| Intein Name |
AP-APSE1 dpol |
| Prototype Allele |
AP-APSE1 dpol |
| Extein Name |
DNA polymerase A (Pol I or family A) |
| Intein Class |
Theoretical |
| Organism Name |
Acyrthosiphon pisum secondary endosymbiot phage 1 |
| Organism Description |
Bacteriophage, taxon:67571 |
| Domain of Life |
Eubacteria |
| Endonuclease Activity |
|
| Endo Motif |
None |
| Location in Extein |
S608 |
| Insert Site Comments |
dpol-a |
| Intein Size (aa) |
306 |
| Intein N-terminal |
ERLKTYGGKS/C |
| Intein C-terminal |
HN/CENICQAAAR |
| Accession No. |
AAF03988.1 in NCBI/protein, P45 |
| Intein aa Sequence |
>gi|6118040|gb|AAF03988.1|AF157835_45| AAF03988.1 P45| AP-APSE1 dpol intein (including -1 and +1 extein residues)
SCLAKGTLVLTITGWMPIEIVSQDAYVWDGIEWVRTDGSVFNGNQEVIQAYGVGMTADHQVLTEKGWKSA
SQSKRYNRSSCRLPDGYKLPRFRRKEINLESTLHLWTRNNHSSNRITKTKKTRYNCLLRMPKGTNNIMQK
PKARNVKTPRFCCMEQHVSQMYSPFPQSMVKLWWSGNNGLQTLAKKFQQFLGRHGQDIPTRLIFRSHQQQ
CRLPPQKLPLGYVASTSSKYSTSTIRANSPRHNEYTGISSPNRDCSKHALLSPGKKGKSSTTSGAPKHIA
EVYDLINCGPRNRFVIATPDGPLIVHNC |
| Block A |
CLAKGTLVLTITG 13 |
| Block B |
AYGVGMTADHQVLT 62 |
| Block C |
None |
| Block D |
None |
| Block E |
None |
| Block H |
None |
| Block F |
EVYDLIN*CGPRNRFV 294 |
| Block G |
GPLIVHNC 307 |
| Initially Contributed by |
Shmuel Pietrokovski |
| Contributor's Address |
Molecular Genetics Dept.
Weizmann Institute of Science
PO Box 26
Rehovot 76100
Israel |
| Contributor's Phone No. |
972 (8) 934 2747 |
| Contributor's FAX No. |
972 (8 934 4108 |
| Contributor's Email address |
Shmuel.Pietrokovski@weizmann.ac.il |
| Independently Found By |
|
| Comments |
First intein in DNA polymerase I. Inteins are now known in the catalytic subunits of all major families of DNA polymerases - Pol type A (this intein), type B (archaeal pol inteins), type C (Synechocystis polC split intein) and archaeal DP2 (also called type D, archaeal polC gene). Integration site is well conserved only in bacteriophage pol-I sequences. (Pietrokovski) |
| Date Submitted |
12/01/1999 |
| References |
van der Wilk 1999 |
