| Intein Name |
Neq Pol-n |
| Prototype Allele |
Tli Pol-2 |
| Extein Name |
DNA polymerase (alpha or family B) |
| Intein Class |
Experimental |
| Organism Name |
Nanoarchaeum equitans Kin4-M |
| Organism Description |
Thermophile, taxon:228908 |
| Domain of Life |
Archaea |
| Endonuclease Activity |
|
| Endo Motif |
None |
| Location in Extein |
D579 |
| Insert Site Comments |
pol-c, Pol Motif C |
| Intein Size (aa) |
98 |
| Intein N-terminal |
EEGFKVIYGD/S |
| Intein C-terminal |
HN/TDSLFISGDK |
| Accession No. |
NP_963362.1 in NCBI/protein, NEQ068 |
| Intein aa Sequence |
>gi|41614864|ref|NP_963362.1|[41614864] Neq Pol-n N-terminal intein fragment (including -1 extein residue)
DSIMDTEIEVIENGIKKKEKLSDLFNKYYAGFQIGEKHYAFPPDLYVYDG
ERWVKVYSIIKHETETDLYEINGITLSANHLVLSKGNWVKAKEYENKNN |
| Block A |
SIMDTEIEVIENG N13 |
| Block B |
INGITLSANHLVLS N83 |
| Block C |
None |
| Block D |
None |
| Block E |
None |
| Block H |
None |
| Block F |
|
| Block G |
|
| Initially Contributed by |
Fran Perler |
| Contributor's Address |
|
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
|
| Independently Found By |
|
| Comments |
The Neq Pol intein is the second example of a naturally split intein potentially capable of protein trans-splicing. |
| Date Submitted |
03/23/2004 |
| References |
Waters 2003
Choi 2006 |
