| Intein Name |
Sce VMA |
| Prototype Allele |
Sce VMA |
| Extein Name |
Vacuolar ATPase (H+-transporting ATP synthase), subunit A |
| Intein Class |
Experimental |
| Organism Name |
Saccharomyces cerevisiae (nucleus) |
| Organism Description |
Yeast, also in Sce strains OUT7163, OUT7045, OUT7163, IFO1992 |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
PI-SceI |
| Endo Motif |
DOD |
| Location in Extein |
G283 |
| Insert Site Comments |
VMA-a |
| Intein Size (aa) |
454 |
| Intein N-terminal |
SNSDAIIYVG/C |
| Intein C-terminal |
HN/CGERGNEMAE |
| Accession No. |
PXBYVA in NCBI/protein |
| Intein aa Sequence |
>gi|67951|pir||PXBYVA | Sce VMA intein (including -1 and +1 extein residues)
GCFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRETMYSVVQKSQHRAHKSDSSREVPE
LLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERA
NELVESYRKASNKAYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLA
YLLGLWIGDGLSDRATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRGNGIRNN
LNTENPLWDAIVGLGFLKDGVKNIPSFLSTDNIGTRETFLAGLIDSDGYVTDEHGIKATIKTIHTSVRDG
LVSLARSLGLVVSVNAEPAKVDMNGTKHKISYAIYMSGGDVLLNVLSKCAGSKKFRPAPAAAFARECRGF
YFELQELKEDDYYGITLSDDSDHQFLLANQVVVHNC |
| Block A |
CFAKGTNVLMADG 13 |
| Block B |
LLKFTCNATHELVV 83 |
| Block C |
LLGLWIGDG 219 |
| Block D |
VKNIPSFL 307 |
| Block E |
FLAGLIDSDG 327 |
| Block H |
TIHTSVRDGLVSLARSLGL 359 |
| Block F |
YGITLS**DDSDHQFL 445 |
| Block G |
NQVVVHNC 455 |
| Initially Contributed by |
Yasuhiro Anraku |
| Contributor's Address |
Depart. of Biological Sciences
Graduate School of Science
University of Tokyo
Hongo 7-3-1, Bunkyo-ku, Tokyo 113, Japan |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
anraku@uts2.s.u-tokyo.ac.jp |
| Independently Found By |
Tom Stevens, Univerisity of Oregon
Norma Neff, NY Blood Ctr
Jeremy Thorner, UC Berkley
Fred Gimble, Texas A&M |
| Comments |
Sce VMA is a nuclear gene. An identical intein aa sequence is present in strain Out7163, (BAC66646.1), OUT7045 (BAC6664), OUT7163 (BAC66646),IFO1992 (BAC66650). Also see AAA34664 NCBI/protein. |
| Date Submitted |
|
| References |
Shih 1988
Hirata 1990
Kane 1990
Hirata 1992
Bremer 1992
Ryan 1992
Gimble 1992
Cooper 1993
Gimble 1993
Anraku 1994
Gimble 1995
Gimble 1996
Chong 1996
Kawasaki 1996
Wende 1996
Chong 1997
Chong 1997B
Duan 1997
Kawasaki 1997
Kawasaki 1997B
Nogami 1997
He 1998
Chong 1998
Grindl 1998
Muir 1998
Pingoud 1998
Severinov 1998
Chong 1998B
Cotton 1999
Xu 1999
Yu 1999
Pingoud 1999
Hu 1999
Miyamoto 1999
Hu 2000
Poland 2000
Gimble 2001
Mizutani 2002
Posey 2002
Morassutti 2002
Moure 2002
Werner 2002
Nogami 2002
Okuda 2003
Fukuda 2003
Gimble 2003
Steuer 2004
Mizutani 2004
Anraku 2005 |
