New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
Contact NEB About Us Site Map Request a Catalog OEM at NEB ISO International Orders Freezer Program Quick Order
ShP-Sfv-5 Primase Intein
Intein Name ShP-Sfv-5 Primase
Prototype Allele ShP-Sfv-5 Primase
Extein Name DNA primase, Helicase
Intein Class Theoretical
Organism Name Shigella flexneri 5 str. 8401
Organism Description Bacteriphage,isolation_source_epidemic, taxon:373384
Domain of Life Eubacteria
Endonuclease Activity
Endo Motif DOD
Location in Extein K371
Insert Site Comments primase-a, ATP/GTP-binding site motif A (P-loop)
Intein Size (aa) 342
Intein N-terminal LVNGVNGHGK/A
Intein C-terminal HN/TEVVGHMALE
Accession No. ABF04780 in NCBI/protein, SFV_2689
Intein aa Sequence >gi|110616113|gb|ABF04780.1|[110616113] ShP-Sfv-5 Primase intein (including -1 and +1 extein residues)
KACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAELPDYNFSG
DGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELRNG
VVQLVNSLGGSCRTRVKTGVTYTYKDDKQHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIGNAE
CLCIMVDHPRHLYVTRGYVATHNT
Block A ACPLNEPILLADG 13
Block B GRYVDCAGDHLWEV 69
Block C VIGSLLGDG 128
Block D NKFIPRVF 199
Block E MLCGLLETDG 219
Block H SASEELRNGVVQLVNSLGG 249
Block F ECLCIMV*DHPRHLYV 333
Block G GYVATHNT 343
Initially Contributed by Fran Perler
Contributor's Address
Contributor's Phone No.
Contributor's FAX No.
Contributor's Email address
Independently Found By
Comments
Date Submitted 10/23/2006
References Nie 2006
Wei 2003

Last database update: 11/05/10

InBase Home Background Info Splicing mechanism Splicing motifs DOD Endo motifs
Intein registry Intein alleles Selected properties Blast against InBase  
Do you have an intein? Submitting data Bibliography Intein links NEB Home