| Intein Name |
Tfus RecA-1 |
| Prototype Allele |
Pho RadA |
| Extein Name |
RecA |
| Intein Class |
Theoretical |
| Organism Name |
Thermobifida fusca YX |
| Organism Description |
Thermophile,taxon:269800 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
K95 |
| Insert Site Comments |
recA-c, ATP/GTP-binding site motif A (P-loop) |
| Intein Size (aa) |
422 |
| Intein N-terminal |
EIYGPESSGK/C |
| Intein C-terminal |
HN/TTVALHAVAN |
| Accession No. |
YP_288864 in NCBI/protein, Tfu0803 |
| Intein aa Sequence |
>gi|72161207|ref|YP_288864.1|[72161207] Tfus RecA-1 intein (including -1 and +1 extein residues)
KCLTADTYVWTDRGLETVAEVFGRAGLPLSSTSRVTDVRDRDIRVVNEKGELEQVAALTH
NGRQPVVRITVASGRQVTVTRNHPLRVMNDDGFIVWREAGQLREGDVLVSAAFGAVQAAS
GGGLSEDEAVLLGYLTAAGSLDPAGHVCFTTTDIETGAEFAALAEWLLDTTVTAVPGDGQ
VAYVLSDPAARHTLAERYGVDYAAAARIPQCVRTAGDKMQRAFLAALYTAAGWTDTSAAV
GLRTASAPLAREVQYLLYGLGIPADLDRSHGNGQHPWAVTISPAAAPRFHTEVGFRTAQQ
SPQTGLHEPTPQVEAIPNLTGLIHALRDSIGDRAESTDDPFPAASGGAYDRDQVRRVIDW
AKRRTDEAPATANAILGYLTQLTDARYTYEPITAVEDAGQQPTFDLMVPRTHSFLANGIL
SHNT |
| Block A |
CLTADTYVWTDRG 13 |
| Block B |
GRQVTVTRNHPLRV 86 |
| Block C |
LLGYLTAAG 138 |
| Block D |
AARIPQCV 211 |
| Block E |
FLAALYTAAG 231 |
| Block H |
TASAPLAREVQYLLYGLGI 261 |
| Block F |
PTFDLMV**PRTHSFL 414 |
| Block G |
NGILSHNT 423 |
| Initially Contributed by |
Janos Posfai |
| Contributor's Address |
New England Biolabs, Inc. |
| Contributor's Phone No. |
1-978-927-5054 |
| Contributor's FAX No. |
1-978-921-1350 |
| Contributor's Email address |
|
| Independently Found By |
|
| Comments |
The organism was formerly called Thermomonaspora fusca, a soil bacterium (growth temperature 55°C).
Alternative Block C: AGQLREGDV 106 |
| Date Submitted |
10/22/2001 |
| References |
|
|
