| Intein Name |
Sas RPB2 |
| Prototype Allele |
Cst RPB2 |
| Extein Name |
DNA-directed RNA polymerase II, second largest subunit |
| Intein Class |
Theoretical |
| Organism Name |
Spiromyces aspiralis NRRL 22631 |
| Organism Description |
Zygomycete fungus, isolate="AFTOL-ID 185",taxon:68401 |
| Domain of Life |
Eucarya |
| Endonuclease Activity |
|
| Endo Motif |
DOD |
| Location in Extein |
H697? |
| Insert Site Comments |
RPB2-b, domain 6, H1104 in Sce RPB2 |
| Intein Size (aa) |
354 |
| Intein N-terminal |
LKHMVDDKIH/C |
| Intein C-terminal |
GN/SRARGPV--- |
| Accession No. |
ABC17955.1 in NCBI/protein |
| Intein aa Sequence |
>gi|83415495|gb|ABC17955.1|[83415495] Sas RPB2 intein (including -1 and +1 extein residues)
VCLTPDHEVLTERGWRPIADLALDDRVATLQEGVRLSYEQPRKLFAYDYVGPMYHIENDHVSLITTPNHRMWTSISAAPG
DKCQFGLRRADEIIGKQVRYQKDVSSWDQSDYQLLDALGVGVDPTDRSVMTVIGGCLLGRGLATYPLVAVEVAGPPQGLA
ILAEAARKLGLQAETTADGALAVRGAEALLDLCKNALVEDAGARQFPHWVWKLSSSQCRQLLDGICIVHGKRLYGSGNDA
AMTTYVPVASKHLADDIQRLCLHAASGVIANIKPLSLPAASQGAAETPGQQPQLWTVEIATGFEKCLPEFNQADIEQRES
QVELLEMYAGKVHCIEVPGHVFYVRHNGKAVWTGNS |
| Block A |
CLTPDHEVLTERG 13 |
| Block B |
HVSLITTPNHRMWT 72 |
| Block C |
IGGCLLGRG 140 |
| Block D |
ARQFPHWV 209 |
| Block E |
|
| Block H |
VASKHLADDIQRLCLHAAS 265 |
| Block F |
KVHCIEV***PGHVFY 342 |
| Block G |
KAVWTGNS 355 |
| Initially Contributed by |
Tim Goodwin |
| Contributor's Address |
Department of Biochemistry
University of Otago
710 Cumberland Street
Dunedin,New Zealand |
| Contributor's Phone No. |
|
| Contributor's FAX No. |
|
| Contributor's Email address |
timg@sanger.otago.ac.nz |
| Independently Found By |
|
| Comments |
Insertion site listed for partial extein sequence.
Insertion site at equivalent position of H1104 in Sce RPB2 CAA99357. |
| Date Submitted |
04/09/2006 |
| References |
|
