| Intein Name |
Kla-CBS683 VMA |
| Prototype Allele |
Sce VMA |
| Extein Name |
Vacuolar ATPase (H+-transporting ATP synthase), subunit A |
| Intein Class |
Theoretical |
| Organism Name |
Kluyveromyces lactis, strain CBS683 |
| Organism Description |
Yeast, taxon:28985 |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
G18? |
| Insert Site Comments |
VMA-a |
| Intein Size (aa) |
410 |
| Intein N-terminal |
SNSDAIIYVG/C |
| Intein C-terminal |
HN/CGERGNEMAE |
| Accession No. |
CAC86345.1 in NCBI/protein |
| Intein aa Sequence |
>gi|27526577|emb|CAC86345.1|[27526577] Kla-CBS683 VMA intein (including -1 and +1 extein residues)
CFSKGTEVMMGDGKDELIENIQVGDEVMGRDGLPRQVVGLPRGHDDMYQVTEKSEDNET
AKISFQCNSSHKLVLVTPQDIRLTESKEKVTVAFNRLADISVGNGTESRTVRLVERAEKS
FANAESNQAIINAAEFVTTIDTTSIEWTLEARDMLLVHSSIREVTQQLINPVLLEKEHLA
GVLKSNDFQSSLAPQFSYLLGAFVGSSGKDNSKYLQQLSAQFDKKIVAEKSIDVQSNGKT
VGTASIVISQEPVQENKRRKVAQVSLVSKIVQESFSSGIPSFMMSENINVRESFLAGIVD
SQNQKLDDTVALKTLSVKTHDGIARLARSLGIRVSGKKQNQEYTLTLSGDALKSVSNWTS
TSNIEKVDVIHKAQPISFDLEKIESADYFGVTLAEESDHKFLLSNMTLVHNC |
| Block A |
CFSKGTEVMMGDG 13 |
| Block B |
KISFQCNSSHKLVL 74 |
| Block C |
LLGAFVGSS 206 |
| Block D |
SSGIPSFM 282 |
| Block E |
FLAGIVDSQN 302 |
| Block H |
TLSVKTHDGIARLARSLGI 330 |
| Block F |
FGVTLA**EESDHKFL 401 |
| Block G |
NMTLVHNC 411 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
Koufopanou V., Biology, Imperial College at Silwood Park, Ascot, Berks, SL5 7PY, UNITED KINGDOM |
| Comments |
Okuda 2003 indicates endo is inactive. |
| Date Submitted |
04/28/2003 |
| References |
Koufopanou 2002
Okuda 2003 |
|
