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AP-APSE1 dpol Intein
Intein Name AP-APSE1 dpol
Prototype Allele AP-APSE1 dpol
Extein Name DNA polymerase A (Pol I or family A)
Intein Class Theoretical
Organism Name Acyrthosiphon pisum secondary endosymbiot phage 1
Organism Description Bacteriophage, taxon:67571
Domain of Life Eubacteria
Endonuclease Activity
Endo Motif None
Location in Extein S608
Insert Site Comments dpol-a
Intein Size (aa) 306
Intein N-terminal ERLKTYGGKS/C
Intein C-terminal HN/CENICQAAAR
Accession No. AAF03988.1 in NCBI/protein, P45
Intein aa Sequence >gi|6118040|gb|AAF03988.1|AF157835_45| AAF03988.1 P45| AP-APSE1 dpol intein (including -1 and +1 extein residues)
SCLAKGTLVLTITGWMPIEIVSQDAYVWDGIEWVRTDGSVFNGNQEVIQAYGVGMTADHQVLTEKGWKSA
SQSKRYNRSSCRLPDGYKLPRFRRKEINLESTLHLWTRNNHSSNRITKTKKTRYNCLLRMPKGTNNIMQK
PKARNVKTPRFCCMEQHVSQMYSPFPQSMVKLWWSGNNGLQTLAKKFQQFLGRHGQDIPTRLIFRSHQQQ
CRLPPQKLPLGYVASTSSKYSTSTIRANSPRHNEYTGISSPNRDCSKHALLSPGKKGKSSTTSGAPKHIA
EVYDLINCGPRNRFVIATPDGPLIVHNC
Block A CLAKGTLVLTITG 13
Block B AYGVGMTADHQVLT 62
Block C None
Block D None
Block E None
Block H None
Block F EVYDLIN*CGPRNRFV 294
Block G GPLIVHNC 307
Initially Contributed by Shmuel Pietrokovski
Contributor's Address Molecular Genetics Dept.
Weizmann Institute of Science
PO Box 26
Rehovot 76100
Israel
Contributor's Phone No. 972 (8) 934 2747
Contributor's FAX No. 972 (8 934 4108
Contributor's Email address Shmuel.Pietrokovski@weizmann.ac.il
Independently Found By
Comments First intein in DNA polymerase I. Inteins are now known in the catalytic subunits of all major families of DNA polymerases - Pol type A (this intein), type B (archaeal pol inteins), type C (Synechocystis polC split intein) and archaeal DP2 (also called type D, archaeal polC gene). Integration site is well conserved only in bacteriophage pol-I sequences. (Pietrokovski)
Date Submitted 12/01/1999
References van der Wilk 1999

Last database update: 11/05/10

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