| Intein Name |
Pho RFC |
| Prototype Allele |
Mja RFC-1 |
| Extein Name |
Replication factor C |
| Intein Class |
Theoretical |
| Organism Name |
Pyrococcus horikoshii OT3 |
| Organism Description |
Thermophile, taxon:53953 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
K61 |
| Insert Site Comments |
RFC-a, ATP/GTP-binding site motif A (P-loop) |
| Intein Size (aa) |
525 |
| Intein N-terminal |
LFAGPPGVGK/C |
| Intein C-terminal |
HN/TTAALALSRE |
| Accession No. |
F71231 in NCBI/protein, PH0112 |
| Intein aa Sequence |
>gi|7521348|pir||F71231| Pho RFC intein (including -1 and +1 extein residues)
KCLTGDTKVIANGQLFELRELVEKISGGKFGPTPVKGLKVIGIDEDGKLREFEVQYVYKDKTERLIRIRT
RLGRELKVTPYHPLLVNRRNGEIKWVKAEELKPGDKLAVPRFLPIVTGEDPLAEWLGYFLGGGYADSKEN
LIMFTNEDPLLRQRFMELTEKLFSDARIREITHENGTSKVYVNSKKALKLVNSLGNAHIPKECWRGIRSF
LRAYFDCNGGVKGNAIVLATASKEMSQEIAYALAGFGIISRIQEYRVIISGSDNVKKFLNEIGFINRNKL
EKALKLVKKDDPGHDGLEINYELISYVKDRLRLSFFNDKRSWSYREAKEISWELMKEIYYRLDELEKLKE
SLSRGILIDWNEVAKRIEEVAEETGIRADELLEYIEGKRKLSFKDYIKIAKVLGIDVEHTIEAMRVFARK
YSSYAEIGRRLGTWNSSVKTILESNAVNVEILERIRKIELELIEEILSDEKLKEGIAYLIFLSQNELYWD
EITKVEELRGEFIIYDLHVPGYHNFIAGNMPTVVHNT |
| Block A |
CLTGDTKVIANGQ 13 |
| Block B |
GRELKVTPYHPLLV 85 |
| Block C |
WLGYFLGGG 132 |
| Block D |
NAHIPKEC 202 |
| Block E |
FLRAYFDCNG 218 |
| Block H |
TASKEMSQEIAYALAGFGI 247 |
| Block F |
IIYDLHV**PGYHNFI 515 |
| Block G |
MPTVVHNT 526 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
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| Comments |
Intein insertion site numbered using Met1 as listed in above Acc. No.
Motifs by Pietrokovski and Perler |
| Date Submitted |
07/20/1998 |
| References |
Kawarabayasi 1998
Kawarabayasi 1998B
Kawarabayasi 2001 |
