| Intein Name |
Tac-ATCC25905 VMA |
| Prototype Allele |
Pho VMA |
| Extein Name |
Vacuolar ATPase (H+-transporting ATP synthase), subunit A |
| Intein Class |
Experimental |
| Organism Name |
Thermoplasma acidophilum, ATCC 25905 |
| Organism Description |
Thermophile, taxon:2303 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
None |
| Location in Extein |
K235 |
| Insert Site Comments |
VMA-b, ATP/GTP-binding site motif A (P-loop) |
| Intein Size (aa) |
173 |
| Intein N-terminal |
RVPGPFGSGK/C |
| Intein C-terminal |
HN/TVIQHQLAKW |
| Accession No. |
AF286477_1 in NCBI/protein, TVN0052 |
| Intein aa Sequence |
>gi|9502270|gb|AAF88065.1|AF286477_1| Tac-ATCC25905 VMA intein (including -1 and +1 extein residues)
KCVSGDTPVLLDAGERRIGDLFMEAIRPKERGEIGQNEEIVRLHDSWRIYSMVGSEIVETVSHAIYHGKS
NAIVNVRTENGREVRVTPVHKLFVKIGNSVIERPASEVNEGDEIAWPSVSENGDSQTVTTTLVLTFDRVV
SKEMHSGVFDVYDLMVPDYGYNFIGGNGLIVLHNT |
| Block A |
CVSGDTPVLLDAG 13 |
| Block B |
GREVRVTPVHKLFV 92 |
| Block C |
None |
| Block D |
None |
| Block E |
None |
| Block H |
None |
| Block F |
DVYDLMV*PDYGYNFI 163 |
| Block G |
GLIVLHNT 174 |
| Initially Contributed by |
J. Peter Gogarten and Elena Hilario |
| Contributor's Address |
J. Peter Gogarten
Depar.Mol. & Cell Biology, Univ. of Connecticut
75 N. Eagleville Rd
Storrs CT 06269-3044, and Elena Hilario (see below) |
| Contributor's Phone No. |
JPG: 1-860-846-4061/ EH:64-9-815-4200 x7024 |
| Contributor's FAX No. |
|
| Contributor's Email address |
gogarten@uconnvm.uconn.edu |
| Independently Found By |
Elena Hilario (hilarioe@landcare.cri.nz)
Landcare Research
Priv Bag 92-170
120 Mt Albert Rd.
Mt Albert, Auckland, New Zealand |
| Comments |
The blocks were identified by Dr Shmuel Pietrokovski. |
| Date Submitted |
12/15/1999 |
| References |
Ruepp 2000
Senejani 2001 |
