| Intein Name |
Pho Pol-II |
| Prototype Allele |
Pho Pol-II |
| Extein Name |
DNA polymerase II, DP2 subunit |
| Intein Class |
Theoretical |
| Organism Name |
Pyrococcus horikoshii OT3 |
| Organism Description |
Thermophile, taxon:53953 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
None |
| Location in Extein |
N954 |
| Insert Site Comments |
pol_II-a |
| Intein Size (aa) |
166 |
| Intein N-terminal |
PYFHAAKRRN/C |
| Intein C-terminal |
HQ/CDGDEDAVML |
| Accession No. |
G71232 in NCBI/protein, PH0121 |
| Intein aa Sequence |
>gi|7518477|pir||G71232| Pho Pol-II intein (including -1 and +1 extein residues)
NCFPGDTRILVQINGTPQRVTLKELYELFDEEHYESMVYVRKKPKVDIKVYSFNPEEGKVVLTDIEEVIK
APATDHLIRFELELGSSFETTVDHPVLVYENGKFVEKRAFEVREGNIIIIIDESTLEPLKVAVKKIEFIE
PPEDFVFSLNAKKYHTVIINENIVTHQC |
| Block A |
CFPGDTRILVQIN 13 |
| Block B |
GSSFETTVDHPVLV 97 |
| Block C |
None |
| Block D |
None |
| Block E |
None |
| Block H |
None |
| Block F |
FVFSLNA**KKYHTVI 157 |
| Block G |
ENIVTHQC 167 |
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
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| Contributor's Phone No. |
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| Contributor's FAX No. |
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| Contributor's Email address |
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| Independently Found By |
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| Comments |
This gene is part of a 2 subunit archaeal DNA pol that may be the replicative pol. Intein insertion site numbered using Met1 as listed in above Acc. No. Has a Gln instead of Asn in Block G.
This pol is specific to Archaea. |
| Date Submitted |
07/16/1998 |
| References |
Kawarabayasi 1998
Kawarabayasi 1998B
Kawarabayasi 2001
Shen 2001
Shen 2003B
Shen 2004
Shen 2004B
Tang 2004 |
