q58095 in NCBI/protein, MJ0682 |
| Intein aa Sequence |
>gi|2501617|sp|Q58095| Mja RtcB (Mja Hyp-2) intein (including -1 and +1 extein residues)
NCLTSNSKILTDDGYYIKLEKLKEKLDLHIKIYNTEEGEKSSNILFVSERYADEKIIRIKTESGRVLEGS
KDHPVLTLNGYVPMGMLKEGDDVIVYPYEGVEYEEPSDEIILDEDDFAEYDKQIIKYLKDRGLLPLRMDN
KNIGIIARLLGFAFGDGSIVKENGDRERLYVAFYGKRETLIKIREDLEKLGIKASRIYSRKREVEIRNAY
GDEYTSLCEDNSIKITSKAFALFMHKLGMPIGKKTEQIYKIPEWIKKAPKWVKRNFLAGLFGADGSRAVF
KNYTPLPINLTMSKSEELKENILEFLNEIKLLLAEFDIESMIYEIKSLDGRVSYRLAIVGEESIKNFLGR
INYEYSGEKKVIGLLAYEYLRRKDIAKEIRKKCIKRAKELYKKGVTVSEMLKMDEFRNEFISKRLIERAV
YENLDEDDVRISTKFPKFEEFIEKYGVIGGFVIDKIKEIEEISYDSKLYDVGIVSKEHNFIANSIVVHNC |
| Block A |
CLTSNSKILTDDG 13 |
| Block B |
GRVLEGSKDHPVLT 76 |
| Block C |
LLGFAFGDG 156 |
| Block D |
IYKIPEWI 254 |
| Block E |
FLAGLFGADG 274 |
| Block H |
ENILEFLNEIKLLLAEFDI 317 |
| Block F |
KLYDVGI*VSKEHNFI 480 |
| Block G |
NSIVVHNC 489 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
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| Comments |
Formally called Hyp-2 intein. In E.coli this gene is in an operon with rtcA - an RNA 3'-terminal phosphate cyclase - and rtcR the sigma-54 regulator of rtcA and rtcB (Pietrokovski, Genschik 98). |
| Date Submitted |
|
| References |
Bult 1996
Genschik 1997
Genschik 1998 |
